Projeto político e pedagógico anti racista na educação infantil na escola municipal do campo de João Surá

Orientador: Adalberto Penha de PaulaMonografia (especialização) - Universidade Federal do Paraná, Setor Litoral, Curso de Especialização em Educação do CampoInclui referênciasResumo: A pesquisa Projeto político e pedagógico anti racista na educação infantil na Escola Municipal do Campo de João Surá do Quilombo de João Surá, com o histórico de luta pelos direitos da terra de seus antepassados e melhores condições de vida para os moradores. Com cunho qualitativo, neste cenário buscamos analisar importância da construção do Projeto Político Pedagógico da Escola Municipal do Campo de João Surá para que atenda os elementos fundamentais da educação Quilombola. Tendo um compromisso de debater as questões em diálogo com a comunidade a partir das teorias da infância e da Educação do Campo, visando a vivência comunitária, e a luta em busca da superação do racismo institucional no ambiente escolar. Os procedimentos metodológicos adotados foram qualitativa, buscando analisar questões históricas e políticas da região sobre a infância a partir das considerações das lideranças. Com os objetivos específicos queremos: a) Evidenciar a conquista histórica da Escola Municipal do campo João Surá no território quilombola; b) Buscar na LDB e na legislação municipal, os marcos da Educação Infantil e Ensino Fundamenta I; c) Propor algumas estratégias para construção do projeto político pedagógico considerando a realidade da comunidade quilombola e as contradições encontradas

Leaf Transcriptome Of Two Highly Divergent Genotypes Of Urochloa Humidicola (poaceae), A Tropical Polyploid Forage Grass Adapted To Acidic Soils And Temporary Flooding Areas

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Urochloa humidicola (Koronivia grass) is a polyploid (6x to 9x) species that is used as forage in the tropics. Facultative apospory apomixis is present in most of the genotypes of this species, although one individual has been described as sexual. Molecular studies have been restricted to molecular marker approaches for genetic diversity estimations and linkage map construction. The objectives of the present study were to describe and compare the leaf transcriptome of two important genotypes that are highly divergent in terms of their phenotypes and reproduction modes: the sexual BH031 and the aposporous apomictic cultivar BRS Tupi. Results: We sequenced the leaf transcriptome of Koronivia grass using an Illumina GAIIx system, which produced 13.09 Gb of data that consisted of 163,575,526 paired-end reads between the two libraries. We de novo-assembled 76,196 transcripts with an average length of 1,152 bp and filtered 35,093 non-redundant unigenes. A similarity search against the non-redundant National Center of Biotechnology Information (NCBI) protein database returned 65 % hits. We annotated 24,133 unigenes in the Phytozome database and 14,082 unigenes in the UniProtKB/Swiss-Prot database, assigned 108,334 gene ontology terms to 17,255 unigenes and identified 5,324 unigenes in 327 known metabolic pathways. Comparisons with other grasses via a reciprocal BLAST search revealed a larger number of orthologous genes for the Panicum species. The unigenes were involved in C4 photosynthesis, lignocellulose biosynthesis and flooding stress responses. A search for functional molecular markers revealed 4,489 microsatellites and 560,298 single nucleotide polymorphisms (SNPs). A quantitative real-time PCR analysis validated the RNA-seq expression analysis and allowed for the identification of transcriptomic differences between the two evaluated genotypes. Moreover, 192 unannotated sequences were classified as containing complete open reading frames, suggesting that the new, potentially exclusive genes should be further investigated. Conclusion: The present study represents the first whole-transcriptome sequencing of U. humidicola leaves, providing an important public information source of transcripts and functional molecular markers. The qPCR analysis indicated that the expression of certain transcripts confirmed the differential expression observed in silico, which demonstrated that RNA-seq is useful for identifying differentially expressed and unique genes. These results corroborate the findings from previous studies and suggest a hybrid origin for BH031.17Brazilian National Council for Scientific and Technological Development (CNPq) [478262/2004-3, 502336/2005-6, 482458/2007-0]Computational Biology Program from Coordination of Superior Level Staff Improvement [CAPES 15/2013]State of Sao Paulo Research Foundation (FAPESP) [2008/52197-4]FAPESP [2013/14903-2, 2013/20447-0]CNPq [150719/2015-9, 307430/2007-3]Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Pedagogical Practices in an Educational Territory of Priority Intervention

