Application of an electronic tongue as a single-run tool for olive oils physicochemical and sensory simultaneous assessment

Olive oil is highly appreciated due to its nutritional and organoleptic characteristics. However, a huge compositional variation is observed between olive oils, requiring the use of diverse analytical techniques for its classification including titration, spectrophotometry and chromatography, as well as sensory analysis. Chemical analysis is usually time-consuming, expensive and require skilled technicians, while the sensorial ones are dependent upon individual subjective evaluations, even if performed by trained panellists. This work evaluated and demonstrated the feasibility of using a potentiometric electronic tongue, comprising non-specific lipid polymeric and cross-sensitive sensor membranes, coupled with chemometric tools based on different sub-sets of sensors (from 11 to 14 sensors), to predict key quality parameters of olive oils based on single-run assays. The multivariate linear models established for 23 centenarian olive trees from different cultivars allowed predicting peroxide value, oxidative stability, total phenols and tocopherols contents, CIELAB scale parameters (L*, a* and b* values), as well as 11 gustatory-retronasal positive attributes (green, sweet, bitter, pungent, tomato and tomato leaves, apple, banana, cabbage, fresh herbs and dry fruits) with satisfactory accuracy (0.90±0.07 R2 0.98±0.02 for the repeated K-fold-CV procedure, which ensured that 25% of the data was used for internal-validation purposes). The electronic tongue device had an accuracy statistically similar to that achieved with standard analytical techniques, pointing out the versatility of the device for the fast and simultaneous chemical and sensory analysis of olive oil.Project POCI-01-0145-FEDER-006984 – Associate Laboratory LSRE-LCM, Project UID/BIO/04469/2013 – CEB, Project UID/QUI/50006/2013 - REQUIMTE-LAQV and strategic project PEst-OE/AGR/UI0690/2014 – CIMO all funded by European Regional Development Fund (ERDF) through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT – Fundação para a Ciência e a Tecnologia I.P.info:eu-repo/semantics/publishedVersio

Semi-Automatic Method for Early Detection of Xylella fastidiosa in Olive Trees Using UAV Multispectral Imagery and Geostatistical-Discriminant Analysis

Xylella fastidiosa subsp. pauca (Xfp) is one of the most dangerous plant pathogens in the world. Identified in 2013 in olive trees in south–eastern Italy, it is spreading to the Mediterranean countries. The bacterium is transmitted by insects that feed on sap, and causes rapid wilting in olive trees. The paper explores the use of Unmanned Aerial Vehicle (UAV) in combination with a multispectral radiometer for early detection of infection. The study was carried out in three olive groves in the Apulia region (Italy) and involved four drone flights from 2017 to 2019. To classify Xfp severity level in olive trees at an early stage, a combined method of geostatistics and discriminant analysis was implemented. The results of cross-validation for the non-parametric classification method were of overall accuracy = 0.69, mean error rate = 0.31, and for the early detection class of accuracy 0.77 and misclassification probability 0.23. The results are promising and encourage the application of UAV technology for the early detection of Xfp infection

Wavelength Selection Method Based on Partial Least Square from Hyperspectral Unmanned Aerial Vehicle Orthomosaic of Irrigated Olive Orchards

Identifying and mapping irrigated areas is essential for a variety of applications such as agricultural planning and water resource management. Irrigated plots are mainly identified using supervised classification of multispectral images from satellite or manned aerial platforms. Recently, hyperspectral sensors on-board Unmanned Aerial Vehicles (UAV) have proven to be useful analytical tools in agriculture due to their high spectral resolution. However, few efforts have been made to identify which wavelengths could be applied to provide relevant information in specific scenarios. In this study, hyperspectral reflectance data from UAV were used to compare the performance of several wavelength selection methods based on Partial Least Square (PLS) regression with the purpose of discriminating two systems of irrigation commonly used in olive orchards. The tested PLS methods include filter methods (Loading Weights, Regression Coefficient and Variable Importance in Projection); Wrapper methods (Genetic Algorithm-PLS, Uninformative Variable Elimination-PLS, Backward Variable Elimination-PLS, Sub-window Permutation Analysis-PLS, Iterative Predictive Weighting-PLS, Regularized Elimination Procedure-PLS, Backward Interval-PLS, Forward Interval-PLS and Competitive Adaptive Reweighted Sampling-PLS); and an Embedded method (Sparse-PLS). In addition, two non-PLS based methods, Lasso and Boruta, were also used. Linear Discriminant Analysis and nonlinear K-Nearest Neighbors techniques were established for identification and assessment. The results indicate that wavelength selection methods, commonly used in other disciplines, provide utility in remote sensing for agronomical purposes, the identification of irrigation techniques being one such example. In addition to the aforementioned, these PLS and non-PLS based methods can play an important role in multivariate analysis, which can be used for subsequent model analysis. Of all the methods evaluated, Genetic Algorithm-PLS and Boruta eliminated nearly 90% of the original spectral wavelengths acquired from a hyperspectral sensor onboard a UAV while increasing the identification accuracy of the classification

