Einstein on the beach: A study in temporality

This is an Author's Accepted Manuscript of an article published in Performance Research, 17(5), 34 - 40, 2012, copyright @ Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/13528165.2012.728438.In this paper I seek to examine and analyse the sense of duration induced by performances of Einstein on the Beach, and the entailed sense of time which its internal structure creates. I initially sketch out the stylistic context and artistic intentions of this work's creators, Glass and Wilson, and I briefly describe the process of its creation. Certain features of this process indicate how the work may be interpreted. Having cited the creators' thoughts on structure and temporality, I address directly aspects of Einstein's temporal effects, comparing it to works of similar lengths. I give the briefest synopsis of its staging and motifs. I then outline three kinds of devices which seem to inform our temporal sense of this work as spectators. In the final section I invoke two ideas which serve as analogies to help characterise this work's overall effect on us: Heidegger's notion of the ‘hermeneutic circle’ and, more speculatively, Nietzsche's ‘theory’ of Eternal Recurrence

Crystal structure, biochemical and biophysical characterisation of NHR1 domain of E3 Ubiquitin ligase neutralized

International audienceNotch signaling controls diverse developmental decisions of central importance to cell activity. One of the conserved positive regulators of No- tch signaling is Neuralized, the E3 Ubiquitin li-gase enzyme that regulates signaling activity by endocytosis. Neuralized has two novel repeats, NHR1 and NHR2, with a RING finger motif at the C-terminus. Both endocytosis of the Notch ligand, Delta, and inhibition of Notch signaling by Tom, a bearded family member, require the NHR1 domain. Here we describe the first crystal structure of NHR1 domain from Drosophila me- lanogaster, solved to 2.1 Å resolution by X-ray analysis. Using NMR and other biophysical tech- niques we define a minimal binding region of Tom, consisting of 12 residues, which interacts with NHR1 and show by interfacial analysis of protein monolayers that NHR1 binds PI4P. Taken together, the studies provide insight into mo-lecular interactions that are important for Notch signaling

Paper Session II-B - The Advanced Camera for the Hubble Space Telescope

The Hubble Space Telescope (HST) Advanced Camera for Surveys (ACS) will have a high throughput, wide field, optical and I-band camera (WFC), a critically sampled high resolution camera (HRC), and a high throughput, moderate field of view far ultraviolet, solar-blind camera (SBC). The key characteristics of the ACS are listed in Table 1. The throughputs include the geometrical, scattering, and reflectivity losses from the HST optical telescope assembly (Burrows, HST OTA Handbook). Two figures are listed for the ACS efficiencies. The first is the efficiency using the quantum efficiency (QE) of the Scientific Imaging Technologies (SITe) 2K x 4K WFC CCDs and the SITe HRC 1K ´ 1K CCDs selected for the first build of the flight cameras. The second and higher efficiencies are those achieved with SITe CCDs processed and anti-reflection coated at Steward Observatory by Dr. Michael Lesser. We plan to use these better CCDs for the second build of the flight cameras

Analysis of the natively unstructured RNA/protein-recognition core in the Escherichia coli RNA degradosome and its interactions with regulatory RNA/Hfq complexes.

The RNA degradosome is a multi-enzyme assembly that plays a central role in the RNA metabolism of Escherichia coli and numerous other bacterial species including pathogens. At the core of the assembly is the endoribonuclease RNase E, one of the largest E. coli proteins and also one that bears the greatest region predicted to be natively unstructured. This extensive unstructured region, situated in the C-terminal half of RNase E, is punctuated with conserved short linear motifs that recruit partner proteins, direct RNA interactions, and enable association with the cytoplasmic membrane. We have structurally characterized a subassembly of the degradosome-comprising a 248-residue segment of the natively unstructured part of RNase E, the DEAD-box helicase RhlB and the glycolytic enzyme enolase, and provide evidence that it serves as a flexible recognition centre that can co-recruit small regulatory RNA and the RNA chaperone Hfq. Our results support a model in which the degradosome captures substrates and regulatory RNAs through the recognition centre, facilitates pairing to cognate transcripts and presents the target to the ribonuclease active sites of the greater assembly for cooperative degradation or processing