Wood-inhabiting macrofungal assemblages in 43-year-old regenerating wet Eucalyptus Obliqua L'Her.Forest

This study focuses on the diversity and ecology of wood-inhabiting macrofungal species assemblages in a regenerating tall, wet, native Eucalyptus obliqua forest in southeast Tasmania, 43 years after natural and anthropogenic disturbances. Two plots subjected to "clearfell, burn and sow" silviculture were compared with two other nearby plots that had experienced wildfire. A total of 90 species was identified from 619 macro fungal records during six fortnightly visits between May and July 2010. The plots with abundant live Pomaderris ape tala trees in the understorey (i.e., those at Edwards Rd) had markedly different macrofungal assemblages from those with no or with sparse Pomaderris apetala (i.e., at Hartz Rd). This study provided evidence that a 43-year-old regenerating forest maintains a core of common wood-inhabiting macrofungal species irrespective of type of disturbance. Furthermore, species most frequently observed in older forests in Tasmania can also occur in younger managed forests if biological legacies such as large diameter wood, well-decayed wood, large living trees and a diversity of tree species remain after silvicultural treatment

Remission vs low disease activity: function, quality of life and structural outcomes in the Early Rheumatoid Arthritis Study and Network

Objectives To examine associations between function, quality of life and structural outcomes in patients achieving remission vs low disease activity in early RA. Methods Demographic, clinical and radiographic variables were collected at baseline and then annually from the Early Rheumatoid Arthritis Study (ERAS) and Early Rheumatoid Arthritis Network (ERAN) inception cohorts in routine care from 1986 to 2012. Disease activity was categorized: mean DAS28 score between years 1 and 5: remission [mean remission DAS (mRDAS) <2.6] or low [mean low DAS (mLDAS) 2.6–3.2]; sustained low/remission DAS28 (sLDAS/sRDAS) at years 1 and 2; and sustained Boolean remission (sBR) at years 1 and 2. Changes in HAQ and Short Form 36 Health Survey Questionnaire [SF-36; physical (PCS) and mental (MCS) component score]) and total Sharp van der Heijde (SvdH) scores for each disease activity category were modelled using multi-level models. Covariates included year of onset, age, gender and DMARD use at first visit. Results Of 2701 patients, 562 (21%) were categorized mRDAS, 330 (12%) mLDAS, 279 (10%) sRDAS, 203 (7.5%) sLDAS and 93 (3%) sBR. Patients categorized as mRDAS had increasingly divergent improved HAQ, SF-36 PCS, MCS and total SvdH scores compared with mLDAS (P-values 0.001 to <0.0001, all time points). Patients categorized as sRDAS had better HAQ, SF-36 PCS and MCS scores (P-values 0.05 to <0.0001, all time points) and SvdH scores (P = 0.05, years 3–5) over sLDAS. sBR was associated with better HAQ, and SF-36 PCS and MCS scores over sLDAS (P-values 0.002 to <0.0001, all time points). Conclusion These findings from routine care support ACR/EULAR guidelines that remission is a preferable goal over low disease activity in early RA.Peer reviewedFinal Published versio

Characteristics of intracellular peptidase and proteinase activities from the mycelium of a cord-forming wood decay fungus Serpula lacrymans

Serpula lacrymans is a basidiomycete cord-forming wood decay fungus which reallocates nitrogen within an extensive perennial mycelial system in response to spatial discontinuities in external nutrient supply. Intracellular stored protein is mobilised by conversion to amino acids at nutrient-poor sites within a mycelium or when a whole mycelium is starved. Intracellular peptidase and proteinase activities of the mycelium were investigated with the aim of identifying proteases specifically activated in response to a nitrogen demand. Mycelium for enzyme extraction grown as surface mats in static liquid culture was homogenised, and the extract used in assays for proteinase and peptidase with various synthetic peptide substrates conjugated to 4-nitroaniline. Activities against different substrates were characterised with respect to pH, inhibitor sensitivity, requirements for divalent metal ions, isoelectric point, and by changes in activities in starved mycelium. Four different activities were found, comprising two peptidases one of which had metalloprotease characteristics, a serine-type proteinase, and a proteinase active at pH 2.5 which was not affected by any of the inhibitors tried. Both the latter were most active in starved mycelium. Isoelectric focusing showed peaks with activities corresponding to the serine-type proteinase and one of the manganese-activated peptidases. Possible roles for these enzymes in nitrogen reallocation during mycelial foraging are discussed

Validation of methods for converting the original Disease Activity Score (DAS) to the DAS28

