Einfluss der Insektenherbivorie auf den Kreislauf fester und gelöster organischer Substanzen in einem Grasslandökosystem - eine Mesokosmenstudie

Insektenmassenvermehrungen können durch die Reduktion von oberirdischer Biomasse (z.B. Blattverlust) und Koteintrag Einfluss auf den Kreislauf der organischen Substanz in Ökosystemen nehmen und dadurch zeitlich und räumlich begrenzte massive Veränderungen biogeochemischer Reaktionsraten hervorrufen. Um die Auswirkungen auf den C- und N-Kreislauf in Grasländern im System Herbivore-Pflanze-Boden zu quantifizieren, wurde ein Mesokosmenexperiment (D: 50 cm, H: 100 cm) mit Knäuelgras (Dactylis glomerata) und Heuschrecken (Chorthippus dorsatus) durchgeführt. Mit Hilfe von C-13-O2-Gas und N-15 markiertem Kot (δN-15: 58‰) wurde der C- und N-Pfad in den Kompartimenten Blatt, Wurzel, Grashüpfer, Kot, Boden und in der Eintrags- und Bodenlösung verfolgt. Es wurden folgende vier Varianten durchgeführt (n=3): Kontrolle; C-13-O2; C-13-O2+20_Grashüpfer; C-13-O2+20_Grashüpfer+N-15-markierter Kot (+9.2 µg N*cm-2). Innerhalb einer 5-Tage-Inkubation wurden die Kosmen bewässert (13 mm/5d). Nach fünf Tagen wurden die Bodenlösungen und die Kaltwasser- sowie mikrobielle Biomasse-Extrakte (CFE) (0-4, 4-12 cm) auf gelösten OC, OδC-13 und N untersucht. Ebenfalls wurden TOC, δC-13, TN sowie δN-15 Werte aller Kompartimente bestimmt. Nach fünf Tagen waren sowohl die Grashüpfer, der Kot als auch die Eintragslösung des Kotes signifikant mit C-13 angereichert. C-13 wurde stärker in Wurzeln als in Blätter eingebaut (anhand δC-13-Werten). Erhöhte DOC-13 Werte im Boden weisen auf einen schnellen Blatt-C-Kreislauf über Grashüpfer, Kot bis in die Bodenlösung, die Bodenorganismen und die Graswurzeln hin. Dies wird ebenfalls durch den zugegebenen N-15 Kot deutlich, welcher um 91% seines Gesamt-N-Gehaltes reduziert wurde. Es scheint eine schnelle N-Freisetzung durch Auswaschung und Wurzelaufnahme (-0.82±0.28‰ vs -1.54±0.12‰) stattgefunden zu haben. N-15 war im frisch produzierten Kot (0.62±0.4‰ vs -0.14 ±0.27‰) aber nicht in den Blättern angereichert. Die Herbivorie hatte keinen Einfluss auf die N-Menge in der Bodenlösung und in den Extrakten, was eine schnelle Pflanzenaufnahme des freigewordenen N vermuten lässt. Die geringe N-Konzentration des Mineralbodens (0.14%) und der Bodenlösungen (1-2.3 mg*L-1) weisen im Allgemeinen auf eine geringe Pflanzen-N-Verfügbarkeit hin. Wir schließen daraus, dass innerhalb des kurzen zeitlichen Umfanges (5d) N-limitierte Weidesysteme robust auf starken Insektenbefall reagieren und nicht zu signifikanten, ökosystemaren N-Auswaschungsverlusten neigen

Identification of heat shock protein 32 (Hsp32) as a novel target in acute lymphoblastic leukemia

