Intra-hour cloud index forecasting with data assimilation

We introduce a computational framework to forecast cloud index (CI)fields for up to one hour on a spatial domain that covers a city. Such intra-hour CI forecasts are important to produce solar power forecasts of utility scale solar power and distributed rooftop solar. Our method combines a 2D advection model with cloud motion vectors (CMVs)derived from a mesoscale numerical weather prediction (NWP)model and sparse optical flow acting on successive, geostationary satellite images. We use ensemble data assimilation to combine these sources of cloud motion information based on the uncertainty of each data source. Our technique produces forecasts that have similar or lower root mean square error than reference techniques that use only optical flow, NWP CMV fields, or persistence. We describe how the method operates on three representative case studies and present results from 39 cloudy days

The role of bias in explaining the relation between social preference and depressive symptoms in middle childhood

Past research indicates that children's social preference is an important predictor of childhood depressive symptoms. Additionally, children's negative self-perceptions have been identified as a significant predictor of childhood depressive symptoms. However, few studies have examined bias in children's self-perceived social preference by comparing these self-perceptions to peer-reported social preference. This comparison yields both the degree to which a child is accurate in their self-perception, as well as the direction of these inaccuracies, which is known as perceptual bias. The current study compared self-report and peer-report of social preference to examine both positively and negatively biased self-perceptions in middle childhood. The goal was to examine the mediating role of bias in the relation between social preference and depressive symptoms in middle childhood. A series of regression analyses confirmed significant relations between social preference and negatively biased self-perceptions as predictors of children's depressive symptoms. As predicted, negatively biased self-perceptions also predicted depressive symptoms. Negatively biased self-perceptions, known as the underestimation bias, was found to mediate the relation between social preference and depressive symptoms in 5th grade for children. Implications for future research examining the role of social preference and bias in the development childhood depressive symptoms are discussed

SAFETY CLIMATE AND AFTER-ACTION REVIEWS IN THE FIRE SERVICE

Research on safety climate has focused on supervisor attitudes and their relationship to the attitudes of their direct reports. This study examined the relationship of supervisor behaviors on direct report attitudes that in turn affect group level safety climate among firefighters. This study aggregated perceptions of supervisor trust, leader- member exchange, behavioral integrity, supervisor after action review behavior, and safety climate to the group level to examine the relationship between supervisor behaviors, direct report attitudes and group safety climate. This study found that supervisor trust, behavioral integrity, and supervisor after action review behaviors have a strong positive relationship to safety climate. Further, perceptions of trust partially mediate the relationship between supervisor after action review behaviors and group level safety climate. Future research ideas, theoretical advancements, and practical applications are discussed

Negative self-perceptions in preadolescence: the role of parental hostility and peer victimization

The current study examined the influence of interpersonal relationships on self-perceptions, defined as one’s interpretation of their own personal abilities. Interpersonal relationships with parents and peers, children’s competence, and sex play an important role in the development of self-perceptions,. However, no studies have examined these key factors simultaneously. The current study utilized a path analysis to address these gaps by examining the influence of children’s competence and negative interpersonal interactions (parental hosility and peer victimization) on self-perceptions of academic competence and social acceptance. Academic competence was assessed using scores from the WIAT-II at age 7. Social acceptance and peer victimizaiton at age 7 were measured using peer-report of social preference from the sociometric peer nomination procedure. Observational ratings from parent-child interaction tasks were used to capture parental hostility at age 7. Social and academic self-perceptions were measured using self-report from the BASC-2. In the full sample (N = 382), there was a significant direct effect of academic competence on academic self-perceptions. There was also a significant direct effect of peer victimization on social self-perceptions. A multiple group path analysis was conducted to assess for sex differences. The model produced poor fit among males and good fit among females. For females, there was a significant direct effect of social acceptance on social self-perceptions in the female-only path diagram. There was also a significant direct effect of peer victimization on academic self-perceptions among females. Implications for future research examining the role of competence, interpersonal relationships, and sex on self-perceptions are discussed

