The helium spread in the Globular cluster 47 Tuc

Spectroscopy has shown the presence of the CN band dicothomy and the Na-O anticorrelations for 50--70% of the investigated samples in the cluster 47 Tuc, otherwise considered a "normal" prototype of high metallicity clusters from the photometric analysis. Very recently, the re-analysis of a large number of archival HST data of the cluster core has been able to put into evidence the presence of structures in the Sub Giant Branch: it has a brighter component with a spread in magnitude by $\sim$0.06 mag and a second one, made of about 10% of stars, a little fainter (by $\sim$0.05 mag). These data also show that the Main Sequence of the cluster has an intrinsic spread in color which, if interpreted as due to a small spread in helium abundance, suggests $\Delta$Y$\sim$0.027. In this work we examine in detail whether the Horizontal Branch morphology and the Sub Giant structure provide further independent indications that a real --although very small-helium spread is present in the cluster. We re--analyze the HST archival data for the Horizontal Branch of 47 Tuc, obtaining a sample of $\sim$500 stars with very small photometric errors, and build population synthesis based on new models to show that its particular morphology can be better explained by taking into account a spread in helium abundance of 2% in mass. The same variation in helium is able to explain the spread in luminosity of the Sub Giant Branch, while a small part of the second generation is characterized by a small C+N+O increase and provides an explanation for the fainter Sub Giant Branch. We conclude that three photometric features concur to form the paradigm that a small but real helium spread is present in a cluster that has no spectacular evidence for multiple populations like those shown by other massive clusters.Comment: Accepted for publication in the MNRAS on 2010 June 8. Received 2010 May 19; in original form 2010 February 9. 7 pages and 3 figures. No table

Análise espacial das mudanças na cobertura e uso das terras em Santarém e Belterra, Pará, Brasil: armadilhas metodológicas associadas.

Através de um estudo de caso, indicamos como a delimitação da área de estudo pode influenciar o resultado de análises multiescalares em processos espaciais de mudanças na cobertura e uso da terra na Amazônia. Partindo dos limites dos municípios de Santarém e Belterra no oeste do Estado do Pará, definimos três níveis de delimitação da área de estudo. O primeiro nível abrange uma região que foi arbitrariamente definida e denominada sub-região de Santarém e Belterra. O segundo nível, uma parte do primeiro, corresponde ao limite do entorno de lotes estabelecidos pelo INCRA na década de 1970, tratando-se portanto de uma área de ocupação consolidada. O terceiro nível corresponde às zonas de influência de quatro eixos viários inseridos dentro da área de ocupação consolidada, subdivididos em subáreas norte e sul, num total de oito subáreas do segundo nível de delimitação. Para cada nível, aplicamos métricas de paisagem sobre mapeamentos temáticos do satélite Landsat obtidos para os anos de 1986 a 2005 e as associamos com dados sociodemográficos obtidos em levantamentos realizados em 2003. Os resultados mostram que as peculiaridades da dinâmica de ocupação em cada nível permitem melhor identificar padrões e processos na composição da estrutura da paisagem. Os dados obtidos para os três níveis de delimitação são complementares, possibilitando uma compreensão mais abrangente do que aquela que se poderia obter pelo estudo de um único nível

A paradigmatic autistic phenotype associated with loss of PCDH11Y and NLGN4Y genes

Background: Most studies relative to Y chromosome abnormalities are focused on the sexual developmental disorders. Recently, a few studies suggest that some genes located on Y chromosome may be related to different neurodevelopment disorders. Case presentation: We report a child with sexual developmental disorder associated with a peculiar phenotype characterized by severe language impairment and autistic behaviour associated with a mosaicism [45,X(11)/46,XY(89)] and a partial deletion of the short and long arm of Y chromosome (del Yp11.31q11.23) that also involves the loss of both PCDH11Y and NLGN4Y genes. To our knowledge no study has ever reported the occurrence of the lack of both PCDH11Y and NLGN4Y located in the Y chromosome in the same patient. Conclusions: We hypothesized a functional complementary role of PCDH11Y and NLGN4Y within formation/maturation of the cerebral cortex. The impairment of early language development may be mainly related to the lack of PCDH11Y that underlies the early language network development and the later appearance of the autistic behaviour may be mainly related to deficit of inhibitory glicinergic neurotransmission NLGN4Y-linked

Commonalities and distinctions between two neurodevelopmental disorder subtypes associated with SCN2A and SCN8A variants and literature review

