13 research outputs found

    Application of Reverse Transcriptase-Polymerase Chain Reaction for Serotype Determination of Dengue Virus Strains Isolated in North Vietnam, 1991 to 1993

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    Reverse transcriptase-polymerase chain reaction (RT-PCR) was applied to determine serotypes of 20 dengue virus strains isolated in north Vietnam in the years of 1991 and 1993. There were 10 strains of dengue type 1 (D1), 9 strains of dengue type 2 (D2) and a single strain of dengue type 4 (D4), respectively. The results completely agreed with the serotypes determined by the immunofluorescent staining (IFA) of the infected cells using dengue type-specific monoclonal antibodies

    Low incidence of SARS-CoV-2, risk factors of mortality and the course of illness in the French national cohort of dialysis patients

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    Colorectal cancer and genetic polymorphisms of CYP1A1, GSTM1 and GSTT1: a case-control study in the Grampian region of Scotland

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    Cytochrome P-450 CYP1A1 is involved in the metabolism of polycyclic aromatic hydrocarbons (PAHs) that are derived from meat intake and tobacco smoking. Expression of the CYP1A1 gene is induced by compounds present in cruciferous vegetables. The glutathione S-transferases play a central role in the detoxification of carcinogens, including PAHs. We investigated the association between colorectal cancer and three variants (CYP1A1*2A, CYP1A1*2C, CYP1A1*4) of the CYP1A1 gene, and homozygosity for the null deletion of the GSTM1 and GSTT1 genes, and the joint effects of these genotypes and smoking, meat intake and intake of green leafy vegetables in a population-based study of 264 cases and 408 controls in Northeast Scotland. There was an inverse association with the CYP1A1*4 (m4) variant (OR 0.3, 95% CI 0.13-0.70). The OR for the CYP1A1*2C (m2) variant was 1.3 (95% CI 0.59-2.91), which is similar to a combined estimate for previous studies (OR 1.2, 95% CI 0.95-1.41). We observed no association with the CYP1A1*2A (m1) variant, or the GSTM1 and GSTT1 polymorphisms. Significant interactions between all 3 CYP1A1 variants and meat intake, and between the m1 and m2 variants and intake of green leafy vegetables, were observed. There was no evidence of interaction between CYP1A1 and smoking, and no evidence of interaction between the GSTM1 or GSTT1 polymorphisms and smoking, meat intake, green leafy vegetable intake, CYP1A1 variants or each other

    Fat and Cancer

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    Effect of double dose oseltamivir on clinical and virological outcomes in children and adults admitted to hospital with severe influenza: Double blind randomised controlled trial

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    10.1136/bmj.f3039BMJ (Online)3467911-BMJO

    Omega-3 Fatty Acids and Heart Disease

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