Biogenic synthesis of gold nanoparticles using Sargassum tenerrimum and its evaluation of antibacterial activity against Escherichia coli and Salmonella typhi

1291-1297An important aspect of nanotechnology is the development of a reliable and ecofriendly method for the synthesis of nanomaterials. In the present study, Sargassum tenerrimum extract was used to synthesize gold nanoparticles (AuNPs) by the reduction of AuCl4− ions to Au0. The formation and morphology of the synthesized AuNPs were investigated using \UV–visible spectroscopy, Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, and field emission scanning electron microscopy with energy dispersive X-ray analysis. The nanoparticles synthesized at 60 °C were spherical in shape while few were also hexagonal in shape with the size ranging between 10 and 40 nm. The antibacterial activity of AuNPs was tested against Escherichia coli (ATCC 25922) and Salmonella typhi (ATCC 6539). On examination under an epifluorescence microscope, it was found to cause a significant amount of deterioration to the bacterial cells

In vitro anticancer activity of Sargassum sp. polysaccharides against MCF-7 cell lines

1267-1273Seaweed polysaccharides are compounds with promising chemopreventive and chemotherapeutic activities. In this study, polysaccharides isolated from Sargassum seaweeds were tested for their efficiency in cancer therapy. Polysaccharides were isolated, purified, and characterized using nuclear magnetic resonance (NMR); and Fourier transform infrared (FT-IR) spectroscopic and matrix-assisted laser desorption ionization mass spectrometric (MALDI-MS). It was found that the polysaccharides from Sargassum sp. branches are sulfated galactose–fucose disaccharides and sulfated galactose monosaccharides attached to the main chain through (1–4) linkages. Further, the polysaccharides were tested for cytotoxicity and anticancer activity against human breast adenocarcinoma cell line (MCF-7) using the Annexin-V/propidium iodide (PI) staining method

Green synthesis of well dispersed nanoparticles using leaf extract of medicinally useful adhatoda Vasica Nees

Development of reliable method for the green synthesis of gold nanoparticles (AuNPs) using medicinally valued Adhatoda vasica Nees has been studied here. The color change and the Surface Plasmon Resonance (SPR) confirmed the formation of AuNPs. The biosynthesized AuNPs were characterized using UV-visible Spectroscopy (UV-vis), Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDAX) and Transmission Electron Microscopy (TEM) analysis. The nanoparticles synthesized were predominantly monodisperse, stable spherical in nature with well-defined dimensions of size ranging from 22 to 47 nm. The crystalline nature of the synthesized particles was also evident by the X-ray diffraction analysis

Purification and Characterization of Gum-Derived Polysaccharides of Moringa oleifera and Azadirachta indica and Their Applications as Plant Stimulants and Bio-Pesticidal Agents

Plant gums are bio-organic substances that are derived from the barks of trees. They are biodegradable and non-adverse complex polysaccharides that have been gaining usage in recent years due to a number of advantages they contribute to various applications. In this study, gum was collected from Moringa oleifera and Azadirachta indica trees, then dried and powdered. Characterizations of gum polysaccharides were performed using TLC, GC-MS, NMR, etc., and sugar molecules such as glucose and xylose were found to be present. Effects of the gums on Abelmoschus esculentus growth were observed through root growth, shoot growth, and biomass content. The exposure of the seeds to the plant gums led to bio stimulation in the growth of the plants. Poor quality soil was exposed to the gum polysaccharide, where the polysaccharide was found to improve soil quality, which was observed through soil analysis and SEM analysis of soil porosity and structure. Furthermore, the plant gums were also found to have bio-pesticidal activity against mealybugs, which showed certain interstitial damage evident through histopathological analysis