58 research outputs found
Identification and regulation of fusA, The polyketide synthase gene responsible for fusarin production in Fusarium fujikuroi
Fusarins are a class of mycotoxins of the polyketide family produced by different Fusarium species, including the gibberellin- producing fungus Fusarium fujikuroi. Based on sequence comparisons between polyketide synthase (PKS) enzymes for fusarin production in other Fusarium strains, we have identified the F. fujikuroi orthologue, called fusA. The participation of fusA in fusarin biosynthesis was demonstrated by targeted mutagenesis. Fusarin production is transiently stimulated by nitrogen avail- ability in this fungus, a regulation paralleled by the fusA mRNA levels in the cell. Illumination of the cultures results in a reduc- tion of the fusarin content, an effect partially explained by a high sensitivity of these compounds to light. Mutants of the fusA gene exhibit no external phenotypic alterations, including morphology and conidiation, except for a lack of the characteristic yellow and/or orange pigmentation of fusarins. Moreover, the fusA mutants are less efficient than the wild type at degrading cel- lophane on agar cultures, a trait associated with pathogenesis functions in Fusarium oxysporum. The fusA mutants, however, are not affected in their capacities to grow on plant tissues
Analysis of al-2 Mutations in Neurospora
The orange pigmentation of the fungus Neurospora crassa is due to the accumulation of the xanthophyll neurosporaxanthin and precursor carotenoids. Two key reactions in the synthesis of these pigments, the formation of phytoene from geranylgeranyl pyrophosphate and the introduction of β cycles in desaturated carotenoid products, are catalyzed by two domains of a bifunctional protein, encoded by the gene al-2. We have determined the sequence of nine al-2 mutant alleles and analyzed the carotenoid content in the corresponding strains. One of the mutants is reddish and it is mutated in the cyclase domain of the protein, and the remaining eight mutants are albino and harbor different mutations on the phytoene synthase (PS) domain. Some of the mutations are expected to produce truncated polypeptides. A strain lacking most of the PS domain contained trace amounts of a carotenoid-like pigment, tentatively identified as the squalene desaturation product diapolycopene. In support, trace amounts of this compound were also found in a knock-out mutant for gene al-2, but not in that for gene al-1, coding for the carotene desaturase. The cyclase activity of the AL-2 enzyme from two albino mutants was investigated by heterologous expression in an appropriately engineered E. coli strain. One of the AL-2 enzymes, predictably with only 20% of the PS domain, showed full cyclase activity, suggesting functional independence of both domains. However, the second mutant showed no cyclase activity, indicating that some alterations in the phytoene synthase segment affect the cyclase domain. Expression experiments showed a diminished photoinduction of al-2 transcripts in the al-2 mutants compared to the wild type strain, suggesting a synergic effect between reduced expression and impaired enzymatic activities in the generation of their albino phenotypes
The oxygenase CAO-1 of Neurospora crassa is a resveratrol cleavage enzyme.
