Historical Building Monitoring Using an Energy-Efficient Scalable Wireless Sensor Network Architecture

We present a set of novel low power wireless sensor nodes designed for monitoring wooden masterpieces and historical buildings, in order to perform an early detection of pests. Although our previous star-based system configuration has been in operation for more than 13 years, it does not scale well for sensorization of large buildings or when deploying hundreds of nodes. In this paper we demonstrate the feasibility of a cluster-based dynamic-tree hierarchical Wireless Sensor Network (WSN) architecture where realistic assumptions of radio frequency data transmission are applied to cluster construction, and a mix of heterogeneous nodes are used to minimize economic cost of the whole system and maximize power saving of the leaf nodes. Simulation results show that the specialization of a fraction of the nodes by providing better antennas and some energy harvesting techniques can dramatically extend the life of the entire WSN and reduce the cost of the whole system. A demonstration of the proposed architecture with a new routing protocol and applied to termite pest detection has been implemented on a set of new nodes and should last for about 10 years, but it provides better scalability, reliability and deployment properties

An RxLR effector from phytophthora infestans prevents re-localisation of two plant NAC transcription factors from the endoplasmic reticulum to the nucleus

The plant immune system is activated following the perception of exposed, essential and invariant microbial molecules that are recognised as non-self. A major component of plant immunity is the transcriptional induction of genes involved in a wide array of defence responses. In turn, adapted pathogens deliver effector proteins that act either inside or outside plant cells to manipulate host processes, often through their direct action on plant protein targets. To date, few effectors have been shown to directly manipulate transcriptional regulators of plant defence. Moreover, little is known generally about the modes of action of effectors from filamentous (fungal and oomycete) plant pathogens. We describe an effector, called Pi03192, from the late blight pathogen Phytophthora infestans, which interacts with a pair of host transcription factors at the endoplasmic reticulum (ER) inside plant cells. We show that these transcription factors are released from the ER to enter the nucleus, following pathogen perception, and are important in restricting disease. Pi03192 prevents the plant transcription factors from accumulating in the host nucleus, revealing a novel means of enhancing host susceptibility

Dissemination of Cephalosporin Resistance Genes between Escherichia coli Strains from Farm Animals and Humans by Specific Plasmid Lineages

Third-generation cephalosporins are a class of β-lactam antibiotics that are often used for the treatment of human infections caused by Gram-negative bacteria, especially Escherichia coli. Worryingly, the incidence of human infections caused by third-generation cephalosporin-resistant E. coli is increasing worldwide. Recent studies have suggested that these E. coli strains, and their antibiotic resistance genes, can spread from food-producing animals, via the food-chain, to humans. However, these studies used traditional typing methods, which may not have provided sufficient resolution to reliably assess the relatedness of these strains. We therefore used whole-genome sequencing (WGS) to study the relatedness of cephalosporin-resistant E. coli from humans, chicken meat, poultry and pigs. One strain collection included pairs of human and poultry-associated strains that had previously been considered to be identical based on Multi-Locus Sequence Typing, plasmid typing and antibiotic resistance gene sequencing. The second collection included isolates from farmers and their pigs. WGS analysis revealed considerable heterogeneity between human and poultry-associated isolates. The most closely related pairs of strains from both sources carried 1263 Single-Nucleotide Polymorphisms (SNPs) per Mbp core genome. In contrast, epidemiologically linked strains from humans and pigs differed by only 1.8 SNPs per Mbp core genome. WGS-based plasmid reconstructions revealed three distinct plasmid lineages (IncI1- and IncK-type) that carried cephalosporin resistance genes of the Extended-Spectrum Beta-Lactamase (ESBL)- and AmpC-types. The plasmid backbones within each lineage were virtually identical and were shared by genetically unrelated human and animal isolates. Plasmid reconstructions from short-read sequencing data were validated by long-read DNA sequencing for two strains. Our findings failed to demonstrate evidence for recent clonal transmission of cephalosporin-resistant E. coli strains from poultry to humans, as has been suggested based on traditional, low-resolution typing methods. Instead, our data suggest that cephalosporin resistance genes are mainly disseminated in animals and humans via distinct plasmids

Psychosocial impact of undergoing prostate cancer screening for men with BRCA1 or BRCA2 mutations.

