Soil Moisture Estimations Based on Airborne CAROLS L-Band Microwave Data

International audienceThe SMOS satellite mission, launched in 2009, allows global soil moisture estimations to be made using the L-band Microwave Emission of the Biosphere (L-MEB) model, which simulates the L-band microwave emissions produced by the soil-vegetation layer. This model was calibrated using various sources of in situ and airborne data. In the present study, we propose to evaluate the L-MEB model on the basis of a large set of airborne data, recorded by the CAROLS radiometer during the course of 20 flights made over South West France (the SMOSMANIA site), and supported by simultaneous soil moisture measurements, made in 2009 and 2010. In terms of volumetric soil moisture, the retrieval accuracy achieved with the L-MEB model, with two default roughness parameters, ranges between 8% and 13%. Local calibrations of the roughness parameter, using data from the 2009 flights for different areas of the site, allowed an accuracy of approximately 5.3% to be achieved with the 2010 CAROLS data. Simultaneously we estimated the vegetation optical thickness (t) and we showed that, when roughness is locally adjusted, MODIS NDVI values are correlated (R2 = 0.36) to t. Finally, as a consequence of the significant influence of the roughness parameter on the estimated absolute values of soil moisture, we propose to evaluate the relative variability of the soil moisture, using a default soil roughness parameter. The soil moisture variations are estimated with an uncertainty of approximately 6%

Cassini VIMS and Altimeter Joint Study of Titan Surface

The joint NASA-ESA-ASI Cassini- Huygens mission reached the saturnian system on July 1st 2004. It started the observations of Saturn's environment including its atmosphere, rings, and satellites (Phoebe, Iapetus and Titan). Titan, one of the primary scientific interests of the mission, is veiled by an ubiquitous thick haze [1]. Its surface is unreachable to ultraviolet and visible wavelengths, but can be seen in some infrared atmospheric windows and for greater wavelengths, in the case of an unclouded low atmosphere [2,3]. Onboard the Cassini spacecraft, the VIMS (Visual and Infrared Mapping Spectrometer) instrument has already proved to be able to successfully pierce the veil of the hazy moon and image its surface in the infrared wavelengths, taking hyperspectral images in the range 0.4 to 5.2 ?m. Since July 2004, VIMS acquired image cubes with spatial resolution ranging from a few tens of kilometers down to less than one kilometer per pixel, demonstrating its capability for mapping more than 70% of Titan's surface and studying its composition and geology [4,5,6,7,8,9,10,11]. Also in the Cassini orbiter payload is the Ku-band RADAR experiment that can operate in altimeter mode. Exclusively dedicated to Titan's observations, this second active mode has been designed primarily to retrieve Titan's surface elevation and study its topography. We present here the comparative analysis of the altimeter track recorded during the first Titan flyby (26 October 2004, tagged TA) and VIMS images over the same regions acquired during the 13th flyby (30 April 2006). In particular, we present here the first nontopographic analysis of Cassini altimeter data along with a tentative correlation with VIMS observations

Cassini VIMS and Altimeter Joint Study of Titan Surface

The joint NASA-ESA-ASI Cassini- Huygens mission reached the saturnian system on July 1st 2004. It started the observations of Saturn's environment including its atmosphere, rings, and satellites (Phoebe, Iapetus and Titan). Titan, one of the primary scientific interests of the mission, is veiled by an ubiquitous thick haze [1]. Its surface is unreachable to ultraviolet and visible wavelengths, but can be seen in some infrared atmospheric windows and for greater wavelengths, in the case of an unclouded low atmosphere [2,3]. Onboard the Cassini spacecraft, the VIMS (Visual and Infrared Mapping Spectrometer) instrument has already proved to be able to successfully pierce the veil of the hazy moon and image its surface in the infrared wavelengths, taking hyperspectral images in the range 0.4 to 5.2 ?m. Since July 2004, VIMS acquired image cubes with spatial resolution ranging from a few tens of kilometers down to less than one kilometer per pixel, demonstrating its capability for mapping more than 70% of Titan's surface and studying its composition and geology [4,5,6,7,8,9,10,11]. Also in the Cassini orbiter payload is the Ku-band RADAR experiment that can operate in altimeter mode. Exclusively dedicated to Titan's observations, this second active mode has been designed primarily to retrieve Titan's surface elevation and study its topography. We present here the comparative analysis of the altimeter track recorded during the first Titan flyby (26 October 2004, tagged TA) and VIMS images over the same regions acquired during the 13th flyby (30 April 2006). In particular, we present here the first nontopographic analysis of Cassini altimeter data along with a tentative correlation with VIMS observations

Monitoring of water and carbon fluxes using a land data assimilation system: a case study for southwestern France

