Evolution of Flavylium-Based Color Systems in Plants: What Physical Chemistry Can Tell Us

Anthocyanins are the basis of the color of angiosperms, 3-deoxyanthocyanins and sphagnorubin play the same role in mosses and ferns, and auronidins are responsible for the color in liverworts. In this study, the color system of cyanidin-3-O-glucoside (kuromanin) as a representative compound of simpler anthocyanins was fully characterized by stopped flow. This type of anthocyanin cannot confer significant color to plants without intra- or intermolecular interactions, complexation with metals or supramolecular structures as in Commelina communis. The anthocyanin's color system was compared with those of 3-deoxyanthocyanins and riccionidin A, the aglycone of auronidins. The three systems follow the same sequence of chemical reactions, but the respective thermodynamics and kinetics are dramatically different.info:eu-repo/semantics/publishedVersio

Too Many Is Too Bad: Long-Term Net Negative Effects of High Density Ungulate Populations on a Dominant Mediterranean Shrub

Plant–animal interactions imply costs and benefits with net balance depending on interacting species and ecological context. Ungulates, in particular, confer costs (e.g., plant leaf consumption, flower bud predation) and benefits (e.g., plant overcompensation, seed dispersal) to plants. Magnitude of costs and benefits may be altered by habitat management or ecological conditions favoring high density ungulate populations. Little is known however on whether plant costs or benefits predominate over the years, or the long-term outcomes of plant-animal interactions in habitat types sustaining high density ungulate populations. We investigated how high density ungulate populations alter plant costs and benefits by quantifying ungulate long-term effects on the shrub Cistus ladanifer (Cistaceae) individual size, seed weight and number, seed bank, and population density, through a 12-year ungulate exclusion experiment in a Mediterranean scrubland. We monitored plant size and flower buds in plants exposed or protected from ungulates and number of developed capsules and seeds consumed (potential seed dispersal) by ungulates during three reproductive seasons. We found that ungulates negatively affected shrub size and led to a dramatically decline of shrub reproductive structures and seed production, affecting the plant reproductive cycle. Number of buds was 27 times higher and number of developed seed 5 times higher in ungulate-excluded as compared to ungulate-exposed plots. After 9 years of ungulate exclusion, the C. ladanifer seed bank was 2.6 times higher in ungulate-excluded plots. The population density of C. ladanifer was 4 times higher in ungulate-excluded plots. Our long-term experiment showed that high density ungulate populations can alter plant-animal interactions by reducing plant benefits and increasing plant costs.Peer reviewe

Chemical characterization and bioactivity of phytochemicals from Iberian endemic Santolina semidentata and strategies for ex situ propagation

Asteraceae family members are well-known for their medicinal potential, comprising several properties that make them unique among plants. Here we focus on Santolina semidentata, an endemic plant from the Iberian Peninsula, not yet described for its medicinal properties. Phytochemical characterization of S. semidentata was performed, concerning total phenol content, flavonoid content, antioxidant capacity, HPLC-DAD profile, acetylcholinesterase inhibitory capacity, cytotoxicity and neuroprotective effect in a human neurodegeneration cell model. Moreover, essential oil composition and antifungal activity were also analised. This oil might be useful for therapeutical purposes, particularly in the treatment of dermatophytosis. S. semidentata potential for neuroprotection was revealed by acetylcholinesterase inhibitory capacity and also by an effective protective effect in human neuronal cells. Furthermore, different seed conservation protocols, as well as successful in vitro propagation were established which may be useful when integrated in a broad strategy for the conservation of these endemic plants and their sustainable use for potential biotechnological applications. The results presented here greatly contribute to value this species regarding its potential as a source of phytochemicals with prospective neuroprotective health benefits, either as alternative neuroprotective drugs or as leads for synthetizing more effective molecules.The authors wish to thank to “Fundo EDP para a Biodiversidade” for financial support. This work was also supported by “Fundação para a Ciência e a Tecnologia” through grant PEst-OE/EQB/LA0004/2011, BGCT/33418/2008, Green-it: UID/Multi/04551/2013, iNOVA4Health: UID/Multi/04462/2013 and financial support to CNS (IF/01097/20132), RP (SFRH/BD/63615/2009), IF (SFRH/BD/86584/2012) and AG (SFRH/BD/103155/2014).info:eu-repo/semantics/publishedVersio