This study follows the theoretical line of various authors, among them Abelha, Machado, Costa Lobo (2014), Roldão (1999a, 1999b, 2007), Hargreaves (1998), and Alarcão (2003), and examines collaborative work among the teachers of D. Pedro I School Cluster, in North Portugal. This school cluster is part of the Educational Territories of Priority Intervention. We have used a quantitative methodological design to analyze the teachers’ different opinions and representations of the dynamics of collaborative work, their conceptions of curriculum, curriculum management, curriculum development, and educational projects. The information was subjected to statistical analysis, with the support of Statistical Package for Social Sciences (SPSS), version 22, a calculation software tool. Given the results obtained, we have been faced with the scope of the initial purpose of this study, that is, examining respondents’ understanding of the concepts listed above, how they manage the curriculum, the characterization of work dynamics, and the evaluation of the changes regarding the teacher satisfaction about work dynamics according to the personal and professional characteristics of the respondents. In short, this cluster can be considered an example of good collaborative practices, with effective management, and leadership that supports, encourages and motivates its teachers. This study opens up potential future scenarios of research and intervention in the field of reflective school, focusing on school cultures of excellence.Este estudo segue a linha teórica de diversos autores, entre eles, Abelha, Machado e Costa Lobo (2014), Roldão (1999a, 1999b, 2007a), Hargreaves (1998) e Alarcão (2003), e cumpre o propósito de estudar o trabalho colaborativo entre os docentes do Agrupamento de Escolas D. Pedro I, no norte de Portugal. Esse Agrupamento, no qual uma das autoras deste trabalho exerce funções docentes, faz parte dos Territórios Educativos de Intervenção Prioritária (TEIP). Utilizamos um design metodológico do tipo quantitativo, que permitiu estudar as diferentes opiniões dos professores pertencentes ao contexto educativo em estudo, acerca das suas representações sobre as dinâmicas de trabalho colaborativo e das suas concepções sobre conceitos como currículo, gestão curricular, desenvolvimento curricular e projeto educativo. As informações recolhidas foram submetidas a análises estatísticas, com o apoio da ferramenta informática de cálculo Statistical Package for the Social Sciencies (SPSS), versão 22. Perante os resultados obtidos, somos então confrontados com o alcance do objetivo inicial deste estudo, isto é, a compreensão da apropriação dos respondentes relativamente aos conceitos já elencados, bem como a compreensão da gestão que fazem do currículo, a caracterização das dinâmicas de trabalho e a avaliação das alterações manifestas quanto à satisfação dos docentes com a dinâmica de trabalho, mediante as características pessoais e profissionais dos inquiridos. Em suma, este Agrupamento pode ser considerado um exemplo de boas práticas colaborativas, com uma gestão eficaz e uma liderança que apoia, incentiva e motiva os seus docentes. Esta investigação deixa em aberto potenciais cenários futuros de investigação e intervenção no domínio da escola reflexiva, privilegiando as culturas escolares de referência

