MECHANICAL AND CHEMICAL CHARACTERIZATION OF BIOLOGICAL COMPOSITE STRUCTURES

A better understanding of the bioresponse of naturally occurring systems will help to optimize the engineering of synthetic biomaterials. The aim of this thesis was to characterize the mechanical and chemical behavior of two distinct biological composite systems, human teeth and wool fibers. These mechanical and chemical properties were also studied as a function of natural structure and environmental conditions. Human teeth are composite systems consisting primarily of hydroxylapatite and protein. This project investigated how the use of clinical dental treatments and procedures, such as whitening and etching, affects mechanical properties. Analysis of nanoindentation with the Oliver-Pharr model provided elastic modulus and hardness across the DEJ. Mechanical properties of autoclaved and non-autoclaved teeth were measured to ensure both comparability to published values and relevance to clinical applications. Large increases were observed in the elastic modulus of enamel with autoclaving (52.0GPa versus 113.4GPa), while smaller increases were observed in the dentin (17.9GPa versus 27.9GPa). There was a similar trend in the increase in hardness of enamel (2.0GPa versus 4.3GPa) and dentin (0.5GPa versus 0.7GPa) when subjected to autoclaving. This work shows that the range of values previously reported in literature may be due largely to the sterilization procedures. Treatment of the exterior of nonautoclaved teeth with Crest Whitestrips, Opalescence or UltraEtch caused changes in the mechanical properties of both the enamel and dentin. Those treated with Crest Whitestrips showed a reduction in the elastic modulus of enamel (55.3GPa to 32.7GPa) and increase in the elastic modulus of dentin (17.2GPa to 24.3GPa). Opalescence treatments did not show a significant affect on the enamel properties, but did result in a decrease in modulus of dentin (18.5GPa to 15.1GPa). Additionally, UltraEtch treatment decreased the modulus and hardness of enamel (48.7GPa to 38.0GPa and 1.9GPa to 1.5GPa, respectively) and dentin (21.4GPa to 15.0GPa and 1.9GPa to 1.5GPa, respectively). These changes were linked to the change in protein content, verified by FTIR and fluorescence microscopy. The second study characterized the amino acid distribution on the surface of Merino wool fibers. Although previous research identified which amino acids compose wool fibers, this was the first study to determine the amino acids distribution along the surface. Specifically, which amino acids have high concentrations near topographical surface features, such as the scale ridges. The distribution and types of amino acids along the surface of wool fibers was analyzed using force spectroscopy techniques. Initial measurements in phosphate buffer solution (PBS) showed carboxyl acid groups, aspartic acids and glutamic acids, are randomly distributed over the surface of wool fibers. Clusters of sulfur groups, cysteines, are uniformly distributed. In addition, the amine groups, arginine and lysine, are concentrated near the edge of scales. SEM images of wool fibers coated with functionalized nanoparticles were used to verify these results. The SEM images showed the binding sites of various charged chemical groups. To distinguish between positively charged surface groups, force spectroscopy was done under elevated pH, indicating a high contribution of lysine just below the edge of the scale

Light Availability and Phytoplankton Growth Beneath Arctic Sea Ice: Integrating Observations and Modeling

Observations of the seasonal light field in the upper Arctic Ocean are critical to understanding the impacts of changing Arctic ice conditions on phytoplankton growth in the water column. Here we discuss data from a new sensor system, deployed in seasonal ice cover north‐east of Utqiaġvik, Alaska in March 2014. The system was designed to provide observations of light and phytoplankton biomass in the water column during the formation of surface melt ponds and the transition from ice to open water. Hourly observations of downwelling irradiance beneath the ice (at 2.9, 6.9, and 17.9 m depths) and phytoplankton biomass (at 2.9 m depth) were transmitted via Iridium satellite from 9 March to 10 November 2014. Evidence of an under‐ice phytoplankton bloom (Chl a ∼8 mg m−3) was seen in June and July. Increases in light intensity observed by the buoy likely resulted from the loss of snow cover and development of surface melt ponds. A bio‐optical model of phytoplankton production supported this probable trigger for the rapid onset of under‐ice phytoplankton growth. Once under‐ice light was no longer a limiting factor for photosynthesis, open water exposure almost marginally increased daily phytoplankton production compared to populations that remained under the adjacent ice. As strong effects of climate change continue to be documented in the Arctic, the insight derived from autonomous buoys will play an increasing role in understanding the dynamics of primary productivity where ice and cloud cover limit the utility of ocean color satellite observations

