Mediterranean Long Shelf-Life Landraces: An Untapped Genetic Resource for Tomato Improvement

[EN] The Mediterranean long shelf-life (LSL) tomatoes are a group of landraces with a fruit remaining sound up to 6¿12 months after harvest. Most have been selected under semi-arid Mediterranean summer conditions with poor irrigation or rain-fed and thus, are drought tolerant. Besides the convergence in the latter traits, local selection criteria have been very variable, leading to a wide variation in fruit morphology and quality traits. The different soil characteristics and agricultural management techniques across the Mediterranean denote also a wide range of plant adaptive traits to different conditions. Despite the notorious traits for fruit quality and environment adaptation, the LSL landraces have been poorly exploited in tomato breeding programs, which rely basically on wild tomato species. In this review, we describe most of the information currently available for Mediterranean LSL landraces in order to highlight the importance of this genetic resource. We focus on the origin and diversity, the main selective traits, and the determinants of the extended fruit shelf-life and the drought tolerance. Altogether, the Mediterranean LSL landraces are a very valuable heritage to be revalued, since constitutes an alternative source to improve fruit quality and shelf-life in tomato, and to breed for more resilient cultivars under the predicted climate change conditions.This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 727929 (TOMRES), No 634561 (TRADITOM) and No 679796 (TomGEM). Research has been also supported by the Spanish Ministry of Economy and Competitiveness (MINECO) project AGL2013-42364-R (TOMDRO), and the Government of the Balearic Islands grants BIA20/07, BIA07/08, BIA09/12 and AAEE56/2015. 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The additional singleton task has become a popular paradigm to explore visual statistical learning and selective attention. In this task, participants are instructed to find a different-shaped target among a series of distractors as fast as possible. In some trials, the search display includes a singleton distractor with a different color, making search more difficult. This singleton distractor appears more often in one location than in the remaining locations. The typical results of these experiments show that participants learn to ignore the area of the screen that is more likely to contain the singleton distractor. It is often claimed that this learning takes place unconsciously, because at the end of the experiment participants seem to be unable to identify the location where the singleton distractor appeared most frequently during the task. In the present study, we tested participants’ awareness in three high-powered experiments using alternative measures. Contrary to previous studies, the results show clear evidence of explicit knowledge about which area of the display was more likely to contain the singleton distractor, suggesting that this type of learning might not be unconsciousOpen Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was supported by grants 2016-T1/SOC-1395 (MAV, TGF), and 2017-T1/SOC-5147 (DL, TGF) and 2020-5A/SOC-19723 (MAV) from Comunidad de Madrid (Programa de Atracción de Talento Investigador) and by grants PSI2017-85159-P (MAV, FVC), PGC2018-094694-B-I00 (DL) and PID2020-118583GB-I00 (MAV, TGF, FVC) from Agencia Estatal de Investigación and FEDER, UE. FVC was supported by PhD fellowship PRE2018-085148 from Ministerio de Ciencia e Innovació

Taxing Capital? Not a Bad Idea After All!

Premi a l'excel·lència investigadora. 2010Publicat també com a : CEPR Discussion Paper - ISSN 0265-8003 Núm. 5929 (2006), p. 1-55We quantitatively characterize the optimal capital and labor income tax in an overlapping generations model with idiosyncratic, uninsurable income shocks and permanent productivity differences of households. The optimal capital income tax rate is significantly positive at 36 percent. The optimal progressive labor income tax is, roughly, a flat tax of 23 percent with a deduction of #7,200 (relative to average household income of #42,000). The high optimal capital income tax is mainly driven by the life-cycle structure of the model, whereas the optimal progressivity of the labor income tax is attributable to the insurance and redistribution role of the tax system. (JEL E13, H21, H24, H25

Contribución de la Red de control de la calidad biológica de las aguas super ciales de Navarra al conocimiento distributivo de los odonatos fluviales de Navarra

Simposio Ibérico de Odonatología (2º. 2018. Lugo)El Servicio del Agua del Gobierno de Navarra cuenta con una amplia red de muestreo para el estudio anual de los Índices Bióticos de los ríos de Navarra. Entre los parámetros analizados se encuentran los macroinvertebrados bentónicos, grupo faunístico que incluye a las larvas de odonatos. La metodología utilizada se basa en las directrices emanadas de la propuesta AQEM (Assessment System for the Ecological Quality of Streams and Rivers throughout Europe using Benthic Macroinvertebrates) para la implementación de la Directiva Marco del Agua y consiste en la toma de muestras, %jación y procesamiento en laboratorio, donde tiene lugar la identi%cación taxonómica de los organismos bentónicos, hasta el nivel de Familia en el caso de los odonatos. Dado que los odonatos constituyen el orden de invertebrados que comparativamente a su número de especies tienen una mayor presencia en catálogos y normas sobre especies amenazadas (Directiva 92/43/CEE de Hábitats, Lista Roja de los invertebrados de España, etc.), y que el conocimiento sobre este grupo faunístico en el territorio navarro se reduce mayoritariamente a recopilaciones de observaciones y citas de imagos, se han extraído las larvas de odonatos contenidas en las muestras recolectadas en la red fluvial de Navarra los últimos 7 años, identificándolas hasta el nivel taxonómico más bajo posible. Así, han podido identificarse 678 ejemplares pertenecientes a 20 taxones distintos, entre los que destacan Coenagrion mercuriale (Charpentier, 1840), Oxygastra curtisii (Dale, 1834), Gomphus similimus (Sélys, 1840) y Gomphus vulgatissimus (Linnaeus, 1758). Se aportan mapas con las localizaciones, en muchos casos nuevas, para estas especies

