67 research outputs found
Estudio bibliomĂ©trico de Tesis Doctorales sobre psicologĂa del deporte
En el presente trabajo se realiza un anĂĄlisis bibliomĂ©trico de las tesis doctorales sobre psicologĂa del deporte en España. Se utilizĂł la base de datos TESEO en el periodo temporal 1976-2015. La muestra estuvo com- puesta por 93 tesis doctorales sobre las que se analizaron las siguientes variables: universidades mĂĄs productoras, gĂ©nero del autor de la tesis, nĂșmero de directores varones y mujeres, gĂ©nero del presidente del tribunal, nĂșmero de varones y mujeres en el tribunal, tipo de directores, tipo de tribunal, tipo de muestra de la tesis, presencia de la tesis de manera completa o resumida y tipo de contenidos. Para ello se realizĂł una bĂșsqueda avanzada en la base de datos TESEO y un posterior registro de todas las tesis en Microsoft Excel, para el tratamiento y codicaciĂłn de cada una de las variables. Los resultados indican que la Universidad de Valencia es la instituciĂłn que mĂĄs tesis doctorales sobre psicologĂa del deporte ha producido (16), que son mĂĄs los autores (57) que las autoras (36), gĂ©nero masculino por encima del fe- menino en autorĂa de las tesis (57vs36), nĂșmero de directores, presidencia del tribunal (78vs14), componentes del tribunal, tipo de direcciĂłn y tipo de tribunal, a los deportistas profesionales de Ă©lite como la muestra mĂĄs estudiada (24), a la presencia de resĂșmenes por encima de tesis doctorales completas en TESEO (78vs15) y al deporte de rendimiento como contenido mĂĄs predominante entre las tesis (45).
Exploring the perception of barriers to a dual career by student-athletes with/out disabilities
n recent years, there has been an increase in knowledge about the barriers experienced by people with disabilities in the education system or sports. However, no studies have analyzed the barriers for those who try to succeed in both disciplines (dual career). The purpose of this study was to examine the barriers faced by student-athletes with/out disability to a dual career combining studies and sport. Two groups were involved in the study (n = 162): student-athletes with disabilities (n = 79) and student-athletes without disabilities (n = 83). Data collected included: (a) socio-demographic aspects; and (b) barriers towards achieving a good balance between sport and academics during the dual career, through the "Perceptions of dual career student-athletes" (ESTPORT) questionnaire. The results showed that student-athletes with disabilities were more likely to perceive in a greater extent the barriers, the university is far from my home (p = 0.007) and the university is far from my training site (p = 0.006), I find myself unable to balance study and training time (p = 0.030), I have to take care of my family (p<0.001), and my current job does not allow me to study enough (p<0.001). The MANOVA analysis showed that the factors gender, competitive level, and employment status had an influence on the perception of some barriers between groups. In conclusion, student-athletes with disabilities perceived barriers more strongly than those without disabilities, and measures are needed to ensure their inclusion in the education system
The p25 subunit of the dynactin complex is required for dyneinâearly endosome interaction
The p25 subunit of the dynactin complex is required for the interaction between cytoplasmic dynein and early endosomes but is not required for dynein-mediated nuclear distribution
Long-distance endosome trafficking drives fungal effector production during plant infection
To cause plant disease, pathogenic fungi can secrete effector proteins into plant cells to suppress plant immunity and facilitate fungal infection. Most fungal pathogens infect plants using very long strand-like cells, called hyphae, that secrete effectors from their tips into host tissue. How fungi undergo long-distance cell signalling to regulate effector production during infection is not known. Here we show that long-distance retrograde motility of early endosomes (EEs) is necessary to trigger transcription of effector-encoding genes during plant infection by the pathogenic fungus Ustilago maydis. We demonstrate that motor-dependent retrograde EE motility is necessary for regulation of effector production and secretion during host cell invasion. We further show that retrograde signalling involves the mitogen-activated kinase Crk1 that travels on EEs and participates in control of effector production. Fungal pathogens therefore undergo long-range signalling to orchestrate host invasion
Roles of Dynein and Dynactin in Early Endosome Dynamics Revealed Using Automated Tracking and Global Analysis