The genome of the ascomycete Neurospora crassa encodes CAO-1 and CAO-2, two members of the carotenoid cleavage oxygenase family that target double bonds in different substrates. Previous studies demonstrated the role of CAO-2 in cleaving the C40 carotene torulene, a key step in the synthesis of the C35 apocarotenoid pigment neurosporaxanthin. In this work, we investigated the activity of CAO-1, assuming that it may provide retinal, the chromophore of the NOP-1 rhodopsin, by cleaving β-carotene. For this purpose, we tested CAO-1 activity with carotenoid substrates that were, however, not converted. In contrast and consistent with its sequence similarity to family members that act on stilbenes, CAO-1 cleaved the interphenyl Cα-Cβ double bond of resveratrol and its derivative piceatannol. CAO-1 did not convert five other similar stilbenes, indicating a requirement for a minimal number of unmodified hydroxyl groups in the stilbene background. Confirming its biological function in converting stilbenes, adding resveratrol led to a pronounced increase in cao-1 mRNA levels, while light, a key regulator of carotenoid metabolism, did not alter them. Targeted Δcao-1 mutants were not impaired by the presence of resveratrol, a phytoalexin active against different fungi, which did not significantly affect the growth and development of wild-type Neurospora. However, under partial sorbose toxicity, the Δcao-1 colonies exhibited faster radial growth than control strains in the presence of resveratrol, suggesting a moderate toxic effect of resveratrol cleavage products
Proceso de cuidados de enfermería aplicado en adulto con tuberculosis ganglionar y VIH
Caso clínico: Paciente adulto de 53 años de edad, con diagnóstico médico de tuberculosis ganglionar asociado a VIH, adelgazado y con dificultad para deambular, grado de dependencia I, se muestra ansioso y preocupado por su estado de salud, baja autoestima, inapetencia, además, sufre de estreñimiento, FV: PA: 130/80 mmHg, temperatura de 36,2 °C, FR: 16x’ SO2 94, pesa 50 kg y mide 1,75 m. En el resultado de la radiografía pulmonar presenta cavernas y tractos fibrosos. Método: Estudio descriptivo, se realizó en el Centro de Salud Delicias de Villa - Chorrillos, para recolectar los datos se aplicó un instrumento elaborado con los 11 patrones funcionales, previamente se solicitó autorización del paciente y familia. Resultados: La evolución del paciente es favorable, con mejor conocimiento del proceso de la enfermedad, buena adherencia al tratamiento, mejora en su estado nutricional y emocional, hay apoyo familiar. Conclusiones: Según los diagnósticos de enfermería, en el primer diagnóstico se logró el 90 % del objetivo, el 10 % está en proceso, en los diagnósticos 2, 3, 4 y 5 se logró un 60 % de los objetivos y el 40 % se encuentran en proceso; el paciente continúa en tratamiento
Collecting wild potato species (Solanum sect. Petota) in Peru to enhance genetic representation and fill gaps in ex situ collections
Crop wild relatives (CWRs) are important sources of novel genes, due to their high variability of response to biotic and abiotic stresses, which can be invaluable for crop genetic improvement programs. Recent studies have shown that CWRs are threatened by several factors, including changes in land-use and climate change. A large proportion of CWRs are underrepresented in genebanks, making it necessary to take action to ensure their long-term ex situ conservation. With this aim, 18 targeted collecting trips were conducted during 2017/2018 in the center of origin of potato (Solanum tuberosum L.), targeting 17 diverse ecological regions of Peru. This was the first comprehensive wild potato collection in Peru in at least 20 years and encompassed most of the unique habitats of potato CWRs in the country. A total of 322 wild potato accessions were collected as seed, tubers, and whole plants for ex situ storage and conservation. They belonged to 36 wild potato species including one accession of S. ayacuchense that was not conserved previously in any genebank. Most accessions required regeneration in the greenhouse prior to long-term conservation as seed. The collected accessions help reduce genetic gaps in ex situ conserved germplasm and will allow further research questions on potato genetic improvement and conservation strategies to be addressed. These potato CWRs are available by request for research, training, and breeding purposes under the terms of the International Treaty for Plant Genetic Resources for Food and Agriculture (ITPGRFA) from the Instituto Nacional de Innovacion Agraria (INIA) and the International Potato Center (CIP) in Lima-Peru
Fortalezas e inminencias en la central de esterilización: Percepción de las enfermeras
