227 research outputs found
Strains of Paramecium quadecaurelia from Namibia, Africa; Genetic and Molecular Studies
Summary. New strains of Paramecium quadecaurelia were found in Namibia, Africa. Previously, this species from the P. aurelia complex was known only from Australia, Emily Gap. Namibian strains were identified by mating reaction; their relationship with the Australian strain was studied by classical strain crosses (survival in F1 and F2 generations) and by comparison of cytosol-type hsp70 gene sequences. Phylogenetic trees of the Namibian and Australian strains of P. quadecaurelia and the other species of the P. aurelia complex were generated based on the maximum-likelihood method
Rare Freshwater Ciliate Paramecium chlorelligerum Kahl, 1935 and Its Macronuclear Symbiotic Bacterium "Candidatus Holospora parva"
Ciliated protists often form symbioses with many diverse microorganisms. In particular, symbiotic associations between ciliates and green algae, as well as between ciliates and intracellular bacteria, are rather wide-spread in nature. In this study, we describe the com- plex symbiotic system between a very rare ciliate, Paramecium chlorelligerum, unicellular algae inhabiting its cytoplasm, and novel bacteria colonizing the host macronucleus. Para- mecium chlorelligerum, previously found only twice in Germany, was retrieved from a novel location in vicinity of St. Petersburg in Russia. Species identification was based on both clas- sical morphological methods and analysis of the small subunit rDNA. Numerous algae occu- pying the cytoplasm of this ciliate were identified with ultrastructural and molecular methods as representatives of the Meyerella genus, which before was not considered among symbi- otic algae. In the same locality at least fifteen other species of “green” ciliates were found, thus it is indeed a biodiversity hot-spot for such protists. A novel species of bacterial symbi- onts living in the macronucleus of Paramecium chlorelligerum cells was morphologically and ultrastructurally investigated in detail with the description of its life cycle and infection capabilities. The new endosymbiont was molecularly characterized following the full-cycle rRNA approach. Furthermore, phylogenetic analysis confirmed that the novel bacterium is a member of Holospora genus branching basally but sharing all characteristics of the genus except inducing connecting piece formation during the infected host nucleus division. We propose the name “Candidatus Holospora parva” for this newly described species. The described complex system raises new questions on how these microorganisms evolve and interact in symbiosis
The neotypification of Frontonia vernalis (Ehrenberg, 1833) Ehrenberg, 1838 and the description of Frontonia paravernalis sp. nov. trigger a critical revision of frontoniid systematics
Background: Among Oligohymenophorea (Ciliophora, Alveolata) the subclass Peniculia stands as one of the most
well-known groups. Frontonia is the largest genus of Peniculia, and its representatives are spread in any type of
water bodies as well as in soil. At a first glance, Frontonia species exhibit an overall similar morphology, and form a
well-recognizable taxon of ciliates. Despite the general morphological homogeneity, the phylogenetic analysis
based on the 18S rDNA sequencing showed that Frontonia is a non-monophyletic group. The systematics of this
genus should be deeply reviewed, although additional issues complicate the task solving. First, type species of the
genus is not yet clearly established, and no type material is available. In this context, the situation of F. vernalis, one
of the first Frontonia ever described, is somehow puzzled: the description of this ciliate made by Ehrenberg (in 1833
and 1838) contains several inaccuracies and subsequent misidentifications by other authors occurred. Moreover, the
18S rDNA sequence of a putative F. vernalis is available on GenBank, but no morphological description of the
correspondent specimens is provided; thus, in our opinion, it should be only prudently associated with F. vernalis or
at least indicated as “F. vernalis”.
Results: In the present work, we provide the neotypification of F. vernalis newly found in Italy, presenting its
multidisciplinary description and its neotype material. Similarly, we describe a novel species bearing Chlorella-like
endosymbionts, Frontonia paravernalis sp. nov., retrieved in two far distant locations (Italy, Russia). A critical
discussion on the status of Frontonia taxonomy and phylogeny is also presented, based on the 18S rDNA
sequencing of both these two newly collected species and other 14 frontoniids isolated in different parts of the
world. Finally, in the present study F. leucas was neotypified and proposed as the type species of the genus.
Conclusions: Green frontoniids form a monophyletic clade of freshwater organisms characterized by having a
single contractile vacuole and bearing intracytoplasmatic Chlorella-like symbionts. With the neotypification of F.