The effect of review writing on learning engagement in channel partner relationship management

To develop the knowledge and skill sets of channel partner firms, firms increasingly introduce learning programs as part of their relationship management strategies. Yet, the engagement of channel partners in these programs tends to be low though. The current research, conducted in collaboration with a Fortune 100 information technology company, examines ways to strengthen learning engagement. In accordance with self-regulated learning theory, the authors propose and demonstrate that when channel partners write reviews of a learning module that they attended, beyond providing ratings,they are better able to reflect on the relevance of their learning experience and they are further engaged in learning activities. The audience and focus of these written reviews determine the engagement of the channel partner sales personnel. Therefore, review writing is a valuable, informal mechanism to motivate them. These effects are moderated by characteristics of both the channel partner (learning orientation of the salesperson, identification with the manufacturer) and the relationship with the manufacturer (length and exclusivity)

Plasmodium vivax transmission in Africa

Malaria in sub-Saharan Africa has historically been almost exclusively attributed to Plasmodium falciparum (Pf). Current diagnostic and surveillance systems in much of sub-Saharan Africa are not designed to identify or report non-Pf human malaria infections accurately, resulting in a dearth of routine epidemiological data about their significance. The high prevalence of Duffy negativity provided a rationale for excluding the possibility of Plasmodium vivax (Pv) transmission. However, review of varied evidence sources including traveller infections, community prevalence surveys, local clinical case reports, entomological and serological studies contradicts this viewpoint. Here, these data reports are weighted in a unified framework to reflect the strength of evidence of indigenous Pv transmission in terms of diagnostic specificity, size of individual reports and corroboration between evidence sources. Direct evidence was reported from 21 of the 47 malaria-endemic countries studied, while 42 countries were attributed with infections of visiting travellers. Overall, moderate to conclusive evidence of transmission was available from 18 countries, distributed across all parts of the continent. Approximately 86.6 million Duffy positive hosts were at risk of infection in Africa in 2015. Analysis of the mechanisms sustaining Pv transmission across this continent of low frequency of susceptible hosts found that reports of Pv prevalence were consistent with transmission being potentially limited to Duffy positive populations. Finally, reports of apparent Duffy-independent transmission are discussed. While Pv is evidently not a major malaria parasite across most of sub-Saharan Africa, the evidence presented here highlights its widespread low-level endemicity. An increased awareness of Pv as a potential malaria parasite, coupled with policy shifts towards species-specific diagnostics and reporting, will allow a robust assessment of the public health significance of Pv, as well as the other neglected non-Pf parasites, which are currently invisible to most public health authorities in Africa, but which can cause severe clinical illness and require specific control intervention

Leadership Reconsidered: Engaging Higher Education in Social Change

Colleges and universities can provide effective environments for the development of future leaders. This book addresses the application of transformative leadership to higher education, identifies resources to use in the process, and..

Sectoral Impacts of Invasive Species in the United States and Approaches to Management

Invasive species have a major effect on many sectors of the U.S. economy and on the well-being of its citizens. Their presence impacts animal and human health, military readiness, urban vegetation and infrastructure, water, energy and transportations systems, and indigenous peoples in the United States (Table 9.1). They alter bio-physical systems and cultural practices and require significant public and private expenditure for control. This chapter provides examples of the impacts to human systems and explains mechanisms of invasive species’ establishment and spread within sectors of the U.S. economy. The chapter is not intended to be comprehensive but rather to provide insight into the range and severity of impacts. Examples provide context for ongoing Federal programs and initiatives and support State and private efforts to prevent the introduction and spread of invasive species and eradicate and control established invasive species

Research-Based Web Design & Usability Guidelines [2006 edition]

The new edition of the U.S. Department of Health and Human Services’ (HHS) Research-Based Web Design and Usability Guidelines. These guidelines reflect HHS’ commitment to identifying innovative, research-based approaches that result in highly responsive and easy-to-use Web sites for the public. These guidelines help move us in that direction by providing practical, yet authoritative, guidance on a broad range of Web design and communication issues. Having access to the best available research helps to ensure we make the right decisions the first time around and reduces the possibility of errors and costly mistakes