Evaluation of Multispectral Data Acquired from UAV Platform in Olive Orchard

Precision agriculture is a management strategy to improve resource efficiency, production, quality, profitability and sustainability of the crops. In recent years, olive tree management is increasingly focused on determining the correct health status of the plants in order to distribute the main resource using different technologies. In the olive grove, the focus is often on the use of multispectral information from UAVs (Unmanned Aerial Vehicle), but it is not known how important spectral and biometric information actually is for the agronomic management of the olive grove. The aim of this study was to investigate the ability of multispectral data acquired from a UAV platform to predict nutritional status, biometric characteristics, vegetative condition and production of olive orchard as tool to DSS. Data were collected on vegetative characteristics closely related to vigour such as trunk cross-sectional area (TCSA), Nitrogen concentration of the leaves, canopy area and canopy volume. The production was collected for each plant to create an accurate yield map. The flight was carried out with a UAV equipped with a multispectral camera, at an altitude of 50 m and with RTK correction. The flight made it possible to determine the biometric condition and the spectral features through the normalized difference vegetation index (NDVI). The NDVI map allowed to determine the canopy area. The Structure for Motion (SfM) algorithm allow to determine the 3D canopy volume. The experiment showed that the NDVI was able to determine with high accuracy the vegetative characteristic as canopy area (r = 0.87 ***), TCSA (r = 0.58 ***) and production (r = 0.63 ***). The vegetative parameters are closely correlated with the production, especially the canopy area (r = 0.75 ***). Data clustering showed that the production of individual plants is closely dependent on leaf nitrogen concentration and vigour status

Electronic Noses and Tongues: Applications for the Food and Pharmaceutical Industries

The electronic nose (e-nose) is designed to crudely mimic the mammalian nose in that most contain sensors that non-selectively interact with odor molecules to produce some sort of signal that is then sent to a computer that uses multivariate statistics to determine patterns in the data. This pattern recognition is used to determine that one sample is similar or different from another based on headspace volatiles. There are different types of e-nose sensors including organic polymers, metal oxides, quartz crystal microbalance and even gas-chromatography (GC) or combined with mass spectroscopy (MS) can be used in a non-selective manner using chemical mass or patterns from a short GC column as an e-nose or “Z” nose. The electronic tongue reacts similarly to non-volatile compounds in a liquid. This review will concentrate on applications of e-nose and e-tongue technology for edible products and pharmaceutical uses

New strategies for row-crop management based on cost-effective remote sensors

Agricultural technology can be an excellent antidote to resource scarcity. Its growth has led to the extensive study of spatial and temporal in-field variability. The challenge of accurate management has been addressed in recent years through the use of accurate high-cost measurement instruments by researchers. However, low rates of technological adoption by farmers motivate the development of alternative technologies based on affordable sensors, in order to improve the sustainability of agricultural biosystems. This doctoral thesis has as main objective the development and evaluation of systems based on affordable sensors, in order to address two of the main aspects affecting the producers: the need of an accurate plant water status characterization to perform a proper irrigation management and the precise weed control. To address the first objective, two data acquisition methodologies based on aerial platforms have been developed, seeking to compare the use of infrared thermometry and thermal imaging to determine the water status of two most relevant row-crops in the region, sugar beet and super high-density olive orchards. From the data obtained, the use of an airborne low-cost infrared sensor to determine the canopy temperature has been validated. Also the reliability of sugar beet canopy temperature as an indicator its of water status has been confirmed. The empirical development of the Crop Water Stress Index (CWSI) has also been carried out from aerial thermal imaging combined with infrared temperature sensors and ground measurements of factors such as water potential or stomatal conductance, validating its usefulness as an indicator of water status in super high-density olive orchards. To contribute to the development of precise weed control systems, a system for detecting tomato plants and measuring the space between them has been developed, aiming to perform intra-row treatments in a localized and precise way. To this end, low cost optical sensors have been used and compared with a commercial LiDAR laser scanner. Correct detection results close to 95% show that the implementation of these sensors can lead to promising advances in the automation of weed control. The micro-level field data collected from the evaluated affordable sensors can help farmers to target operations precisely before plant stress sets in or weeds infestation occurs, paving the path to increase the adoption of Precision Agriculture techniques