Heat shock proteins (Hsp) are increasingly employed as therapeutic targets in oncology. We have shown that Hsp32, also known as heme oxygenase-1 (HO-1), serves as survival factor and potential target in Ph+ chronic myeloid leukemia. We here report that primary cells and cell lines derived from patients with acute lymphoblastic leukemia (ALL) express Hsp32 mRNA and the Hsp32 protein in a constitutive manner. Highly enriched CD34+/CD38- ALL stem cells also expressed Hsp32. Two Hsp32-targeting drugs, pegylated zinc protoporphyrine (PEG-ZnPP) and styrene maleic acid-micelle-encapsulated ZnPP (SMA-ZnPP), induced apoptosis and growth arrest in the BCR/ABL1+ cell lines, in Ph- lymphoblastic cell lines and in primary Ph+ and Ph- ALL cells. The effects of PEG-ZnPP and SMA-ZnPP on growth of leukemic cells were dose-dependent. In Ph+ ALL, major growth-inhibitory effects of the Hsp32-targeting drugs were observed in imatinib-sensitive and imatinib-resistant cells. Hsp32-targeting drugs were found to synergize with imatinib, nilotinib, and bendamustine in producing growth inhibition and apoptosis in Ph+ ALL cells. A siRNA against Hsp32 was found to inhibit growth and survival of ALL cells and to synergize with imatinib in suppressing the growth of ALL cells. In conclusion, Hsp32 is an essential survival factor and potential new target in ALL.Sabine Cerny-Reiterer, Renata A. Meyer, Harald Herrmann, Barbara Peter, Karoline V. Gleixner, Gabriele Stefanzl, Emir Hadzijusufovic, Winfried F. Pickl, Wolfgang R. Sperr, Junia V. Melo, Hiroshi Maeda, Ulrich Jäger, Peter Valen

Measurement of Atmospheric Neutrino Oscillations with the ANTARES Neutrino Telescope

The data taken with the ANTARES neutrino telescope from 2007 to 2010, a total live time of 863 days, are used to measure the oscillation parameters of atmospheric neutrinos. Muon tracks are reconstructed with energies as low as 20 GeV. Neutrino oscillations will cause a suppression of vertical upgoing muon neutrinos of such energies crossing the Earth. The parameters determining the oscillation of atmospheric neutrinos are extracted by fitting the event rate as a function of the ratio of the estimated neutrino energy and reconstructed flight path through the Earth. Measurement contours of the oscillation parameters in a two-flavour approximation are derived. Assuming maximum mixing, a mass difference of $\Delta m_{32}^2=(3.1\pm 0.9)\cdot 10^{-3}$ eV$^2$ is obtained, in good agreement with the world average value.Comment: 9 pages, 5 figure

Search for muon-neutrino emission from GeV and TeV gamma-ray flaring blazars using five years of data of the ANTARES telescope

The ANTARES telescope is well-suited for detecting astrophysical transient neutrino sources as it can observe a full hemisphere of the sky at all times with a high duty cycle. The background due to atmospheric particles can be drastically reduced, and the point-source sensitivity improved, by selecting a narrow time window around possible neutrino production periods. Blazars, being radio-loud active galactic nuclei with their jets pointing almost directly towards the observer, are particularly attractive potential neutrino point sources, since they are among the most likely sources of the very high-energy cosmic rays. Neutrinos and gamma rays may be produced in hadronic interactions with the surrounding medium. Moreover, blazars generally show high time variability in their light curves at different wavelengths and on various time scales. This paper presents a time-dependent analysis applied to a selection of flaring gamma-ray blazars observed by the FERMI/LAT experiment and by TeV Cherenkov telescopes using five years of ANTARES data taken from 2008 to 2012. The results are compatible with fluctuations of the background. Upper limits on the neutrino fluence have been produced and compared to the measured gamma-ray spectral energy distribution.Comment: 27 pages, 16 figure

The Antares Collaboration : Contributions to the 34th International Cosmic Ray Conference (ICRC 2015, The Hague)

The ANTARES detector, completed in 2008, is the largest neutrino telescope in the Northern hemisphere. Located at a depth of 2.5 km in the Mediterranean Sea, 40 km off the Toulon shore, its main goal is the search for astrophysical high energy neutrinos. In this paper we collect the 21 contributions of the ANTARES collaboration to the 34th International Cosmic Ray Conference (ICRC 2015). The scientific output is very rich and the contributions included in these proceedings cover the main physics results, ranging from steady point sources, diffuse searches, multi-messenger analyses to exotic physics