© The Author(s) 2018.The Disease Activity Score (DAS) is integral in tailoring the clinical management of rheumatoid arthritis (RA) patients and is an important measure in clinical research. Different versions have been developed over the years to improve reliability and ease of use. Combining the original DAS and the newer DAS28 data in both contemporary and historical studies is important for both primary and secondary data analyses. As such, a methodologically robust means of converting the old DAS to the new DAS28 measure would be invaluable. Using data from The Early RA Study (ERAS), a sub-sample of patients with both DAS and DAS28 data were used to develop new regression imputation formulas using the total DAS score (univariate), and using the separate components of the DAS score (multivariate). DAS were transformed to DAS28 using an existing formula quoted in the literature, and the newly developed formulas. Bland and Altman plots were used to compare the transformed DAS with the recorded DAS28 to ascertain levels of agreement. The current transformation formula tended to overestimate the true DAS28 score, particularly at the higher end of the scale. A formula which uses all separate components of the DAS was found to estimate the scores with a higher level of precision. A new formula is proposed that can be used by other early RA cohorts to convert the original DAS to DAS28.Peer reviewedFinal Published versio

Low genetic variability, female-biased dispersal and high movement rates in an urban population of Eurasian badgersMeles meles

1. Urban and rural populations of animals can differ in their behaviour, both in order to meet their ecological requirements and due to the constraints imposed by different environments. The study of urban populations can therefore offer useful insights into the behavioural flexibility of a species as a whole, as well as indicating how the species in question adapts to a specifically urban environment. 2. The genetic structure of a population can provide information about social structure and movement patterns that is difficult to obtain by other means. Using non-invasively collected hair samples, we estimated the population size of Eurasian badgers Meles meles in the city of Brighton, England, and calculated population-specific parameters of genetic variability and sex-specific rates of outbreeding and dispersal. 3. Population density was high in the context of badger densities reported throughout their range. This was due to a high density of social groups rather than large numbers of individuals per group. 4. The allelic richness of the population was low compared with other British populations. However, the rate of extra-group paternity and the relatively frequent (mainly temporary) intergroup movements suggest that, on a local scale, the population was outbred. Although members of both sexes visited other groups, there was a trend for more females to make intergroup movements. 5. The results reveal that urban badgers can achieve high densities and suggest that while some population parameters are similar between urban and rural populations, the frequency of intergroup movements is higher among urban badgers. In a wider context, these results demonstrate the ability of non-invasive genetic sampling to provide information about the population density, social structure and behaviour of urban wildlife

Evolution of floral symmetry

Flowers can be classified into two basic types according to their symmetry: regular flowers have more than one plane of symmetry and irregular flowers have only a single plane of symmetry. The irregular condition is thought to have evolved many times independently from the regular one: most commonly through the appearance of asymmetry along the dorso-ventral axis of the flower. In most cases, the irregular condition is associated with a particular type of inflorescence architecture. To understand the molecular mechanism and evolutionary origin of irregular flowers, we have been investigating genes controlling asymmetry in Antirrhinum. Several mutations have been described in Antirrhinum, a species with irregular flowers, that reduce or eliminate asymmetry along the dorso-ventral axis. We describe the nature of these mutations and how they may be used to analyse the molecular mechanisms underlying floral evolution

No wide spread of stellar ages in the Orion Nebula Cluster

The wide luminosity dispersion seen for stars at a given effective temperature in the H-R diagrams of young clusters and star forming regions is often interpreted as due to significant (~10 Myr) spreads in stellar contraction age. In the scenario where most stars are born with circumstellar discs, and that disc signatures decay monotonically (on average) over timescales of only a few Myr, then any such age spread should lead to clear differences in the age distributions of stars with and without discs. We have investigated large samples of stars in the Orion Nebula Cluster (ONC) using three methods to diagnose disc presence from infrared measurements. We find no significant difference in the mean ages or age distributions of stars with and without discs, consistent with expectations for a coeval population. Using a simple quantitative model we show that any real age spread must be smaller than the median disc lifetime. For a log-normal age distribution, there is an upper limit of <0.14 dex (at 99% confidence) to any real age dispersion, compared to the ~=0.4 dex implied by the H-R diagram. If the mean age of the ONC is 2.5 Myr, this would mean at least 95% of its low-mass stellar population has ages between 1.3--4.8 Myr. We suggest that the observed luminosity dispersion is caused by a combination of observational uncertainties and physical mechanisms that disorder the conventional relationship between luminosity and age for pre main-sequence stars. This means that individual stellar ages from the H-R diagram are unreliable and cannot be used to directly infer a star formation history. Irrespective of what causes the wide luminosity dispersion, the finding that any real age dispersion is less than the median disc lifetime argues strongly against star formation scenarios for the ONC lasting longer than a few Myr.Comment: To appear in MNRAS, 13 page

Rayleigh loops in the random-field Ising model on the Bethe lattice

We analyze the demagnetization properties of the random-field Ising model on the Bethe lattice focusing on the beahvior near the disorder induced phase transition. We derive an exact recursion relation for the magnetization and integrate it numerically. Our analysis shows that demagnetization is possible only in the continous high disorder phase, where at low field the loops are described by the Rayleigh law. In the low disorder phase, the saturation loop displays a discontinuity which is reflected by a non vanishing magnetization m_\infty after a series of nested loops. In this case, at low fields the loops are not symmetric and the Rayleigh law does not hold.Comment: 8pages, 6 figure