DEPRESSION SYMPTOMS IN NURSING PROFESSIONALS DURING THE COVID-19 PANDEMIC

Objetivo: identificar sintomas de depressão em profissionais de enfermagem durante a pandemia de covid-19.Método: estudo transversal e observacional, via formulário eletrônico, nas cinco regiões do Brasil, entre profissionais de enfermagem. Utilizou-se instrumento com informações gerais e o Patient Health Questionnaire-9 para identificar sintomas de depressão. Adotaram-se testes de hipóteses t de Student e Análise de Variância.Resultados: participaram 3249 profissionais. Destes, 2092 (64,4%) não apresentaram ou apresentaram sintomas mínimos de depressão, 603 (18,6%) moderados, 330 (10,2%) sintomas de moderado a severos e 224 (6,9%) sintomas severos. Mulheres, trabalhadores da região Norte, adultos jovens, solteiros e com renda de até quatro salários apresentaram escore de depressão mais elevados (p<0,05).Conclusão: os profissionais de enfermagem não apresentaram ou apresentaram sintomas leves de depressão. Variáveis como sexo, faixa etária, estado civil, região do país, ter contato com pessoas com covid-19 e não usar máscaras tiveram diferenças significativas com sintomas de depressão.Objective: to identify depression symptoms in Nursing professionals during the COVID-19 pandemic.Method: a cross-sectional and observational study, conducted with Nursing professionals through an electronic form in the five Brazilian regions. An instrument with general information was used, as well as the Patient Health Questionnaire-9 to identify depression symptoms. The Student’s t hypothesis and Analysis of Variance tests were adopted.Results: the participants were 3,249 professionals. Of these, 2,092 (64.4%) did not present depression symptoms or presented minimal symptoms; 603 (18.6%) presented moderate symptoms; 330 (10.2%) had moderate to severe symptoms; and 224 (6.9%), severe symptoms. Women, workers from the North region, young adults, single and with an income of up to four minimum wages presented higher depression scores (p<0.05).Conclusion: the Nursing professionals did not present depression symptoms, or presented mild symptoms of the disease. Variables such as gender, age group, marital status, region of the country, having contact with people infected by COVID-19, and not using masks presented significant differences with depression symptoms.Objetivo: identificar síntomas de depresión en profesionales de Enfermería durante la pandemia de COVID-19. Método: estudio transversal y observacional, realizado con profesionales de Enfermería por medio de un formulario electrónico, en las cinco regiones de Brasil. Se utilizó un instrumento con información general y el Patient Health Questionnaire-9 para identificar síntomas de depresión. Se adoptaron las pruebas de hipótesis t de Student y Análisis de Variancia. Resultados: participaron 3249 profesionales. De ellos, 2092 (64,4%) no presentaron síntomas de depresión o tuvieron síntomas mínimos, 603 (18,6%) moderados, 330 (10,2%) de moderados a graves y 224 (6,9%) síntomas graves. Las mujeres, los trabajadores de la región Norte, los adultos jóvenes, las personas solteras y con un ingreso de hasta cuatrosalarios presentaron puntajes de depresión más elevados (p<0,05). Conclusión: los profesionales de Enfermería no presentaron síntomas de depresión o tuvieron síntomas leves. Variables como el sexo, el grupo etario, el estado civil, la región del país, tener contacto con personas infectadas por COVID-19 y no usar máscaras evidenciaron diferencias significativas con los síntomas de depresión

FOLIAR COMPOSITION OF Eucalyptus urograndis IN TWO SITES FERTILIZED WITH N, P AND K IN SOUTHERN BRAZIL

The leaf composition has been used as a tool in establishing the nutritional diagnosis and evaluation of the effectiveness of the use of nutrients. This knowledge is still scarce in subtropical conditions of cultivation. In this sense, the study aims to evaluate the effect of N, P and K fertilization on leaf concentration of Eucalyptus urograndis. The experiments were installed in the Oxisols of the sandy-loam texture, in the counties of Jaguariaíva and Ventania, state of Paraná. In the N and P experiments, the following doses: 0; 30; 60; 120 and 240 kg ha-1 of N and P2O5; and 0; 35; 70; 140 and 280 kg ha-1 K2O, split in four applications: during planting and side dressing at 3, 9 and 12 months of age. The response was evaluated according to the nutritional status of the plants at 12, 24 and 36 months, by foliar concentration. It also evaluated levels of K deficiency plant through visual and chemical analysis. The application of N and P did not result in alteration in the leaf contents of the same in all evaluated periods and in the two regions, with a slight decrease in the concentrations with time. A different effect occurred with K at the Jaguariaíva site, which altered its foliar concentration at the 24th and 36th month, and increased foliar concentrations with time. These effects demonstrate that foliar diagnosis, through foliar concentrations, may present limitations of interpretation and should not be evaluated separately for fertilization recommendation

Treatment and characterization of biomass of soybean and rice hulls using ionic liquids for the liberation of fermentable sugars

We investigated the changes in the physical structure of cellulose recovered from soybean and rice hulls treated with the ionic liquids 1-butyl-3-methylimidazolium chloride ([bmim][Cl]) and 1-butyl-3-methylimidazolium acetate ([bmim][Ac]). The characterization was carried out by a combination of thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). Regenerated cellulose from soybean hull showed loss of crystallinity and high structural disruption caused by both ionic liquid treatments as compared to the untreated material. In contrast, rice hull presented only a small structural disruption when treated with [bmim][Ac] and was practically unaffected by [bmim][Cl], showing that this biomass residue is recalcitrance towards physico-chemical treatments, possibly as a consequence of its high composition content in silica. These results suggest the use of soybean hull as a substrate to be treated with ionic liquids in the preparation of lignocellulosic hydrolysates to be used in second-generation ethanol production, whereas other methods should be considered to treat rice hull biomass