Introducción: Las enfermeras de central de esterilización, aunque no brindan un cuidado directo al paciente son las responsables de garantizar calidad en los procesos, la seguridad del paciente y la prevención de riesgos. Objetivo: Describir la percepción que tienen las enfermeras sobre sus fortalezas e inminencias al trabajar en la central de esterilización del Hospital Regional Lambayeque, Perú. Método: Estudio cualitativo descriptivo, la muestra fueron 12 enfermeras, se usó la entrevista semiestructurada y el análisis de contenido temático. Resultados principales: Fortalezas que garantizan la seguridad del paciente: Capacitación continua, trabajo en equipo y sistema de trazabilidad; Inminencias para la prevención de riesgo: Mantenimiento preventivo, abastecimiento de recursos y salud ocupacional. Conclusión general: A pesar de las fortalezas personales e institucionales, las enfermeras reconocen la necesidad de implementar un sistema de prevención de riesgos laborales, efectivizar un mantenimiento preventivo a equipos; pues se realiza solo un mantenimiento correctivo cuando ya ocurre el problema
The Fourteenth Data Release of the Sloan Digital Sky Survey: First Spectroscopic Data from the extended Baryon Oscillation Spectroscopic Survey and from the second phase of the Apache Point Observatory Galactic Evolution Experiment
The fourth generation of the Sloan Digital Sky Survey (SDSS-IV) has been in
operation since July 2014. This paper describes the second data release from
this phase, and the fourteenth from SDSS overall (making this, Data Release
Fourteen or DR14). This release makes public data taken by SDSS-IV in its first
two years of operation (July 2014-2016). Like all previous SDSS releases, DR14
is cumulative, including the most recent reductions and calibrations of all
data taken by SDSS since the first phase began operations in 2000. New in DR14
is the first public release of data from the extended Baryon Oscillation
Spectroscopic Survey (eBOSS); the first data from the second phase of the
Apache Point Observatory (APO) Galactic Evolution Experiment (APOGEE-2),
including stellar parameter estimates from an innovative data driven machine
learning algorithm known as "The Cannon"; and almost twice as many data cubes
from the Mapping Nearby Galaxies at APO (MaNGA) survey as were in the previous
release (N = 2812 in total). This paper describes the location and format of
the publicly available data from SDSS-IV surveys. We provide references to the
important technical papers describing how these data have been taken (both
targeting and observation details) and processed for scientific use. The SDSS
website (www.sdss.org) has been updated for this release, and provides links to
data downloads, as well as tutorials and examples of data use. SDSS-IV is
planning to continue to collect astronomical data until 2020, and will be
followed by SDSS-V.Comment: SDSS-IV collaboration alphabetical author data release paper. DR14
happened on 31st July 2017. 19 pages, 5 figures. Accepted by ApJS on 28th Nov
2017 (this is the "post-print" and "post-proofs" version; minor corrections
only from v1, and most of errors found in proofs corrected
Murciélagos y techos: Cruzando fronteras a través de la ciencia ciudadana
El Neotrópico es una de las regiones más diversas en el mundo, donde se han registrado cientos de especies de murciélagos y este número sigue ascendiendo gracias a los esfuerzos de investigación. A pesar de los distintos y valiosos servicios ecosistémicos que estas especies brindan (Boyles et al. 2011), los murciélagos enfrentan amenazas que ponen en riesgo su supervivencia, entre ellas se destacan la pérdida y fragmentación del hábitat (Frick et al. 2020). Estas amenazas han obligado a los murciélagos a buscar nuevos sitios donde habitar y, para algunas especies, principalmente insectívoras, las zonas urbanas poseen sitios con los recursos necesarios para sobrevivir, tales como alimento y refugio (Ávila-Flores y Fenton 2005; Jung y Kalko 2010; Jung y Threlfall 2016). En el momento que estas especies coexisten con los humanos, surge otra potencial amenaza que es el desconocimiento generado por la percepción errónea que existe sobre los murciélagos...Fil: Zaldaña Orantes, Karla. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: Rodríguez, Melissa E.. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: Raquel Alvarado-Larios. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: González Linares, Jorge. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: Campos Tobar, Zuleyma. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: Díaz, Carolina. