Myocardium Metabolism in Physiological and Pathophysiological States: Implications of Epicardial Adipose Tissue and Potential Therapeutic Targets

The main energy substrate of adult cardiomyocytes for their contractility are the fatty acids. Its metabolism generates high ATP levels at the expense of high oxygen consumption in the mitochondria. Under low oxygen supply, they can get energy from other substrates, mainly glucose, lactate, ketone bodies, etc., but the mitochondrial dysfunction, in pathological conditions, reduces the oxidative metabolism. In consequence, fatty acids are stored into epicardial fat and its accumulation provokes inflammation, insulin resistance, and oxidative stress, which enhance the myocardium dysfunction. Some therapies focused on improvement the fatty acids entry into mitochondria have failed to demonstrate benefits on cardiovascular disorders. Oppositely, those therapies with effects on epicardial fat volume and inflammation might improve the oxidative metabolism of myocardium and might reduce the cardiovascular disease progression. This review aims at explain (a) the energy substrate adaptation of myocardium in physiological conditions, (b) the reduction of oxidative metabolism in pathological conditions and consequences on epicardial fat accumulation and insulin resistance, and (c) the reduction of cardiovascular outcomes after regulation by some therapies

High resolution 2-De-Based proteomics analysis of human atrial fibrilation

Comunicaciones a congreso

Estudios del secretoma y proteoma de grasa epicárdica han determinado niveles de proteínas implicadas en el transporte de lípidos más bajos en pacientes con cardiopatía isquémica

Comunicaciones a congreso

Proceedings of the 8th International Conference on Data Science, Technology and Applications (DATA 2019)

[Abstract] The aim of this work is to propose different statistical and machine learning methodologies for identifying anomalies and control the quality of energy efficiency and hygrothermal comfort in buildings. Companies focused on energy sector for buildings are interested on statistical and machine learning tools to automate the control of energy consumption and ensure quality of Heat Ventilation and Air Conditioning (HVAC) installations. Consequently, a methodology based on the application of the Local Correlation Integral (LOCI) anomaly detection technique has been proposed. In addition, the most critical variables for anomaly detection are identified by using ReliefF method. Once vectors of critical variables are obtained, multivariate and univariate control charts can be applied to control the quality of HVAC installations (consumption, thermal comfort). In order to test the proposed methodology, the companies involved in this project have provided the case study of a store of a clothing brand located in a shopping center in Panama. It is important to note that this is a controlled case study for which all the anomalies have been previously identified by maintenance personnel. Moreover, as an alternatively solution, in addition to machine learning and multivariate techniques, new nonparametric control charts for functional data based on data depth have been proposed and applied to curves of daily energy consumption in HVAC.Ministerio de Asuntos Económicos y Transformación Digital; MTM2014-52876-RMinisterio de Asuntos Económicos y Transformación Digital; MTM2017-82724-RXunta de Galicia; ED431C-2016-015Centro Singular de Investigación de Galicia; ED431G/01 2016-19Centro de Investigación en Tecnoloxías da Información e as Comunicacións da Universidade da Coruña; PC18/03Escuela Politécnica Nacional of Ecuador; PII-DM-002-201

Receptor for advanced glycation end-products expression in subcutaneous adipose tissue is related to coronary artery disease

OBJECTIVE: Obesity, a risk factor for coronary artery disease (CAD), is associated with inflammation and reactive oxygen species (ROS) production, while advanced glycation end-products, through their receptor (AGER or RAGE), play an important role on these processes. The aim of this study was to analyze the expression levels of RAGE, NADPH oxidase subunits, and catalase in adipose tissue in relation with CAD. DESIGN AND METHODS: Patients undergoing heart surgery were included in two groups: with and without CAD. Epicardial adipose tissue (EAT) and subcutaneous adipose tissue (SAT) biopsies were analyzed for gene expression by RT-quantitative PCR, immunohistochemistry, or western blot. RESULTS: RAGE mRNA and protein expression in SAT from patients with CAD was lower than in patients without CAD. However, there was no change in EAT from patients with or without CAD. P22-PHOX and RAGE gene expression were higher in EAT than in SAT, whereas catalase mRNA levels were lower. NADPH oxidase subunits and catalase mRNA expression were not influenced by CAD. Whereas NADPH oxidase-dependent oxidative response of SAT and EAT to lipid circulating levels could be different; glycemic levels were not related with the analyzed genes expression. CONCLUSIONS: This study demonstrates that RAGE expression in SAT, but not in EAT, is down-regulated in patients with CAD with respect to those without CAD. Although changes were not observed for NADPH oxidase subunits or catalase expression between CAD and non-CAD patients, a possible relationship between ROS production and RAGE expression in adipose tissues cannot be ruled out

CD5L, Macrophage Apoptosis Inhibitor, Was Identified in Epicardial Fat-Secretome and Regulated by Isoproterenol From Patients With Heart Failure

