2,590 research outputs found
AutomatizaciĂłn sistema control de acceso con lectores RFID
En este proyecto se tratarĂĄ de explicar los pasos necesarios para implementar un
sistema de control de accesos controlado a través de un lector RFID, el cual serå el
encargado de validar el acceso al usuario que intente acceder al espacio controlado y a la vez controlarĂĄ el resto de elementos del sistema.This Project describes the actions needed to install a controlled access system, using
a RFID coupler. RFID coupler is responsible of tag validation and control access system.IngenierĂa TĂ©cnica en ElectrĂłnic
Automatic identification of informative regions with epigenomic changes associated to hematopoiesis
Hematopoiesis is one of the best characterized biological systems but the connection between chromatin changes and lineage differentiation is not yet well understood. We have developed a bioinformatic workflow to generate a chromatin space that allows to classify 42 human healthy blood epigenomes from the BLUEPRINT, NIH ROADMAP and ENCODE consortia by their cell type. This approach let us to distinguish different cells types based on their epigenomic profiles, thus recapitulating important aspects of human hematopoiesis. The analysis of the orthogonal dimension of the chromatin space identify 32,662 chromatin determinant regions (CDRs), genomic regions with different epigenetic characteristics between the cell types. Functional analysis revealed that these regions are linked with cell identities. The inclusion of leukemia epigenomes in the healthy hematological chromatin sample space gives us insights on the healthy cell types that are more epigenetically similar to the disease samples. Further analysis of tumoral epigenetic alterations in hematopoietic CDRs points to sets of genes that are tightly regulated in leukemic transformations and commonly mutated in other tumors. Our method provides an analytical approach to study the relationship between epigenomic changes and cell lineage differentiation. Method availability: https://github.com/david-juan/ChromDet.European Unionâs Seventh Framework Programme [FP7/2007â2013, 282510 (BLUEPRINT)]; Spanish Ministry
of Economy, Industry and Competitiveness and European Regional Development Fund [Project Retos BFU2015â71241-R]. Funding for open access charge: Project Retos BFU2015â71241-R (to A.V.).Peer ReviewedPostprint (published version
Degenerations of Jordan Superalgebras
We describe degenerations of three-dimensional Jordan superalgebras over
. In particular, we describe all irreducible components in the
corresponding varieties.Comment: arXiv admin note: text overlap with arXiv:1611.0645
Fiber Coupled Transceiver with 6.5 THz Bandwidth for Terahertz Time-Domain Spectroscopy in Reflection Geometry
We present a fiber coupled transceiver head for terahertz (THz) time-domain reflection measurements. The monolithically integrated transceiver chip is based on iron (Fe) doped In0.53Ga0.47As (InGaAs:Fe) grown by molecular beam epitaxy. Due to its ultrashort electron lifetime and high mobility, InGaAs:Fe is very well suited as both THz emitter and receiver. A record THz bandwidth of 6.5 THz and a peak dynamic range of up to 75 dB are achieved. In addition, we present THz imaging in reflection geometry with a spatial resolution as good as 130 ”m. Hence, this THz transceiver is a promising device for industrial THz sensing applications
Host adaptive immunity deficiency in severe pandemic influenza
INTRODUCTION:
Pandemic A/H1N1/2009 influenza causes severe lower respiratory complications in rare cases. The association between host immune responses and clinical outcome in severe cases is unknown.
METHODS:
We utilized gene expression, cytokine profiles and generation of antibody responses following hospitalization in 19 critically ill patients with primary pandemic A/H1N1/2009 influenza pneumonia for identifying host immune responses associated with clinical outcome. Ingenuity pathway analysis 8.5 (IPA) (Ingenuity Systems, Redwood City, CA) was used to select, annotate and visualize genes by function and pathway (gene ontology). IPA analysis identified those canonical pathways differentially expressed (P < 0.05) between comparison groups. Hierarchical clustering of those genes differentially expressed between groups by IPA analysis was performed using BRB-Array Tools v.3.8.1.