Bottom-up cascading effects of quarry revegetation deplete bird-mediated seed dispersal services

Quarrying activities cause profound modifications on ecosystems, such as removal of vegetation cover, biodiversity loss and depletion of ecosystem services. Ecological restoration stands as a solution to revert such effects. Concomitantly, awareness is currently being given on ecosystem services and ecological processes to evaluate restoration efficiency. The objective of the study was to assess restoration success in a quarry subjected to restoration practices for the last 40 years involving the plantation of native Mediterranean vegetation and the non-native Aleppo pine Pinus halepensis. The study was carried out by assessing the effectiveness of seed dispersal service provided by birds in the restored quarry by comparing this service to neighbouring natural (shrubland) and other semi-natural areas (oak-pine mixed open and Aleppo pine forest) present at the landscape. For this purpose, we explored bird composition structure and seed dispersal networks using point counts and faecal samples of mist-netted birds. We also collected vegetation structure data and explored its effect on bird community composition. Our results showed that bird abundance in the restored quarry was significantly lower, and its bird community was compositionally different than natural shrubland and semi-natural areas. For instance, seed-dispersing birds, woody and shrub/ground foragers and partially migrators were the most affected groups at the restored area. Bird community composition and their traits were likely driven by vegetation characteristics, with higher native vegetation cover and fruit richness promoting higher bird abundance and Aleppo pine cover negatively influencing seed-dispersing birds. Concurrently, seed dispersal network in the restored quarry was less complex than in other areas. Seed dispersal services in the restored quarry were below the reported values for neighbouring natural and semi-natural areas and are likely driven by the low abundance of seed-dispersing birds. We consider that the causes affecting this group's low abundance can be related to revegetation measures favouring Aleppo pine, combined with a shallow soil depth and poor soil quality, which may have constrained native vegetation development. We conclude that seed dispersal services at the quarry are depleted, which may suggest a low restoration success concerning ecosystem functioning. Our results strengthen that quarry revegetation with non-native species must be avoided, since it alters bird community composition, and consequently, affects seed dispersal service provided by birds

Adapting Agriculture to Climate Change: A Synopsis of Coordinated National Crop Wild Relative Seed Collecting Programs across Five Continents

The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, and accessibility of germplasm collections of crop wild relatives (CWR). Between 2013 and 2018, partners in 25 countries, heirs to the globetrotting legacy of Nikolai Vavilov, undertook seed collecting expeditions targeting CWR of 28 crops of global significance for agriculture. Here, we describe the implementation of the 25 national collecting programs and present the key results. A total of 4587 unique seed samples from at least 355 CWR taxa were collected, conserved ex situ, safety duplicated in national and international genebanks, and made available through the Multilateral System (MLS) of the International Treaty on Plant Genetic Resources for Food and Agriculture (Plant Treaty). Collections of CWR were made for all 28 targeted crops. Potato and eggplant were the most collected genepools, although the greatest number of primary genepool collections were made for rice. Overall, alfalfa, Bambara groundnut, grass pea and wheat were the genepools for which targets were best achieved. Several of the newly collected samples have already been used in pre-breeding programs to adapt crops to future challenges.info:eu-repo/semantics/publishedVersio

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Ammonium as a driving force of plant diversity and ecosystem functioning: observations based on 5 Years' manipulation of N dose and form in a mediterranean ecosystem

