Testicular endocrine activity is upregulated by D-Aspartic acid in the green frog Rana esculenta

This study investigated the involvement of D-aspartic acid (D-Asp) in testicular steroidogenesis of the green frog Rana esculenta and its effect on stimulation of thumb pad morphology and glandular activity, a typical testosterone-dependent secondary sexual characteristic in this amphibian species. In the testis, D-Asp concentrations vary significantly during the reproductive cycle: they are low in pre- and post-reproductive periods, but reach peak levels in the reproductive period (140-236 nmol/g wet tissue). Moreover, the concentrations of D-Asp in the testis through the sexual cycle positively match the testosterone levels in the gonad and the plasma. The racemase activity evaluated during the cycle expresses its peak when D-Asp and testosterone levels are highest, that is, during the reproductive period, confirming the synthesis of D-Asp from L-Asp by an aspartate racemase. Short-term in vivo experiments consisting of a single injection of D-Asp (2.0 micro mol/g body weight) demonstrated that this amino acid accumulates significantly in the testis, and after 3 h its uptake is coupled with a testosterone increase in both testis and plasma. Moreover, within 18 h of amino acid administration, the D-Asp concentration in the testis decreased along with the testosterone titer to prestimulation levels. Other amino acids (L-Asp, D-Glu and L-Glu) used instead of D-Asp were ineffective, confirming that the significant increase in testicular testosterone was a specific feature of this amino acid. In long-term experiments, D-Asp had been administered chronically to frogs caught during the three phases of the reproductive cycle, inducing testosterone increase and 17beta-estradiol decrease in the gonad during the pre- and post-reproductive period, and vice versa during the reproductive period

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Evidence for Orbital Motion of Material Close to the Central Black Hole of Mrk 766

Time-resolved X-ray spectroscopy has been obtained for the narrow line Seyfert galaxy Mrk766 from XMM-Newton observations. We present analysis in the energy-time plane of EPIC pn data in the 4-8 keV band with energy resolution R~50. A component of Fe Ka emission detected in the maps shows a variation of photon energy with time that appears both to be statistically significant and to be consistent with sinusoidal variation. We investigate the interpretation that there exists a component of line emission from matter in a Keplerian orbit around a supermassive black hole. The orbit has a period ~165 ks and a line-of-sight velocity ~13,500 km/s. This yields a lower limit for the central mass of M > 4.9x10^5 solar masses within a radius of 3.6 x 10^13 cm (2.4 A.U.). The orbit parameters are consistent with higher black hole masses, but the lack of any substantial gravitational redshift of the orbit implies an upper limit to the black hole mass of 4.5x10^7 solar masses.Comment: 20 pages, 6 figures (some colour). Accepted for publication in A&A. Only minor changes since V1 (including reordering of Figs 1a & b

A Multi-Wavelength Study of the Nature of Type 1.8/1.9 Seyfert Galaxies

We focus on determining the underlying physical cause of a Seyfert galaxy's appearance as type a 1.8 or 1.9. Are these "intermediate" Seyfert types typical Seyfert 1 nuclei with reddened broad-line regions? Or are they objects with intrinsically weak continua and broad emission lines? We compare measurements of the optical reddening of the narrow and broad-line regions with each other and with the X-ray column derived from XMM-Newton 0.5-10 keV spectra to determine the presence and location of dust in the line of sight. We also searched the literature to see if the objects showed evidence for broad-line variability, and determined if the changes were consistent with a change in reddening or a change in the intrinsic ionizing continuum flux. We find that 10 of 19 objects previously classified as Seyfert 1.8/1.9s received this designation due to their low continuum flux. In four objects the classification was due to BLR reddening, either by the torus or dust structures in the vicinity of the NLR; in the remaining five objects there is not sufficient evidence to favor one scenario over the other. These findings imply that, in general, samples of 1.8/1.9s are not suitable for use in studies of the gas and dust in the central torus.Comment: 85 pages, accepted by Ap

Optical Monitoring of 3C 390.3 from 1995 to 2004 and Possible Periodicities in the Historical Light Curve

We report V, R, and I band CCD photometry of the radio galaxy 3C 390.3 obtained with the 1.56-m telescope of the Shanghai Astronomical Observatory from March 1995 to August 2004. Combining these data with data from the literature, we have constructed a historical light curve from 1894 to 2004 and searched for periodicities using the CLEANest program. We find possible periods of 8.30+-1.17, 5.37+-0.49, 3.51+-0.21, and 2.13+-0.08 years.Comment: Accepted by AJ, 34 pages, 11 figure

The optical emission line spectrum of Mark 110

We analyse in detail the rich emission line spectrum of Mark 110 to determine the physical conditions in the nucleus of this object, a peculiar NLS1 without any detectable Fe II emission associated with the broad line region and with a $\lambda5007/H\beta$ line ratio unusually large for a NLS1. We use 24 spectra obtained with the Marcario Low Resolution Spectrograph attached at the prime focus of the 9.2 m Hobby-Eberly telescope at the McDonald observatory. We fitted the spectrum by identifying all the emission lines (about 220) detected in the wavelength range 4200-6900 \AA (at rest). The narrow emission lines are probably produced in a region with a density gradient in the range 10$^{3}-10^{6}$ cm$^{-3}$ with a rather high column density (5$\times10^{21}$ cm$^{-2}$). In addition to a narrow line system, three major broad line systems with different line velocity and width are required. We confirm the absence of broad Fe II emission lines. We speculate that Mark 110 is in fact a BLS1 with relatively "narrow" broad lines but with a BH mass large enough compared to its luminosity to have a lower than Eddington luminosity.Comment: 13 pages, 5 figures, accepted by A&