Microtubule-dependent movement is crucial for the spatial organization of endosomes in most eukaryotes, but as yet there has been no systematic analysis of how a particular microtubule motor contributes to early endosome dynamics. Here we tracked early endosomes labeled with GFP-Rab5 on the nanometer scale, and combined this with global, first passage probability (FPP) analysis to provide an unbiased description of how the minus-end microtubule motor, cytoplasmic dynein, supports endosome motility. Dynein contributes to short-range endosome movement, but in particular drives 85â98% of long, inward translocations. For these, it requires an intact dynactin complex to allow membrane-bound p150Glued to activate dynein, since p50 over-expression, which disrupts the dynactin complex, inhibits inward movement even though dynein and p150Glued remain membrane-bound. Long dynein-dependent movements occur via bursts at up to âŒ8 ”msâ1 that are linked by changes in rate or pauses. These peak speeds during rapid inward endosome movement are still seen when cellular dynein levels are 50-fold reduced by RNAi knock-down of dynein heavy chain, while the number of movements is reduced 5-fold. Altogether, these findings identify how dynein helps define the dynamics of early endosomes
A microscopy-based screen employing multiplex genome sequencing identifies cargo-specific requirements for dynein velocity
The timely delivery of membranous organelles and macromolecules to specific locations within the majority of eukaryotic cells depends on microtubule-based transport. Here, we describe a screening method to identify mutations that have a critical effect on intracellular transport and its regulation using mutagenesis, multicolor-fluorescence microscopy, and multiplex genome sequencing. This screen exploits the filamentous fungus Aspergillus nidulans, which has many of the advantages of yeast molecular genetics, but uses long-range microtubule-based transport in a manner more similar to metazoan cells. Using this method, we identified 7 mutants that represent novel alleles of components of the intracellular transport machinery: specifically, kinesin-1, cytoplasmic dynein, and the dynein regulators Lis1 and dynactin. The two dynein mutations identified in our screen map to dynein's AAA+ catalytic core. Single-molecule studies reveal that both mutations reduce dynein's velocity in vitro. In vivo these mutants severely impair the distribution and velocity of endosomes, a known dynein cargo. In contrast, another dynein cargo, the nucleus, is positioned normally in these mutants. These results reveal that different dynein functions have distinct velocity requirements
The Functions of Myosin II and Myosin V Homologs in Tip Growth and Septation in Aspergillus nidulans
Because of the industrial and medical importance of members of the fungal genus Aspergillus, there is considerable interest in the functions of cytoskeletal components in growth and secretion in these organisms. We have analyzed the genome of Aspergillus nidulans and found that there are two previously unstudied myosin genes, a myosin II homolog, myoB (productâ=âMyoB) and a myosin V homolog, myoE (productâ=âMyoE). Deletions of either cause significant growth defects. MyoB localizes in strings that coalesce into contractile rings at forming septa. It is critical for septation and normal deposition of chitin but not for hyphal extension. MyoE localizes to the Spitzenkörper and to moving puncta in the cytoplasm. Time-lapse imaging of SynA, a v-SNARE, reveals that in myoE deletion strains vesicles no longer localize to the Spitzenkörper. Tip morphology is slightly abnormal and branching occurs more frequently than in controls. Tip extension is slower than in controls, but because hyphal diameter is greater, growth (increase in volume/time) is only slightly reduced. Concentration of vesicles into the Spitzenkörper before incorporation into the plasma membrane is, thus, not required for hyphal growth but facilitates faster tip extension and a more normal hyphal shape
Calcium-containing phosphopeptides pave the secretory pathway for efficient protein traffic and secretion in fungi
About the authors
Research on security-related aspects of climate change is an important element of climate change impact assessments. Hamburg has become a globally recognized center of pertinent analysis of the climate-conflict-nexus. The essays in this collection present a sample of the research conducted from 2009 to 2018 within an interdisciplinary cooperation of experts from UniversitĂ€t Hamburg and other institutions in Hamburg related to the research group âClimate Change and Securityâ (CLISEC). This collection of critical assessments covers a broad understanding of security, ranging from the question of climate change as a cause of violent conflict to conditions of human security in the Anthropocene. The in-depth analyses utilize a wide array of methodological approaches, from agent-based modeling to discourse analysis
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