Uso de sensores remotos en el seguimiento de la vegetación de dehesa y su influencia en el balance hidrológico a escala de cuenca

The Mediterranean region is characterized by hot summers with long dry periods, a situation that may be exacerbated by the progressive global warming. In these water-limited environments where productivity of the ecosystems depends mainly on water availability, the reduction of freshwater resources can have severe consequences. An increase in aridity may lead to low productivity, land degradation and unwanted changes in land use. To reduce the vulnerability of Mediterranean landscapes it is important to improve our knowledge of the hydrological processes conditioning the water exchanges, with evapotranspiration (ET) being a key indicator of the state of ecosystems and playing a crucial role in the basin's water and energy balances. The goal of this dissertation is to improve our understanding of the evapotranspiration dynamics over Mediterranean heterogeneous and complex vegetation covers, with a focus on the dehesa ecosystem. The final aim is to contribute to the conservation of the water resources in these regions in the medium to long term, supporting the decision-making processes with quantitative, distributed, and high-quality information. To reach this goal, in this research the evaluation of remote sensing-based soil water balance (SWB) and surface energy balance (SEB) models was proposed to monitor the water consumption and water stress of typical Mediterranean vegetation at different spatial and temporal scales. In particular, the VI-ETo methodology (SWB) and the ALEXI/DisALEXI approach (SEB) have been adapted and applied. ET modeling using the VI-ETo scheme has been improved through the assessment of the vegetation layers' effective parameters. A data fusion algorithm was applied to the ET maps produced by the SEB model over the dehesa ecosystem, and we analyzed the opportunities that this high-resolution ET product in time and space can provide for water and vegetation resource management. The results have demonstrated the feasibility of both approaches (SWB and SEB models) to accurately monitor ET dynamics over the dehesa landscape, adequately reproducing the annual bimodal behavior and the response of the vegetation in periods of water deficit. The error obtained using the SWB approach (the VI-ETo method) was RMSE = 0.47 mm day-1 over the whole dehesa system (grass + trees) and over an open grassland. The monitoring of water stress for both systems with different canopy structure, using as a proxy the ET/ETo ratio, and the stress coefficient (Ks), was successful. Improvements on the specific spectral properties of oak trees and layer-specific parameters were included into the modeling. We also analyzed the influence of the spectral properties of oak trees and another typical Mediterranean tree canopy, the olive orchard, in the VI-ETo model. We found that the use of appropriate values of the parameter SAVImax (0.51 for oak trees and 0.57 for olive trees) had notable implications in the computation of ET and water stress, in contrast to using a generic value for Mediterranean crops (SAVImax= 0.75). The accuracy of this water balance-based approach was also evaluated over two heterogeneous Mediterranean basins, with a mosaic of holm oaks and grasslands, shrubs, coniferous plantations, and irrigated horticultural crops. The annual discharge flows of both watersheds, which were determined from the modeled ET data and using a simple surface water balance, were very similar to those obtained with the HBV hydrological model, and to the values measured at the outlet of one of the basins, corroborating the usefulness of the VI-ETo methodology on these vegetation types. On the other hand, the resulting ET series (30 m, daily) derived with the SEB approach (ALEXI/DisALEXI method) and the STARFM fusion algorithm provided an RMSE value of 0.67 mm day-1, which was considered an acceptable error for management purposes. This error was slightly lower compared to using simpler interpolation methods, probably due to the high temporal frequency and better spatial representation of the flux tower footprint of the fused time series. The analysis of ET patterns over small heterogeneous vegetated patches that form the dehesa structure revealed the importance of having fine resolution information at field scale to distinguish the water consumed by the different vegetation components, which influences the provision of many ecosystem services. For example, it was key for identifying phenology dates of grasslands, or understanding the hydrological functioning of riverside dense evergreen vegetation with high ET rates during the whole year, in contrast with the herbaceous areas. Accurately modeling these different behaviors of dehesa microclimates is useful to support farmers‘ management and provide recommendations tailored for each structural component and requirements.La región mediterránea se caracteriza por veranos calurosos con largos períodos sin precipitaciones, situación que puede agravarse con el progresivo calentamiento