Plasmodium knowlesi Genome Sequences from Clinical Isolates Reveal Extensive Genomic Dimorphism.

Plasmodium knowlesi is a newly described zoonosis that causes malaria in the human population that can be severe and fatal. The study of P. knowlesi parasites from human clinical isolates is relatively new and, in order to obtain maximum information from patient sample collections, we explored the possibility of generating P. knowlesi genome sequences from archived clinical isolates. Our patient sample collection consisted of frozen whole blood samples that contained excessive human DNA contamination and, in that form, were not suitable for parasite genome sequencing. We developed a method to reduce the amount of human DNA in the thawed blood samples in preparation for high throughput parasite genome sequencing using Illumina HiSeq and MiSeq sequencing platforms. Seven of fifteen samples processed had sufficiently pure P. knowlesi DNA for whole genome sequencing. The reads were mapped to the P. knowlesi H strain reference genome and an average mapping of 90% was obtained. Genes with low coverage were removed leaving 4623 genes for subsequent analyses. Previously we identified a DNA sequence dimorphism on a small fragment of the P. knowlesi normocyte binding protein xa gene on chromosome 14. We used the genome data to assemble full-length Pknbpxa sequences and discovered that the dimorphism extended along the gene. An in-house algorithm was developed to detect SNP sites co-associating with the dimorphism. More than half of the P. knowlesi genome was dimorphic, involving genes on all chromosomes and suggesting that two distinct types of P. knowlesi infect the human population in Sarawak, Malaysian Borneo. We use P. knowlesi clinical samples to demonstrate that Plasmodium DNA from archived patient samples can produce high quality genome data. We show that analyses, of even small numbers of difficult clinical malaria isolates, can generate comprehensive genomic information that will improve our understanding of malaria parasite diversity and pathobiology

Biological basis of extensive pleiotropy between blood traits and cancer risk

Background: The immune system has a central role in preventing carcinogenesis. Alteration of systemic immune cell levels may increase cancer risk. However, the extent to which common genetic variation influences blood traits and cancer risk remains largely undetermined. Here, we identify pleiotropic variants and predict their underlying molecular and cellular alterations. Methods: Multivariate Cox regression was used to evaluate associations between blood traits and cancer diagnosis in cases in the UK Biobank. Shared genetic variants were identified from the summary statistics of the genome-wide association studies of 27 blood traits and 27 cancer types and subtypes, applying the conditional/conjunctional false-discovery rate approach. Analysis of genomic positions, expression quantitative trait loci, enhancers, regulatory marks, functionally defined gene sets, and bulk- and single-cell expression profiles predicted the biological impact of pleiotropic variants. Plasma small RNAs were sequenced to assess association with cancer diagnosis. Results: The study identified 4093 common genetic variants, involving 1248 gene loci, that contributed to blood-cancer pleiotropism. Genomic hotspots of pleiotropism include chromosomal regions 5p15-TERT and 6p21-HLA. Genes whose products are involved in regulating telomere length are found to be enriched in pleiotropic variants. Pleiotropic gene candidates are frequently linked to transcriptional programs that regulate hematopoiesis and define progenitor cell states of immune system development. Perturbation of the myeloid lineage is indicated by pleiotropic associations with defined master regulators and cell alterations. Eosinophil count is inversely associated with cancer risk. A high frequency of pleiotropic associations is also centered on the regulation of small noncoding Y-RNAs. Predicted pleiotropic Y-RNAs show specific regulatory marks and are overabundant in the normal tissue and blood of cancer patients. Analysis of plasma small RNAs in women who developed breast cancer indicates there is an overabundance of Y-RNA preceding neoplasm diagnosis. Conclusions: This study reveals extensive pleiotropism between blood traits and cancer risk. Pleiotropism is linked to factors and processes involved in hematopoietic development and immune system function, including components of the major histocompatibility complexes, and regulators of telomere length and myeloid lineage. Deregulation of Y-RNAs is also associated with pleiotropism. Overexpression of these elements might indicate increased cancer risk

Anti-IL-6 Receptor Tocilizumab in Refractory Graves? Orbitopathy: National Multicenter Observational Study of 48 Patients