OBJECTIVES: To report the baseline results of a longitudinal psychosocial study that forms part of the IMPACT study, a multi-national investigation of targeted prostate cancer (PCa) screening among men with a known pathogenic germline mutation in the BRCA1 or BRCA2 genes. PARTICPANTS AND METHODS: Men enrolled in the IMPACT study were invited to complete a questionnaire at collaborating sites prior to each annual screening visit. The questionnaire included sociodemographic characteristics and the following measures: the Hospital Anxiety and Depression Scale (HADS), Impact of Event Scale (IES), 36-item short-form health survey (SF-36), Memorial Anxiety Scale for Prostate Cancer, Cancer Worry Scale-Revised, risk perception and knowledge. The results of the baseline questionnaire are presented. RESULTS: A total of 432 men completed questionnaires: 98 and 160 had mutations in BRCA1 and BRCA2 genes, respectively, and 174 were controls (familial mutation negative). Participants' perception of PCa risk was influenced by genetic status. Knowledge levels were high and unrelated to genetic status. Mean scores for the HADS and SF-36 were within reported general population norms and mean IES scores were within normal range. IES mean intrusion and avoidance scores were significantly higher in BRCA1/BRCA2 carriers than in controls and were higher in men with increased PCa risk perception. At the multivariate level, risk perception contributed more significantly to variance in IES scores than genetic status. CONCLUSION: This is the first study to report the psychosocial profile of men with BRCA1/BRCA2 mutations undergoing PCa screening. No clinically concerning levels of general or cancer-specific distress or poor quality of life were detected in the cohort as a whole. A small subset of participants reported higher levels of distress, suggesting the need for healthcare professionals offering PCa screening to identify these risk factors and offer additional information and support to men seeking PCa screening

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Algunos aspectos de la estructura social y patrones de migración de las Yubartas o ballenas jorobadas, megaptera novaeangliae (borowsky, 1781), de la Isla de Gorgona, Pacífico colombiano

IP 6282-09-001-9

Migratory round-trip of individually identified humpback whales at the Strait of Magellan: clues on transit times and phylopatry to destinations Ciclo migratorio de ballenas jorobadas individualizadas del estrecho de Magallanes: indicios sobre la duración de la migración y filopatría en los destinos

Humpback whales undertake seasonal migration between productive high-latitude areas where they feed in summer and low-latitude tropical waters where mating and calving occur during winter. In the eastern south Pacific, the species breeds off Colombia and Ecuador, and feeds primarily in the western Antarctic Peninsula and in the waters of the Strait of Magellan (SM), recently described as a new feeding ground for humpback whales. Comparison of fluke photographs of 62 individuals from the SM obtained during the austral summer from 1999 to 2005 and 1,042 individuals from Colombia, provided conclusive matches for six individuals, with an overall interchange Índex of 0.093. Eight migratory trips between summer and winter grounds were registered for four whales during a complete migratory round-trip in consecutive years. The mínimum distance traveled in a one-way trip ranged from 6,650 to 7,000 km. The duration of the two fastest trips between these migratory destinations was 88 and 99 days, with a mean speed of migration of 76 and 67 km day-1 respectively. Five of the whales present in both areas were males and three mitochondrial DNA haplotypes were identified: EM-1 for three individuals, EM-2 for two and EM-3 for the last one, all of which have been previously described for humpback whales from Colombia. All six individuals were seen several days in each season in the SM (as many as 39 days in one case), with an average stay of 72 ± 40 days (n = 20) per year, ranging from 3 to 125 days. On average, each of the six individuals was seen in the SM during 71 ± 18 % of the seven monitored summers. Three individuals were re-sighted in the SM six out of the seven surveyed years, during four to six consecutive years. These results provide the first direct evidence to include humpback whales that feed in the Strait of Magellan as part of the eastern south Pacific population of whales that feed off Colombian waters.La ballena jorobada migra estacionalmente entre latitudes altas donde se alimenta en verano y latitudes bajas donde cría y se aparea en invierno. En el Pacífico sureste, la especie se reproduce en Colombia y Ecuador y se alimenta principalmente al oeste de la península Antartica, y en el estrecho de Magallanes (EM) recientemente descrito como nueva área de alimentación. Al comparar las fotografías de las colas de 62 ballenas individualizadas en el EM durante el verano austral entre 1999 y 2005 con 1.042 individuos de Colombia, se encontró a seis individuos comunes, lo que representa un índice de Intercambio migratorio de 0,093. Se registraron ocho migraciones para cuatro de estas ballenas entre el EM y Colombia en el ciclo migratorio de años consecutivos. La distancia mínima recorrida en una sola dirección varió entre 6.650 y 7.000 km. La duración de los dos viajes más rápidos registrados entre estos dos destinos fue de 88 y 99 días, con una velocidad promedio de migración de 76 y 67 km día-1 respectivamente. Cinco de las seis ballenas comunes entre las areas fueron machos. Entre las seis ballenas se encontraron tres haplotipos de ADN mitocondrial todos descritos previamente en ballenas jorobadas de Colombia: tres ballenas con el haplotipo EM-1, dos con el EM-2 y uno con el EM-3. Los seis individuos se avistaron reiteradamente en el EM (hasta 39 días en una estación en un caso), con una permanencia promedio de 72 ± 40 días (n = 20) por año y un rango entre 3 y 125 días. En promedio, estas seis ballenas se vieron durante el 71 ± 18 % de las siete temporadas muestreadas en el EM y tres se registraron seis de lo siete años estudiados, por 4-6 años consecutivos. Esta es la primera evidencia directa para incluir las ballenas jorobadas que se alimentan en el estrecho de Magallanes como parte de la población del Pacífico sureste que se reproduce en aguas colombianas

