Arsenite-Induced Alterations of DNA Photodamage Repair and Apoptosis After Solar-Simulation UVR in Mouse Keratinocytes in Vitro

Our laboratory has shown that arsenite markedly increased the cancer rate caused by solar-simulation ultraviolet radiation (UVR) in the hairless mouse skin model. In the present study, we investigated how arsenite affected DNA photodamage repair and apoptosis after solar-simulation UVR in the mouse keratinocyte cell line 291.03C. The keratinocytes were treated with different concentrations of sodium arsenite (0.0, 2.5, 5.0 μM) for 24 hr and then were immediately irradiated with a single dose of 0.30 kJ/m(2) UVR. At 24 hr after UVR, DNA photoproducts [cyclobutane pyrimidine dimers (CPDs) and 6–4 photoproducts (6-4PPs)] and apoptosis were measured using the enzyme-linked immunosorbent assay and the two-color TUNEL (terminal deoxynucleotide transferase dUTP nick end labeling) assay, respectively. The results showed that arsenite reduced the repair rate of 6-4PPs by about a factor of 2 at 5.0 μM and had no effect at 2.5 μM. UVR-induced apoptosis at 24 hr was decreased by 22.64% at 2.5 μM arsenite and by 61.90% at 5.0 μM arsenite. Arsenite decreased the UVR-induced caspase-3/7 activity in parallel with the inhibition of apoptosis. Colony survival assays of the 291.03C cells demonstrate a median lethal concentration (LC(50)) of arsenite of 0.9 μM and a median lethal dose (LD(50)) of UVR of 0.05 kJ/m(2). If the present results are applicable in vivo, inhibition of UVR-induced apoptosis may contribute to arsenite’s enhancement of UVR-induced skin carcinogenesis

Beam normal spin asymmetry in elastic lepton-nucleon scattering

We discuss the two-photon exchange contribution to observables which involve lepton helicity flip in elastic lepton-nucleon scattering. This contribution is accessed through the spin asymmetry for a lepton beam polarized normal to the scattering plane. We estimate this beam normal spin asymmetry at large momentum transfer using a parton model and we express the corresponding amplitude in terms of generalized parton distributions.Comment: 17 pages, 5 figure

Determination of gluon polarization from deep inelastic scattering and collider data

We investigate impact of $\pi^0$-production data at Relativistic Heavy Ion Collider (RHIC) and future E07-011 experiment for the structure function $g_1$ of the deuteron at the Thomas Jefferson National Accelerator Facility (JLab) on studies of nucleonic spin structure, especially on the polarized gluon distribution function. By global analyses of polarized lepton-nucleon scattering and the $\pi^0$-production data, polarized parton distribution functions are determined and their uncertainties are estimated by the Hessian method. Two types of the gluon distribution function are investigated. One is a positive distribution and the other is a node-type distribution which changes sign at $x \sim 0.1$. Although the RHIC $\pi^0$ data seem to favor the node type for $\Delta g(x)$, it is difficult to determine a precise functional form from the current data. However, it is interesting to find that the gluon distribution $\Delta g(x)$ is positive at large $x$ ($>0.2$) due to constraints from the scaling violation in $g_1$ and RHIC $\pi^0$ data. The JLab-E07-011 measurements for $g_1^d$ should be also able to reduce the gluon uncertainty, and the reduction is comparable to the one by RUN-5 $\pi^0$-production data at RHIC. The reduction is caused by the error correlation between polarized antiquark and gluon distributions and by a next-to-leading-order (NLO) gluonic effect in the structure function $g_1^d$. We find that the JLab-E07-011 data are accurate enough to probe the NLO gluonic term in $g_1$. Both RHIC and JLab data contribute to better determination of the polarized gluon distribution in addition to improvement on polarized quark and antiquark distributions.Comment: 11 pages, 9 eps figures, To be published in Nuclear Physics B. AAC08 FORTRAN package is available at the web site http://spin.riken.bnl.gov/aac

Virtual Compton Scattering at Low Energy and the Generalized Polarizabilities of the Nucleon o

Virtual Compton Scattering on the nucleon: $\gamma^* N \to \gamma N$ is a new and rapidly developing field at low and high energies. This lecture is about the low energy part, i.e. for energies in the $(\gamma p)$ center-of-mass mainly up to the $\Delta(1232)$ resonance region. I review the concept of Generalized Polarizabilities of the Nucleon, and the experiments dedicated to their measurement.Comment: 16 pages, 8 figures. lecture given at the Erice School "Lepton Scattering and the Structure of Hadrons and Nuclei," Erice, 16 - 24 Sept. 2004; to appear in "Progress in Particle and Nuclear Physics

