Single Nucleotide Polymorphisms in MicroRNA Binding Sites: Implications in Colorectal Cancer

Cancer is a complex genetic disorder, characterised by uncontrolled cell proliferation and caused by altered expression of oncogenes and tumour suppressor genes. When cell proliferation pertains to colon, it is called colorectal cancer. Most of colorectal cancer causing genes are potential targets for the miRNA (microRNA) that bind to 3′UTR (untranslated regions) of mRNA and inhibit translation. Mutations occurring in miRNA binding regions can alter the miRNA, mRNA combination, and can alter gene expression drastically. We hypothesized that 3′UTR mutation in miRNA binding site could alter the miRNA, mRNA interaction, thereby altering gene expression. Altered gene expression activity could promote tumorigenesis in colon. Therefore, we formulated a systematic in silico procedure that integrates data from various databases, followed rigorous selection criteria, and identified mutations that might alter the expression levels of cancer causing genes. Further we performed expression analysis to shed light on the potential tissues that might be affected by mutation, enrichment analysis to find the metabolic functions of the gene, and network analysis to highlight the important interactions of cancer causing genes with other genes to provide insight that complex network will be disturbed upon mutation. We provide in silico evidence for the effect of these mutations in colorectal cancer

Electric and magnetic polarizabilities of hexagonal Ln2CuTiO6 (Ln=Y, Dy, Ho, Er and Yb)

We investigated the rare-earth transition metal oxide series, Ln2CuTiO6 (Ln=Y, Dy, Ho, Er and Yb), crystallizing in the hexagonal structure with non-centrosymmetric P63cm space group for possible occurrences of multiferroic properties. Our results show that while these compounds, except Ln=Y, exhibit a low temperature antiferromagnetic transition due to the ordering of the rare-earth moments, the expected ferroelectric transition is frustrated by the large size difference between Cu and Ti at the B-site. Interestingly, this leads these compounds to attain a rare and unique combination of desirable paraelectric properties with high dielectric constants, low losses and weak temperature and frequency dependencies. First-principles calculations establish these exceptional properties result from a combination of two effects. A significant difference in the MO5 polyhedral sizes for M = Cu and M = Ti suppress the expected co-operative tilt pattern of these polyhedra, required for the ferroelectric transition, leading to relatively large values of the dielectric constant for every compound investigated in this series. Additionally, it is shown that the majority contribution to the dielectric constant arises from intermediate-frequency polar vibrational modes, making it relatively stable against any temperature variation. Changes in the temperature stability of the dielectric constant amongst different members of this series are shown to arise from changes in relative contributions from soft polar modes.Comment: Accepted for publication in Phys. Rev. B (21 pages, 2 Table, 8 Figures

Needle(s) in the Haystack – Synchronous Multifocal Tumor Induced Osteomalacia

This is the author accepted manuscript. The final version is available from Endocrine Society via http://dx.doi.org/10.1210/jc.2015-3854MG is supported by the NIHR Cambridge Biomedical Research Centre

Gene Regulatory Network Rewiring in the Immune Cells Associated with Cancer

The gene regulatory networks (GRNs) of immune cells not only indicate cell identity but also reveal the dynamic changes of immune cells when comparing their GRNs. Cancer immunotherapy has advanced in the past few years. Immune-checkpoint blockades (i.e., blocking PD-1, PD-L1, or CTLA-4) have shown durable clinical effects on some patients with various advanced cancers. However, major gaps in our knowledge of immunotherapy have been recognized. To fill these gaps, we conducted a systematic analysis of the GRNs of key immune cell subsets (i.e., B cell, CD4, CD8, CD8 naïve, CD8 Effector memory, CD8 Central Memory, regulatory T, Thelper1, Thelper2, Thelp17, and NK (Nature killer) and DC (Dendritic cell) cells associated with cancer immunologic therapies. We showed that most of the GRNs of these cells in blood share key important hub regulators, but their subnetworks for controlling cell type-specific receptors are different, suggesting that transformation between these immune cell subsets could be fast so that they can rapidly respond to environmental cues. To understand how cancer cells send molecular signals to immune cells to make them more cancer-cell friendly, we compared the GRNs of the tumor-infiltrating immune T cells and their corresponding immune cells in blood. We showed that the network size of the tumor-infiltrating immune T cells’ GRNs was reduced when compared to the GRNs of their corresponding immune cells in blood. These results suggest that the shutting down certain cellular activities of the immune cells by cancer cells is one of the key molecular mechanisms for helping cancer cells to escape the defense of the host immune system. These results highlight the possibility of genetic engineering of T cells for turning on the identified subnetworks that have been shut down by cancer cells to combat tumors

