9 research outputs found

    Reliability of masticatory efficiency with beads and correlation with the muscle activity

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    TEMA: a avaliação da eficiência mastigatória pela análise colorimétrica com beads, pode ser um método promissor, mas não há relatos sobre a sua confiabilidade. OBJETIVO: investigar a confiabiabilidade das beads para teste de eficiência mastigatória e a correlação com a atividade eletromiográfica dos músculos masseter e temporal anterior. MÉTODO: participaram dezenove sujeitos adultos jovens, nove do gênero masculino e dez do feminino com idades entre dezoito e vinte-oito anos, com dentição completa, sem histórico de desordem temporomandibular, trauma, cirurgia na região de cabeça e pescoço, tratamento ortodôntico ou fonoaudiológico. O teste de eficiência mastigatória foi realizado com beads nas condições: mastigação habitual, mastigação unilateral direita e esquerda, com duração de 20 segundos. Simultaneamente, foi realizada a eletromiografia. A atividade em máxima intercuspidação habitual dos dentes também foi registrada. A quantidade de fucsina liberada após a mastigação foi medida usando o espectrofotômetro Beckman DU-7 UV-Visible (Beckman Inc., Palo Alto, CA, USA). RESULTADOS: houve alta confiabilidade do teste de eficiência mastigatória (r = 0,86, p < 0,01) e correlação significante com a atividade eletromiográfica (r = 0,76, p < 0,01). Também houve correlações positivas quando as provas foram analisadas separadamente. CONCLUSÃO: o teste de eficiência mastigatória realizado com beads mostrou-se um método confiável e correlacionado positivamente à atividade eletromiográfica dos músculos temporal anterior e músculos masseter.BACKGROUND: the use of the colorimetric method with beads to evaluate mastigatory efficiency may be promising, however no report is found about its reliability. AIM: to investigate the reliability of the beads to test masticatory efficiency and its correlation with the electromyographic activities of the anterior temporal and masseter muscles. METHODS: participants of this study were nineteen young adults, nine males and ten females, aged eighteen to twenty-eight years, with full dentition, Angle class I, with no history of temporomandibular disorder, neurological or cognitive deficit, previous or current tumors or traumas in the head and neck region, and orthodontic treatment or orofacial myofunctional therapy. The masticatory efficiency test was performed using beads, fuchsine-containing granules, in the folowing conditions: habitual chewing, right and left unilateral chewing, for 20 seconds. Electromyographic recordings were obtained simultaneously. Also, the maximal clenching was registered. The amount of fuchsin released upon chewing was measured using a Beckman DU-7 UV-Visible Spectrophotometer (Beckman Inc., Palo Alto, CA, USA). RESULTS: high reliability was observed for the masticatory efficiency test (r = 0.86, p < 0.01) and correlation with the electromyographic activities (r = 0.76, p < 0.01). Also, positive and significant correlations were observed when the conditions were separately analyzed. CONCLUSION: the masticatory efficiency test performed with beads proved to be a reliable method and positively correlated to the electromyographic activities of the anterior temporal and masseter muscles

    Analysis of masticatory function by means of clinical methods and \"differential Lissajous EMG figure\" in patients with temporomandibular disorder.

