Galvanic Liquid Applied Coating Development for Protection of Steel in Concrete

Corrosion of reinforcing steel in concrete is a major problem affecting NASA facilities at Kennedy Space Center (KSC), other government agencies, and the general public. Problems include damage to KSC launch support structures, transportation and marine infrastructures, as well as building structures. A galvanic liquid applied coating was developed at KSC in order to address this problem. The coating is a non-epoxy metal rich ethyl silicate liquid coating. The coating is applied as a liquid from initial stage to final stage. Preliminary data shows that this coating system exceeds the NACE 100 millivolt shift criterion. The remainder of the paper details the development of the coating system through the following phases: Phase I: Development of multiple formulations of the coating to achieve easy application characteristics, predictable galvanic activity, long-term protection, and minimum environmental impact. Phase II: Improvement of the formulations tested in Phase I including optimization of metallic loading as well as incorporation of humectants for continuous activation. Phase III: Application and testing of improved formulations on the test blocks. Phase IV: Incorporation of the final formulation upgrades onto large instrumented structures (slabs)

Unconformities and Age Relationships, Tongue River and Older Members of the Fort Union Formation (Paleocene), Western Williston Basin, U.S.A.

An unconformable relationship is observed within the Paleocene Fort Union Formation in the western Williston Basin at the contact between the Tongue River Member and the underlying Lebo and Ludlow Members. Isotopic dates and pollen biozone data reported here are integrated with previously published data. A new correlation of these facies results in a revised history of localized depositional and tectonic events. One unconformity occurs at this lithological contact in the Pine Hills (PH), Terry Badlands (TB), and Ekalaka (E) areas west of the Cedar Creek anticline (CCA), and another unconformity occurs at the same lithological contact in the Little Missouri River (LMR) area east of the CCA. The two unconformities differ in age by about two million years. The older is the U2 and the younger is the U3 , which initially were recognized in the Ekalaka area of southeastern Montana (Belt et al., 2002). The U2 crops out in the TB, PH, and E areas, where at least 85 m of Tongue River strata bearing palynomorphs characteristic of biozone P-3 are found above the unconformity. Radiometric dates from strata (bearing palynomorphs characteristic of biozone P-2) below the U2 range in age from 64.0 to 64.73 Ma. The U2 unconformity west of the CCA thus occurs in strata near the base of the lower P-3 biozone. The U3 crops out in the LMR area (east of the CCA), where only 13 m of strata characterized by the P-3 pollen biozone occur above it. Radiometric dates from an ash \u3c1 m above the U3 in that area range in age from 61.03 to 61.23 Ma, and the P-3/P-4 pollen biozone boundary is located 13 m above the ashes. The U3 thus occurs in strata characterized by upper parts of the P-3 pollen biozone east of the CCA. The U3 is also identifiable in the middle of the ca. 200 m-thick Tongue River Member west of the CCA, where mammal sites 40 to 80 m above it are Tiffanian-3 in age. The strata below this unconformity are tilted gently to the northwest; strata above the unconformity are flat lying. This mid Tongue River unconformity probably correlates with the unconformity at the base of the Tongue River Member in the LMR area east of the CCA, where a Ti-2 mammal site (the “X-X” locality) occurs \u3c10 m above it. Depositional and tectonic events can be summarized using North American Mammal Age nomenclature as a relative time scale. From latest Cretaceous through Puercan time, paleodrainage was toward the east or southeast, in the direction of the Cannonball Sea. The Black Hills did not serve as an obstruction at that time. During early Torrejonian time, the Miles City arch (MCA) and Black Hills were uplifted and partially eroded, leading to the U2 unconformity. When deposition resumed, paleodrainages shifted to a northeasterly course. During middle and late Torrejonian time, facies of the lower Tongue River (“Dominy”) sequence and the Ekalaka Member of the Fort Union Formation were deposited in the middle of a subbasin between the MCA and the CCA. Simultaneously, smectite-rich components of the Ludlow Member were being deposited east of the CCA. During latest Torrejonian time, uplift of the Black Hills tilted the “Dominy” sequence toward the northwest and local erosion led to the U3 unconformity. Following this tilting, during Tiffanian time, deposition of the upper Tongue River (“Knobloch”) sequence shows continuity from western North Dakota across eastern Montana and into the northern Powder River Basin

Evidence for Marine Influence on a Low-Gradient Coastal Plain: Ichnology and Invertebrate Paleontology of the Lower Tongue River Member (Fort Union Formation, Middle Paleocene), Western Williston Basin, U.S.A.