Effects of plasma rich in growth factors (PRGF) on biomechanicalproperties of Achilles tendon repair

[EN] To assess the biomechanical effects of intra-tendinous injections of PRGF on the healing Achilles tendon after repair in a sheep model. Thirty sheep were randomly assigned into one of the six groups depending on the type of treatment received (PRGF or placebo) and survival time (2, 4 and 8 weeks). The Achilles tendon injury was repaired by suturing the tendinous edges employing a three-loop pulley pattern. A trans-articular external fixation system was then used for immobilization. The PRGF or placebo was administered on a weekly basis completing a maximum of three infiltrations. The force, section and tension values were compared between the operated and healthy Achilles tendons across all groups. The PRGF-treated tendons had higher force at 8 weeks compared with the placebo group (p = 0.007). Between 2 and 4 weeks, a significant increase in force in both the PRGF-treated tendon (p = 0.0027) and placebo group (p = 0.0095) occurred. No significant differences were found for section ratio between PRGF-treated tendons and the placebo group for any of the time periods evaluated. At 2 weeks, PRGF-treated tendons had higher tension ratio compared with placebo group tendons (p = 0.0143). Both PRGF and placebo treatments significantly improved the force (p < 0.001 and p = 0.0095, respectively) and tension (p = 0.009 and p = 0.0039, respectively) ratios at 8 weeks compared with 2 weeks. The application of PRGF increases Achilles tendon repair strength at 8 weeks compared with the use of placebo. The use of PRGF does not modify section and tension ratios compared with placebo at 8 weeks. The tension ratio progressively increases between 2 and 8 weeks compared with the placebo.López-Nájera, D.; Rubio-Zaragoza, M.; Sopena-Juncosa, JJ.; Alentorn-Geli, E.; Cugat-Bertomeu, R.; Domínguez-Pérez, JM.; García-Balletbó, M.... (2016). Effects of plasma rich in growth factors (PRGF) on biomechanicalproperties of Achilles tendon repair. Knee Surgery, Sports Traumatology, Arthroscopy. 24(12):3997-4004. doi:10.1007/s00167-015-3725-2S399740042412Ajaxon I, Persson C (2014) Compressive fatigue properties of a commercially available acrylic bone cement for vertebroplasty. Biomech Model Mechanobiol 13(6):1199–1207Andia I, Sanchez M, Maffulli N (2010) Tendon healing and platelet-rich plasma therapies. Expert Opin Biol Ther 10(10):1415–1426Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT (2004) Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost 91(1):4–15Anitua E, Sanchez M, Nurden AT, Zalduendo M, de la Fuente M, Orive G, Azofra J, Andia I (2006) Autologous fibrin matrices: a potential source of biological mediators that modulate tendon cell activities. J Biomed Mater Res A 77(2):285–293Anitua E, Sanchez M, Orive G, Andia I (2007) The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials 28(31):4551–4560Anitua E, Sanchez M, Orive G, Andia I (2009) Shedding light in the controversial terminology for platelet rich products. J Biomed Mater Res A 90(4):1262–1263Aspenberg P, Virchenko O (2004) Platelet concentrate injection improves Achilles tendon repair in rats. Acta Orthop Scand 75(1):93–99Baums MH, Buchhorn GH, Spahn G, Poppendieck B, Schultz W, Klinger HM (2008) Biomechanical characteristics of single-row repair in comparison to double-row repair with consideration of the suture configuration and suture material. Knee Surg Sports Traumatol Arthrosc 16(11):1052–1060Beck J, Evans D, Tonino PM, Yong S, Callaci JJ (2012) The biomechanical and histologic effects of platelet-rich plasma on rat rotator cuff repairs. Am J Sports Med 40(9):2037–2044Bennett NT, Schultz GS (1993) Growth factors and wound healing: biochemical properties of growth factors and their receptors. Am J Surg 165(6):728–737Bowser JE, Elder SH, Rashmir-Raven AM, Swiderski CE (2011) A cryogenic clamping technique that facilitates ultimate tensile strength determinations in tendons