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    Objetivo: analisar a função mastigatória em pacientes com DTM articular, de longo prazo, por meio dos métodos clínicos de auto-avaliação da dificuldade para mastigar e avaliação miofuncional e do método instrumental differential Lissajous EMG figure, comparativamente a sujeitos controles, bem como verificar a relação entre as variáveis obtidas por esses métodos. Método: Participaram 55 pacientes com DTM articular, segundo o Research Diagnostic Criteria for TMD, eixo I (grupo DTM: 50 mulheres e 5 homens; média de idade 32, DP 11,34 anos) e 35 sujeitos saudáveis (grupo C: 31 mulheres e 4 homens; média de idade 29, DP 8,57 anos). As medidas empregadas foram: (a) auto-avaliação dos sinais e sintomas de DTM de acordo com o protocolo validado ProDTMmulti, (b) escala de dificuldade para mastigar; (c) avaliação miofuncional orofacial, de acordo com o protocolo validado AMIOFE, análise clínica da mastigação de acordo o protocolo AMIOFE-E, (d) eletromiografia de superfície (EMG) dos músculos temporais e masseteres (direito e esquerdo), registrada durante a realização de teste mastigatório padronizado com goma de mascar, analisada pelo Differential Lissajous EMG Figure (DL-EMG). A análise dos dados intervalares foi efetuada por estatística não paramétrica (Mann-Withney para amostras não pareadas). Aos dados contínuos, como da EMG, foi aplicada a análise de variância (ANOVA), com dois fatores de variação (grupo e lado) e o teste t-Student para amostras não pareadas, para o índice Simetria. As correlações entre as variáveis foram calculadas pelo coeficiente de correlação de Spearman. O nível de significância estabelecido foi P < 0,05. Resultados: Os sujeitos do grupo DTM, em comparação ao grupo C, indicaram maior severidade da sintomatologia (média 129,04, DP 82,04 x média 2,91, DP 4,75) (P < 0,000); maior dificuldade para mastigar (média 44,98, DP 17,84 x média 16,66, DP 10,04) (P < 0,000); pior condição miofuncional orofacial global (média 85,96, DP 5,61 x média 94,91, DP 4,74) (P < 0,000), pior escore quanto ao tipo mastigatório pelo AMIOFE-E (média 7,05, DP 3,03 x média 8,74, DP 1,84) (P < 0,01), e maior tempo despendido na mastigação do alimento (média 50,84, DP 11,50 x média 41,53, DP 11,02), (P < 0,001). Na EMG, o grupo DTM apresentou, na mastigação do lado direito, maior incoordenação que do lado esquerdo, bem como em comparação a mastigação à direita e à esquerda do grupo C, como verificado pelos valores médios do índice Fase direita (média 93,21, DP 103,05 x média 54,30, DP 73,79) e Fase esquerda (média 48,96, DP 36,36 x média 31,27, DP 18,63) (P < 0,01). A Simetria entre a mastigação dos lados direito e esquerdo foi menor no grupo DTM (média 45,91, DP 29,86 x média 68,52, DP 18,88) (P < 0,0001), o qual também utilizou maior atividade muscular total, representada pelo Impacto direito (média 1791,55, DP 1797,48 x média 1229,89, DP 573,39) e Impacto esquerdo (média 1762,98, DP 2097,67 x média 1159,16, DP 591,01) (P < 0,01) para a realização da função, bem como maior atividade percentual em relação à máxima contração voluntária, representada pelo Impacto% direita (média 89,61, DP 79,64 x média 66,20, DP 25,30) e Impacto% esquerda (média 89,09, DP 99,47 x média 65,82, DP 33,39) (P < 0,05). O escore de dificuldade para mastigar foi correlacionado de modo significante e negativo com a Simetria (r = - 0,25, P = 0,019) da DL-EMG e escore total do AMIOFE (r = - 0,49, P < 0,000). Conclusão: Pacientes com DTM articular relataram maior severidade da sintomatologia e maior dificuldade para mastigar. Clinicamente foi observado padrão mastigatório unilateral, o que pode explicar o menor índice de Simetria e o recrutamento aumentado dos músculos do lado de balanceio, que se refletiu na Fase (ângulo). A avaliação clínica e a EMG são métodos de diagnóstico complementares e úteis para o planejamento da reabilitação das funções motoras orofaciais.Objective: to analyze the masticatory function in patients with long lasting arthrogenous temporomandibular disorders (TMD), by clinical evaluation, self-assessment of chewing difficulty and \"differential Lissajous EMG figure\", compared to control subjects and to verify the relationship between the variables obtained by these methods. Method: 55 patients with arthrogenous TMD, according to Research Diagnostic Criteria for TMD, Axis I (TMD group: 50 women and 5 men, mean age 32, SD 11.34 years) and 35 healthy subjects (group C: 31 women and 4 men, mean age 29, SD 8.57 years). The employed measures were: (a) self-evaluation of the signs and symptoms of TMD according to the validated protocol ProDTMmulti, (b) scale of chewing difficulty and (c) orofacial myofunctional evaluation, according to the validated protocol OMES, clinical analysis of mastication, according the protocol OMES-E, (d) surface electromyography (EMG) of temporal and masseter muscles (right and left), recorded during the performance of a standard test with chewing gum, analyzed by the \"differential Lissajous EMG figure\". The analysis of the interval data was performed by nonparametric statistics (Mann-Whitney test for unpaired samples). To continuous data, such as EMG, was applied the analysis of variance (ANOVA) with two variation factors (group and side). and the Student-t test for unpaired samples, for the Symmetry index. The significance level was P < 0.05. Results: The TMD group, compared with C group, showed greater severity of symptoms (mean 129.04, SD 82.04 x mean 2.91, SD 4.75) (P < 0.000), greater chewing difficulty (mean 44.98, SD 17.84 x 16.66 mean, SD 10.04) (P < 0.000), worse overall orofacial myofunctional condition (mean 85.96, SD 5.61 x mean 94.91, SD 4.74) (P < 0.000), worse scores of the masticatory type, considered separately (mean 7.05, SD 3.03 x mean 8,74, SD 1.84), (P <0.01), and increased time spent to chewing the food (mean 50.84, SD 11.50 x mean 41.53, SD 11.02) (P <0.001). In the EMG tests, the TMD group presented in the right chewing, greater incoordination than the left side, as well as the right and left chewing compared to the C, as verified by the mean values of the right Phase index (mean 93.21, 103.05 x 54.30 SD mean, SD 73.79) and left Phase index (mean 48.96, SD 36.36 x mean 31.27, SD 18.63) (P <0.01). The Symmetry index between right and left chewing sides was lower in the TMD group (mean 45.91, SD 29.86 x mean 68.52, SD 18.88) (P <0.0001), which also used greater muscle total activity, ie, rigth Impact (mean 1791.55, SD 1797.48 x mean 1229.89, SD 573.39), left Impact (mean 1762.98, SD 2097.67 x mean 1159.16, SD 591.01) (P < 0,01) to perform the function as well as increased activity in relation to percentage maximum voluntary contraction, ie, right Impact% (mean 89.61, SD 79.64 x mean 66.20, SD 25.30) and left Impact% (mean 89.09, SD 99.47 x mean 65.82, SD 33.39) (P < 0.05). The score of chewing difficulty was found to be significantly and negative correlated with the symmetry index (SMI) (r = - 0.25, P = 0.019) and AMIOFE total score (r = - 0.49, P < 0.000). Conclusion: TMD patients reported greater severity of articular symptoms and greater difficulty chewing. Clinically, a unilateral chewing pattern was observed, which may explain the lower rate of symmetry and the increased recruitment of muscles in the balancing side, which was reflected in Phase (angle). The clinical evaluation and the EMG are complementary and useful diagnostic methods for planning the rehabilitation of orofacial motor functions