Extracellular Hsp72 concentration relates to a minimum endogenous criteria during acute exercise-heat exposure

Extracellular heat-shock protein 72 (eHsp72) concentration increases during exercise-heat stress when conditions elicit physiological strain. Differences in severity of environmental and exercise stimuli have elicited varied response to stress. The present study aimed to quantify the extent of increased eHsp72 with increased exogenous heat stress, and determine related endogenous markers of strain in an exercise-heat model. Ten males cycled for 90 min at 50% O2peak in three conditions (TEMP, 20°C/63% RH; HOT, 30.2°C/51%RH; VHOT, 40.0°C/37%RH). Plasma was analysed for eHsp72 pre, immediately post and 24-h post each trial utilising a commercially available ELISA. Increased eHsp72 concentration was observed post VHOT trial (+172.4%) (P<0.05), but not TEMP (-1.9%) or HOT (+25.7%) conditions. eHsp72 returned to baseline values within 24hrs in all conditions. Changes were observed in rectal temperature (Trec), rate of Trec increase, area under the curve for Trec of 38.5°C and 39.0°C, duration Trec ≥ 38.5°C and ≥ 39.0°C, and change in muscle temperature, between VHOT, and TEMP and HOT, but not between TEMP and HOT. Each condition also elicited significantly increasing physiological strain, described by sweat rate, heart rate, physiological strain index, rating of perceived exertion and thermal sensation. Stepwise multiple regression reported rate of Trec increase and change in Trec to be predictors of increased eHsp72 concentration. Data suggests eHsp72 concentration increases once systemic temperature and sympathetic activity exceeds a minimum endogenous criteria elicited during VHOT conditions and is likely to be modulated by large, rapid changes in core temperature

Molecular Structures of Quiescently Grown and Brain-Derived Polymorphic Fibrils of the Alzheimer Amyloid Aβ9-40 Peptide: A Comparison to Agitated Fibrils

The presence of amyloid deposits consisting primarily of Amyloid-β (Aβ) fibril in the brain is a hallmark of Alzheimer's disease (AD). The morphologies of these fibrils are exquisitely sensitive to environmental conditions. Using molecular dynamics simulations combined with data from previously published solid-state NMR experiments, we propose the first atomically detailed structures of two asymmetric polymorphs of the Aβ9-40 peptide fibril. The first corresponds to synthetic fibrils grown under quiescent conditions and the second to fibrils derived from AD patients' brain-extracts. Our core structure in both fibril structures consists of a layered structure in which three cross-β subunits are arranged in six tightly stacked β-sheet layers with an antiparallel hydrophobic-hydrophobic and an antiparallel polar-polar interface. The synthetic and brain-derived structures differ primarily in the side-chain orientation of one β-strand. The presence of a large and continually exposed hydrophobic surface (buried in the symmetric agitated Aβ fibrils) may account for the higher toxicity of the asymmetric fibrils. Our model explains the effects of external perturbations on the fibril lateral architecture as well as the fibrillogenesis inhibiting action of amphiphilic molecules

Cooperation of Mtmr8 with PI3K Regulates Actin Filament Modeling and Muscle Development in Zebrafish

It has been shown that mutations in at least four myotubularin family genes (MTM1, MTMR1, 2 and 13) are causative for human neuromuscular disorders. However, the pathway and regulative mechanism remain unknown.Here, we reported a new role for Mtmr8 in neuromuscular development of zebrafish. Firstly, we cloned and characterized zebrafish Mtmr8, and revealed the expression pattern predominantly in the eye field and somites during early somitogenesis. Using morpholino knockdown, then, we observed that loss-of-function of Mtmr8 led to defects in somitogenesis. Subsequently, the possible underlying mechanism and signal pathway were examined. We first checked the Akt phosphorylation, and observed an increase of Akt phosphorylation in the morphant embryos. Furthermore, we studied the PH/G domain function within Mtmr8. Although the PH/G domain deletion by itself did not result in embryonic defect, addition of PI3K inhibitor LY294002 did give a defective phenotype in the PH/G deletion morphants, indicating that the PH/G domain was essential for Mtmr8's function. Moreover, we investigated the cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development, and found that both Mtmr8-MO1 and Mtmr8-MO2+LY294002 led to the disorganization of the actin cytoskeleton. In addition, we revealed a possible participation of Mtmr8 in the Hedgehog pathway, and cell transplantation experiments showed that Mtmr8 worked in a non-cell autonomous manner in actin modeling.The above data indicate that a conserved functional cooperation of Mtmr8 with PI3K regulates actin filament modeling and muscle development in zebrafish, and reveal a possible participation of Mtmr8 in the Hedgehog pathway. Therefore, this work provides a new clue to study the physiological function of MTM family members

