Variability in gene cassette patterns of class 1 and 2 integrons associated with multi drug resistance patterns in Staphylococcus aureus clinical isolates in Tehran-Iran

Background: To investigate antibiotic resistance, the occurrence and distribution of class 1 and 2 integrons in multidrug- resistant Staphylococcus aureus isolates from hospitals in Tehran, Iran. The isolates were examined for susceptibility to antimicrobial agents. The mecA gene, class 1 and 2 integrons were detected by PCR. Integrase positive strains were further analysed for the presence of resistance gene cassettes using specific primers and were sequenced. Results: Among 139S.aureus isolates, 109 (78.4 ) and 112 (80.5 ) strains were considered as multidrug resistant and mecA positive, respectively. Class 1 integrons and internal variable regions were found in 72.6 (101/139) and 97 (98/101) and class 2 integrons and variable regions also in 35.2 (49/139) and 65.3 (32/49) of S.aureus clinical isolates, respectively. Twelve distinct cassette arrays were found, containing genes encoding resistance to β-lactams, aminoglycosides, streptothricin, trimethoprim, chloramphenicol,a putative glucose dehydrogenase precursor and a protein with unknown function. Gene cassette arrays aadB, aadA2 and dhfrA1-sat2-aadA1 were common in S.aureus isolates. We detected a completely new gene cassettes which contained aadB, oxa2, aacA4, orfD-aacA4-catB8, aadB-catB3, orfD-aacA4 and aadB-aadA1-cmlA6 of class 1 and dhfrA1-sat2-aadA1, dhfrA11, dhfrA1-sat2 of class 2 integrons. Conclusions: This is the first study to report carriage of class 1 and 2 integrons and associated gene cassettes among in S.aureus isolates from Iran. © 2015 Mostafa et al

Exact Solution for the Distribution of Transmission Eigenvalues in a Disordered Wire and Comparison with Random-Matrix Theory

An exact solution is presented of the Fokker-Planck equation which governs the evolution of an ensemble of disordered metal wires of increasing length, in a magnetic field. By a mapping onto a free-fermion problem, the complete probability distribution function of the transmission eigenvalues is obtained. The logarithmic eigenvalue repulsion of random-matrix theory is shown to break down for transmission eigenvalues which are not close to unity. ***Submitted to Physical Review B.****Comment: 20 pages, REVTeX-3.0, INLO-PUB-931028

Epstein-Barr virus transcription factor Zta acts through distal regulatory elements to directly control cellular gene expression

Lytic replication of the human gamma herpes virus Epstein-Barr virus (EBV) is an essential prerequisite for the spread of the virus. Differential regulation of a limited number of cellular genes has been reported in B-cells during the viral lytic replication cycle. We asked whether a viral bZIP transcription factor, Zta (BZLF1, ZEBRA, EB1), drives some of these changes. Using genome-wide chromatin immunoprecipitation coupled to next-generation DNA sequencing (ChIP-seq) we established a map of Zta interactions across the human genome. Using sensitive transcriptome analyses we identified 2263 cellular genes whose expression is significantly changed during the EBV lytic replication cycle. Zta binds 278 of the regulated genes and the distribution of binding sites shows that Zta binds mostly to sites that are distal to transcription start sites. This differs from the prevailing view that Zta activates viral genes by binding exclusively at promoter elements. We show that a synthetic Zta binding element confers Zta regulation at a distance and that distal Zta binding sites from cellular genes can confer Zta-mediated regulation on a heterologous promoter. This leads us to propose that Zta directly reprograms the expression of cellular genes through distal elements

Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

The question whether taxonomic descriptions naming new animal species without type specimen(s) deposited in collections should be accepted for publication by scientific journals and allowed by the Code has already been discussed in Zootaxa (Dubois & Nemésio 2007; Donegan 2008, 2009; Nemésio 2009a–b; Dubois 2009; Gentile & Snell 2009; Minelli 2009; Cianferoni & Bartolozzi 2016; Amorim et al. 2016). This question was again raised in a letter supported by 35 signatories published in the journal Nature (Pape et al. 2016) on 15 September 2016. On 25 September 2016, the following rebuttal (strictly limited to 300 words as per the editorial rules of Nature) was submitted to Nature, which on 18 October 2016 refused to publish it. As we think this problem is a very important one for zoological taxonomy, this text is published here exactly as submitted to Nature, followed by the list of the 493 taxonomists and collection-based researchers who signed it in the short time span from 20 September to 6 October 2016

Evaluating of Nitrate removal by adsorption/Fe/H2O2 process from water: Kinetics and operation parameters

Background: Today, global concerns about nitrate in groundwater and its adverse impact on health have increased. This study aimed to evaluate the efficiency of Nitrate reduction by Fe/H2O2 process and adsorption on the activated carbon. Materials and Methods: In this experimental-laboratory study, Nitrate oxidation by the advanced oxidation process Fe°/FeІІ/FeШ/H2O2 at pH 2-10, contact time 10-90 min, Nitrate concentrations of 50-300 mg/L was considered. A spectrophotometer was used to measure Nitrate in the effluent. Results: Results showed that the optimal parameters in the Fenton process in order to remove nitrate include: pH 3 and the contact time 15 min. By applying these conditions, the Nitrate removal efficiency in the retention time (90 min), initial nitrate concentration in 100 mg/L, iron concentration 10 mg/L and pH 4 for FeШ، FeІІ، Fe°، FeІІ/Fe°/H2O2 and FeШ/Fe°/H2O2 were 10.5%, 27.6%, 36.5%, 62.3% and 74%, respectively. Conclusion: According to the results, it was determined that the corrective fenton process with zero iron nano-particles can effectively reduce Nitrate under optimal conditions and this method can be used for the removal of similar compounds. Moreover, it was determined that the use of iron compounds in the presence of hydrogen peroxide can have a desirable effect on the decomposition of Nitrate and this method can be used for the removal of similar compounds