    Sinais e sintomas de desordem temporomandibular em mulheres e homens

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    RESUMO Introdução: Mulheres são mais susceptíveis às desordens temporomandibulares (DTM), contudo, estudos que tenham comparado os gêneros em amostras brasileiras são raros. Objetivo: Analisar a proporção de homens e mulheres, bem como a associação entre o gênero e as variáveis idade, duração do problema e sintomas de DTM em pacientes admitidos para tratamento em uma clínica universitária. Métodos: Foram coletados e analisados dados de entrevista e avaliação de mil protocolos de pacientes com diagnóstico de DTM, divididos em 2 grupos, masculino (n=177) e feminino (n=823). Foi realizada a análise exploratória a partir de tabelas de contingência e teste do χ2. Posteriormente, foi utilizado o modelo de regressão logística e calculadas as odds ratio brutas (OR) referentes às comparações avaliadas. Resultados: Na amostra prevaleceu o gênero feminino e as médias de idade e de duração da DTM foram semelhantes entre os grupos, predominando a faixa etária de adultos jovens (19 a 40 anos). Os valores de OR evidenciaram associação entre o gênero feminino e os sinais/sintomas dor na articulação temporomandibular (ATM), dor nos músculos faciais, pescoço e ombros, cefaleia, fadiga nos músculos mastigatórios, sintoma otológico e disfonia, tendo sido a chance das mulheres os apresentassem duas vezes maior do que os homens. Conclusão: Na amostra de pacientes brasileiros com DTM foi maior o número de mulheres e essas apresentaram maior prevalência de sintomas dolorosos, seguidos pelos otológicos e queixas de disfonia. A prevalência de ruído articular foi semelhante nos grupos estudados

    Characterisation of microbial attack on archaeological bone

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    As part of an EU funded project to investigate the factors influencing bone preservation in the archaeological record, more than 250 bones from 41 archaeological sites in five countries spanning four climatic regions were studied for diagenetic alteration. Sites were selected to cover a range of environmental conditions and archaeological contexts. Microscopic and physical (mercury intrusion porosimetry) analyses of these bones revealed that the majority (68%) had suffered microbial attack. Furthermore, significant differences were found between animal and human bone in both the state of preservation and the type of microbial attack present. These differences in preservation might result from differences in early taphonomy of the bones. © 2003 Elsevier Science Ltd. All rights reserved

    Núcleos de Ensino da Unesp: artigos 2009

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    Brazilian Flora 2020: Leveraging the power of a collaborative scientific network

