Family Meanings, Contexts and Hopes Within a Clinical Trial

These qualitative analyses are a component of the clinical trial: “Effects of Sertraline Treatment for Young Children with FXS: Examination of Developmental Outcomes and Family Contexts.” We argue that inclusion of a sociocultural perspective within the context of a clinical trial affords consideration of: A dynamic view of development – simultaneous consideration of biological and sociocultural factors (Vygotsky, 1978). Application of a bioecological framework and consideration of ever changing nested settings and contexts over time (Bronfenbrenner, 2005

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

The genetic architecture of the human cerebral cortex

The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder

Genomic investigations of unexplained acute hepatitis in children

Since its first identification in Scotland, over 1,000 cases of unexplained paediatric hepatitis in children have been reported worldwide, including 278 cases in the UK1. Here we report an investigation of 38 cases, 66 age-matched immunocompetent controls and 21 immunocompromised comparator participants, using a combination of genomic, transcriptomic, proteomic and immunohistochemical methods. We detected high levels of adeno-associated virus 2 (AAV2) DNA in the liver, blood, plasma or stool from 27 of 28 cases. We found low levels of adenovirus (HAdV) and human herpesvirus 6B (HHV-6B) in 23 of 31 and 16 of 23, respectively, of the cases tested. By contrast, AAV2 was infrequently detected and at low titre in the blood or the liver from control children with HAdV, even when profoundly immunosuppressed. AAV2, HAdV and HHV-6 phylogeny excluded the emergence of novel strains in cases. Histological analyses of explanted livers showed enrichment for T cells and B lineage cells. Proteomic comparison of liver tissue from cases and healthy controls identified increased expression of HLA class 2, immunoglobulin variable regions and complement proteins. HAdV and AAV2 proteins were not detected in the livers. Instead, we identified AAV2 DNA complexes reflecting both HAdV-mediated and HHV-6B-mediated replication. We hypothesize that high levels of abnormal AAV2 replication products aided by HAdV and, in severe cases, HHV-6B may have triggered immune-mediated hepatic disease in genetically and immunologically predisposed children

“Find Ways to Cope:” Games and Gamification Supporting College Student Mental Health During the Pandemic

The COVID- 19 pandemic has created challenges for student mental health in higher education. Emerging literature documents the various challenges that today’s college students encounter, but students’ coping strategies are understudied. Our work examines how college students utilize gaming as a tool to transition into a new campus environment and address mental health. Using a grounded theory approach, our analysis of interviews with freshmen on UC Davis campus during Fall 2021 revealed that college students use mental health apps and games in response to pandemic-related stress and anxiety. Students also articulated connecting to their peers as well as to themselves. We argue that gaming plays a critical role in this historic time by supporting students in their pandemic-lives on a college campus under stressful circumstances

“Seeing power” between young people and conservation professionals in the design of a community-based watershed monitoring initiative

Background: Community-based monitoring provides a forum for diverse stakeholders to co-construct knowledge relevant to building social-ecological resilience. However, power asymmetries between these actors can privilege the perspectives of dominant groups, while preventing non-dominant perspectives from informing conservation science. Methods: This study investigates a workshop series intended to support young people in designing a watershed monitoring initiative rooted in their own interests with respect to a large dam removal in their community. We use interaction analysis to examine whose ideas are taken up in discussions among young people, educators, conservation professionals, and education researchers. Findings: Power dynamics that privilege the contributions of credentialed professionals over those of young people can constrain collective learning processes while simultaneously generating tensions that allow for expansive learning to occur. Facilitation practices and other pedagogical moves play an important role in either further entrenching or disrupting hierarchies between youth and community partners. Contribution: Our analysis reveals how careful attention to interactional dynamics—both as a research method and as a pedagogical practice—can make visible and disrupt epistemic hierarchies in multi-stakeholder learning environments. Problematizing these hierarchies can help broaden the perspectives from which knowledge is generated, a necessary endeavor in building resilient social-ecological systems

Becoming Reflective: Designing for Reflection on Physical Performances

Learners’ physical performances can serve as focal objects for reflection and insight across a variety of contexts and content areas. This session brings together a set of projects that leverage the physical performances of learners, construct concrete and abstract representations of those performances, and investigate how learners reflect on and understand the relationships between their performances and target content—physics, health and fitness, data literacy and navigation, animal foraging, and climate change. The session will share findings and design principles from each of the studies around constructing technological scaffolds for physical performance reflections. The symposium highlights the various ways performance can be used to engage learners, and how different settings and learning goals affect the designs of performance representations