International audienceA Land Data Assimilation System (LDAS) able to ingest surface soil moisture (SSM) and Leaf Area Index (LAI) observations is tested at local scale to increase prediction accuracy for water and carbon fluxes. The ISBAA-gs Land Surface Model (LSM) is used together with LAI and the soil water content observations of a grassland at the SMOSREX experimental site in southwestern France for a seven-year period (2001-2007). Three configurations corresponding to contrasted model errors are considered: (1) best case (BC) simulation with locally observed atmospheric variables and model parameters, and locally observed SSM and LAI used in the assimilation, (2) same as (1) but with the precipitation forcing set to zero, (3) real case (RC)simulation with atmospheric variables and model parameters derived from regional atmospheric analyses and from climatological soil and vegetation properties, respectively. In configuration (3) two SSM time series are considered: the observed SSM using Thetaprobes, and SSM derived from the LEWIS L-band radiometer located 15m above the ground. Performance of the LDAS is examined in the three configurations described above with either one variable (either SSM or LAI) or two variables (both SSM and LAI) assimilated. The joint assimilation of SSM and LAI has a positive impact on the carbon, water, and heat fluxes. It represents a greater impact than assimilating one variable (either LAI or SSM). Moreover, the LDAS is able to counterbalance large errors in the precipitation forcing given as input to the model

Genetic dissection of early endosomal recycling highlights a TORC1-independent role for Rag GTPases

Endocytosed cell surface membrane proteins rely on recycling pathways for their return to the plasma membrane. Although endosome-to-plasma membrane recycling is critical for many cellular processes, much of the required machinery is unknown. We discovered that yeast has a recycling route from endosomes to the cell surface that functions efficiently after inactivation of the sec7-1 allele of Sec7, which controls transit through the Golgi. A genetic screen based on an engineered synthetic reporter that exclusively follows this pathway revealed that recycling was subject to metabolic control through the Rag GTPases Gtr1 and Gtr2, which work downstream of the exchange factor Vam6. Gtr1 and Gtr2 control the recycling pathway independently of TORC1 regulation through the Gtr1 interactor Ltv1. We further show that the early-endosome recycling route and its control though the Vam6 > Gtr1/Gtr2 > Ltv1 pathway plays a physiological role in regulating the abundance of amino acid transporters at the cell surface

Cellular factors determining the [URE3] prion propagation on the Saccharomyces cerevisiae yeast

Une protéine prion peut adopter deux conformations distinctes, l’une cellulaire et l’autre prion. La conformation prion est le résultat de son agrégation en fibre amyloïde. Cette fibre est le support de l’information prion à partir duquel les isoformes cellulaires sont convertis en forme prion de façon autocatalytique. La transmission de l’information prion repose donc sur la transmission de cette fibre au cours des divisions cellulaires, qui est réalisée par de petits polymères. Ceux-ci sont le résultat d’un équilibre entre la fragmentation et la polymérisation de la fibre. Une perturbation de cet équilibre provoque une agrégation massive de la protéine prion, menant à la perte de l’information prion.L’objectif de ma thèse était de comprendre ce qui définit in vivo la transmission du prion. Mon modèle d’étude est la protéine Ure2p propageant le prion [URE3] dans la levure S. cerevisiae. J’ai montré que la concentration cellulaire d’Ure2p détermine la vitesse d’agrégation de la protéine prion et donc son efficacité de transmission. En effet, de trop fortes concentrations cellulaires sont incompatibles avec la propagation du prion. La concentration cellulaire d’Ure2p définit également la diversité des souches prions. Un crible génétique m’a permit de mettre en évidence que la présence de séquences centromériques surnuméraires dans la cellule interfère avec la transmission du prion [URE3]. Le même phénomène est observé avec une augmentation du niveau de ploïdie de la cellule. Dans les deux cas, la surexpression du chaperon Hsp104 restaure une propagation normale du prion.A prion protein can adopt two distinct conformations, one cellular and one prion. Prion conformation is the result of its aggregation into amyloid fibers. This fiber is the support of the prion information from which the cellular isoforms are converted into prion form by autocatalytic manner. The prion information transmission is therefore based on the transmission of this fiber during cell division, which is done by small polymers. These are the result of a balance between fragmentation and polymerization of the fiber. A disturbance of this balance causes a massive aggregation of the prion protein, leading to the prion information loss.The objective of my thesis was to understand what defined in vivo the prion transmission. My studying model was the Ure2p protein propagating the [URE3] prion in S. cerevisiae yeast. I showed that the Ure2p cellular concentration determined the aggregation speed of the prion protein and thus its transmission efficiency. Indeed, too high cellular concentrations are incompatible with the prion propagation. The cellular concentration of Ure2p also defines the prion strains diversity. A genetic screen allowed me to highlight that the presence of centrometric supernumerary sequences in the cell interferes with the [URE3] prion transmission. The same phenomenon is observed with an increase in the cell ploidy. In both cases, overexpression of the Hsp104 chaperone restores normal prion propagation

Cellular factors determining the [URE3] prion propagation on the Saccharomyces cerevisiae yeast