RESULTS:
The majority of patients were characterized by the presence of comorbidities and the absence of immunosuppressive conditions. pH1N1 specific antibody production was observed around day 9 from disease onset and defined an early period of innate immune response and a late period of adaptive immune response to the virus. The most severe patients (n = 12) showed persistence of viral secretion. Seven of the most severe patients died. During the late phase, the most severe patient group had impaired expression of a number of genes participating in adaptive immune responses when compared to less severe patients. These genes were involved in antigen presentation, B-cell development, T-helper cell differentiation, CD28, granzyme B signaling, apoptosis and protein ubiquitination. Patients with the poorest outcomes were characterized by proinflammatory hypercytokinemia, along with elevated levels of immunosuppressory cytokines (interleukin (IL)-10 and IL-1ra) in serum.
CONCLUSIONS:
Our findings suggest an impaired development of adaptive immunity in the most severe cases of pandemic influenza, leading to an unremitting cycle of viral replication and innate cytokine-chemokine release. Interruption of this deleterious cycle may improve disease outcome.The study was scientifically sponsored by the Spanish Society for Critical Care Medicine (SEMICYUC). Funding: MICCIN-FIS/JCYL-IECSCYL-SACYL (Spain): Programa de Investigación Comisionada en Gripe, GR09/0021-EMER07/050- PI081236-RD07/0067. CIHR-NIH-Sardinia Recherché-LKSF Canada support DJK.S
Critical COPD respiratory illness is linked to increased transcriptomic activity of neutrophil proteases genes
BACKGROUND: Gene expression profiling (GEP) in cells obtained from peripheral blood has shown that this is a very useful approach for biomarker discovery and for studying molecular pathogenesis of prevalent diseases. While there is limited literature available on gene expression markers associated with Chronic Obstructive Pulmonary Disease (COPD), the transcriptomic picture associated with critical respiratory illness in this disease is not known at the present moment. FINDINGS: By using Agilent microarray chips, we have profiled gene expression signatures in the whole blood of 28 COPD patients hospitalized with different degrees of respiratory compromise.12 of them needed of admission to the ICU, whilst 16 were admitted to the Respiratory Medicine Service. GeneSpring GX 11.0 software was used for performing statistical comparisons of transcript levels between ICU and non-ICU patients. Ingenuity pathway analysis 8.5 (IPA) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to select, annotate and visualize genes by function and pathway (gene ontology). T-test showed evidence of 1501 genes differentially expressed between ICU and non-ICU patients. IPA and KEGG analysis of the most representative biological functions revealed that ICU patients had increased levels of neutrophil gene transcripts, being [cathepsin G (CTSG)], [elastase, neutrophil expressed (ELANE)], [proteinase 3 (PRTN3)], [myeloperoxidase (MPO)], [cathepsin D (CTSD)], [defensin, alpha 3, neutrophil-specific (DEFA3)], azurocidin 1 (AZU1)], and [bactericidal/permeability-increasing protein (BPI)] the most representative ones. Proteins codified by these genes form part of the azurophilic granules of neutrophils and are involved in both antimicrobial defence and tissue damage. This âneutrophil signatureâ was paralleled by the necessity of advanced respiratory and vital support, and the presence of bacterial infection. CONCLUSION: Study of transcriptomic signatures in blood suggests an essential role of neutrophil proteases in COPD patients with critical respiratory illness. Measurement and modulation of the expression of these genes could present an option for clinical monitoring and treatment of severe COPD exacerbations
Discovery and characterisation of detached M-dwarf eclipsing binaries in the WFCAM Transit Survey
We report the discovery of 16 detached M-dwarf eclipsing binaries with J<16
mag and provide a detailed characterisation of three of them, using
high-precision infrared light curves from the WFCAM Transit Survey (WTS). Such
systems provide the most accurate and model-independent method for measuring
the fundamental parameters of these poorly understood yet numerous stars, which
currently lack sufficient observations to precisely calibrate stellar evolution
models. We fully solve for the masses and radii of three of the systems,
finding orbital periods in the range 1.5<P<4.9 days, with masses spanning
0.35-0.50 Msun and radii between 0.38-0.50 Rsun, with uncertainties of
~3.5-6.4% in mass and ~2.7-5.5% in radius. Close-companions in short-period
binaries are expected to be tidally-locked into fast rotational velocities,
resulting in high levels of magnetic activity. This is predicted to inflate
their radii by inhibiting convective flow and increasing star spot coverage.