Objectives: Neurohormonal dysfunction, which can regulate epicardial fat activity, is one of the main promoters of atrial fibrillation (AF) in patients with heart failure (HF). Our aim was to study the epicardial fat mediators for AF in patients with HF and its catecholaminergic regulation. Methods: We have included 29 patients with HF who underwent cardiac surgery and were followed up for 5 years. Released proteins by epicardial adipose tissue (EAT) after isoproterenol treatment were identified by nano-high-performance liquid chromatography (HPLC) and triple time-of-flight (TOF) analysis. Common and differential identified proteins in groups of patients with AF before and after surgery were determined by the FunRich tool. Plasma and epicardial fat biopsy proteins were quantified by western blot. Results: Our results identified 17 common released proteins by EAT, after isoproterenol treatment, from HF patients who suffered AF or developed new-onset AF during follow-up. Mostly, they were involved on inflammatory response and extracellular matrix. One of them was CD5L, a macrophage apoptosis inhibitor. Its secretion by isoproterenol treatment was validated on western blot. The CD5L levels on epicardial fat were also higher in the group of male patients who present or develop AF (0.44 +/- 0.05 vs. 0.18 +/- 0.15; p < 0.016). However, there were no differences regarding plasma levels. Conclusion: Our results suggest the role of epicardial fat CD5L as a mediator of AF and its possible paracrine effect by catecholaminergic activity

Coulomb dissociation of O-16 into He-4 and C-12

We measured the Coulomb dissociation of O-16 into He-4 and C-12 within the FAIR Phase-0 program at GSI Helmholtzzentrum fur Schwerionenforschung Darmstadt, Germany. From this we will extract the photon dissociation cross section O-16(alpha,gamma)C-12, which is the time reversed reaction to C-12(alpha,gamma)O-16. With this indirect method, we aim to improve on the accuracy of the experimental data at lower energies than measured so far. The expected low cross section for the Coulomb dissociation reaction and close magnetic rigidity of beam and fragments demand a high precision measurement. Hence, new detector systems were built and radical changes to the (RB)-B-3 setup were necessary to cope with the high-intensity O-16 beam. All tracking detectors were designed to let the unreacted O-16 ions pass, while detecting the C-12 and He-4

Coulomb dissociation of 16O into 4He and 12C

We measured the Coulomb dissociation of 16O into 4He and 12C at the R3B setup in a first campaign within FAIR Phase 0 at GSI Helmholtzzentrum für Schwerionenforschung, Darmstadt. The goal was to improve the accuracy of the experimental data for the 12C(a,?)16O fusion reaction and to reach lower center-ofmass energies than measured so far. The experiment required beam intensities of 109 16O ions per second at an energy of 500 MeV/nucleon. The rare case of Coulomb breakup into 12C and 4He posed another challenge: The magnetic rigidities of the particles are so close because of the same mass-To-charge-number ratio A/Z = 2 for 16O, 12C and 4He. Hence, radical changes of the R3B setup were necessary. All detectors had slits to allow the passage of the unreacted 16O ions, while 4He and 12C would hit the detectors' active areas depending on the scattering angle and their relative energies. We developed and built detectors based on organic scintillators to track and identify the reaction products with sufficient precision

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Estudi, anàlisi i modelització de l'estructura del One World Trade Center de Minoru Yamasaki per mitjà de SAP2000

[ES] La finalidad del trabajo consiste en el análisis de la estructura del One World Trade Center, la torre norte de las conocidas Torres Gemelas, diseñadas por el arquitecto japonés-americano Minoru Yamasaki. La construcción de estas torres supuso una revolución en la construcción de los edificios en altura, convirtiéndose de 1971 a 1973 en uno de los edificios más altos del mundo. Se empleó el sistema de tubo estructural, que concentra el entramado de acero de alta resistencia en las fachadas del edificio. Este se convierte en fundamental para controlar la estabilidad lateral del edificio ante el factor que más afecta al diseño estructural de los rascacielos, el viento. La utilización de elementos prefabricados permitió una reducción importante del coste y tiempo de construcción, elevando así, una cantidad de espacio rentable superior a cualquier edificio contenido en el skyline de Manhattan hasta la fecha. Se analizará el arquitecto, contexto, obra y el sistema estructural empleado. El análisis estructural se realizará mediante el programa informático SAP 2000, realizando previamente un modelo con AutoCAD con los detalles y simplificaciones necesarias que se asemejen lo más posible a la realidad estructural. Mediante la herramienta SAP2000 se obtendrán los resultados del análisis del cual se realizarán las conclusiones del modelo y el comportamiento de la estructura del edificio.[EN] The objective of the work is to analyze the structure of the One World Trade Center, the north tower of the well-known Twin Towers, designed by the Japanese-American architect Minoru Yamasaki. The construction of these towers was a revolution in the construction of high-rise buildings, becoming from 1971 to 1973 in one of the tallest buildings in the world. It employed the structural tube system, which concentrates high-strength steel framing on the building's facades. This becomes essential to control the lateral stability of the building in the factor that most affects the structural design of skyscrapers, the wind. The use of prefabricated elements allowed for a significant reduction in cost and construction time, thus elevating an amount of rentable space superior to any building contained in the Manhattan skyline to date. The architect, context, construction site and the structural system employed will be analyzed. The structural analysis will be carried out using the computer program SAP 2000, previously making a model with AutoCAD with the necessary details and simplifications that are as close as possible to the structural reality. The SAP 2000 tool will be used to obtain the results of the analysis from which we will obtain the conclusions of the model and the behavior of the building's structure.Eiras Calvo, S. (2022). Estudio, análisis y modelización de la estructura del One World Trade Center de Minoru Yamasaki mediante SAP2000. Universitat Politècnica de València. http://hdl.handle.net/10251/19080