Methicillin-Resistant Coagulase-Negative Staphylococci in the Community: High Homology of SCCmec IVa between Staphylococcus epidermidis and Major Clones of Methicillin-Resistant Staphylococcus aureus

Background. Data on community spread of methicillin-resistant coagulase-negative staphylococci (MR-CoNS) are scarce. We assessed their potential role as a reservoir of staphylococcal cassette chromosome mec (SCCmec) IVa, the leading SCCmec subtype in community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA). Methods. Nasal carriage of MR-CoNS was prospectively investigated in 291 adults at hospital admission. MR-CoNS were characterized by SCCmec typing, long-range polymerase chain reaction (PCR) for SCCmec IV, and multiple-locus variable-number tandem repeat analysis (MLVA) for Staphylococcus epidermidis (MRSE) strains. Three SCCmec IVa elements were fully sequenced. Results. The carriage rate of MR-CoNS was 19.2% (25.9% and 16.5% in patients with and patients without previous exposure to the health care system, respectively; P = .09). MR-CoNS strains (n=83, including 58 MRSE strains with highly heterogeneous MLVA patterns) carried SCCmec type IVa (n=9, all MRSE), other SCCmec IV subtypes (n=9, including 7 MRSE), other SCCmec types (n=15), and nontypeable SCCmec (n=50). Long-range PCR indicated structural homology between SCCmec IV in MRSE and that in MRSA. Complete sequences of SCCmec IVa from 3 MRSE strains were highly homologous to those available for CA-MRSA, including major clones USA300 and USA400. Conclusions. MR-CoNS are probably disseminated in the community, notably in subjects without previous exposure to the health care system. MRSE, the most prevalent species, may act as a reservoir of SCCmec IVa for CA-MRS

E-Biothon : Une plate-forme pour accélérer les recherches en biologie, santé et environnement

National audienceE-Biothon : Une plate-forme pour accélérer les recherches en biologie, santé et environnemen

Report of the Working Group on the Effects of Extraction of Marine Sediments on the Marine Ecosystem, WGEXT, 31 May-4 June 2010, Djurönäset, Sweden

ICES WGEXT Report 201

Report of the Working Group on the Effects of Extraction of Marine Sediments on the Marine Ecosystem, WGEXT, 11-15 April 2011, Delft, the Netherlands

ICES WGEXT Report 201

Grid'5000: a large scale and highly reconfigurable grid experimental testbed

Large scale distributed systems such as Grids are difficult to study from theoretical models and simulators only. Most Grids deployed at large scale are production plat-forms that are inappropriate research tools because of their limited reconfiguration, control and monitoring capa-bilities. In this paper, we present Grid’5000, a 5000 CPU nation-wide infrastructure for research in Grid computing. Grid’5000 is designed to provide a scientific tool for com-puter scientists similar to the large-scale instruments used by physicists, astronomers, and biologists. We describe the motivations, design considerations, architec-ture, control, and monitoring infrastructure of this experi-mental platform. We present configuration examples and performance results for the reconfiguration subsystem

Introduction to the French GEOTRACES North Atlantic transect (GA01): GEOVIDE cruise

© 2018 Author(s). The GEOVIDE cruise, a collaborative project within the framework of the international GEOTRACES programme, was conducted along the French-led section in the North Atlantic Ocean (Section GA01), between 15 May and 30 June 2014. In this special issue (https://www.biogeosciences.net/special-issue900.html), results from GEOVIDE, including physical oceanography and trace element and isotope cyclings, are presented among 18 articles. Here, the scientific context, project objectives, and scientific strategy of GEOVIDE are provided, along with an overview of the main results from the articles published in the special issue

The MUSE Hubble Ultra Deep Field Survey:II. Spectroscopic redshifts and comparisons to color selections of high-redshift galaxies

We have conducted a two-layered spectroscopic survey (1'x1' ultra deep and 3'x3' deep regions) in the Hubble Ultra Deep Field (HUDF) with the Multi Unit Spectroscopic Explorer (MUSE). The combination of a large field of view, high sensitivity, and wide wavelength coverage provides an order of magnitude improvement in spectroscopically confirmed redshifts in the HUDF; i.e., 1206 secure spectroscopic redshifts for HST continuum selected objects, which corresponds to 15% of the total (7904). The redshift distribution extends well beyond z>3 and to HST/F775W magnitudes as faint as ~30 mag (AB, 1-sigma). In addition, 132 secure redshifts were obtained for sources with no HST counterparts that were discovered in the MUSE data cubes by a blind search for emission-line features. In total, we present 1338 high quality redshifts, which is a factor of eight increase compared with the previously known spectroscopic redshifts in the same field. We assessed redshifts mainly with the spectral features [OII] at z<1.5 (473 objects) and Lya at 2.9<z<6.7 (692 objects). With respect to F775W magnitude, a 50% completeness is reached at 26.5 mag for ultra deep and 25.5 mag for deep fields, and the completeness remains >~20% up to 28-29 mag and ~27 mag, respectively. We used the determined redshifts to test continuum color selection (dropout) diagrams of high-z galaxies. The selection condition for F336W dropouts successfully captures ~80% of the targeted z~2.7 galaxies. However, for higher redshift selections (F435W, F606W, and F775W dropouts), the success rates decrease to ~20-40%. We empirically redefine the selection boundaries to make an attempt to improve them to ~60%. The revised boundaries allow bluer colors that capture Lya emitters with high Lya equivalent widths falling in the broadbands used for the color-color selection. Along with this paper, we release the redshift and line flux catalog.Comment: 26 pages, 26 figures, 4 tables, accepted for publication in A&A (MUSE UDF Series Paper II), the redshift catalogs will be available at the CD