Organs on chip approach: A tool to evaluate cancer-immune cells interactions

In this paper we discuss the applicability of numerical descriptors and statistical physics concepts to characterize complex biological systems observed at microscopic level through organ on chip approach. To this end, we employ data collected on a micro uidic platform in which leukocytes can move through suitably built channels toward their target. Leukocyte behavior is recorded by standard time lapse imaging. In particular, we analyze three groups of human peripheral blood mononuclear cells (PBMC): heterozygous mutants (in which only one copy of the FPR1 gene is normal), homozygous mutants (in which both alleles encoding FPR1 are loss-of-function variants) and cells from ‘wild type’ donors (with normal expression of FPR1). We characterize the migration of these cells providing a quantitative con rmation of the essential role of FPR1 in cancer chemotherapy response. Indeed wild type PBMC perform biased random walks toward chemotherapy-treated cancer cells establishing persistent interactions with them. Conversely, heterozygous mutants present a weaker bias in their motion and homozygous mutants perform rather uncorrelated random walks, both failing to engage with their targets. We next focus on wild type cells and study the interactions of leukocytes with cancerous cells developing a novel heuristic procedure, inspired by Lyapunov stability in dynamical systems

From Petri Dishes to Organ on Chip Platform: The Increasing Importance of Machine Learning and Image Analysis

The increasing interest for microfluidic devices in medicine and biology has opened the way to new time-lapse microscopy era where the amount of images and their acquisition time will become crucial. In this optic, new data analysis algorithms have to be developed in order to extract novel features of cell behavior and cell–cell interactions. In this brief article, we emphasize the potential strength of a new paradigm arising in the integration of microfluidic devices (i.e., organ on chip), time-lapse microscopy analysis, and machine learning approaches. Some snapshots of previous case studies in the context of immunotherapy are included as proof of concepts of the proposed strategies while a visionary description concludes the work foreseeing future research and applicative scenarios

Presence of Mycobacterium bovis in slaughterhouses and risks for workers

An investigation was carried out to detect the presence of Mycobacterium bovis in slaughterhouses where intradermal tuberculin test positive cattle were slaughtered, and to evaluate the risk of contamination by M. bovis among exposed slaughterhouse workers. Swabs were taken from the carcasses of slaughtered animals showing autoptic signs of non-generalized forms of tuberculosis, thus authorized for free consumption. Swabs were also taken from the hands and clothes of the staff employed in the butchery production line. Environmental samplings were conducted on the slaughterhouse air using filters and air aspiration devices, and on water used to wash the carcasses after slaughter. Samples from the carcasses of healthy animals were also taken on a following slaughtering session. The swabs were analysed by means of Polymerase Chain Reaction for the detection of mycobacteria. M. bovis was detected on meats, on the hands of one worker, and in the washing water. The results obtained from this study confirm that workers are highly exposed to infection by zoonotic tuberculosis, and that cleaning procedures were ineffective in our setting

“How a good video can remove ‘barriers’ for a more inclusive science communication”

There is a burgeoning body of evidence suggesting that technology can enhance learning, and multiple studies have shown that videos represent a very effective tool in science engagement and education. In fact, video media can transform the complexities of science and nature into something more tangible and tractable (Dabylchuk et al., 2018). Documentaries can hence be powerful tools for learning, to raise awareness for important topics, and adding a scientific approach makes it possible to deepen knowledge about the world. According to Dale’s cone of experience (Fig. 1), people memorize 10% of what they read, 20% of what they hear, 30% of what they see and 50% of what they hear and see (Wiman and Mierhenry, 1969). These statistics seems to convey a very clear message: blind or visually impaired people are penalised in the learning compared to sighted ones. However, our experience in the area of inclusive science communication has led us to wonder: are we sure that sight is a fundamental means for learning? Or is it possible that sight is a predominant sense over others, and therefore it can even mislead or limit learning? To answer this question, we held an ad-hoc educational laboratory with the users of the “IstitutodeiCiechiOpereRiunite I. Florio – F. ed A. Salamone” of Palermo, with which we have established a convention on 29/01/2019. Specifically, we involved 20 users with different degrees of visual impairment, in the projection of three science videos, selected from those realised by the EDUlab divulgation group over the years. At the end of the video projection, users were asked to express their opinions and their personal interpretations of scientific issues, allowing us to understand what e how many information have been received and, above all, if sighted usersare really advantaged compared to the visually impaired ones