Repair of UV-induced thymine dimers is compromised in cells expressing the E6 protein from human papillomaviruses types 5 and 18

Ultraviolet (UV) irradiation is a major mutagenic environmental agent, causing the appearance of DNA adducts that, if unrepaired, may give rise to mutations. Ultraviolet radiation has been indicated as a major risk factor in the development of nonmelanoma skin cancers; however, recent reports have suggested that infections with human papillomaviruses, a widespread family of epitheliotropic DNA viruses, may also contribute to the tumorigeneic process. Here, we investigated whether expression of the E6 protein from different HPV types interfere with the repair of thymine dimers caused by UV-B radiation. Results show that unrepaired DNA damage can be observed in UV-B-irradiated cells expressing the E6 protein of HPV types found in cervical and epithelial cancers. Moreover, such cells have the ability to overcome the G(1) cell cycle checkpoint induced as a result of unrepaired DNA. (C) 2004 Cancer Research UK

Effect of gluon-exchange pair-currents on the ratio G(E(P))/G(M(P))

The effect of one-gluon-exchange (OGE) pair-currents on the ratio $\mu_p G_E^p/G_M^p$ for the proton is investigated within a nonrelativistic constituent quark model (CQM) starting from $SU(6) \times O(3)$ nucleon wave functions, but with relativistic corrections. We found that the OGE pair-currents are important to reproduce well the ratio $\mu_p G_E^p/G_M^p$. With the assumption that the OGE pair-currents are the driving mechanism for the violation of the scaling law we give a prediction for the ratio $\mu_n G_E^n/G_M^n$ of the neutron.Comment: 5 pages, 4 figure

Relativistically invariant analysis of polarization effects in exclusive deuteron electrodisintegration process

A general formalism for the calculation of the differential cross section and polarization observables, for the process of deuteron electrodisintegration, is developed in the framework of relativistic impulse approximation. A detailed analysis of the general structure of the differential cross section and polarization observables for the $e^-+d\to e^-+n+p$ reaction is derived, using the formalism of the structure functions. The obtained expressions have a general nature and they hold in the one--photon--exchange mechanism, assuming P--invariance of the hadron electromagnetic interaction. The model of relativistic impulse approximation is introduced and the final state interaction is taken into account by means of the unitarization of the helicity amplitudes. A detailed description of the unitarization procedure is also presented. Using different parametrizations of the deuteron wave functions, the following polarization observables are calculated in the kinematical region of quasi--elastic deuteron electrodisintegration: the asymmetry for the scattering of longitudinally polarized electrons on a polarized deuteron target, the proton and neutron polarizations (for longitudinally polarized electron beam or vector--polarized deuteron target). The sensitivity to the neutron electric form factor is also thorougly investigated. The predictions of the model are compared with the results of recent polarization measurements and a good agreement with the existing experimental data has been obtained.Comment: 90 pages, 17 figure

Dispersion relations in real and virtual Compton scattering

A unified presentation is given on the use of dispersion relations in the real and virtual Compton scattering processes off the nucleon. The way in which dispersion relations for Compton scattering amplitudes establish connections between low energy nucleon structure quantities, such as polarizabilities or anomalous magnetic moments, and the nucleon excitation spectrum is reviewed. We discuss various sum rules for forward real and virtual Compton scattering, such as the Gerasimov-Drell-Hearn sum rule and its generalizations, the Burkhardt-Cottingham sum rule, as well as sum rules for forward nucleon polarizabilities, and review their experimental status. Subsequently, we address the general case of real Compton scattering (RCS). Various types of dispersion relations for RCS are presented as tools for extracting nucleon polarizabilities from the RCS data. The information on nucleon polarizabilities gained in this way is reviewed and the nucleon structure information encoded in these quantities is discussed. The dispersion relation formalism is then extended to virtual Compton scattering (VCS). The information on generalized nucleon polarizabilities extracted from recent VCS experiments is described, along with its interpretation in nucleon structure models. As a summary, the physics content of the existing data is discussed and some perspectives for future theoretical and experimental activities in this field are presented.Comment: 120 pages, 42 figures, to appear in Phys. Re