This study was aimed to analyze the commonalities and distinctions of voltage-gated sodium channels, Nav1.2, Nav1.6, in neurodevelopmental disorders. An observational study was performed including two patients with neurodevelopmental disorders. The demographic, electroclinical, genetic, and neuropsychological characteristics were analyzed and compared with each other and then with the subjects carrying the same genetic variants reported in the literature. The clinical features of one of them argued for autism spectrum disorder and developmental delay, the other for intellectual disability, diagnoses confirmed by the neuropsychological assessment. The first patient was a carrier of SCN2A (p.R379H) variant while the second was carrier of SCN8A (p.E936K) variant, both involving the pore loop of the two channels. The results of this study suggest that the neurodevelopmental disorders without overt epilepsy of both patients can be the consequences of loss of function of Nav1.2/Nav1.6 channels. Notably, the SCN2A variant, with an earlier expression timing in brain development, resulted in a more severe phenotype as autism spectrum disorder and developmental delay, while the SCN8A variant, with a later expression timing, resulted in a less severe phenotype as intellectual disability

Invasive group B streptococcal infections in adults, France (2007–2010)

AbstractGroup B streptococcus (GBS) has emerged as an important cause of invasive infection in adults. Here, we report the clinical and microbiological characteristics of 401 non-redundant GBS strains causing adult invasive infections collected during a 4-year period (2007–2010). Bacteraemia without focus (43.4%) and bone and joint infections (18.7%) were the main clinical manifestations. The distribution of capsular polysaccharide (CPS) type showed that types Ia, III, and V accounted for 71.8% of all strains. Resistance to erythromycin increased from 20.2% in 2007 to 35.3% in 2010, and was mainly associated with CPS type V harbouring the erm(B) resistant determinant

UPO Biobank: The Challenge of Integrating Biobanking into the Academic Environment to Support Translational Research

Biobanks are driving motors of precision and personalized medicine by providing high-quality biological material/data through the standardization and harmonization of their collection, preservation, and distribution. UPO Biobank was established in 2020 as an institutional, disease, and population biobank within the University of Piemonte Orientale (UPO) for the promotion and support of high-quality, multidisciplinary studies. UPO Biobank collaborates with UPO researchers, sustaining academic translational research, and supports the Novara Cohort Study, a longitudinal cohort study involving the population in the Novara area that will collect data and biological specimens that will be available for epidemiological, public health, and biological studies on aging. UPO Biobank has been developed by implementing the quality standards for the field and the ethical and legal issues and normative about privacy protection, data collection, and sharing. As a member of the "Biobanking and Biomolecular Resources Research Infrastructure" (BBMRI) network, UPO Biobank aims to expand its activity worldwide and launch cooperation with new national and international partners and researchers. The objective of this manuscript is to report an institutional and operational experience through the description of the technical and procedural solutions and ethical and scientific implications associated with the establishment of this university research biobank

The first magnetic maps of a pre-main sequence binary star system - HD 155555

We present the first maps of the surface magnetic fields of a pre-main sequence binary system. Spectropolarimetric observations of the young, 18 Myr, HD 155555 (V824 Ara, G5IV + K0IV) system were obtained at the Anglo-Australian Telescope in 2004 and 2007. Both datasets are analysed using a new binary Zeeman Doppler imaging (ZDI) code. This allows us to simultaneously model the contribution of each component to the observed circularly polarised spectra. Stellar brightness maps are also produced for HD 155555 and compared to previous Doppler images. Our radial magnetic maps reveal a complex surface magnetic topology with mixed polarities at all latitudes. We find rings of azimuthal field on both stars, most of which are found to be non-axisymmetric with the stellar rotational axis. We also examine the field strength and the relative fraction of magnetic energy stored in the radial and azimuthal field components at both epochs. A marked weakening of the field strength of the secondary star is observed between the 2004 and 2007 epochs. This is accompanied by an apparent shift in the location of magnetic energy from the azimuthal to radial field. We suggest that this could be indicative of a magnetic activity cycle. We use the radial magnetic maps to extrapolate the coronal field (by assuming a potential field) for each star individually - at present ignoring any possible interaction. The secondary star is found to exhibit an extreme tilt (~75 deg) of its large scale magnetic field to that of its rotation axis for both epochs. The field complexity that is apparent in the surface maps persists out to a significant fraction of the binary separation. Any interaction between the fields of the two stars is therefore likely to be complex also. Modelling this would require a full binary field extrapolation.Comment: 17 pages, 12 figures, accepted for publication in MNRA

Cyclophilin A/EMMPRIN Axis Is Involved in Pro-Fibrotic Processes Associated with Thoracic Aortic Aneurysm of Marfan Syndrome Patients

