AVALIAÇÃO HEPÁTICA E RENAL EM EQUINOS COM PITIOSE TRATADOS COM IODETO DE POTÁSSIO, ATRAVÉS DA DETERMINAÇÃO DAS PROTEÍNAS, SUBSTÂNCIAS NITROGENADAS E ENZIMAS SÉRICAS

Nove equinos naturalmente infectados com Pythium insidiosum e oriundos de Campos dos Goytacazes-RJ foram tratados diariamente via oral com solução de iodeto de potássio (55mg/kg) por um período até duas semanas após a completa cura das lesões. Foram registrados dados sobre a involução das lesões e alterações nas taxas de proteínas, substâncias nitrogenadas e enzimas séricas dos animais. Estes apresentaram involução completa das lesões num período de 28 a 56 dias de tratamento, e o tempo de duração do tratamento foi diretamente proporcional ao tamanho das lesões. Além disto, não foram verificados parâmetros bioquímicos que permitam inferir que o tratamento tenha causado danos renais ou hepáticos aos animais. Desta forma, o iodeto de potássio, na dose de 55mg/kg de peso vivo, foi eficiente no tratamento da pitiose eqüina

Prevalence of Babesia spp. and Anaplasma marginale in cattle in the municipality of Palma, MG

ABSTRACT. Paula M.A.M., Oliveira F.C.R., Melo Jr O.A. & Frazão-Teixeira E. [Prevalence of Babesia spp. and Anaplasma marginale in cattle in the municipality of Palma, MG.] Prevalência de Babesia spp. e Anaplasma marginale em bovinos no município de Palma, MG. Revista Brasileira de Medicina Veterinária, 37(4): 359-365, 2015. Laboratório de Biologia Estrutural, Instituto Oswaldo Cruz/ Fiocruz, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ 21040-361, Brasil. E-mail: [email protected] We verified the prevalence of hemoparasites in 40 cattle with ages varying from one month to 12 years old, in two farms of the Municipality of Palma, Minas Gerais state, Brazil. Two blood smear samples were collected from each animal: one from the tail tip and another from the ear tip. The smears were fixed, stained and observed under 100X lighted microscope magnifying glass. Twenty- -seven out of 40 animals studied (67.5%) had at least one species of hemoparasite. Among these, 21 (52.5%) were infected with Babesia spp., 10 (25%) with Anaplasma marginale and four (10%) parasitized with both hemoparasites. The studied region is potentially enzootic for the detected parasites and there is high risk for clinical cases of tick-borne disease. Both anatomic points, tail and ear tips, are good spots for blood collection and smear confection for hemoparasite investigation

Compromised nutrition in gerbils infected by Cystoisospora felis detected through an animal performance analysis tool

The impact of Cystoisospora felis infection on the nutritional efficiency of gerbils was studied. The variables weight gain and feed intake were measured during four weeks in 28 laboratory gerbils, of which 14 were inoculated with 3.5 × 105 sporulated oocysts of C. felis and the remaining 14 were controls. The animals from both groups were weighted, killed, eviscerated and had their carcasses and tissues weighted and compared. A modern tool designed for measuring nutritional performance of farm animals was applied. The results showed compromised nutritional efficiency of the infected animals within the first week after infection. The consequences of these results are discussed here, including the potential impact of infection on farm animals performance.O impacto da infecção por Cystoisospora felis na eficiência nutricional de gerbis foi estudado. As variáveis ganho de peso e consumo de ração foram mensuradas durante quatro semanas em 28 gerbis de laboratório, dos quais 14 foram inoculados com 3,5 × 105 oocistos esporulados de C. felis e os 14 restantes serviram como controle. Os animais de ambos os grupos foram pesados, mortos, eviscerados e tiveram suas carcaças e tecidos pesados e comparados. Uma ferramenta moderna desenvolvida para mensurar o desempenho nutricional de animais de produção foi aplicada. Os resultados mostraram eficiência nutricional comprometida dos animais infectados na primeira semana após a infecção. As consequências destes resultados são discutidas aqui, incluindo o possível impacto de infecção no desempenho de animais de produção