Une protéine prion peut adopter deux conformations distinctes, l’une cellulaire et l’autre prion. La conformation prion est le résultat de son agrégation en fibre amyloïde. Cette fibre est le support de l’information prion à partir duquel les isoformes cellulaires sont convertis en forme prion de façon autocatalytique. La transmission de l’information prion repose donc sur la transmission de cette fibre au cours des divisions cellulaires, qui est réalisée par de petits polymères. Ceux-ci sont le résultat d’un équilibre entre la fragmentation et la polymérisation de la fibre. Une perturbation de cet équilibre provoque une agrégation massive de la protéine prion, menant à la perte de l’information prion.L’objectif de ma thèse était de comprendre ce qui définit in vivo la transmission du prion. Mon modèle d’étude est la protéine Ure2p propageant le prion [URE3] dans la levure S. cerevisiae. J’ai montré que la concentration cellulaire d’Ure2p détermine la vitesse d’agrégation de la protéine prion et donc son efficacité de transmission. En effet, de trop fortes concentrations cellulaires sont incompatibles avec la propagation du prion. La concentration cellulaire d’Ure2p définit également la diversité des souches prions. Un crible génétique m’a permit de mettre en évidence que la présence de séquences centromériques surnuméraires dans la cellule interfère avec la transmission du prion [URE3]. Le même phénomène est observé avec une augmentation du niveau de ploïdie de la cellule. Dans les deux cas, la surexpression du chaperon Hsp104 restaure une propagation normale du prion.A prion protein can adopt two distinct conformations, one cellular and one prion. Prion conformation is the result of its aggregation into amyloid fibers. This fiber is the support of the prion information from which the cellular isoforms are converted into prion form by autocatalytic manner. The prion information transmission is therefore based on the transmission of this fiber during cell division, which is done by small polymers. These are the result of a balance between fragmentation and polymerization of the fiber. A disturbance of this balance causes a massive aggregation of the prion protein, leading to the prion information loss.The objective of my thesis was to understand what defined in vivo the prion transmission. My studying model was the Ure2p protein propagating the [URE3] prion in S. cerevisiae yeast. I showed that the Ure2p cellular concentration determined the aggregation speed of the prion protein and thus its transmission efficiency. Indeed, too high cellular concentrations are incompatible with the prion propagation. The cellular concentration of Ure2p also defines the prion strains diversity. A genetic screen allowed me to highlight that the presence of centrometric supernumerary sequences in the cell interferes with the [URE3] prion transmission. The same phenomenon is observed with an increase in the cell ploidy. In both cases, overexpression of the Hsp104 chaperone restores normal prion propagation

Facteurs cellulaires déterminant la propagation du prion [URE3] dans la levure Saccharomyces cerevisiae

Une protéine prion peut adopter deux conformations distinctes, l une cellulaire et l autre prion. La conformation prion est le résultat de son agrégation en fibre amyloïde. Cette fibre est le support de l information prion à partir duquel les isoformes cellulaires sont convertis en forme prion de façon autocatalytique. La transmission de l information prion repose donc sur la transmission de cette fibre au cours des divisions cellulaires, qui est réalisée par de petits polymères. Ceux-ci sont le résultat d un équilibre entre la fragmentation et la polymérisation de la fibre. Une perturbation de cet équilibre provoque une agrégation massive de la protéine prion, menant à la perte de l information prion.L objectif de ma thèse était de comprendre ce qui définit in vivo la transmission du prion. Mon modèle d étude est la protéine Ure2p propageant le prion [URE3] dans la levure S. cerevisiae. J ai montré que la concentration cellulaire d Ure2p détermine la vitesse d agrégation de la protéine prion et donc son efficacité de transmission. En effet, de trop fortes concentrations cellulaires sont incompatibles avec la propagation du prion. La concentration cellulaire d Ure2p définit également la diversité des souches prions. Un crible génétique m a permit de mettre en évidence que la présence de séquences centromériques surnuméraires dans la cellule interfère avec la transmission du prion [URE3]. Le même phénomène est observé avec une augmentation du niveau de ploïdie de la cellule. Dans les deux cas, la surexpression du chaperon Hsp104 restaure une propagation normale du prion.A prion protein can adopt two distinct conformations, one cellular and one prion. Prion conformation is the result of its aggregation into amyloid fibers. This fiber is the support of the prion information from which the cellular isoforms are converted into prion form by autocatalytic manner. The prion information transmission is therefore based on the transmission of this fiber during cell division, which is done by small polymers. These are the result of a balance between fragmentation and polymerization of the fiber. A disturbance of this balance causes a massive aggregation of the prion protein, leading to the prion information loss.The objective of my thesis was to understand what defined in vivo the prion transmission. My studying model was the Ure2p protein propagating the [URE3] prion in S. cerevisiae yeast. I showed that the Ure2p cellular concentration determined the aggregation speed of the prion protein and thus its transmission efficiency. Indeed, too high cellular concentrations are incompatible with the prion propagation. The cellular concentration of Ure2p also defines the prion strains diversity. A genetic screen allowed me to highlight that the presence of centrometric supernumerary sequences in the cell interferes with the [URE3] prion transmission. The same phenomenon is observed with an increase in the cell ploidy. In both cases, overexpression of the Hsp104 chaperone restores normal prion propagation.BORDEAUX2-Bib. électronique (335229905) / SudocSudocFranceF