Transcriptome profiling of ontogeny in the acridid grasshopper Chorthippus biguttulus

Acridid grasshoppers (Orthoptera:Acrididae) are widely used model organisms for developmental, evolutionary, and neurobiological research. Although there has been recent influx of orthopteran transcriptomic resources, many use pooled ontogenetic stages obscuring information about changes in gene expression during development. Here we developed a de novo transcriptome spanning 7 stages in the life cycle of the acridid grasshopper Chorthippus biguttulus. Samples from different stages encompassing embryonic development through adults were used for transcriptomic profiling, revealing patterns of differential gene expression that highlight processes in the different life stages. These patterns were validated with semi-quantitative RT-PCR. Embryonic development showed a strongly differentiated expression pattern compared to all of the other stages and genes upregulated in this stage were involved in signaling, cellular differentiation, and organ development. Our study is one of the first to examine gene expression during post-embryonic development in a hemimetabolous insect and we found that only the fourth and fifth instars had clusters of genes upregulated during these stages. These genes are involved in various processes ranging from synthesis of biogenic amines to chitin binding. These observations indicate that post-embryonic ontogeny is not a continuous process and that some instars are differentiated. Finally, genes upregulated in the imago were generally involved in aging and immunity. Our study highlights the importance of looking at ontogeny as a whole and indicates promising directions for future research in orthopteran development

Design concepts for the Cherenkov Telescope Array CTA: an advanced facility for ground-based high-energy gamma-ray astronomy

Ground-based gamma-ray astronomy has had a major breakthrough with the impressive results obtained using systems of imaging atmospheric Cherenkov telescopes. Ground-based gamma-ray astronomy has a huge potential in astrophysics, particle physics and cosmology. CTA is an international initiative to build the next generation instrument, with a factor of 5-10 improvement in sensitivity in the 100 GeV-10 TeV range and the extension to energies well below 100 GeV and above 100 TeV. CTA will consist of two arrays (one in the north, one in the south) for full sky coverage and will be operated as open observatory. The design of CTA is based on currently available technology. This document reports on the status and presents the major design concepts of CTA

A Measurement of the Proton Structure Function F ⁣2(x,Q2)F_{\!2}(x,Q^2)

A measurement of the proton structure function $F_{\!2}(x,Q^2)$ is reported for momentum transfer squared $Q^2$ between 4.5 $GeV^2$ and 1600 $GeV^2$ and for Bjorken $x$ between $1.8\cdot10^{-4}$ and 0.13 using data collected by the HERA experiment H1 in 1993. It is observed that $F_{\!2}$ increases significantly with decreasing $x$, confirming our previous measurement made with one tenth of the data available in this analysis. The $Q^2$ dependence is approximately logarithmic over the full kinematic range covered. The subsample of deep inelastic events with a large pseudo-rapidity gap in the hadronic energy flow close to the proton remnant is used to measure the "diffractive" contribution to $F_{\!2}$.Comment: 32 pages, ps, appended as compressed, uuencoded fil

Stabilization of the Substrate Reaction of Horseradish Peroxidase with o-Phenylenediamine in the Enzyme Immunoassay

Peer Reviewe

Estimating trends of Atlantic meridional overturning circulation from long-term hydrographic data and model simulations

The ocean meridional overturning circulation (MOC) plays a central role for the climate in the Atlantic realm. Since scenarios for future climate change indicate a significant reduction of the MOC under global warming, an assessment of variations and trends of the real MOC is important. Using observations at ocean weather ship (OWS) stations and along oceanic sections, we examine the hydrographic information that can be used to determine MOC trends via its signature in water mass properties obtained from model simulations with the climate model ECHAM5/MPI-OM. We show that temperature trends at mid-latitudes provide useful indirect measure of large-scale changes of deep circulation: A mid-depth warming is related to MOC weakening and a cooling to MOC strengthening. Based on our model experiments, we argue that a continuation of measurements at key OWS sites may contribute to a timely detection of a possible future MOC slowdown and to separate the signal from interannual-to-multidecadal MOC variability. The simulations suggest that the subsurface hydrographic information related to MOC has a lower variability than the MOC trend measured directly. Based on our model and the available long-term hydrographic data, we estimate non-significant MOC trends for the last 80 years. For the twenty-first century, however, the model simulations predict a significant MOC decline and accompanied mid-depth warming trend