Challenges in Microbial Fuel Cell and Future Scope

The imminent energy crisis due to reserved fossil fuel and global warming due to greenhouse gas emission warrant the necessity for environmentally friendly sources of energy. Fossil fuels adversely affect the nature due to the release of CO2 into the environment (Venkata Mohan et al. 2011; Venkateswar Reddy et al. 2011a; Kadier et al. 2016a, b). Hence the consumption of fossil fuels based energy sources has harshly threatened human life through its drastic consequences, such as global warming and environmental pollution. As a result, in the present global energy scenario, searching for other energy resources is necessary (Kadier et al. 2015; Chandrasekhar et al. 2015a; Venkata Mohan and Pandey 2013). Last few years, several researchers around the world have made remarkable efforts to find a solution for this energy crisis (Venkata Mohan et al. 2013). In this scenario, fuel cells are a unique addition to the list of alternative energy sources having a negligible CO2 emission. Production of electricity using microorganisms was firstly reported early in the last century (Potter 1911). Microbial fuel cell (MFC) have been considered as a bio-based reactor that modifies the chemical energy of substrate into electrical energy through biocatalytic action of exoelectrogenic microorganisms under anaerobic circumstances over sequences of metabolic reactions (Kondaveeti and Min 2015; Chandrasekhar et al. 2015b). MFC technology signifies an innovative approach of using microorganisms for bioelectricity generation by the oxidation of organic substrate varied from the synthetic substrate such as acetate, glucose to a complex mixture of the organic substrate including food, dairy, distillery, animal and domestic wastewater. In recent years, MFC technology has been developing as one of the popular wastewater treatment based technology to deliver clean water and green energy (Venkata Mohan and Chandrasekhar 2011a, b; Pant et al. 2012; Pandit et al. 2012a, b; Chandrasekhar et al. 2015b). These MFCs overtook other conventional technologies such as an aerated lagoon and anaerobic digester (Logan 2008). Unlike traditional fuel cells, one step conversion of carbon-rich organic waste as a potential substrate to generate bioelectricity in MFCs ensures better conversion capability. MFCs can evade extra gas treatment process due to its CO2 rich off-gas. Moreover, single chambered or open-air cathode MFCs do not need any external energy input. Hence, it can be advantageous for extensive application in locations lacking electrical amenities (Stams et al. 2006). This chapter stretches an account of the basic principles involved in the working of MFCs and the key applications, challenges and future scope of MFC technology, as it stands today

Cadaveric position of unilateral vocal cord: a case of cricoid fracture with ipsilateral arytenoid dislocation

We report a case of cricoid cartilage fracture with unilateral arytenoid dislocation following a motorcycle accident. This 25 year old male sustained blunt injury to the head, face and neck. He presented late to the hospital with one week history of dysphonia. Laryngoscopy revealed cadaveric position of the non-functioning left vocal cord. CT and MRI showed laterally displaced left vocal cord. Displaced fractures were noted in the cricoid at the junction of lamina with the anterior arch on the left side and at the right side of the anterior arch, along with dislocated left arytenoid resulting in ipsilateral vocal cord palsy. Medialization thyroplasty was performed to improve his phonation. Laryngeal trauma warrants close monitoring because of the risk of airway compromise. Radiologists play a crucial role in early diagnosis and should always have high index of suspicion. Recognition of laryngeal injury is important for initial resuscitation as well as for long term airway and vocal function

A comprehensive review on two-stage integrative schemes for the valorization of dark fermentative effluents

This review provides the alternative routes towards the valorization of dark H2 fermentation effluents that are mainly rich in volatile fatty acids such as acetate and butyrate. Various enhancement and alternative routes such as photo fermentation, anaerobic digestion, utilization of microbial electrochemical systems, and algal system towards the generation of bioenergy and electricity and also for efficient organic matter utilization are highlighted. What is more, various integration schemes and two-stage fermentation for the possible scale up are reviewed. Moreover, recent progress for enhanced performance towards waste stabilization and overall utilization of useful and higher COD present in the organic source into value-added products are extensively discussed