The Paleocene Tongue River Member of the Fort Union Formation contains trace-fossil associations indicative of marine influence in otherwise freshwater facies. The identified ichnogenera include: Arenicolites, Diplocraterion, Monocraterion, Ophiomorpha, Rhizocorallium, Skolithos linearis, Teichichnus, Thalassinoides, and one form of uncertain affinity. Two species of the marine diatom Coscinodiscus occur a few meters above the base of the member. The burrows occur in at least five discrete, thin, rippled, fine-grained sandstone beds within the lower 85 m of the member west of the Cedar Creek anticline (CCA) in the Signal Butte, Terry Badlands, and Pine Hills areas. Two discrete burrowed beds are found in the lower 10 m of the member east of the CCA in the little Missouri River area. Abundant freshwater ostracodes include Bisulcocypridea arvadensis, Candona, and Cypridopsis. Freshwater bivalves include Plesielliptio and Pachydon mactriformis. We recognize four fossil assemblages that represent fluvio-lacustrine, proximal estuarine, central estuarine, and distal estuarine environments. Biostratal alternations between fresh- and brackish-water assemblages indicate that the Tongue River Member was deposited along a low-gradient coastal plain that was repeatedly inundated from the east by the Cannonball Sea. The existence of marine-influenced beds in the Tongue River Member invalidates the basis for the Slope Formation

Improved estimates of preindustrial biomass burning reduce the magnitude of aerosol climate forcing in the Southern Hemisphere.

Fire plays a pivotal role in shaping terrestrial ecosystems and the chemical composition of the atmosphere and thus influences Earth's climate. The trend and magnitude of fire activity over the past few centuries are controversial, which hinders understanding of preindustrial to present-day aerosol radiative forcing. Here, we present evidence from records of 14 Antarctic ice cores and 1 central Andean ice core, suggesting that historical fire activity in the Southern Hemisphere (SH) exceeded present-day levels. To understand this observation, we use a global fire model to show that overall SH fire emissions could have declined by 30% over the 20th century, possibly because of the rapid expansion of land use for agriculture and animal production in middle to high latitudes. Radiative forcing calculations suggest that the decreasing trend in SH fire emissions over the past century largely compensates for the cooling effect of increasing aerosols from fossil fuel and biofuel sources

Improved estimates of preindustrial biomass burning reduce the magnitude of aerosol climate forcing in the Southern Hemisphere.

Fire plays a pivotal role in shaping terrestrial ecosystems and the chemical composition of the atmosphere and thus influences Earth's climate. The trend and magnitude of fire activity over the past few centuries are controversial, which hinders understanding of preindustrial to present-day aerosol radiative forcing. Here, we present evidence from records of 14 Antarctic ice cores and 1 central Andean ice core, suggesting that historical fire activity in the Southern Hemisphere (SH) exceeded present-day levels. To understand this observation, we use a global fire model to show that overall SH fire emissions could have declined by 30% over the 20th century, possibly because of the rapid expansion of land use for agriculture and animal production in middle to high latitudes. Radiative forcing calculations suggest that the decreasing trend in SH fire emissions over the past century largely compensates for the cooling effect of increasing aerosols from fossil fuel and biofuel sources

A Comparison of Online versus On-site Training in Health Research Methodology: A Randomized Study

<p>Abstract</p> <p>Background</p> <p>Distance learning may be useful for building health research capacity. However, evidence that it can improve knowledge and skills in health research, particularly in resource-poor settings, is limited. We compared the impact and acceptability of teaching two distinct content areas, Biostatistics and Research Ethics, through either on-line distance learning format or traditional on-site training, in a randomized study in India. Our objective was to determine whether on-line courses in Biostatistics and Research Ethics could achieve similar improvements in knowledge, as traditional on-site, classroom-based courses.</p> <p>Methods</p> <p><it>Subjects: </it>Volunteer Indian scientists were randomly assigned to one of two arms.</p> <p><it>Intervention: </it>Students in Arm 1 attended a 3.5-day on-site course in Biostatistics and completed a 3.5-week on-line course in Research Ethics. Students in Arm 2 attended a 3.5-week on-line course in Biostatistics and 3.5-day on-site course in Research Ethics. For the two course formats, learning objectives, course contents and knowledge tests were identical.</p> <p><it>Main Outcome Measures: </it>Improvement in knowledge immediately and 3-months after course completion, compared to baseline.</p> <p>Results</p> <p>Baseline characteristics were similar in both arms (n = 29 each). Median knowledge score for Biostatistics increased from a baseline of 49% to 64% (p < 0.001) 3 months after the on-site course, and from 48% to 63% (p = 0.009) after the on-line course. For the on-site Research Ethics course, median score increased from 69% to 83% (p = 0.005), and for the on-line Research Ethics course from 62% to 80% (p < 0.001). Three months after the course, median gains in knowledge scores remained similar for the on-site and on-line platforms for both Biostatistics (16% vs. 12%; p = 0.59) and Research Ethics (17% vs. 13%; p = 0.14).</p> <p>Conclusion</p> <p>On-line and on-site training formats led to marked and similar improvements of knowledge in Biostatistics and Research Ethics. This, combined with logistical and cost advantages of on-line training, may make on-line courses particularly useful for expanding health research capacity in resource-limited settings.</p