The radii of the WTS systems are inflated above model predictions by ~3-12%, in
agreement with the observed trend, despite an expected lower systematic
contribution from star spots signals at infrared wavelengths. We searched for
correlation between the orbital period and radius inflation by combining our
results with all existing M-dwarf radius measurements of comparable precision,
but we found no statistically significant evidence for a decrease in radius
inflation for longer period, less active systems. Radius inflation continues to
exists in non-synchronised systems indicating that the problem remains even for
very low activity M-dwarfs. Resolving this issue is vital not only for
understanding the most populous stars in the Universe, but also for
characterising their planetary companions, which hold the best prospects for
finding Earth-like planets in the traditional habitable zone.Comment: 30 pages, 14 figures, 16 tables, Accepted for publication in MNRA
Direct association between pharyngeal viral secretion and host cytokine response in severe pandemic influenza
<p>Abstract</p> <p>Background</p> <p>Severe disease caused by 2009 pandemic influenza A/H1N1virus is characterized by the presence of hypercytokinemia. The origin of the exacerbated cytokine response is unclear. As observed previously, uncontrolled influenza virus replication could strongly influence cytokine production. The objective of the present study was to evaluate the relationship between host cytokine responses and viral levels in pandemic influenza critically ill patients.</p> <p>Methods</p> <p>Twenty three patients admitted to the ICU with primary viral pneumonia were included in this study. A quantitative PCR based method targeting the M1 influenza gene was developed to quantify pharyngeal viral load. In addition, by using a multiplex based assay, we systematically evaluated host cytokine responses to the viral infection at admission to the ICU. Correlation studies between cytokine levels and viral load were done by calculating the Spearman correlation coefficient.</p> <p>Results</p> <p>Fifteen patients needed of intubation and ventilation, while eight did not need of mechanical ventilation during ICU hospitalization. Viral load in pharyngeal swabs was 300 fold higher in the group of patients with the worst respiratory condition at admission to the ICU. Pharyngeal viral load directly correlated with plasma levels of the pro-inflammatory cytokines IL-6, IL-12p70, IFN-Îł, the chemotactic factors MIP-1ÎČ, GM-CSF, the angiogenic mediator VEGF and also of the immuno-modulatory cytokine IL-1ra (p < 0.05). Correlation studies demonstrated also the existence of a significant positive association between the levels of these mediators, evidencing that they are simultaneously regulated in response to the virus.</p> <p>Conclusions</p> <p>Severe respiratory disease caused by the 2009 pandemic influenza virus is characterized by the existence of a direct association between viral replication and host cytokine response, revealing a potential pathogenic link with the severe disease caused by other influenza subtypes such as H5N1.</p
Intensity noise self-regulated solid-state laser at 1.5m using an ASHG based Buffer Reservoir
An absorption mechanism based on second-harmonic generation (ASHG) is
successfully implemented as a buffer reservoir in a solid-state Er,Yb:Glass
laser emitting at telecom wavelength. We show that a slight ASHG rate
conversion of 0.016% using a BBO crystal enables to cancel out the excess
intensity noise at the relaxation oscillation frequency, i.e. 35 dB reduction,
as well as to cancel the amplified spontaneous emission beating at the free
spectral range resonances of the laser lying in the GHz range.Comment: 6 pages, 5 figure
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