In Vitro Hepatotoxic and Neurotoxic Effects of Titanium and Cerium Dioxide Nanoparticles, Arsenic and Mercury Co-Exposure

This article belongs to the Special Issue Toxicity of Nanomaterials and Legacy Contaminants: Risks to the Environment and Human Health.Considering the increasing emergence of new contaminants, such as nanomaterials, mixing with legacy contaminants, including metal(loid)s, it becomes imperative to understand the toxic profile resulting from these interactions. This work aimed at assessing and comparing the individual and combined hepatotoxic and neurotoxic potential of titanium dioxide nanoparticles (TiO2NPs 0.75-75 mg/L), cerium oxide nanoparticles (CeO2NPs 0.075-10 μg/L), arsenic (As 0.01-2.5 mg/L), and mercury (Hg 0.5-100 mg/L) on human hepatoma (HepG2) and neuroblastoma (SH-SY5Y) cells. Viability was assessed through WST-1 (24 h) and clonogenic (7 days) assays and it was affected in a dose-, time- and cell-dependent manner. Higher concentrations caused greater toxicity, while prolonged exposure caused inhibition of cell proliferation, even at low concentrations, for both cell lines. Cell cycle progression, explored by flow cytometry 24 h post-exposure, revealed that TiO2NPs, As and Hg but not CeO2NPs, changed the profiles of SH-SY5Y and HepG2 cells in a dose-dependent manner, and that the cell cycle was, overall, more affected by exposure to mixtures. Exposure to binary mixtures revealed either potentiation or antagonistic effects depending on the composition, cell type and time of exposure. These findings prove that joint toxicity of contaminants cannot be disregarded and must be further explored.This research was funded by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI), and by Portuguese funds through FCT—Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-029651. This work was also financed by national funds through FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of projects UIDB/04750/2020 and LA/P/0064/2020. This work was also developed within the scope of the project CICECO- Aveiro Institute of Materials, UIDB/50011/2020, UIDP/50011/2020 & LA/P/0006/2020, financed by national funds through the FCT/MEC (PIDDAC). A.T. Reis thanks the financial support of FCT through individual Grant SFRH/BPD/122112/2016. C.B. Lopes and A.C. Estrada acknowledge their research position funded by national funds (OE), through FCT—Fundação para a Ciência e a Tecnologia, I.P., in the scope of the framework contract foreseen in the numbers 4, 5 and 6 of the article 23, of the Decree-Law 57/2016, of August 29, changed by Law 57/2017, of July 19.info:eu-repo/semantics/publishedVersio

Participação comunitária na educação escolar quilombola: a Carta de Anuência no quilombo João Surá (PR)