RASTREABILIDADE DE PROPRIEDADES RURAIS PARA DIAGNÓSTICO DE FASCIOLOSE HEPÁTICA NO MUNICÍPIO DE CAMPOS DOS GOYTACAZES, RJ, BRASIL

No ano de 2001 foram identificadas pelo Serviço de Inspeção Estadual (S.I.E.) 41 propriedades rurais distribuídas em diversos municípios da região Norte Fluminense como sendo potenciais focos de fasciolose hepática. Dentre estas, 23 pertenciam ao município de Campos dos Goytacazes. Durante o período compreendido entre os meses de março de 2002 e dezembro de 2004 as propriedades localizadas neste município foram rastreadas para confirmação de casos, através da coleta de amostras fecais de bovinos para realização de exames pela técnica dos quatro tamises, bem como o recolhimento de moluscos do gênero Lymnaea para sua dissecação. Os resultados coproparasitológicos coincidiram, na maioria das vezes, com os diagnósticos anatomopatológicos realizados pelo S.I.E., pois indicaram a presença de animais parasitados em 17 (74%) das fazendas e, destas, oito (47%) pertenciam às localidades de Santa Maria, cinco (29%) de Morro do Coco e quatro (24%) de Santo Eduardo. A presença da espécie L. columella foi constatada somente em três propriedades rurais localizadas no distrito de Santa Maria; no entanto, nenhum molusco estava parasitado por formas larvais de Fasciola hepática; logo, a existência de casos autóctones não foi confirmada

Experimental infection with the Toxoplasma gondii ME-49 strain in the Brazilian BR-1 mini pig is a suitable animal model for human toxoplasmosis

Toxoplasma gondii causes toxoplasmosis, a worldwide disease. Experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. BR-1 mini pigs were intramuscularly infected with T. gondii with tachyzoites (RH strain) or orally infected with cysts (ME-49 strain). Haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. No alterations were observed in erythrocyte and platelet values; however, band neutrophils increased seven days after infection with ME-49. Serology of the mice inoculated with pig blood leucocytes revealed circulating ME-49 or RH strain tachyzoites in the pigs' peripheral blood at two and seven or nine days post-infection. The tachyzoites were also directly observed in blood smears from the infected pigs outside and inside leucocytes for longer periods. Alanine-aminotransferase was high at days 21 and 32 in the RH infected pigs. After 90 days, the pigs were euthanised and their tissue samples were processed and inoculated into mice. The mice serology revealed the presence of parasites in the hearts, ileums and mesenteric lymph nodes of the pigs. Additionally, cysts in the mice were only observed after pig heart tissue inoculation. The infected pigs presented similar human outcomes with relatively low pathogenicity and the BR-1 mini pig model infected with ME-49 is suitable to monitor experimental toxoplasmosis

The impact of surgical delay on resectability of colorectal cancer: An international prospective cohort study

AimThe SARS-CoV-2 pandemic has provided a unique opportunity to explore the impact of surgical delays on cancer resectability. This study aimed to compare resectability for colorectal cancer patients undergoing delayed versus non-delayed surgery.MethodsThis was an international prospective cohort study of consecutive colorectal cancer patients with a decision for curative surgery (January-April 2020). Surgical delay was defined as an operation taking place more than 4 weeks after treatment decision, in a patient who did not receive neoadjuvant therapy. A subgroup analysis explored the effects of delay in elective patients only. The impact of longer delays was explored in a sensitivity analysis. The primary outcome was complete resection, defined as curative resection with an R0 margin.ResultsOverall, 5453 patients from 304 hospitals in 47 countries were included, of whom 6.6% (358/5453) did not receive their planned operation. Of the 4304 operated patients without neoadjuvant therapy, 40.5% (1744/4304) were delayed beyond 4 weeks. Delayed patients were more likely to be older, men, more comorbid, have higher body mass index and have rectal cancer and early stage disease. Delayed patients had higher unadjusted rates of complete resection (93.7% vs. 91.9%, P = 0.032) and lower rates of emergency surgery (4.5% vs. 22.5%, P ConclusionOne in 15 colorectal cancer patients did not receive their planned operation during the first wave of COVID-19. Surgical delay did not appear to compromise resectability, raising the hypothesis that any reduction in long-term survival attributable to delays is likely to be due to micro-metastatic disease