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: Girón, Luis. Programa de Conservación de Murciélagos de El Salvador ; El SalvadorFil: Nuñez Rodríguez, Alvaro. Programa para la Conservación de los Murciélagos de Chile; ChileFil: Chang, Clemente Beltrán. Programa para la Conservación de los Murciélagos de Chile; ChileFil: Damino, María Verónica. Programa de Conservación de los Murciélagos de Argentina; ArgentinaFil: Di Domenica, Violeta. Programa de Conservación de los Murciélagos de Argentina; ArgentinaFil: Olmedo, María Luz. Programa de Conservación de los Murciélagos de Argentina; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo. Programa de Investigación de Biodiversidad Argentina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sánchez, Tatiana. Programa de Conservación de los Murciélagos de Argentina; ArgentinaFil: Arévalo, Ana Lucía. Programa para la Conservación de los Murciélagos de Guatemala; GuatemalaFil: Nuñez, Lourdes. Programa para la Conservación de los Murciélagos de Guatemala; GuatemalaFil: Mejía, David. Programa de Conservación de Murciélagos de Honduras; HondurasFil: Aguirre, Gabriel. Programa de Conservación de Murciélagos de Nicaragua; NicaraguaFil: Saldaña, Octavio. Programa de Conservación de Murciélagos de Nicaragua; NicaraguaFil: Serrano, Alejandra. Programa de Conservación de Murciélagos de Nicaragua; NicaraguaFil: Chitaro, Santiago. Programa para la Conservación de los Murciélagos de Uruguay; UruguayFil: Martínez, Yaniré. Programa de Conservación de Murciélagos de Puerto Rico; Puerto RicoFil: Santiago, Miguel. Programa de Conservación de Murciélagos de la República
Dominicana; República DominicanaFil: Mateo Jiménez, Amelia L.. Programa de Conservación de Murciélagos de la República
Dominicana; República DominicanaFil: Sánchez Calderón, Ricardo. Programa para la Conservación de los Murciélagos de Costa Rica; Costa RicaFil: Oviedo Cortés, Gabriel. Programa para la Conservación de los Murciélagos de Costa Rica; Costa RicaFil: Guido Solano, Francinie. Programa para la Conservación de los Murciélagos de Costa Rica; Costa Ric
The Caldera. No. 26
“La PALABRA tiene PODER”, más allá del lema que nos convocó en la décima versión del Concurso Inter colegiado de Oratoria, que en el marco de ULIBRO 2023, se llevó a cabo en NEOMUNDO, el 1 de septiembre pasado, esta frase es una profunda convicción que ha movido al Área de Lengua Castellana del Instituto Caldas, a propiciar y mantener este espacio en el que nuestros estudiantes son los protagonistas. Este año, al tratarse de una celebración especial, quisimos que el tema fundamental fuera el inmenso PODER que tiene la PALABRA; la palabra que construye, que transforma, que edifica,
que fortalece, que acompaña, que “abriga”, a diario, al ser humano; en un mundo en el que se debe volver a entender y vivenciar, en el hogar, en la escuela, en la sociedad, el poder transformador de vocablos tan importantes como lo son: “Familia, Educación, Vida, Diversidad, Respeto, Literatura, Escritura, Creatividad, Lectura, Inclusión, Paz, Igualdad Social, Democracia, Ciudadanía y Diálogo”, la palabra cobró fuerza en las voces de nuestros educandos; de esta manera, analizar estos temas tiene que ver necesariamente con los “Futuros posibles”, que planteó la Gran Feria del Libro, ULIBRO, en su vigésima primera versión, para que sigamos trabajando por una sociedad mejor, razón de ser de todas las instituciones educativas del mundo entero.“The WORD has POWER”, beyond the motto that summoned us in the tenth version of the Intercollegiate Oratory Contest, which within the framework of ULIBRO 2023, was held in NEOMUNDO, last September 1, this phrase is a deep conviction that has moved the Spanish Language Area of the Caldas Institute to promote and maintain this space in which our students are the protagonists. This year, as it is a special celebration, we wanted the fundamental theme to be the immense POWER that the WORD has; the word that builds, that transforms, that edifies,
that strengthens, that accompanies, that “shelters”, daily, the human being; in a world in which we must understand and experience again, at home, at school, in society, the transformative power of words as important as: “Family, Education, Life, Diversity, Respect, Literature, Writing, Creativity, Reading, Inclusion, Peace, Social Equality, Democracy, Citizenship and Dialogue”, the word gained strength in the voices of our students; In this way, analyzing these issues necessarily has to do with the “Possible Futures”, which the Great Book Fair, ULIBRO, proposed in its twenty-first version, so that we continue working for a better society, the reason for being of all institutions. . educational institutions around the world.Modalidad Presencia
- …