“Beaks and berries”: zoological laboratory (II) of the formative proposal of inclusive scientific divulgation

Searching a topic that help us about the implementation of an inclusive divulgation, aimed also at sensory disabilitiesusers, we have chosen birds as a key to connect different scientific issues (pollution, environmental changes, exploitation of resources) and create new knowledge in a heterogeneous auditorium, through the suggestions that the workshop lessons can infuse on the users, whether they are disabled or not. All this to bring them closer to thescientific contents to which they do not have easy access. In particular, the project aims to develop, deepen and consolidate, through communication and information, issues concerning the concept of biodiversity, in order to sensitize users to safeguard the species and habitats of our territory and make them as the main actors of scientific dissemination.Indeed, weestabilisheda convention between the “Istituto dei Ciechi Opere riunite I. Florio – F. ed A. Salamone” of Palermo and the “Consiglio Nazionale delle Ricerche - Istituto per lo studio degli impatti Antropici e Sostenibilità in ambiente marino” of Capo Granitola (TP). The main role of this convention is to bring disable people closer to these scientific subjects and make the contents of our laboratories as inclusive as possible.The project "The world of birds", developed from January to April 2019 is divided into three main themes, the second of which is the focus of this report: The bird alimentation

“For whom the Calandra tolls?”: zoological laboratory (III) of the formative proposal of inclusive scientific divulgation

Blind people, though not seeing the world, is nevertheless able to imagine it, activating mental processes different from those of the sighted. They can come in contact with the reality of the world and know it with appreciable effectiveness (Angeli F., 2012). It has been imagined that only the view was the capable sense of allowing the conception of space and its forms. Of course, it was the facts that showed, with increasing clarity, that the lack of sight does not prevent the mental construction of space. Although touch is generally regarded as the sight of the blind, it is important to understand how sight cannot be validly replaced by a single sense. To organize a good relationship with the surrounding reality, the person who does not see needs to activate the whole of their residual senses. In particular, the complementarity between touch and hearing makes us understand like the blind. The touch sense has a very small perceptive field and therefore proceeds bysuccession of spatial fragments, but also presents a very analytical capacity,refined and punctual. Hearing has a greatly extended perceptual field, whichallows the blind to have a broad overall spatial reference but offers insufficient information on the objects and on the particular characteristics of the surrounding space (Coppa M.M., 1997).We have therefore devised a playful-practical laboratory to allow a sensory disabilitypublic,with various degrees of blindness, to reach zoological and scientific contents and then re-connect to more general problems such as the numerous environmental emergencies of this decade. Thanks to a convention between the “IstitutodeiCiechiOpereriunite I. Florio – F. ed A. Salamone” of Palermo and the “Consiglio Nazionale delleRicerche - Istituto per lo studio degliimpattiAntropici e Sostenibilità in ambientemarino” of Capo Granitola (TP), we start with the project "The world of birds", developed from January to April 2019 and divided into three main themes. The third of which is the focus of this report: Nest and songs

“On the science’s wing”: zoological laboratory (I) of the formative proposal of inclusive scientific divulgation

The scientific divulgation work is increasingly like a facilitator task. In fact, in this work it is primary to transform the results and the notions of scientific research so that they became accessible to the public, understood as the largest possible number of people. However, this information flow turns out to be purely verbal, moreover with a language rich of visual references, that are inaccessible and incomprehensible for the user with sensory disabilities. Wehavethusestablished a convention between the “Istituto dei Ciechi Opere riunite I. Florio – F. ed A. Salamone” of Palermo and the “Consiglio Nazionale delle Ricerche - Istituto per lo studio degli impatti Antropici e Sostenibilità in ambiente marino” of Capo Granitola (TP). Wedeveloped a formative proposal that links the science world with sensory disabilities, proposing Birds as principal argument and the whole world connected to it. The birds appear as the key to connect to different scientific issues (pollution, environmental changes, exploitation of resources) to create new knowledge in the public, through the suggestions that the workshop lessons can give people, both disabled or not. All this in order to bring them closer to these subjects and make the contents of our laboratories as inclusive as possible. The project "The world of birds", developed from January to April 2019 is divided into three main themes, the first of which is the focus of this report: The bird plumage