ABSTRACT. Quilombola School Education arises from the struggle of quilombola populations for the right to a school education that corresponds to their particularities, which is understood as a school education that comes in addition to the quilombola education, already carried out in the daily life of community life. Thus, community participation in Quilombola School Education is essential for its realization. The João Surá quilombo, located in Paraná, has a Quilombola State School, in which since 2011 the Letter of Consent has been adopted as an instrument through which the community can appoint the civil servants to the school. The study here presented aims to analyze the adoption of the Letter of Consent at the aforesaid school, specially by taking the community participation in consideration. The research took place between 2018 and 2020, based on the action research methodology. The results indicate that the Letter of Consent has contributed to a process of greater community participation in school education, where the community appropriates from and signifies the instrument (i.e. the Letter of Consent), pointing out new possibilities for other communities.ABSTRACT. Quilombola School Education arises from the struggle of quilombola populations for the right to a school education that corresponds to their particularities, which is understood as a school education that comes in addition to the quilombola education, already carried out in the daily life of community life. Thus, community participation in Quilombola School Education is essential for its realization. The João Surá quilombo, located in Paraná, has a Quilombola State School, in which since 2011 the Letter of Consent has been adopted as an instrument through which the community can appoint the civil servants to the school. The study here presented aims to analyze the adoption of the Letter of Consent at the aforesaid school, specially by taking the community participation in consideration. The research took place between 2018 and 2020, based on the action research methodology. The results indicate that the Letter of Consent has contributed to a process of greater community participation in school education, where the community appropriates from and signifies the instrument (i.e. the Letter of Consent), pointing out new possibilities for other communities. &nbsp; Keywords: Quilombo João Surá (PR), CEQ Diogo Ramos, Community Participation, Letter of Consent.RESUMEN. La Educación Escolar Quilombola nace de la lucha de las poblaciones quilombolas por el derecho a una educación escolar que corresponda a sus especificidades, entendida como una educación escolar que se suma a la educación quilombola ya realizada en la vida diaria de vida comunitaria. Así, la participación comunitaria en la Educación Escolar Quilombola es fundamental para su realización. El quilombo João Surá, ubicado en Paraná, cuenta con un Colegio Estatal Quilombola, donde, desde 2011, se adopto la Carta de Consentimiento como un instrumento a través del cual la comunidad elige a los servidores que trabajarán en la escuela. El estudio aquí presentado tuvo como objetivo analizar la adopción de la Carta de Consentimiento en la mencionada escuela desde la perspectiva de la participación comunitaria. La investigación se realizó entre 2018 y 2020, basado en la metodología de investigación acción, donde, además de encuestas bibliográficas y documentales sobre el tema, se realizaron entrevistas con líderes comunitarios y con empleados de esa escuela. Los resultados indican que la Carta de Consentimiento ha contribuido a un proceso de mayor participación comunitaria en la educación escolar, donde la comunidad se apropia y significa el instrumento, señalando posibilidades para otras comunidades. &nbsp; Palabras clave: Quilombo João Surá (PR), CEQ Diogo Ramos, participación comunitaria, Carta de Consentimiento.ABSTRACT. Quilombola School Education arises from the struggle of quilombola populations for the right to a school education that corresponds to their particularities, which is understood as a school education that comes in addition to the quilombola education, already carried out in the daily life of community life. Thus, community participation in Quilombola School Education is essential for its realization. The João Surá quilombo, located in Paraná, has a Quilombola State School, in which since 2011 the Letter of Consent has been adopted as an instrument through which the community can appoint the civil servants to the school. The study here presented aims to analyze the adoption of the Letter of Consent at the aforesaid school, specially by taking the community participation in consideration. The research took place between 2018 and 2020, based on the action research methodology. The results indicate that the Letter of Consent has contributed to a process of greater community participation in school education, where the community appropriates from and signifies the instrument (i.e. the Letter of Consent), pointing out new possibilities for other communities.A Educação Escolar Quilombola nasce da luta das populações quilombolas pelo direito à uma educação escolar que corresponda às suas especificidades, sendo esta compreendida como uma educação escolar que vem a somar com a educação quilombola já realizada no cotidiano da vida comunitária. Desse modo, a participação comunitária na Educação Escolar Quilombola é fundamental para a sua realização. O quilombo João Surá, localizado no Paraná, conta com um Colégio Estadual Quilombola, onde, desde 2011, tem sido adotado a Carta de Anuência como um instrumento por meio do qual a comunidade escolhe os servidores que atuarão na escola. O estudo aqui apresentado teve como objetivo analisar a adoção da Carta de Anuência na escola mencionada sob a perspectiva da participação comunitária. A pesquisa foi realizada entre 2018 a 2020, tendo por base a metodologia de pesquisa-ação. Os resultados indicaram que a Carta de Anuência tem contribuído para um processo de maior participação comunitária na educação escolar, onde a comunidade se apropria e significa o instrumento, apontando possibilidades para outras comunidades. Palavras-chave: Quilombo João Surá (PR), CEQ Diogo Ramos, participação comunitária, Carta de Anuência. &nbsp; Community participation in quilombola school education: the Letter of Consent in the quilombo João Surá (PR) ABSTRACT. Quilombola School Education arises from the struggle of quilombola populations for the right to a school education that corresponds to their particularities, which is understood as a school education that comes in addition to the quilombola education, already carried out in the daily life of community life. Thus, community participation in Quilombola School Education is essential for its realization. The João Surá quilombo, located in Paraná, has a Quilombola State School, in which since 2011 the Letter of Consent has been adopted as an instrument through which the community can appoint the civil servants to the school. The study here presented aims to analyze the adoption of the Letter of Consent at the aforesaid school, specially by taking the community participation in consideration. The research took place between 2018 and 2020, based on the action research methodology. The results indicate that the Letter of Consent has contributed to a process of greater community participation in school education, where the community appropriates from and signifies the instrument (i.e. the Letter of Consent), pointing out new possibilities for other communities. Keywords: Quilombo João Surá (PR), CEQ Diogo Ramos, Community Participation, Letter of Consent. &nbsp; Participación comunitaria en la educación escolar quilombola: la carta de consentimiento en el quilombo João Surá (PR) RESUMEN. La Educación Escolar Quilombola nace de la lucha de las poblaciones quilombolas por el derecho a una educación escolar que corresponda a sus especificidades, entendida como una educación escolar que se suma a la educación quilombola ya realizada en la vida diaria de vida comunitaria. Así, la participación comunitaria en la Educación Escolar Quilombola es fundamental para su realización. El quilombo João Surá, ubicado en Paraná, cuenta con un Colegio Estatal Quilombola, donde, desde 2011, se adopto la Carta de Consentimiento como un instrumento a través del cual la comunidad elige a los servidores que trabajarán en la escuela. El estudio aquí presentado tuvo como objetivo analizar la adopción de la Carta de Consentimiento en la mencionada escuela desde la perspectiva de la participación comunitaria. La investigación se realizó entre 2018 y 2020, basado en la metodología de investigación acción, donde, además de encuestas bibliográficas y documentales sobre el tema, se realizaron entrevistas con líderes comunitarios y con empleados de esa escuela. Los resultados indican que la Carta de Consentimiento ha contribuido a un proceso de mayor participación comunitaria en la educación escolar, donde la comunidad se apropia y significa el instrumento, señalando posibilidades para otras comunidades. Palabras clave: Quilombo João Surá (PR), CEQ Diogo Ramos, participación comunitaria, Carta de Consentimiento. Palavras-chave: Quilombo João Surá (PR), CEQ Diogo Ramos, participação comunitária, Carta de Anuência