Graves’ orbitopathy (GO) is the most common extrathyroidal manifestation of Graves’ disease (GD). Our aim was to assess the e cacy and safety of Tocilizumab (TCZ) in GO refractory to conventional therapy. This was an open-label multicenter study of glucocorticoid-resistant GO treated with TCZ. The main outcomes were the best-corrected visual acuity (BVCA), Clinical Activity Score (CAS) and intraocular pressure (IOP). These outcome variables were assessed at baseline, 1st, 3rd, 6th and 12th month after TCZ therapy onset. The severity of GO was assessed according to the European Group on Graves’ Orbitopathy (EUGOGO). We studied 48 (38 women and 10 men) patients (95 eyes); mean age standard deviation 51 11.8 years. Before TCZ and besides oral glucocorticoids, they had received IV methylprednisolone (n = 43), or selenium (n = 11). GO disease was moderate (n =29) or severe (n = 19) and dysthyroid optic neuropathy (DON) (n = 7). TCZ was used in monotherapy (n = 45) or combined (n = 3) at a dose of 8 mg/kg IV every four weeks (n = 43) or 162 mg/s.c. every week (n = 5). TCZ yielded a significant improvement in all of the main outcomes at the 1st month that was maintained at one year. Comparing the baseline with data at 1 year all of the variables improved; BCVA (0.78 0.25 vs. 0.9 0.16; p = 0.0001), CAS (4.64 1.5 vs. 1.05 1.27; p = 0.0001) and intraocular pressure (IOP) (19.05 4.1 vs. 16.73 3.4 mmHg; p = 0.007). After a mean follow-up of 16.1 2.1 months, low disease activity (CAS 3), was achieved in 88 eyes (92.6%) and TCZ was withdrawn in 29 cases due to low disease activity (n = 25) or ine cacy (n = 4). No serious adverse events were observed. In conclusion, TCZ is a useful and safe therapeutic option in refractory GO treatment.This work was also partially supported by RETICS Programs, RD08/0075 (RIER) and RD12/0009/0013 from “Instituto de Salud Carlos III” (ISCIII) (Spain)

Evaluación de la enseñanza a través de la guía docente en la UPCT

[SPA]Este trabajo presenta una propuesta de metodología para la evaluación de guías docentes basada en un conjunto de tres criterios principales (utilidad, coherencia e idoneidad), once subcriterios y una serie de indicadores cuyos valores se obtienen a través de cuestionarios dirigidos a centros, departamentos, estudiantes y profesorado. Los cuestionarios parten del modelo de Guía Docente adoptado por la UPCT y contemplan todos los apartados de la misma. El método propuesto puede aplicarse también para mejorar los programas de evaluación del profesorado, pues aporta las evidencias que se necesitan para valorar la labor de planificación desarrollada por el mismo, la cual se plasma en la guía docente. [ENG]This paper presents a methodological proposal for the evaluation of teaching guides based on three main criteria, i.e. usefulness, consistency, and suitability, and eleven subcriteria. It is also based on different indicators obtained through questionnaires aimed at Educational Centres, Departments, Students and Professors. These questionnnaires have been prepared from the teaching guide model adopted by the Technical University of Cartagena (UPCT), and considering all the sections included in that guide. The proposed methodology can be also used to improve the teaching staff evaluation program, because of the inclusion of planning task carried out by the professor, an aspect considered in the teaching guide.Campus Mare Nostrum, Universidad Politécnica de Cartagena, Universidad de Murcia, Región de Murcia

Analysis of the common genetic component of large-vessel vasculitides through a meta- Immunochip strategy

Giant cell arteritis (GCA) and Takayasu's arteritis (TAK) are major forms of large-vessel vasculitis (LVV) that share clinical features. To evaluate their genetic similarities, we analysed Immunochip genotyping data from 1,434 LVV patients and 3,814 unaffected controls. Genetic pleiotropy was also estimated. The HLA region harboured the main disease-specific associations. GCA was mostly associated with class II genes (HLA-DRB1/HLA-DQA1) whereas TAK was mostly associated with class I genes (HLA-B/MICA). Both the statistical significance and effect size of the HLA signals were considerably reduced in the cross-disease meta-analysis in comparison with the analysis of GCA and TAK separately. Consequently, no significant genetic correlation between these two diseases was observed when HLA variants were tested. Outside the HLA region, only one polymorphism located nearby the IL12B gene surpassed the study-wide significance threshold in the meta-analysis of the discovery datasets (rs755374, P?=?7.54E-07; ORGCA?=?1.19, ORTAK?=?1.50). This marker was confirmed as novel GCA risk factor using four additional cohorts (PGCA?=?5.52E-04, ORGCA?=?1.16). Taken together, our results provide evidence of strong genetic differences between GCA and TAK in the HLA. Outside this region, common susceptibility factors were suggested, especially within the IL12B locus