The Influence of oral environment on diet choices in goats: a focus on saliva protein composition

There is ample evidence that ruminants are capable of making choices between different foods that provide a more balanced diet that would be obtained by eating at random. In the particular case of goats, they occupy a diversity of habitats and different breeds present variability of feeding behaviors resultant from adaptations to the existent plant species. In their food search activity, individuals are faced with variable amounts of plant secondary metabolites (PSMs), which may present some toxic and anti-nutritional effects depending on the individual’s ability to deal with it. The oral cavity has a key role in the recognition and decision processes of ingestion or rejection. In this chapter we will first consider how goats identify foods and behave according to the food items available. Focus will be done on the importance of taste sense in this process and the information available on the main structures involved in taste detection and perception in goats will be reviewed. In a second section we will focus on the characteristics of goat’s saliva, particularly in terms of their protein composition, presenting results obtained by our research team

Studies of Copaifera luetzelburgii Harms in reproductive pharmacology: In vivo and in vitro approaches

The objective of this study was to evaluate the estrogenic and anti-estrogenic actions, as well as the reproductive and foetal toxicity, of the ethanol extract from Copaifera luetzelburgii (EEtOH-Cl). In the experiment of (anti) estrogenicity, nulliparous Wistar rats were treated for 3 days with EEtOH-Cl (125, 250 and 500 mg/kg); estradiol (E, 5 μg/kg); E + EEtOH-Cl; tamoxifen (T, 4mg/kg). This extract presented estrogenic activity by increasing the relative weight (%) of the uterus of rats treated at doses of 125, 250 and 500 mg/kg (0.267 ± 0.016*, 0.231 ± 0.014*, 0.242 ± 0.015*), and it showed anti-estrogenic activity when associated with estradiol (0.116 ± 0.006*, 0.103 ± 0.06*, 0.098 ± 0.05*), respectively. For assessment of toxicity in pregnancy, the animals were divided into two groups and treated daily with EEtOH-Cl. In the first group, the effect of the extract on the development of pregnancy from first to seventh day was observed, and in the second group, from 8 to 21 days, there was no change of these parameters or the viability of the progeny when the study assessed reproductive and foetal toxicity; however, there was shortening of pregnancy (125 mg/kg) without affecting the progeny. In the in vitro study, uterine strips of pregnant (P) and non-pregnant (NP) females were used. In both groups, half received EEtOH-Cl (vo) for 13 days (treated females - T), and the other half received EEtOH-Cl directly to the isolated organ bath system (untreated - NT). In vitro study on the uterus of pregnant animals pretreated with doses of 250 and 500 mg/kg showed that there was inhibition of KCl 80-induced phasic contractions (0.490 ± 0.110, 0.540 ± 0.092), respectively. Also, the contractions induced by oxytocin were inhibited at a dose of 500 mg/kg (0.380 ± 0.109). In non-pregnant, non-treated females, the extract at a concentration of 125 μg/mL (0.180 ± 0.062) also inhibited the contractions induced by oxytocin. Thus, EEtOH-Cl demonstrated estrogenic activity, but when combined with estradiol, it demonstrated anti-estrogenic activity. It did not induce toxicity in the progenitors or in the progeny, and it inhibited isometric contractions induced by oxytocin and KCl 80 mM in pregnant and non-pregnant rats.Keywords: Copaifera luetzelburgii, (anti-)estrogenicity, reproductive toxicity, phasic contractionsAfrican Journal of Biotechnology Vol. 12(24), pp. 3864-387

Relationship between power condition, agility, and speed performance among young roller hockey elite players

Purpose. The study was intended to describe the correlations between speed performance (11 m, 22 m, and 33 m) and agility test in skates with determinants of muscular power (squat jump, countermovement jump) in young Portuguese roller hockey athletes involved in a regional selection. Methods. A cross-sectional study was conducted among 10 male roller hockey players with mean (? SD) age of 14.20 (0.57) years, involved in the Portuguese national competition of under-15, making part of the final Oporto district selection of players to participate in inter-regional selections competition. Their mean (? SD) weight, height, body mass index, and sum of the skinfolds were 58.62 (8.78) kg, 165.72 (8.45) cm, 21.26 (1.52) kg/m2, 51.80 (14.91) mm, respectively. Furthermore, it strength was measured with squat jump and countermovement jump; sprinting time at 11 m, 22 m, and 33 m was determined, as well as time in an agility t-test, conducted in roller skating. Spearman correlation coefficients were calculated to test the associations. Results. Significant inverse correlations between vertical jumps, a practical method of power training monitoring, and linear velocity in skating (countermovement jump vs. speed, ?0.78) were found. Despite the increasing complexity of the agility test, a moderate inverse correlation with strength was observed, too (?0.48). Conclusions. Lower limbs explosive strength turned out a strong predictor of skating linear speed and agility among young roller hockey players, providing a simple evaluation tool of important determinants of performance.D915-7373-ED16 | Cesar LeaoN/