Avaliação de Impactos Ambientais na Rodovia mg - 010: Estudo de Caso no Vetor Norte de Belo Horizonte (MG)

O presente trabalho tem por objetivo avaliar os impactos ambientais dna Rodovia MG – 010 no trecho entre Hospital Risoleta Neves até o Viaduto Avenida Senhor do Bonfim (início da MG – 424), localizada no município de Belo Horizonte (MG), no período de 1989 e 2010. Para dar base aos estudos utilizaram-se os softawares Global Mapper e ArcGis para confecção dos mapas temáticos da área, e, ainda, registro fotográfico in loco dos principais impactos as margens da rodovia. Os resultados demonstraram que em um período de 20 anos a MG – 010 sofreu alterações em todos os compartimentos ambientais: físico (surgimento de ravinas e voçorocas), biótico (introdução de espécies exóticas e aumento das áreas de mata) e socioeconômico (aumento da circulação de veículos, atração de comércios e serviços e alargamento das autovias). Dentre os fatores que contribuíram para o desencadeamento deste processo está a expansão urbana-industrial direcionada ao Vetor Norte de Belo Horizonte

Chemometric approaches to evaluate the substitution of synthetic food dyes by natural compounds: The case of nanoencapsulated curcumin, spirulina, and hibiscus extracts

Finding natural food coloring options from plant-based sources to substitute artificial dyes is a challenging task because natural dyes often present low water-solubility, not very vibrant hues, and instability due to interactions with food ingredients. Chemometric approaches can be used to evaluate color differences and patterns resulting from natural and synthetic dyes when applied to food systems. Here, the Mixture Design and the Principal Component Analysis (PCA) were applied to evaluate the substitution of the following artificial food dyes: yolk yellow, apricot yellow, strawberry red, and tartrazine by natural dyes (water-soluble curcumin, yellow shade; Hibiscus sabdariffa extract, red shade; Spirulina platensis extract, blue/green shade), in three food simulated systems (phosphate buffer, pH 6.9; yogurt, pH 4.0 and citrate buffer, pH 3.0). The color parameters L*, a*, b*, C* and °h were determined and color difference (ΔE*) with artificial dyes resulted in 11 empirical models. PCA yielded a clear map for the identification of the closely matches natural/artificial dyes for the food simulated systems in three subregions. Furthermore, the antioxidant capacity of the natural dyes was determined by OxHLIA and TBARS. It was possible to make an assessment guide that may be useful for other food systems and dyes.Authors thank to CNPq (Chamada Universal– MCTI/CNPq Nº 28/2018, Process 421541/2018–0) and Fundação Araucária (convênio 039/2019) for the financial support. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. Authors thank to Central Analítica Multiusuário da UTFPR Campo Mourão (CAMulti-CM) by the color analysis. Authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES to the CIMO (UIDB/00690/2020). L. Barros and C. Pereira thank the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts.info:eu-repo/semantics/publishedVersio