6,011 research outputs found
Eliminating grammatical function assignment from hierarchical models of speech production: Evidence from the conceptual accessibility of referents
ABSTRACTThe assignment of grammatical functions has been a key feature of hierarchical (serial) models of speech production since their inception in the 1970s. This article argues that grammatical function assignment is neither sufficient nor necessary in such models. It reports a study of the effects of the conceptual accessibility of referents on the selection of English dative syntactic frames in production and shows that the effects relate to linear precedence rather than grammatical function assignment. A secondary topic addressed in the same study is whether second language speakers of English have difficulty integrating syntactic knowledge where it interfaces with conceptual accessibility in speech production. Findings suggest that advanced proficiency speakers do not and are qualitatively similar to native speakers. The implications of this for the interface hypothesis about second language acquisition are discussed.</jats:p
Estimation of latent variable models for ordinal data via fully exponential Laplace approximation
Latent variable models for ordinal data represent a useful tool in different
fields of research in which the constructs of interest are not directly
observable. In such models, problems related to the integration of the
likelihood function can arise since analytical solutions do not exist.
Numerical approximations, like the widely used Gauss Hermite (GH) quadrature,
are generally applied to solve these problems. However, GH becomes unfeasible
as the number of latent variables increases. Thus, alternative solutions have
to be found. In this paper, we propose an extended version of the Laplace
method for approximating the integrals, known as fully exponential Laplace
approximation. It is computational feasible also in presence of many latent
variables, and it is more accurate than the classical Laplace method
High-level expression of the HIV entry inhibitor griffithsin from the plastid genome and retention of biological activity in dried tobacco leaves
The global HIV epidemic continues to grow, with 1.8 million new infections occurring per year. In the absence of a cure and an AIDS vaccine, there is a pressing need to prevent new infections in order to curb the disease. Topical microbicides that block viral entry into human cells can potentially prevent HIV infection. The antiviral lectin griffithsin has been identified as a highly potent inhibitor of HIV entry into human cells. Here we have explored the possibility to use transplastomic plants as an inexpensive production platform for griffithsin. We show that griffithsin accumulates in stably transformed tobacco chloroplasts to up to 5% of the total soluble protein of the plant. Griffithsin can be easily purified from leaf material and shows similarly high virus neutralization activity as griffithsin protein recombinantly expressed in bacteria. We also show that dried tobacco provides a storable source material for griffithsin purification, thus enabling quick scale-up of production on demand
A Multi-wavelength Study of the Sunyaev-Zel'dovich Effect in the Triple-Merger Cluster MACS J0717.5+3745 with MUSTANG and Bolocam
We present 90, 140, and 268GHz sub-arcminute resolution imaging of the
Sunyaev-Zel'dovich effect (SZE) in MACSJ0717.5+3745. Our 90GHz SZE data result
in a sensitive, 34uJy/bm map at 13" resolution using MUSTANG. Our 140 and
268GHz SZE imaging, with resolutions of 58" and 31" and sensitivities of 1.8
and 3.3mJy/beam respectively, was obtained using Bolocam. We compare these maps
to a 2-dimensional pressure map derived from Chandra X-ray observations. Our
MUSTANG data confirm previous indications from Chandra of a pressure
enhancement due to shock-heated, >20keV gas immediately adjacent to extended
radio emission seen in low-frequency radio maps. The MUSTANG data also detect
pressure substructure that is not well-constrained by the X-ray data in the
remnant core of a merging subcluster. We find that the small-scale pressure
enhancements in the MUSTANG data amount to ~2% of the total pressure measured
in the 140GHz Bolocam observations. The X-ray template also fails on larger
scales to accurately describe the Bolocam data, particularly at the location of
a subcluster known to have a high line of sight optical velocity (~3200km/s).
Our Bolocam data are adequately described when we add an additional component -
not described by a thermal SZE spectrum - coincident with this subcluster.
