4 research outputs found
Study of flows in physiologically realistic patient specific airway models
The paper presents the development of techniques to study flows in human conducting airways aimed at improving understanding of underlying mechanisms in asthma and Chronic Obstructive Pulmonary Disease (COPD). The models developed are made through additive layer manufacturing (ALM) of segmented lung Computed Tomography (CT) scans. The flow dynamics in these models are studied using time-resolved stereo PIV. Results are presented for various lung models including different geometries as well as different material properties to allow differentiating between flow effects due to geometry and wall compliance
Strand separation establishes a sustained lock at the Tus-Ter replication fork barrier
The bidirectional replication of a circular chromosome by many bacteria necessitates proper termination to avoid the head-on collision of the opposing replisomes. In Escherichia coli, replisome progression beyond the termination site is prevented by Tus proteins bound to asymmetric Ter sites. Structural evidence indicates that strand separation on the blocking (nonpermissive) side of Tus-Ter triggers roadblock formation, but biochemical evidence also suggests roles for protein-protein interactions. Here DNA unzipping experiments demonstrate that nonpermissively oriented Tus-Ter forms a tight lock in the absence of replicative proteins, whereas permissively oriented Tus-Ter allows nearly unhindered strand separation. Quantifying the lock strength reveals the existence of several intermediate lock states that are impacted by mutations in the lock domain but not by mutations in the DNA-binding domain. Lock formation is highly specific and exceeds reported in vivo efficiencies. We postulate that protein-protein interactions may actually hinder, rather than promote, proper lock formation.Accepted Author ManuscriptBN/Nynke Dekker LabBN/Cees Dekker LabBN/Martin Depken La
Measurement of the diffractive cross-section in deep inelastic scattering
Diffractive scattering of , where is either a
proton or a nucleonic system with ~GeV has been measured in deep
inelastic scattering (DIS) at HERA. The cross section was determined by a novel
method as a function of the c.m. energy between 60 and 245~GeV
and of the mass of the system up to 15~GeV at average values of
14 and 31~GeV. The diffractive cross section is,
within errors, found to rise linearly with . Parameterizing the
dependence by the form d\sigma^{diff}/dM_X \propto
(W^2)^{(2\overline{\mbox{\alpha_{_{I\hspace{-0.2em}P}}}} -2)} the DIS data
yield for the pomeron trajectory
\overline{\mbox{\alpha_{_{I\hspace{-0.2em}P}}}} = 1.23 \pm 0.02(stat) \pm
0.04 (syst) averaged over in the measured kinematic range assuming the
longitudinal photon contribution to be zero. This value for the pomeron
trajectory is substantially larger than
\overline{\mbox{\alpha_{_{I\hspace{-0.2em}P}}}} extracted from soft
interactions. The value of \overline{\mbox{\alpha_{_{I\hspace{-0.2em}P}}}}
measured in this analysis suggests that a substantial part of the diffractive
DIS cross section originates from processes which can be described by
perturbative QCD. From the measured diffractive cross sections the diffractive
structure function of the proton F^{D(3)}_2(\beta,Q^2,
\mbox{x_{_{I\hspace{-0.2em}P}}}) has been determined, where is the
momentum fraction of the struck quark in the pomeron. The form F^{D(3)}_2 =
constant \cdot (1/ \mbox{x_{_{I\hspace{-0.2em}P}}})^a gives a good fit to
the data in all and intervals with $a = 1.46 \pm 0.04 (stat) \pmComment: 45 pages, including 16 figure