and ligaments. Vet Comp Orthop Traumatol 24(5):370–373Bruce WJ, Frame K, Burbidge HM, Thompson K, Firth EC (2002) A comparison of the effects of joint immobilisation, twice-daily passive motion and voluntary motion on articular cartilage healing in sheep. Vet Comp Orthop Traumatol 15:23–29Fenwick SA, Hazleman BL, Riley GP (2002) The vasculature and its role in the damaged and healing tendon. Arthritis Res 4(4):252–260Fernandez-Sarmiento JA, Dominguez JM, Granados MM, Morgaz J, Navarrete R, Carrillo JM, Gomez-Villamandos RJ, Munoz-Rascon P, de Las Martin, Mulas J, Millan Y, Garcia-Balletbo M, Cugat R (2013) Histological study of the influence of plasma rich in growth factors (PRGF) on the healing of divided Achilles tendons in sheep. J Bone Joint Surg Am 95(3):246–255Hettrich CM, Gasinu S, Beamer BS, Stasiak M, Fox A, Birmingham P, Ying O, Deng XH, Rodeo SA (2014) The effect of mechanical load on tendon-to-bone healing in a rat model. Am J Sports Med 42(5):1233–1241Jarvinen TA, Kannus P, Maffulli N, Khan KM (2005) Achilles tendon disorders: etiology and epidemiology. Foot Ankle Clin 10(2):255–266Kaux JF, Drion PV, Colige A, Pascon F, Libertiaux V, Hoffmann A, Janssen L, Heyers A, Nusgens BV, Le Goff C, Gothot A, Cescotto S, Defraigne JO, Rickert M, Crielaard JM (2012) Effects of platelet-rich plasma (PRP) on the healing of Achilles tendons of rats. Wound Repair Regen 20(5):748–756Kim HJ, Nam HW, Hur CY, Park M, Yang HS, Kim BS, Park JH (2011) The effect of platelet rich plasma from bone marrow aspirate with added bone morphogenetic protein-2 on the Achilles tendon-bone junction in rabbits. Clin Orthop Surg 3(4):325–331Kim MY, Farnebo S, Woon CY, Schmitt T, Pham H, Chang J (2014) Augmentation of tendon healing with an injectable tendon hydrogel in a rat Achilles tendon model. Plast Reconstr Surg 133(5):645e–653eMaganaris C, Narici M, Almekinders L, Mafulli N (2007) Biomechanics of the Achilles tendon. In: Mafulli N, Almekinders L (eds) The Achilles tendon. Springer, London, pp 17–24Manning DW, Spiguel AR, Mass DP (2010) Biomechanical analysis of partial flexor tendon lacerations in zone II of human cadavers. J Hand Surg 35(1):11–18Martini L, Fini M, Giavaresi G, Giardino R (2001) Sheep model in orthopedic research: a literature review. Comp Med 51(4):292–299Monto RR (2012) Platelet rich plasma treatment for chronic Achilles tendinosis. Foot Ankle Int 33(5):379–385Musani S, Musani I, Dugal R, Habbu N, Madanshetty P, Virani D (2013) An in vitro comparative evaluation of micro tensile bond strength of two metal bonding resin cements bonded to cobalt chromium alloy. J Int Oral Health 5(5):73–78O’Brien M (2005) The anatomy of the Achilles tendon. Foot Ankle Clin 10(2):225–238Pearce AI, Richards RG, Milz S, Schneider E, Pearce SG (2007) Animal models for implant biomaterial research in bone: a review. Eur Cell Mater 13:1–10Sammarco GJ, Burstein AH, Davis WL, Frankel VH (1971) The biomechanics of torsional fractures: the effect of loading on ultimate properties. J Biomech 4(2):113–117Skerry TM, Lanyon LE (1993) Immobilisation induced bone loss in the sheep is not modulated by calcitonin treatment. Bone 14(3):511–516Solchaga LA, Bendele A, Shah V, Snel LB, Kestler HK, Dines JS, Hee CK (2014) Comparison of the effect of intra-tendon applications of recombinant human platelet-derived growth factor-BB, platelet-rich plasma, steroids in a rat achilles tendon collagenase model. J Orthop Res 32(1):145–150Uysal CA, Tobita M, Hyakusoku H, Mizuno H (2012) Adipose-derived stem cells enhance primary tendon repair: biomechanical and immunohistochemical evaluation. J Plast Reconstr Aesthet Surg 65(12):1712–1719Wang JH, Guo Q, Li B (2012) Tendon biomechanics and mechanobiology—a minireview of basic concepts and recent advancements. J Hand Ther 25(2):133–140 (quiz 141)Wnuk T, Blacha J, Mazurkiewicz T, Olchowik G, Chyzynska M (2012) The mechanical and histological estimation of calcaneal tendon callus in rats after PRP injection. Pol Orthop Traumatol 77:5–