Background: Marfan syndrome (MFS) is a genetic disease, characterized by thoracic aortic aneurysm (TAA), which treatment is to date purely surgical. Understanding of novel molecular targets is mandatory to unveil effective pharmacological approaches. Cyclophilin A (CyPA) and its receptor EMMPRIN are associated with several cardiovascular diseases, including abdominal aortic aneurysm. Here, we envisioned the contribution of CyPA/EMMPRIN axis in MFS-related TAA. METHODS: We obtained thoracic aortic samples from healthy controls (HC) and MFS patients' aortas and then isolated vascular smooth muscle cells (VSMC) from the aortic wall. RESULTS: our findings revealed that MFS aortic tissue samples isolated from the dilated zone of aorta showed higher expression levels of EMMPRIN vs. MFS non-dilated aorta and HC. Interestingly, angiotensin II significantly stimulated CyPA secretion in MFS-derived VSMC (MFS-VSMC). CyPA treatment on MFS-VSMC led to increased levels of EMMPRIN and other MFS-associated pro-fibrotic mediators, such as TGF-\u3b21 and collagen I. These molecules were downregulated by in vitro treatment with CyPA inhibitor MM284. Our results suggest that CyPA/EMMPRIN axis is involved in MFS-related TAA development, since EMMPRIN is upregulated in the dilated zone of MFS patients' TAA and the inhibition of its ligand, CyPA, downregulated EMMPRIN and MFS-related markers in MFS-VSMC. CONCLUSIONS: these insights suggest both a novel detrimental role for CyPA/EMMPRIN axis and its inhibition as a potential therapeutic strategy for MFS-related TAA treatment

POISSON project - II - A multi-wavelength spectroscopic and photometric survey of young protostars in L 1641

Characterising stellar and circumstellar properties of embedded young stellar objects (YSOs) is mandatory for understanding the early stages of the stellar evolution. This task requires the combination of both spectroscopy and photometry, covering the widest possible wavelength range, to disentangle the various protostellar components and activities. As part of the POISSON project, we present a multi-wavelength spectroscopic and photometric investigation of embedded YSOs in L1641, aimed to derive the stellar parameters and evolutionary stages and to infer their accretion properties. Our database includes low-resolution optical-IR spectra from the NTT and Spitzer (0.6-40 um) and photometric data covering a spectral range from 0.4 to 1100 um, which allow us to construct the YSOs spectral energy distributions (SEDs) and to infer the main stellar parameters. The SED analysis allows us to group our 27 YSOs into nine Class I, eleven Flat, and seven Class II objects. However, on the basis of the derived stellar properties, only six Class I YSOs have an age of ~10^5 yr, while the others are older 5x10^5-10^6 yr), and, among the Flat sources, three out of eleven are more evolved objects (5x10^6-10^7 yr), indicating that geometrical effects can significantly modify the SED shapes. Inferred mass accretion rates (Macc) show a wide range of values (3.6x10^-9 to 1.2x10^-5 M_sun yr^-1), which reflects the age spread observed in our sample. Average values of mass accretion rates, extinction, and spectral indices decrease with the YSO class. The youngest YSOs have the highest Macc, whereas the oldest YSOs do not show any detectable jet activity in either images and spectra. We also observe a clear correlation among the YSO Macc, M*, and age, consistent with mass accretion evolution in viscous disc models.Comment: 61 pages, 16 figures; A&A in pres

Down Regulation of a Matrix Degrading Cysteine Protease Cathepsin L, by Acetaldehyde: Role of C/EBPα

BACKGROUND: The imbalance between extra cellular matrix (ECM) synthesis and degradation is critical aspect of various hepatic pathologies including alcohol induced liver fibrosis. This study was carried out to investigate the effect of acetaldehyde on expression of an extra cellular matrix degrading protease cathepsin L (CTSL) in HepG2 cells. METHODOLOGY AND RESULTS: We measured the enzymatic activity, protein and, mRNA levels of CTSL in acetaldehyde treated and untreated cells. The binding of CAAT enhancer binding protein α (C/EBP α) to CTSL promoter and its key role in the transcription from this promoter and conferring responsiveness to acetaldehyde was established by site directed mutagenesis, electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (ChIP) assays and siRNA technology. Acetaldehyde treatment significantly decreased CTSL activity and protein levels in HepG2 cells. A similar decrease in the mRNA levels and promoter activity was also observed. This decrease by acetaldehyde was attributed to the fall in the liver enriched transcription factor C/EBP α levels and it's binding to the CTSL promoter. Mutagenesis of C/EBP α binding motifs revealed the key role of this factor in CTSL transcription as well as conferring responsiveness to acetaldehyde. The siRNA mediated silencing of the C/EBP α expression mimicked the effect of acetaldehyde on CTSL levels and its promoter activity. It also abolished the responsiveness of this promoter to acetaldehyde. CONCLUSION: Acetaldehyde down regulates the C/EBP α mediated CTSL expression in hepatic cell lines. The decreased expression of CTSL may at least in part contribute to ECM deposition in liver which is a hallmark of alcoholic liver fibrosis