Systems-pharmacology dissection of a drug synergy in imatinib-resistant CML

Occurrence of the BCR-ABL[superscript T315I] gatekeeper mutation is among the most pressing challenges in the therapy of chronic myeloid leukemia (CML). Several BCR-ABL inhibitors have multiple targets and pleiotropic effects that could be exploited for their synergistic potential. Testing combinations of such kinase inhibitors identified a strong synergy between danusertib and bosutinib that exclusively affected CML cells harboring BCR-ABL[superscript T315I]. To elucidate the underlying mechanisms, we applied a systems-level approach comprising phosphoproteomics, transcriptomics and chemical proteomics. Data integration revealed that both compounds targeted Mapk pathways downstream of BCR-ABL, resulting in impaired activity of c-Myc. Using pharmacological validation, we assessed that the relative contributions of danusertib and bosutinib could be mimicked individually by Mapk inhibitors and collectively by downregulation of c-Myc through Brd4 inhibition. Thus, integration of genome- and proteome-wide technologies enabled the elucidation of the mechanism by which a new drug synergy targets the dependency of BCR-ABL[superscript T315I] CML cells on c-Myc through nonobvious off targets

Search for muon neutrinos from gamma-ray bursts with the ANTARES neutrino telescope using 2008 to 2011 data

9 pages, 8 figures; added Fig. 1 with effective area, updated Fig. 8 (b) according to arXiv:1204.4219 ; Références publication Astron Astrophys 559 (2013) A9International audienceAims. We search for muon neutrinos in coincidence with GRBs with the ANTARES neutrino detector using data from the end of 2007 to 2011. Methods. Expected neutrino fluxes were calculated for each burst individually. The most recent numerical calculations of the spectra using the NeuCosmA code were employed, which include Monte Carlo simulations of the full underlying photohadronic interaction processes. The discovery probability for a selection of 296 GRBs in the given period was optimised using an extended maximum-likelihood strategy. Results. No significant excess over background is found in the data, and 90% confidence level upper limits are placed on the total expected flux according to the model

A search for neutrino emission from the Fermi bubbles with the ANTARES telescope

Analysis of the Fermi-LAT data has revealed two extended structures above and below the Galactic Centre emitting gamma rays with a hard spectrum, the so-called Fermi bubbles. Hadronic models attempting to explain the origin of the Fermi bubbles predict the emission of high-energy neutrinos and gamma rays with similar fluxes. The ANTARES detector, a neutrino telescope located in the Mediterranean Sea, has a good visibility to the Fermi bubble regions. Using data collected from 2008 to 2011 no statistically significant excess of events is observed and therefore upper limits on the neutrino flux in TeV range from the Fermi bubbles are derived for various assumed energy cutoffs of the source

A search for time dependent neutrino emission from microquasars with the ANTARES telescope

Results are presented on a search for neutrino emission from a sample of six microquasars, based on the data collected by the ANTARES neutrino telescope between 2007 and 2010. By means of appropriate time cuts, the neutrino search has been restricted to the periods when the acceleration of relativistic jets was taking place at the microquasars under study. The time cuts have been chosen using the information from the X-ray telescopes RXTE/ASM and Swift/BAT, and, in one case, the gamma-ray telescope Fermi/LAT. No statistically significant excess has been observed, thus upper limits on the neutrino fluences have been derived and compared to the predictions by models. Constraints have been put on the ratio of proton to electron luminosity in the jets

Searches for Point-like and extended neutrino sources close to the Galactic Centre using the ANTARES neutrino Telescope

A search for cosmic neutrino sources using six years of data collected by the ANTARES neutrino telescope has been performed. Clusters of muon neutrinos over the expected atmospheric background have been looked for. No clear signal has been found. The most signal-like accumulation of events is located at equatorial coordinates R.A. = -46 degrees.8 and decl. = -64 degrees.9 and corresponds to a 2.2 sigma background fluctuation. In addition, upper limits on the flux normalization of an E-2 muon neutrino energy spectrum have been set for 50 pre-selected astrophysical objects. Finally, motivated by an accumulation of seven events relatively close to the Galactic Center in the recently reported neutrino sample of the IceCube telescope, a search for point sources in a broad region around this accumulation has been carried out. No indication of a neutrino signal has been found in the ANTARES data and upper limits on the flux normalization of an E-2 energy spectrum of neutrinos from point sources in that region have been set. The 90% confidence level upper limits on the muon neutrino flux normalization vary between 3.5 and 5.1 x 10(-8) GeV cm(-2) s(-1), depending on the exact location of the source