Enhanced nitrogen (N) availability is one of the main drivers of biodiversity loss and degradation of ecosystem functions. However, in very nutrient-poor ecosystems, enhanced N input can, in the short-term, promote diversity. Mediterranean Basin ecosystems are nutrient-limited biodiversity hotspots, but no information is available on their medium- or long-term responses to enhanced N input. Since 2007, we have been manipulating the form and dose of available N in a Mediterranean Basin maquis in south-western Europe that has low ambient N deposition (,4 kg N ha21 yr21) and low soil N content (0.1%). N availability was modified by the addition of 40 kg N ha21 yr21 as a 1:1 NH4Cl to (NH4)2SO4 mixture, and 40 and 80 kg N ha21 yr21 as NH4NO3. Over the following 5 years, the impacts on plant composition and diversity (richness and evenness) and some ecosystem characteristics (soil extractable N and organic matter, aboveground biomass and % of bare soil) were assessed. Plant species richness increased with enhanced N input and was more related to ammonium than to nitrate. Exposure to 40 kg NH4 +-N ha21 yr21 (alone and with nitrate) enhanced plant richness, but did not increase aboveground biomass; soil extractable N even increased under 80 kg NH4NO3-N ha21 yr21 and the % of bare soil increased under 40 kg NH4 +-N ha21 yr21. The treatment containing less ammonium, 40 kg NH4NO3-N ha21 yr21, did not enhance plant diversity but promoted aboveground biomass and reduced the % of bare soil. Data suggest that enhanced NHy availability affects the structure of the maquis, which may promote soil erosion and N leakage, whereas enhanced NOx availability leads to biomass accumulation which may increase the fire risk. These observations are relevant for land use management in biodiverse and fragmented ecosystems such as the maquis, especially in conservation areas

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Weld (Reseda luteola) was one of the main sources of yellow dyes used for dyeing textiles and to prepare artists’ pigments in Europe until the 19th century. For the first time, this work explores the technology of preparing weld lake pigments in the 19th century by Winsor & Newton (W&N), a renowned supplier of artists’ materials. Five recipes were discovered in the W&N 19th century Archive Database and reconstructed in the laboratory. W&N was extracting weld in neutral and basic media, and preparing the insoluble lake by complexation with Al3+ in the form of alum (KAl(SO4)2•12H2O) or hydrated alumina (Al(OH)3). Five yellow lake pigments were successfully obtained and characterized by High-Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) and Fourier Transform Infrared Spectroscopy (FTIR). Their chromatographic profiles display as main yellows, luteolin 7-O-glucoside (Lut-7-O-glu) or both Lut-7-O-glu plus luteolin 3′,7-O-glucoside (Lut-3’,7-O-glu). In two of the processes, the presence of gypsum (CaSO4•2H2O) was unequivocally detected by FTIR, being formed as a by-product. This work offers the first identification of weld lake pigments’ characteristic infrared bands. The W&N Database proved again to be a unique source of information on 19th-century artists’ materials and their commercial preparation. The knowledge gain is essential to ensure effective conservation and authentication procedures.info:eu-repo/semantics/publishedVersio

Short-term seed storage of two Mediterranean shrubs used in restoration: Simple procedures to reduce seed deterioration

Seed-based revegetation is fundamental in global restoration efforts. When seed storage is mandatory, knowledge of storage conditions that reduce ageing is essential for effective conservation of viable seeds. However, inappropriate storage is still frequent, particularly in local restoration projects involving native species. We investigated how different storage conditions (temperature and relative humidity) affected seed viability of the Mediterranean shrubs Arbutus unedo and Myrtus communis. We aimed to identify which conditions reduced seed deterioration and to provide practitioners with practical storage guidelines to overcome the natural fluctuations in seed production of these two species. Seeds equilibrated at 15%, 30% and 60% relative humidity (RH) and sealed in airtight containers were stored at 5 °C or ambient temperature over one year. Additional seed lots were kept under ambient conditions, according to the usual local practice (open storage). Germination tests were performed prior to and after storage. Significant seed deterioration (80–100%) occurred in seeds equilibrated at 60% RH and stored at ambient temperature, and in seeds kept in open storage. The remaining treatments maintained high seed viability. Short seed longevity under ambient conditions was demonstrated for both species. To store seeds over one year, practitioners may either (i) equilibrate seeds up to 60% RH and store them in a refrigerator (5 °C), or (ii) equilibrate seeds up to 30% RH (preferably 15% RH for A. unedo) and store them at ambient temperature. All seeds should be sealed into airtight containers immediately after equilibrium. The effectiveness of the 5 °C-60% RH treatment demonstrated that procedures less demanding than international standards for short-term storage can still ensure seed viability. While contributing to the understanding of species responses to storage conditions, our findings can be applied to both species by local practitioners across the Mediterranean. Moreover, our approach can be adapted and extended to other restoration projects and native species, supporting specific improvements to seed storage protocols, thereby enhancing the cost-effectiveness and the capacity of restoration efforts.info:eu-repo/semantics/publishedVersio