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    International audienceThe shortage of reliable primary taxonomic data limits the description of biological taxa and the understanding of biodiversity patterns and processes, complicating biogeographical, ecological, and evolutionary studies. This deficit creates a significant taxonomic impediment to biodiversity research and conservation planning. The taxonomic impediment and the biodiversity crisis are widely recognized, highlighting the urgent need for reliable taxonomic data. Over the past decade, numerous countries worldwide have devoted considerable effort to Target 1 of the Global Strategy for Plant Conservation (GSPC), which called for the preparation of a working list of all known plant species by 2010 and an online world Flora by 2020. Brazil is a megadiverse country, home to more of the world's known plant species than any other country. Despite that, Flora Brasiliensis, concluded in 1906, was the last comprehensive treatment of the Brazilian flora. The lack of accurate estimates of the number of species of algae, fungi, and plants occurring in Brazil contributes to the prevailing taxonomic impediment and delays progress towards the GSPC targets. Over the past 12 years, a legion of taxonomists motivated to meet Target 1 of the GSPC, worked together to gather and integrate knowledge on the algal, plant, and fungal diversity of Brazil. Overall, a team of about 980 taxonomists joined efforts in a highly collaborative project that used cybertaxonomy to prepare an updated Flora of Brazil, showing the power of scientific collaboration to reach ambitious goals. This paper presents an overview of the Brazilian Flora 2020 and provides taxonomic and spatial updates on the algae, fungi, and plants found in one of the world's most biodiverse countries. We further identify collection gaps and summarize future goals that extend beyond 2020. Our results show that Brazil is home to 46,975 native species of algae, fungi, and plants, of which 19,669 are endemic to the country. The data compiled to date suggests that the Atlantic Rainforest might be the most diverse Brazilian domain for all plant groups except gymnosperms, which are most diverse in the Amazon. However, scientific knowledge of Brazilian diversity is still unequally distributed, with the Atlantic Rainforest and the Cerrado being the most intensively sampled and studied biomes in the country. In times of “scientific reductionism”, with botanical and mycological sciences suffering pervasive depreciation in recent decades, the first online Flora of Brazil 2020 significantly enhanced the quality and quantity of taxonomic data available for algae, fungi, and plants from Brazil. This project also made all the information freely available online, providing a firm foundation for future research and for the management, conservation, and sustainable use of the Brazilian funga and flora

    Núcleos de Ensino da Unesp: artigos 2008

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    Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

    Global variation in postoperative mortality and complications after cancer surgery: a multicentre, prospective cohort study in 82 countries

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    © 2021 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 licenseBackground: 80% of individuals with cancer will require a surgical procedure, yet little comparative data exist on early outcomes in low-income and middle-income countries (LMICs). We compared postoperative outcomes in breast, colorectal, and gastric cancer surgery in hospitals worldwide, focusing on the effect of disease stage and complications on postoperative mortality. Methods: This was a multicentre, international prospective cohort study of consecutive adult patients undergoing surgery for primary breast, colorectal, or gastric cancer requiring a skin incision done under general or neuraxial anaesthesia. The primary outcome was death or major complication within 30 days of surgery. Multilevel logistic regression determined relationships within three-level nested models of patients within hospitals and countries. Hospital-level infrastructure effects were explored with three-way mediation analyses. This study was registered with ClinicalTrials.gov, NCT03471494. Findings: Between April 1, 2018, and Jan 31, 2019, we enrolled 15 958 patients from 428 hospitals in 82 countries (high income 9106 patients, 31 countries; upper-middle income 2721 patients, 23 countries; or lower-middle income 4131 patients, 28 countries). Patients in LMICs presented with more advanced disease compared with patients in high-income countries. 30-day mortality was higher for gastric cancer in low-income or lower-middle-income countries (adjusted odds ratio 3·72, 95% CI 1·70–8·16) and for colorectal cancer in low-income or lower-middle-income countries (4·59, 2·39–8·80) and upper-middle-income countries (2·06, 1·11–3·83). No difference in 30-day mortality was seen in breast cancer. The proportion of patients who died after a major complication was greatest in low-income or lower-middle-income countries (6·15, 3·26–11·59) and upper-middle-income countries (3·89, 2·08–7·29). Postoperative death after complications was partly explained by patient factors (60%) and partly by hospital or country (40%). The absence of consistently available postoperative care facilities was associated with seven to 10 more deaths per 100 major complications in LMICs. Cancer stage alone explained little of the early variation in mortality or postoperative complications. Interpretation: Higher levels of mortality after cancer surgery in LMICs was not fully explained by later presentation of disease. The capacity to rescue patients from surgical complications is a tangible opportunity for meaningful intervention. Early death after cancer surgery might be reduced by policies focusing on strengthening perioperative care systems to detect and intervene in common complications. Funding: National Institute for Health Research Global Health Research Unit

    Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

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    © 2022 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 licenseBackground: Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide. Methods: A multimethods analysis was performed as part of the GlobalSurg 3 study—a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital. Findings: Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3·85 [95% CI 2·58–5·75]; p<0·0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63·0% vs 82·7%; OR 0·35 [0·23–0·53]; p<0·0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer. Interpretation: Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised. Funding: National Institute for Health and Care Research
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