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp5 main protease

The coronavirus 2019 (COVID-19) pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), spread around the world with unprecedented health and socio-economic effects for the global population. While different vaccines are now being made available, very few antiviral drugs have been approved. The main viral protease (nsp5) of SARS-CoV-2 provides an excellent target for antivirals, due to its essential and conserved function in the viral replication cycle. We have expressed, purified and developed assays for nsp5 protease activity. We screened the nsp5 protease against a custom chemical library of over 5000 characterised pharmaceuticals. We identified calpain inhibitor I and three different peptidyl fluoromethylketones (FMK) as inhibitors of nsp5 activity in vitro, with IC(50) values in the low micromolar range. By altering the sequence of our peptidomimetic FMK inhibitors to better mimic the substrate sequence of nsp5, we generated an inhibitor with a subnanomolar IC(50). Calpain inhibitor I inhibited viral infection in monkey-derived Vero E6 cells, with an EC(50) in the low micromolar range. The most potent and commercially available peptidyl-FMK compound inhibited viral growth in Vero E6 cells to some extent, while our custom peptidyl FMK inhibitor offered a marked antiviral improvement

Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of Nsp14 RNA cap methyltransferase

The COVID-19 pandemic has presented itself as one of the most critical public health challenges of the century, with SARS-CoV-2 being the third member of the Coronaviridae family to cause a fatal disease in humans. There is currently only one antiviral compound, remdesivir, that can be used for the treatment of COVID-19. To identify additional potential therapeutics, we investigated the enzymatic proteins encoded in the SARS-CoV-2 genome. In this study, we focussed on the viral RNA cap methyltransferases, which play key roles in enabling viral protein translation and facilitating viral escape from the immune system. We expressed and purified both the guanine-N7 methyltransferase nsp14, and the nsp16 2′-O-methyltransferase with its activating cofactor, nsp10. We performed an in vitro high-throughput screen for inhibitors of nsp14 using a custom compound library of over 5000 pharmaceutical compounds that have previously been characterised in either clinical or basic research. We identified four compounds as potential inhibitors of nsp14, all of which also showed antiviral capacity in a cell-based model of SARS-CoV-2 infection. Three of the four compounds also exhibited synergistic effects on viral replication with remdesivir

Assessing the carcinogenic potential of low-dose exposures to chemical mixtures in the environment: the challenge ahead.

Lifestyle factors are responsible for a considerable portion of cancer incidence worldwide, but credible estimates from the World Health Organization and the International Agency for Research on Cancer (IARC) suggest that the fraction of cancers attributable to toxic environmental exposures is between 7% and 19%. To explore the hypothesis that low-dose exposures to mixtures of chemicals in the environment may be combining to contribute to environmental carcinogenesis, we reviewed 11 hallmark phenotypes of cancer, multiple priority target sites for disruption in each area and prototypical chemical disruptors for all targets, this included dose-response characterizations, evidence of low-dose effects and cross-hallmark effects for all targets and chemicals. In total, 85 examples of chemicals were reviewed for actions on key pathways/mechanisms related to carcinogenesis. Only 15% (13/85) were found to have evidence of a dose-response threshold, whereas 59% (50/85) exerted low-dose effects. No dose-response information was found for the remaining 26% (22/85). Our analysis suggests that the cumulative effects of individual (non-carcinogenic) chemicals acting on different pathways, and a variety of related systems, organs, tissues and cells could plausibly conspire to produce carcinogenic synergies. Additional basic research on carcinogenesis and research focused on low-dose effects of chemical mixtures needs to be rigorously pursued before the merits of this hypothesis can be further advanced. However, the structure of the World Health Organization International Programme on Chemical Safety 'Mode of Action' framework should be revisited as it has inherent weaknesses that are not fully aligned with our current understanding of cancer biology

Money, (Co)Production and Power in Digital

This article discusses the contribution of critical political economy approaches to digital journalism studies and argues that these offer important correctives to celebratory perspectives. The first part offers a review and critique of influential claims arising from self-styled new studies of convergence culture, media and creative industries. The second part discusses the contribution of critical political economy in examining digital journalism and responding to celebrant claims. The final part reflects on problems of restrictive normativity and other limitations within media political economy perspectives and considers ways in which challenges might be addressed by more synthesising approaches. The paper proposes developing radical pluralist, media systems and comparative analysis, and advocates drawing on strengths in both political economy and culturalist traditions to map and evaluate practices across all sectors of digital journalism