global. En estos ambientes donde la productividad de los ecosistemas depende principalmente de la disponibilidad de agua, la reducción de los recursos hídricos puede tener graves consecuencias. Un aumento de la aridez puede conducir a una baja productividad, degradación de la tierra y cambios no deseados en el uso del suelo. Para reducir la vulnerabilidad de las zonas mediterráneas es importante profundizar en el estudio de los procesos hidrológicos que condicionan los intercambios de agua, siendo la evapotranspiración (ET) un indicador clave del estado de los ecosistemas y jugando un papel crucial en los balances hídricos y energéticos de la cuenca. El objetivo de esta tesis es mejorar nuestro conocimiento sobre la dinámica de la evapotranspiración en cubiertas mediterráneas heterogéneas y complejas, con el foco en el ecosistema de dehesa. El objetivo final es contribuir a la conservación de los recursos hídricos de estas regiones en el medio-largo plazo, apoyando en los procesos de toma de decisiones con información cuantitativa, distribuida y de calidad. Para alcanzar este objetivo, en esta investigación se propuso evaluar modelos de balance de agua en el suelo (SWB) y balance de energía en superficie (SEB) basados en el uso de sensores remotos, para el seguimiento del consumo de agua y el estrés hídrico de la vegetación mediterránea a diferentes escalas espaciales y temporales. En particular, se ha adaptado y aplicado la metodología VI-ETo (SWB) y el enfoque ALEXI/DisALEXI (SEB). Se ha mejorado el modelado de ET utilizando el esquema VI-ETo mediante la evaluación de los parámetros efectivos de las capas de vegetación. Se aplicó un algoritmo de fusión de datos remotos a los mapas de ET generados por el modelo SEB sobre el ecosistema de dehesa, y estudiamos las oportunidades que este producto de ET con alta resolución espacial y temporal puede aportar en la gestión de los recursos hídricos y de los ecosistemas. Los resultados han demostrado la viabilidad de ambos enfoques (modelos SWB y SEB) para monitorear con precisión la dinámica de la ET sobre el ecosistema de dehesa, reproduciendo adecuadamente el comportamiento bimodal anual y la respuesta de la vegetación en períodos de déficit hídrico. El error obtenido usando el enfoque SWB (el método VI-ETo) fue RMSE = 0.47 mm día-1, tanto para el sistema dehesa (pasto + árboles) como para una zona de pastizal. El seguimiento del estrés hídrico para ambos sistemas con diferente estructura de vegetación, utilizando la relación ET/ETo y el coeficiente de estrés (Ks), fue satisfactorio. Se incluyeron en el modelado mejoras sobre las propiedades espectrales específicas de las encinas y los parámetros específicos de los diferentes estratos de vegetación. También analizamos la influencia de las propiedades espectrales de las encinas y otra cubierta mediterránea, el olivar, en el modelo VI-ETo. Encontramos que el uso de valores apropiados del parámetro SAVImax (0,51 para robles y 0,57 para olivos) tuvo un efecto significativo en la determinación del consumo de agua y estrés hídrico, en comparación con usar un valor genérico para cultivos mediterráneos (SAVImax = 0,75). La precisión de este enfoque basado en el balance hídrico también se evaluó en dos cuencas mediterráneas heterogéneas, con un mosaico de encinas y pastizales, arbustos, plantaciones de coníferas y cultivos hortícolas de regadío. Los caudales de descarga anual de ambas cuencas, determinados a partir de los datos de ET modelados y utilizando un balance hídrico superficial muy simple, fueron muy similares a los obtenidos con el modelo hidrológico HBV, y a los valores medidos en la salida de una de las cuencas, corroborando la utilidad de la metodología VI-ETo sobre estas formaciones vegetales. Por otra parte, la serie final de ET (30 m, diaria) derivada del enfoque SEB (método ALEXI/DisALEXI) y del algoritmo de fusión STARFM proporcionó un valor de RMSE de 0,67 mm día-1, considerado un error aceptable para fines de manejo. Este error fue ligeramente inferior a los obtenidos usando métodos de interpolación más simples, debido probablemente a la alta frecuencia temporal y una mejor representación espacial del footprint de la torre de medida de flujos en la serie temporal fusionada. El análisis de los patrones de la ET sobre pequeñas manchas de vegetación heterogéneas, que forman la estructura de la dehesa, reveló la importancia de tener información con alta resolución a escala de campo para distinguir el agua consumida por los diferentes componentes de la vegetación, que tienen influencia en el aprovisionamiento de muchos servicios ecosistémicos. Por ejemplo, fue clave para identificar ciertas fechas fenológicas de los pastizales, o entender el funcionamiento hidrológico de la vegetación densa de hoja perenne en zonas de ribera con altas tasas de ET durante todo el año, en comparación con zonas de especies herbáceas. Modelar con precisión estos comportamientos diferentes de los microclimas de la dehesa es útil para apoyar la gestión de los agricultores y ofrecer recomendaciones adaptadas a cada componente y necesidades estructurales