RELAT?RIO T?CNICO Projeto Integrador em Sistemas para Internet (PISI)

Relat?rio PISI - Gleidson Castelo Branco Magalh?es FilhoO Projeto Integrador em Sistemas para Internet pretende desenvolver a capacidade de aplica??o dos conceitos e teorias adquiridas durante o curso de forma integrada. Dessa maneira, o presente relat?rio ? consubstanciado em duas partes: a documenta??o de um produto implementado na forma de software (parte I) e as percep??es acad?micas dos autores sobre os variados t?picos e ?reas presentes no curso superior em Sistemas para Internet (parte II). O projeto de implementa??o se refere ao desenvolvimento de um software, p?gina web e/ou aplicativo h?brido com o objetivo de gerenciar campeonatos de skate. Por se tratar de projeto de implementa??o meramente com fins educacionais, a comercializa??o/venda do programa de computador oriundo desta implementa??o - transfer?ncia de tecnologia - n?o ? cab?vel neste trabalho. Por sua vez, as percep??es sobre a integra??o de t?picos e ?reas presentes no curso superior em Sistemas para Internet s?o descritas neste documento

Thermographic imaging in sports and exercise medicine: A Delphi study and consensus statement on the measurement of human skin temperature

This is an accepted manuscript of an article published by Elsevier in Journal of Thermal Biology on 18/07/2017, available online: https://doi.org/10.1016/j.jtherbio.2017.07.006 The accepted version of the publication may differ from the final published version.© 2017 Elsevier Ltd The importance of using infrared thermography (IRT) to assess skin temperature (tsk) is increasing in clinical settings. Recently, its use has been increasing in sports and exercise medicine; however, no consensus guideline exists to address the methods for collecting data in such situations. The aim of this study was to develop a checklist for the collection of tsk using IRT in sports and exercise medicine. We carried out a Delphi study to set a checklist based on consensus agreement from leading experts in the field. Panelists (n  =  24) representing the areas of sport science (n = 8; 33%), physiology (n = 7; 29%), physiotherapy (n = 3; 13%) and medicine (n = 6; 25%), from 13 different countries completed the Delphi process. An initial list of 16 points was proposed which was rated and commented on by panelists in three rounds of anonymous surveys following a standard Delphi procedure. The panel reached consensus on 15 items which encompassed the participants’ demographic information, camera/room or environment setup and recording/analysis of tsk using IRT. The results of the Delphi produced the checklist entitled “Thermographic Imaging in Sports and Exercise Medicine (TISEM)” which is a proposal to standardize the collection and analysis of tsk data using IRT. It is intended that the TISEM can also be applied to evaluate bias in thermographic studies and to guide practitioners in the use of this technique.Published versio

Identification of possible targets of the Aspergillus fumigatus CRZ1 homologue, CrzA

<p>Abstract</p> <p>Background</p> <p>Calcineurin, a serine/threonine-specific protein phosphatase, plays an important role in the control of cell morphology and virulence in fungi. Calcineurin regulates localization and activity of a transcription factor called CRZ1. Recently, we characterize <it>Aspergillus fumigatus CRZ1 </it>homologue, AfCrzA. Here, we investigate which pathways are influenced by <it>A. fumigatus </it>AfCrzA during a short pulse of calcium by comparatively determining the transcriptional profile of <it>A. fumigatus </it>wild type and <it>ΔAfcrzA </it>mutant strains.</p> <p>Results</p> <p>We were able to observe 3,622 genes modulated in at least one timepoint in the mutant when compared to the wild type strain (3,211 and 411 at 10 and 30 minutes, respectively). Decreased mRNA abundance in the <it>ΔcrzA </it>was seen for genes encoding calcium transporters, transcription factors and genes that could be directly or indirectly involved in calcium metabolism. Increased mRNA accumulation was observed for some genes encoding proteins involved in stress response. AfCrzA overexpression in <it>A. fumigatus </it>increases the expression of several of these genes. The deleted strain of one of these genes, AfRcnA, belonging to a class of endogenous calcineurin regulators, calcipressins, had more calcineurin activity after exposure to calcium and was less sensitive to menadione 30 μM, hydrogen peroxide 2.5 mM, EGTA 25 mM, and MnCl₂25 mM. We constructed deletion, overexpression, and GFP fusion protein for the closely related <it>A. nidulans </it>AnRcnA. GFP::RcnA was mostly detected along the germling, did not accumulate in the nuclei and its location is not affected by the cellular response to calcium chloride.</p> <p>Conclusion</p> <p>We have performed a transcriptional profiling analysis of the <it>A. fumigatus ΔAfcrzA </it>mutant strain exposed to calcium stress. This provided an excellent opportunity to identify genes and pathways that are under the influence of AfCrzA. AfRcnA, one of these selected genes, encodes a modulator of calcineurin activity. Concomitantly with <it>A. fumigatus AfrcnA </it>molecular analysis, we decided to exploit the conserved features of <it>A. nidulans </it>calcineurin system and investigated the <it>A. nidulans </it>AnRcnA homologue. <it>A. nidulans </it>AnRcnA mutation is suppressing CnaA mutation and it is responsible for modulating the calcineurin activity and mRNA accumulation of genes encoding calcium transporters.</p

The Samarco mud and corporate crime : a necessary reflection.