CCR5 Haplotypes and Mother-to-Child HIV Transmission in Malawi

CCR5 and CCR2 gene polymorphisms (SNPs) have been associated with protection against HIV transmission in adults and with delayed progression to AIDS. The CCR5 Delta32 deletion and SNP -2459G are associated with reduced expression of the CCR5 protein.We investigated the association between infant CCR2/CCR5 diplotype and HIV mother to child transmission (MTCT) in Malawi. Blood samples from infants (n = 552) of HIV positive women who received nevirapine were genotyped using a post-PCR multiplex ligase detection reaction and haplotypes were identified based on 8 CCR2/CCR5 SNPs and the open reading frame 32 base pair deletion. Following verification of Hardy-Weinberg equilibrium, log linear regression was performed to examine the association between mutations and MTCT. Overall, protection against MTCT was weakly associated with two CCR5 SNPs, -2459G (Risk ratio [RR], 0.78; confidence interval [CI], 0.54-1.12), and the linked CCR5 -2135T (RR, 0.78; CI, 0.54-1.13). No child carried the CCR5 Delta32 SNP. Maternal Viral Load (MVL) was found to be an effect measure modifier. Among mothers with low MVL, statistically significant protection against MTCT was observed for -2459G (RR, 0.50; CI, 0.27-0.91), and -2135T (RR, 0.51; CI, 0.28-0.92). Statistically significant protection was not found at high MVL.Results from this study suggest that CCR5 SNPs -2459G and -2135T associated with reduced receptor expression protect against MTCT of HIV at low MVLs, whereas high MVLs may over-ride differences in coreceptor availability

The Long-Baseline Neutrino Experiment: Exploring Fundamental Symmetries of the Universe

The preponderance of matter over antimatter in the early Universe, the dynamics of the supernova bursts that produced the heavy elements necessary for life and whether protons eventually decay --- these mysteries at the forefront of particle physics and astrophysics are key to understanding the early evolution of our Universe, its current state and its eventual fate. The Long-Baseline Neutrino Experiment (LBNE) represents an extensively developed plan for a world-class experiment dedicated to addressing these questions. LBNE is conceived around three central components: (1) a new, high-intensity neutrino source generated from a megawatt-class proton accelerator at Fermi National Accelerator Laboratory, (2) a near neutrino detector just downstream of the source, and (3) a massive liquid argon time-projection chamber deployed as a far detector deep underground at the Sanford Underground Research Facility. This facility, located at the site of the former Homestake Mine in Lead, South Dakota, is approximately 1,300 km from the neutrino source at Fermilab -- a distance (baseline) that delivers optimal sensitivity to neutrino charge-parity symmetry violation and mass ordering effects. This ambitious yet cost-effective design incorporates scalability and flexibility and can accommodate a variety of upgrades and contributions. With its exceptional combination of experimental configuration, technical capabilities, and potential for transformative discoveries, LBNE promises to be a vital facility for the field of particle physics worldwide, providing physicists from around the globe with opportunities to collaborate in a twenty to thirty year program of exciting science. In this document we provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess.Comment: Major update of previous version. This is the reference document for LBNE science program and current status. Chapters 1, 3, and 9 provide a comprehensive overview of LBNE's scientific objectives, its place in the landscape of neutrino physics worldwide, the technologies it will incorporate and the capabilities it will possess. 288 pages, 116 figure

The genetic architecture of the human cerebral cortex

The cerebral cortex underlies our complex cognitive capabilities, yet little is known about the specific genetic loci that influence human cortical structure. To identify genetic variants that affect cortical structure, we conducted a genome-wide association meta-analysis of brain magnetic resonance imaging data from 51,665 individuals. We analyzed the surface area and average thickness of the whole cortex and 34 regions with known functional specializations. We identified 199 significant loci and found significant enrichment for loci influencing total surface area within regulatory elements that are active during prenatal cortical development, supporting the radial unit hypothesis. Loci that affect regional surface area cluster near genes in Wnt signaling pathways, which influence progenitor expansion and areal identity. Variation in cortical structure is genetically correlated with cognitive function, Parkinson's disease, insomnia, depression, neuroticism, and attention deficit hyperactivity disorder