Unmixing-Based Fusion of Hyperspatial and Hyperspectral Airborne Imagery for Early Detection of Vegetation Stress

"© 2014 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.” Upon publication, authors are asked to include either a link to the abstract of the published article in IEEE Xplore®, or the article’s Digital Object Identifier (DOI).Many applications require a timely acquisition of high spatial and spectral resolution remote sensing data. This is often not achievable since spaceborne remote sensing instruments face a tradeoff between spatial and spectral resolution, while airborne sensors mounted on a manned aircraft are too expensive to acquire a high temporal resolution. This gap between information needs and data availability inspires research on using Remotely Piloted Aircraft Systems (RPAS) to capture the desired high spectral and spatial information, furthermore providing temporal flexibility. Present hyperspectral imagers on board lightweight RPAS are still rare, due to the operational complexity, sensor weight, and instability. This paper looks into the use of a hyperspectral-hyperspatial fusion technique for an improved biophysical parameter retrieval and physiological assessment in agricultural crops. First, a biophysical parameter extraction study is performed on a simulated citrus orchard. Subsequently, the unmixing-based fusion is applied on a real test case in commercial citrus orchards with discontinuous canopies, in which a more efficient and accurate estimation of water stress is achieved by fusing thermal hyperspatial and hyperspectral (APEX) imagery. Narrowband reflectance indices that have proven their effectiveness as previsual indicators of water stress, such as the Photochemical Reflectance Index (PRI), show a significant increase in tree water-stress detection when applied on the fused dataset compared to the original hyperspectral APEX dataset (R-2 = 0.62, p 0.1). Maximal R-2 values of 0.93 and 0.86 are obtained by a linear relationship between the vegetation index and the resp., water and chlorophyll, parameter content maps.This work was supported in part by the Belgian Science Policy Office in the frame of the Stereo II program (Hypermix project-SR/00/141), in part by the project Chameleon of the Flemish Agency for Innovation by Science and Technology (IWT), and in part by the Spanish Ministry of Science and Education (MEC) for the projects AGL2012-40053-C03-01 and CONSOLIDER RIDECO (CSD2006-67). The European Facility for Airborne Research EUFAR (www.eufar.net) funded the flight campaign (Transnational Access Project 'Hyper-Stress'). The work of D. S. Intrigliolo was supported by the Spanish Ministry of Economy and Competitiveness program "Ramon y Cajal."Delalieux, S.; Zarco-Tejada, PJ.; Tits, L.; Jiménez Bello, MÁ.; Intrigliolo Molina, DS.; Somers, B. (2014). Unmixing-Based Fusion of Hyperspatial and Hyperspectral Airborne Imagery for Early Detection of Vegetation Stress. IEEE Journal of Selected Topics in Applied Earth Observations and Remote Sensing. 7(6):2571-2582. https://doi.org/10.1109/JSTARS.2014.2330352S257125827