Mitsui-7, heat-treated, and nitrogen-doped multi-walled carbon nanotubes elicit genotoxicity in human lung epithelial cells

Background: The unique physicochemical properties of multi-walled carbon nanotubes (MWCNT) have led to many industrial applications. Due to their low density and small size, MWCNT are easily aerosolized in the workplace making respiratory exposures likely in workers. The International Agency for Research on Cancer designated the pristine Mitsui-7 MWCNT (MWCNT-7) as a Group 2B carcinogen, but there was insufficient data to classify all other MWCNT. Previously, MWCNT exposed to high temperature (MWCNT-HT) or synthesized with nitrogen (MWCNT-ND) have been found to elicit attenuated toxicity; however, their genotoxic and carcinogenic potential are not known. Our aim was to measure the genotoxicity of MWCNT-7 compared to these two physicochemically-altered MWCNTs in human lung epithelial cells (BEAS-2B & SAEC). Results: Dose-dependent partitioning of individual nanotubes in the cell nuclei was observed for each MWCNT material and was greatest for MWCNT-7. Exposure to each MWCNT led to significantly increased mitotic aberrations with multi- and monopolar spindle morphologies and fragmented centrosomes. Quantitative analysis of the spindle pole demonstrated significantly increased centrosome fragmentation from 0.024–2.4 μg/mL of each MWCNT. Significant aneuploidy was measured in a dose-response from each MWCNT-7, HT, and ND; the highest dose of 24 μg/mL produced 67, 61, and 55%, respectively. Chromosome analysis demonstrated significantly increased centromere fragmentation and translocations from each MWCNT at each dose. Following 24 h of exposure to MWCNT-7, ND and/or HT in BEAS-2B a significant arrest in the G1/S phase in the cell cycle occurred, whereas the MWCNT-ND also induced a G2 arrest. Primary SAEC exposed for 24 h to each MWCNT elicited a significantly greater arrest in the G1 and G2 phases. However, SAEC arrested in the G1/S phase after 72 h of exposure. Lastly, a significant increase in clonal growth was observed one month after exposure to 0.024 μg/mL MWCNT-HT & ND. Conclusions: Although MWCNT-HT & ND cause a lower incidence of genotoxicity, all three MWCNTs cause the same type of mitotic and chromosomal disruptions. Chromosomal fragmentation and translocations have not been observed with other nanomaterials. Because in vitro genotoxicity is correlated with in vivo genotoxic response, these studies in primary human lung cells may predict the genotoxic potency in exposed human populations

Two-pion Bose-Einstein correlations in central Pb-Pb collisions at sNN\sqrt{s_{\rm NN}} = 2.76 TeV

The first measurement of two-pion Bose-Einstein correlations in central Pb-Pb collisions at $\sqrt{s_{\rm NN}} = 2.76$ TeV at the Large Hadron Collider is presented. We observe a growing trend with energy now not only for the longitudinal and the outward but also for the sideward pion source radius. The pion homogeneity volume and the decoupling time are significantly larger than those measured at RHIC.Comment: 17 pages, 5 captioned figures, 1 table, authors from page 12, published version, figures at http://aliceinfo.cern.ch/ArtSubmission/node/388