Antimicrobial resistance among migrants in Europe: a systematic review and meta-analysis

BACKGROUND: Rates of antimicrobial resistance (AMR) are rising globally and there is concern that increased migration is contributing to the burden of antibiotic resistance in Europe. However, the effect of migration on the burden of AMR in Europe has not yet been comprehensively examined. Therefore, we did a systematic review and meta-analysis to identify and synthesise data for AMR carriage or infection in migrants to Europe to examine differences in patterns of AMR across migrant groups and in different settings. METHODS: For this systematic review and meta-analysis, we searched MEDLINE, Embase, PubMed, and Scopus with no language restrictions from Jan 1, 2000, to Jan 18, 2017, for primary data from observational studies reporting antibacterial resistance in common bacterial pathogens among migrants to 21 European Union-15 and European Economic Area countries. To be eligible for inclusion, studies had to report data on carriage or infection with laboratory-confirmed antibiotic-resistant organisms in migrant populations. We extracted data from eligible studies and assessed quality using piloted, standardised forms. We did not examine drug resistance in tuberculosis and excluded articles solely reporting on this parameter. We also excluded articles in which migrant status was determined by ethnicity, country of birth of participants' parents, or was not defined, and articles in which data were not disaggregated by migrant status. Outcomes were carriage of or infection with antibiotic-resistant organisms. We used random-effects models to calculate the pooled prevalence of each outcome. The study protocol is registered with PROSPERO, number CRD42016043681. FINDINGS: We identified 2274 articles, of which 23 observational studies reporting on antibiotic resistance in 2319 migrants were included. The pooled prevalence of any AMR carriage or AMR infection in migrants was 25·4% (95% CI 19·1-31·8; I2 =98%), including meticillin-resistant Staphylococcus aureus (7·8%, 4·8-10·7; I2 =92%) and antibiotic-resistant Gram-negative bacteria (27·2%, 17·6-36·8; I2 =94%). The pooled prevalence of any AMR carriage or infection was higher in refugees and asylum seekers (33·0%, 18·3-47·6; I2 =98%) than in other migrant groups (6·6%, 1·8-11·3; I2 =92%). The pooled prevalence of antibiotic-resistant organisms was slightly higher in high-migrant community settings (33·1%, 11·1-55·1; I2 =96%) than in migrants in hospitals (24·3%, 16·1-32·6; I2 =98%). We did not find evidence of high rates of transmission of AMR from migrant to host populations. INTERPRETATION: Migrants are exposed to conditions favouring the emergence of drug resistance during transit and in host countries in Europe. Increased antibiotic resistance among refugees and asylum seekers and in high-migrant community settings (such as refugee camps and detention facilities) highlights the need for improved living conditions, access to health care, and initiatives to facilitate detection of and appropriate high-quality treatment for antibiotic-resistant infections during transit and in host countries. Protocols for the prevention and control of infection and for antibiotic surveillance need to be integrated in all aspects of health care, which should be accessible for all migrant groups, and should target determinants of AMR before, during, and after migration. FUNDING: UK National Institute for Health Research Imperial Biomedical Research Centre, Imperial College Healthcare Charity, the Wellcome Trust, and UK National Institute for Health Research Health Protection Research Unit in Healthcare-associated Infections and Antimictobial Resistance at Imperial College London

Clinical Features, Cardiovascular Risk Profile, and Therapeutic Trajectories of Patients with Type 2 Diabetes Candidate for Oral Semaglutide Therapy in the Italian Specialist Care

Introduction: This study aimed to address therapeutic inertia in the management of type 2 diabetes (T2D) by investigating the potential of early treatment with oral semaglutide. Methods: A cross-sectional survey was conducted between October 2021 and April 2022 among specialists treating individuals with T2D. A scientific committee designed a data collection form covering demographics, cardiovascular risk, glucose control metrics, ongoing therapies, and physician judgments on treatment appropriateness. Participants completed anonymous patient questionnaires reflecting routine clinical encounters. The preferred therapeutic regimen for each patient was also identified. Results: The analysis was conducted on 4449 patients initiating oral semaglutide. The population had a relatively short disease duration (42%  60% of patients, and more often than sitagliptin or empagliflozin. Conclusion: The study supports the potential of early implementation of oral semaglutide as a strategy to overcome therapeutic inertia and enhance T2D management