vernalis and F. leucas a fundamental step in Frontonia systematics was taken, and the bases for further taxonomic
studies were laid
Morphology, ultrastructure, and molecular phylogeny of the ciliate Sonderia vorax with insights into the systematics of order Plagiopylida
BACKGROUND: Ciliates of the family Sonderiidae are common members of the eukaryotic communities in various anoxic environments. They host both ecto- and endosymbiotic prokaryotes (the latter associated with hydrogenosomes) and possess peculiar morpho-ultrastructural features, whose functions and homologies are not known. Their phylogenetic relationships with other ciliates are not completely resolved and the available literature, especially concerning electron microscopy and molecular studies, is quite scarce. RESULTS: Sonderia vorax Kahl, 1928 is redescribed from an oxygen-deficient, brackish-water pond along the Ligurian Sea coastlines of Italy. Data on morphology, morphometry, and ultrastructure are reported. S. vorax is ovoid-ellipsoid in shape, dorsoventrally flattened, 130 x 69 μm (mean in vivo); it shows an almost spherical macronucleus, and one relatively large micronucleus. The ventral kinetom has a “secant system” including fronto-ventral and fronto-lateral kineties. A distinctive layer of bacteria laying between kineties covers the ciliate surface. Two types of extrusomes and hydrogenosomes-endosymbiotic bacteria assemblages are present in the cytoplasm. The phylogeny based on 18S rRNA gene sequences places S. vorax among Plagiopylida; Sonderiidae clusters with Plagiopylidae, although lower-level relationships remain uncertain. The studied population is fixed as neotype and the ciliate is established as type species of the genus, currently lacking. CONCLUSIONS: This is the first description of a representative of Sonderiidae performed with both morphological and molecular data. To sum up, many previous hypotheses on this interesting, poorly known taxon are confirmed but confusion and contradictory data are as well highlighted
Paramecium tredecaurelia: A Unique Non-Polymorphic Species of the P. aurelia spp. Complex (Oligohymenophorea, Ciliophora)
New stands of Paramecium tredecaurelia, a rare species of the P. aurelia spp. complex, were identified in Thailand and Madagascar on the basis of mating reactions and molecular markers (rDNA and mtDNA). Analysis of DNA fragments showed that all P. tredecaurelia strains, the recently recorded ones and the ones known previously from France, Mexico, and Israel, form a monophyletic and well-defined clade in the P. aurelia species trees. All of these strains, collected from different localities around the world, represent identical or nearly identical haplotypes in terms of all the studied DNA fragments. Given the huge distances between particular collection sites, such a low level of variability of the studied sequences may result from a slow rate of evolution in P. tredecaurelia
The ALICE experiment at the CERN LHC
ALICE (A Large Ion Collider Experiment) is a general-purpose, heavy-ion detector at the CERN LHC which focuses on QCD, the strong-interaction sector of the Standard Model. It is designed to address the physics of strongly interacting matter and the quark-gluon plasma at extreme values of energy density and temperature in nucleus-nucleus collisions. Besides running with Pb ions, the physics programme includes collisions with lighter ions, lower energy running and dedicated proton-nucleus runs. ALICE will also take data with proton beams at the top LHC energy to collect reference data for the heavy-ion programme and to address several QCD topics for which ALICE is complementary to the other LHC detectors. The ALICE detector has been built by a collaboration including currently over 1000 physicists and engineers from 105 Institutes in 30 countries. Its overall dimensions are 161626 m3 with a total weight of approximately 10 000 t. The experiment consists of 18 different detector systems each with its own specific technology choice and design constraints, driven both by the physics requirements and the experimental conditions expected at LHC. The most stringent design constraint is to cope with the extreme particle multiplicity anticipated in central Pb-Pb collisions. The different subsystems were optimized to provide high-momentum resolution as well as excellent Particle Identification (PID) over a broad range in momentum, up to the highest multiplicities predicted for LHC. This will allow for comprehensive studies of hadrons, electrons, muons, and photons produced in the collision of heavy nuclei. Most detector systems are scheduled to be installed and ready for data taking by mid-2008 when the LHC is scheduled to start operation, with the exception of parts of the Photon Spectrometer (PHOS), Transition Radiation Detector (TRD) and Electro Magnetic Calorimeter (EMCal). These detectors will be completed for the high-luminosity ion run expected in 2010. This paper describes in detail the detector components as installed for the first data taking in the summer of 2008
Centrality evolution of the charged-particle pseudorapidity density over a broad pseudorapidity range in Pb-Pb collisions at root s(NN)=2.76TeV
Peer reviewe
Bütschli, O
Otto Bütschli (1848–1920) – professor of zoology
and palaeontology at Heidelberg University, made
fundamental contributions to cytology and the
investigation of unicellular organisms, first of all
ciliates. Bütschli was the first to recognise and to
demonstrate that conjugation of ciliates was not
a reproductive process but a sexual reorganisation
of the cell similar to cross-fertilisation. His
protozoan studies culminated in a three-volume
monograph (Bütschli, 1880–1882, 1883–1887,
1887–1889), which also contained many of his
own original works. This 2000-page critical review
of contemporary knowledge actually established
protozoology as an autonomous discipline. Moreover,
in 1875, Bütschli was the first to identify and
determine the sequence of the stages of nuclear
division (mitosis) in several types of animal cells.
He provided excellent descriptions of dividing
cells
Resistance of Paramecium Species (Ciliophora, Peniculia) to Salinity of Environment
Summary The salinity resistance and tolerance of 10 species from the genus Paramecium were investigated. The species investigated could be divided into groups in according with their resistance. These groups partly corresponded to the classification of the genus proposed earlier on the basis of morphological features. The ciliates from the"woodruffi" group were more resistant than paramecia from the "aurelia" group. P. bursaria and P. putrinum (the former "putrinum" group) have lower salinity resistance and tolerance than the species from two other groups. P. polycaryum has a salinity resistance and tolerance which corresponded to its "intermediate" position between the "aurelia" and the "woodruffi" groups
- …