Discovery of multiple Lorentzian components in the X-ray timing properties of the Narrow Line Seyfert 1 Ark 564

We present a power spectral analysis of a 100 ksec XMM-Newton observation of the narrow line Seyfert 1 galaxy Ark~564. When combined with earlier RXTE and ASCA observations, these data produce a power spectrum covering seven decades of frequency which is well described by a power law with two very clear breaks. This shape is unlike the power spectra of almost all other AGN observed so far, which have only one detected break, and resemble Galactic binary systems in a soft state. The power spectrum can also be well described by the sum of two Lorentzian-shaped components, the one at higher frequencies having a hard spectrum, similar to those seen in Galactic binary systems. Previously we have demonstrated that the lag of the hard band variations relative to the soft band in Ark 564 is dependent on variability time-scale, as seen in Galactic binary sources. Here we show that the time-scale dependence of the lags can be described well using the same two-Lorentzian model which describes the power spectrum, assuming that each Lorentzian component has a distinct time lag. Thus all X-ray timing evidence points strongly to two discrete, localised, regions as the origin of most of the variability. Similar behaviour is seen in Galactic X-ray binary systems in most states other than the soft state, i.e. in the low-hard and intermediate/very high states. Given the very high accretion rate of Ark 564 the closest analogy is with the very high (intermediate) state rather than the low-hard state. We therefore strengthen the comparison between AGN and Galactic binary sources beyond previous studies by extending it to the previously poorly studied very high accretion rate regime.Comment: 11 pages, 11 figures, accepted for publication in MNRA

Effect of COVID-19 pandemic on utilisation of community-based mental health care in North-East of Italy: A psychiatric case register study

Aims: WHO declared that mental health care should be considered one essential health service to be maintained during the coronavirus disease 2019 (COVID-19) pandemic. This study aims to describe the effect of lockdown and restrictions due to the COVID-19 pandemic in Italy on mental health services' utilisation, by considering psychiatric diagnoses and type of mental health contacts. Methods: The study was conducted in the Verona catchment area, located in the Veneto region (northeastern Italy). For each patient, mental health contacts were grouped into: (1) outpatient care, (2) social and supportive interventions, (3) rehabilitation interventions, (4) multi-professional assessments, (5) day care. A 'difference in differences' approach was used: difference in the number of contacts between 2019 and 2020 on the weeks of lockdown and intermediate restrictions was compared with the same difference in weeks of no or reduced restrictions, and such difference was interpreted as the effect of restrictions. Both a global regression on all contacts and separate regressions for each type of service were performed and Incidence Rate Ratios (IRRs) were calculated. Results: In 2020, a significant reduction in the number of patients who had mental health contacts was found, both overall and for most of the patients' characteristics considered (except for people aged 18-24 years for foreign-born population and for those with a diagnosis of schizophrenia. Moreover, in 2020 mental health contacts had a reduction of 57 096 (-33.9%) with respect to 2019; such difference remained significant across the various type of contacts considered, with rehabilitation interventions and day care showing the greatest reduction. Negative Binomial regressions displayed a statistically significant effect of lockdown, but not of intermediate restrictions, in terms of reduction in the number of contacts. The lockdown period was responsible of a 32.7% reduction (IRR 0.673; p-value <0.001) in the overall number of contacts. All type of mental health contacts showed a reduction ascribable to the lockdown, except social and supportive interventions. Conclusions: Despite the access to community mental health care during the pandemic was overall reduced, the mental health system in the Verona catchment area was able to maintain support for more vulnerable and severely ill patients, by providing continuity of care and day-by-day support through social and supportive interventions

Hammering K-wires is Superior to Drilling with Irrigation

Cooling during drilling Kirschner wires is not always effective in preventing thermal related damage. In this study, we used a human in vitro model and compared temperature elevation, insertion time, and extraction force between three Kirschner wire insertion methods—drilling with and without irrigation and pneumatic hammering. Forty five Kirschner wires were inserted into 15 fresh human cadaver metacarpals. All three insertion methods were applied in each metacarpal. Drilling without irrigation resulted in a temperature elevation of 67.25 ± 5.4 ºC with significantly lower values for drilling with irrigation (4.15 ± 0.6 ºC) and pneumatic hammering (31.52 ± 3.4 ºC). The insertion time for pneumatic hammering (47.63 ± 8.8 s) was significantly lower compared to drilling without irrigation (263.16 ± 36.5 s) and drilling with irrigation (196.10 ± 28.5 s). Extraction forces after drilling without irrigation, drilling with irrigation, and pneumatic hammering were 39.85 ± 4.1 N, 57.81 ± 6.5 N, and 62.23 ± 6.7 N, respectively. Pneumatic hammering is superior to drilling without irrigation, especially when irrigation is not possible