O presente estudo objetiva-se a lan?ar luzes sobre o tema de crime corporativo tal como defendido por Medeiros e Alcadipani (2013a). Os estudos organizacionais tendem a se debru?arem sobre o aspecto positivo das organiza??es, tratando o seu lado sombrio como algo excepcional. Diante disso, Medeiros e Alcadipani (2013a) buscaram explorar esse lado sombrio, especificamente os crimes corporativos. Destarte, busca-se lan?ar reflex?es acerca do evento da ?Lama da Samarco? que ocasionou na morte de dezenove pessoas, em fam?lias desabrigadas, preju?zos econ?micos, consequ?ncias ambientais e c?lculo de atingidos inestim?veis. As conclus?es no que se referem ? classifica??o do evento da ?Lama? como crime corporativo ainda ? prematura, por?m, defende-se a necessidade de tais reflex?es como forma de desvelar pr?ticas sombrias das organiza??es e, diante da atualidade desse acontecimento evidenciar como ado??o de tais pr?ticas ? presente na sociedade ao passo que pesquisas que abordem os aspectos negativos do management recebem pouco espa?o.This article refers to a theoretical essay that aims to contribute to critical organizational studies by shedding light on the subject of corporate crime. Organizations are often exploited in their positive aspects, treating their dark side as exceptional, and this ultimately favors the occurrence of corporate crimes. With this in mind, we seek to reflect on the event that occurred on November 5, 2015, in the municipality of Mariana (MG), known as the "Lama da Samarco", and the theory of corporate crimes, since this event caused death of nineteen people, in homeless families, economic losses, environmental consequences, and calculation of inestimable victims. The conclusions point to approximations and similarities between the "Samarco's Lama" event and the theory of corporate crimes, but the need for such reflections is defended as a way to observe shady practices of organizations, trigger critical thinking and promote transformative practices. Given the current relevance of this event, we seek to highlight the adoption of such practices present in society, reviewing the bias that research that addresses the negative aspects of management receive little academic space

Oxidation mechanisms occurring in wines

The present review aims to show the state of the art on the oxidation mechanisms occurring in wines, as well as the methods to monitor, classify and diagnose wine oxidation. Wine oxidation can be divided in enzymatic oxidation and non-enzymatic oxidation. Enzymatic oxidation almost entirely occurs in grape must and is largely correlated with the content of hydroxycinnamates, such as caffeoyltartaric acid and para-coumaroyltartaric acid, and flavan-3-ols. Non-enzymatic oxidation, also called chemical oxidation of wine, prevails in fermented wine and begin by the oxidation of polyphenols containing a catechol or a galloyl group. These phenolic. reactions, both enzymatic and non-enzymatic, result in by-products named quinones. However, in non-enzymatic oxidation, oxygen does not react directly with phenolic compounds. The limitation on the reactivity of triplet oxygen is overcome by the stepwise addition of a single electron, which can be provided by reduced transition metal ions, essentially iron(II) and copper(I). The sequential electron transfer leads to the formation of hydroperoxide radical (HOO center dot), hydrogen peroxide (H2O2), and hydroxyl radical (HO center dot). The later radical will oxidize almost any organic molecule found in wine and will react with the first species it encounters, depending on their concentration. Sulfur dioxide (SO2) and ascorbic acid, when added to wine, are able to reduce the quinones. Alternative options have been assessed for the prevention of oxidation during wine storage; nevertheless, these are not fully understood or commonly accepted. During aging, aldehydes are important intermediates in the chemical transformations occurring in wines, leading to color and flavor changes. In the same way, a range of off-flavors can be formed from wine oxidation. At low concentrations these flavors may add to the complexity of a wine, but as these increase they begin to detract from wine quality. In addition to the major chemical browning involving wine phenols, the main oxidation reactions occurring during grape juice heating or storage are caramelization and Maillard reaction, which are temperature dependent. Different methods have been proposed in the literature, addressing the complexity and multi-scale related with the oxidation process, to attempt the quantification of antioxidant activity in wines. These methods can be broadly divided in: i) methods based on chemical reactions and ii) methods based on the chemical-physical properties of antioxidants

Alfanet Deliverable 4.2 Second System Prototype

This deliverable describes the functionality included in the second prototype. In order to situate to the reader, first the system architecture is shortly described. Based on the identified modules, the second prototype functionality is explained, in addition to the functions already provided for the first prototype. This document is intended for internal audience (users and developers).IST 2001 3328