On the nature of the iron sulfur cluster in a deuterated algal ferredoxin

A protonated and a completely deuterated two-iron algal ferredoxin from Synechococcus lividus have been studied by optical, electron paramagnetic resonance, electron-nuclear double resonance, proton magnetic resonance and Mossbauer spectroscopies; temperature dependent magnetic susceptibility measurements are reported as well. These studies have confirmed the electron localized model of the active center in the two-iron ferredoxins, as previously deduced from studies of spinach ferredoxin, have yielded much more precise spectroscopic parameters for this center, and have thus greatly increased the confidence in this model.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/21928/1/0000335.pd

An activity-based-parametric hybrid cost model to estimate the unit cost of a novel gas turbine component

The first tool presented in this paper is a generic factory cost model that can estimate various costs at multiple levels of any manufacturing plant. The model is activity based which means that the cost of each manufacturing operation is calculated and then summed up so that the true £-per-hour factory cost rate as well as the exact unit cost (i.e. manufacturing cost) of an unlimited number of different components can be estimated.The second tool is a scalable cost model that predicts the unit cost of future integrally bladed disc (blisk) designs that are found in gas turbine compressors. The tool multiplies the machine cost rates, calculated by the factory cost model, by the operation times derived from blisk scaling rules. As the operation times often depend on the number of blades, the disc diameter and other design variables, many scaling rules are based on the correlation between operation times and certain design parameters. Conversely, the remaining process times are constant because they are independent of the blisk geometry. As future process times can only be estimated and the correlation between operation times and design parameters is never perfect, all operation times have uncertainty distributions. These are cascaded through the model to generate a probability distribution of the unit cost.Through the interactive exchange of detailed cost information at the manufacturing operation level as well as extrapolated operation times, the two cost models facilitate design and manufacturing engineering to concurrently optimise blisk designs and manufacturing processes in terms of cost

Discovery of X-rays from Venus with Chandra

On January 10 and 13, 2001, Venus was observed for the first time with an X-ray astronomy satellite. The observation, performed with the ACIS-I and LETG/ACIS-S instruments on Chandra, yielded data of high spatial, spectral, and temporal resolution. Venus is clearly detected as a half-lit crescent, with considerable brightening on the sunward limb. The morphology agrees well with that expected from fluorescent scattering of solar X-rays in the planetary atmosphere. The radiation is observed at discrete energies, mainly at the O-K_alpha energy of 0.53 keV. Fluorescent radiation is also detected from C-K_alpha at 0.28 keV and, marginally, from N-K_alpha at 0.40 keV. An additional emission line is indicated at 0.29 keV, which might be the signature of the C 1s --> pi* transition in CO_2 and CO. Evidence for temporal variability of the X-ray flux was found at the 2.6 sigma level, with fluctuations by factors of a few times indicated on time scales of minutes. All these findings are fully consistent with fluorescent scattering of solar X-rays. No other source of X-ray emission was detected, in particular none from charge exchange interactions between highly charged heavy solar wind ions and atmospheric neutrals, the dominant process for the X-ray emission of comets. This is in agreement with the sensitivity of the observation.Comment: 12 pages, 9 figure

Antibiotherapy and pathogenesis of uncomplicated UTI: difficult relationships

In a time when conventional antibiotics are becoming increasingly less effective for treatment of infections, the relationship between bacteria and antimicrobial resistance is becoming more and more complicated. This paper provides a current review of studies reported in the literature pertaining to the antibiotherapy of human urinary tract infections (UTI), in a way that helps the reader direct a bibliographic search and develop an integrated perspective of the subject. Highlights are given to (bio)pathogenesis of uncomplicated cystitis. Features associated with the antibiotherapy of UTI such as development of resistance are presented in the text systematically. This review discusses recent advances in the understanding of how the predominant uropathogen Escherichia coli interacts with its host and leads to infection; so one can understand some of the reasons behind antibiotherapy failures

Ebola virus epidemiology, transmission, and evolution during seven months in Sierra Leone

The 2013-2015 Ebola virus disease (EVD) epidemic is caused by the Makona variant of Ebola virus (EBOV). Early in the epidemic, genome sequencing provided insights into virus evolution and transmission and offered important information for outbreak response. Here, we analyze sequences from 232 patients sampled over 7 months in Sierra Leone, along with 86 previously released genomes from earlier in the epidemic. We confirm sustained human-to-human transmission within Sierra Leone and find no evidence for import or export of EBOV across national borders after its initial introduction. Using high-depth replicate sequencing, we observe both host-to-host transmission and recurrent emergence of intrahost genetic variants. We trace the increasing impact of purifying selection in suppressing the accumulation of nonsynonymous mutations over time. Finally, we note changes in the mucin-like domain of EBOV glycoprotein that merit further investigation. These findings clarify the movement of EBOV within the region and describe viral evolution during prolonged human-to-human transmission

Genomic sister-disorders of neurodevelopment: an evolutionary approach

Genomic sister-disorders are defined here as diseases mediated by duplications versus deletions of the same region. Such disorders can provide unique information concerning the genomic underpinnings of human neurodevelopment because effects of diametric variation in gene copy number on cognitive and behavioral phenotypes can be inferred. We describe evidence from the literature on deletions versus duplications for the regions underlying the best-known human neurogenetic sister-disorders, including Williams syndrome, Velocardiofacial syndrome, and Smith–Magenis syndrome, as well as the X-chromosomal conditions Klinefelter and Turner syndromes. These data suggest that diametric copy-number alterations can, like diametric alterations to imprinted genes, generate contrasting phenotypes associated with autistic-spectrum and psychotic-spectrum conditions. Genomically based perturbations to the development of the human social brain are thus apparently mediated to a notable degree by effects of variation in gene copy number. We also conducted the first analyses of positive selection for genes in the regions affected by these disorders. We found evidence consistent with adaptive evolution of protein-coding genes, or selective sweeps, for three of the four sets of sister-syndromes analyzed. These studies of selection facilitate identification of candidate genes for the phenotypes observed and lend a novel evolutionary dimension to the analysis of human cognitive architecture and neurogenetic disorders

Exploring Predictors of Outcome in the Psychosis Prodrome: Implications for Early Identification and Intervention

Functional disability is a key component of many psychiatric illnesses, particularly schizophrenia. Impairments in social and role functioning are linked to cognitive deficits, a core feature of psychosis. Retrospective analyses demonstrate that substantial functional decline precedes the onset of psychosis. Recent investigations reveal that individuals at clinical-high-risk (CHR) for psychosis show impairments in social relationships, work/school functioning and daily living skills. CHR youth also demonstrate a pattern of impairment across a range of cognitive domains, including social cognition, which is qualitatively similar to that of individuals with schizophrenia. While many studies have sought to elucidate predictors of clinical deterioration, specifically the development of schizophrenia, in such CHR samples, few have investigated factors relevant to psychosocial outcome. This review integrates recent findings regarding cognitive and social-cognitive predictors of outcome in CHR individuals, and proposes potential directions for future research that will contribute to targeted interventions and improved outcome for at-risk youth