Using flux densities extracted from our model fits, and marginalizing over the
temperature constraints for the region, we fit a thermal+kinetic SZE spectrum
to our data and find the subcluster has a best-fit line of sight proper
velocity of 3600+3440/-2160km/s. This agrees with the optical velocity
estimates for the subcluster. The probability of velocity<0 given our
measurements is 2.1%. Repeating this analysis using flux densities measured
non-parametrically results in a 3.4% probability of a velocity<=0. We note that
this tantalizing result for the kinetic SZE is on resolved, subcluster scales.Comment: 10 Figures, 18 pages. this version corrects issues with the previous
arXiv versio
Selection of Shine-Dalgarno sequences in plastids
Like bacterial genes, most plastid (chloroplast) genes are arranged in operons and transcribed as polycistronic mRNAs. Plastid protein biosynthesis occurs on bacterial-type 70S ribosomes and translation initiation of many (but not all) mRNAs is mediated by Shine-Dalgarno (SD) sequences. To study the mechanisms of SD sequence recognition, we have analyzed translation initiation from mRNAs containing multiple SD sequences. Comparing translational efficiencies of identical transgenic mRNAs in Escherichia coli and plastids, we find surprising differences between the two systems. Most importantly, while internal SD sequences are efficiently recognized in E. coli, plastids exhibit a bias toward utilizing predominantly the 5âČ-most SD sequence. We propose that inefficient recognition of internal SD sequences provides the raison d'ĂȘtre for most plastid polycistronic transcripts undergoing post-transcriptional cleavage into monocistronic mRNAs
HerMES: the rest-frame UV emission and a lensing model for the z= 6.34 luminous dusty starburst galaxy HFLS3
We discuss the rest-frame ultraviolet emission from the starbursting galaxy HFLS3 at a redshift of 6.34. The galaxy was discovered in Herschel/SPIRE data due to its red color in the submillimeter wavelengths from 250 to 500 ÎŒm. Keck/NIRC2 K s -band adaptive optics imaging data showed two potential near-IR counterparts near HFLS3. Previously, the northern galaxy was taken to be in the foreground at z = 2.1, while the southern galaxy was assumed to be HFLS3's near-IR counterpart. The recently acquired Hubble/WFC3 and Advanced Camera for Surveys (ACS) imaging data show conclusively that both optically bright galaxies are in the foreground at z < 6. A new lensing model based on the Hubble imaging data and the millimeter-wave continuum emission yields a magnification factor of 2.2 ± 0.3, with a 95% confidence upper limit on the magnification of 3.5. When corrected for lensing, the instantaneous star formation rate is 1320 M â yrâ1, with the 95% confidence lower limit around 830 M â yrâ1. The dust and stellar masses of HFLS3 from the same spectral energy distribution (SED) models are at the level of 3 Ă 108 M â and ~5 Ă 1010 M â, respectively, with large systematic uncertainties on assumptions related to the SED model. With Hubble/WFC3 images, we also find diffuse near-IR emission about 0.5 arcsec (~3 kpc) to the southwest of HFLS3 that remains undetected in the ACS imaging data. The emission has a photometric redshift consistent with either z ~ 6 or a dusty galaxy template at z ~ 2
Detection of relic gravitational waves in the CMB: Prospects for CMBPol mission
Detection of relic gravitational waves, through their imprint in the cosmic
microwave background radiation, is one of the most important tasks for the
planned CMBPol mission. In the simplest viable theoretical models the
gravitational wave background is characterized by two parameters, the
tensor-to-scalar ratio and the tensor spectral index . In this paper,
we analyze the potential joint constraints on these two parameters, and
, using the potential observations of the CMBPol mission, which is
expected to detect the relic gravitational waves if . The
influence of the contaminations, including cosmic weak lensing, various
foreground emissions, and systematical errors, is discussed.Comment: 26 pages, 19 figures, 4 tables; JCAP in pres
Environment of the submillimeter-bright massive starburst HFLS3 at 6.34
We describe the search for Lyman-break galaxies (LBGs) near the
sub-millimeter bright starburst galaxy HFLS3 at 6.34 and a study on the
environment of this massive galaxy during the end of reionization.We performed
two independent selections of LBGs on images obtained with the \textit{Gran
Telescopio Canarias} (GTC) and the \textit{Hubble Space Telescope} (HST) by
combining non-detections in bands blueward of the Lyman-break and color
selection. A total of 10 objects fulfilling the LBG selection criteria at
5.5 were selected over the 4.54 and 55.5 arcmin covered by our HST
and GTC images, respectively. The photometric redshift, UV luminosity, and the
star-formation rate of these sources were estimated with models of their
spectral energy distribution. These 6 candidates have physical
properties and number densities in agreement with previous results. The UV
luminosity function at 6 and a Voronoi tessellation analysis of this
field shows no strong evidence for an overdensity of relatively bright objects
(m25.9) associated with \textit{HFLS3}. However, the over-density
parameter deduced from this field and the surface density of objects can not
excluded definitively the LBG over-density hypothesis. Moreover we identified
three faint objects at less than three arcseconds from \textit{HFLS3} with
color consistent with those expected for 6 galaxies. Deeper data are
needed to confirm their redshifts and to study their association with
\textit{HFLS3} and the galaxy merger that may be responsible for the massive
starburst.Comment: 14 pages, 12 figures, accepted for publication in Ap
Lentiviral Vector Delivery of Human Interleukin-7 (hIL-7) to Human Immune System (HIS) Mice Expands T Lymphocyte Populations
Genetically modified mice carrying engrafted human tissues provide useful models to study human cell biology in physiologically relevant contexts. However, there remain several obstacles limiting the compatibility of human cells within their mouse hosts. Among these is inadequate cross-reactvitiy between certain mouse cytokines and human cellular receptors, depriving the graft of important survival and growth signals. To circumvent this problem, we utilized a lentivirus-based delivery system to express physiologically relevant levels of human interleukin-7 (hIL-7) in Rag2-/-Îłc-/- mice following a single intravenous injection. hIL-7 promoted homeostatic proliferation of both adoptively transferred and endogenously generated T-cells in Rag2-/-Îłc-/- Human Immune System (HIS) mice. Interestingly, we found that hIL-7 increased T lymphocyte numbers in the spleens of HIV infected HIS mice without affecting viral load. Taken together, our study unveils a versatile approach to deliver human cytokines to HIS mice, to both improve engraftment and determine the impact of cytokines on human diseases
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