Genetic diversity in terrestrial subsurface ecosystems impacted by geological degassing

Earth’s mantle releases 38.7 ± 2.9 Tg/yr CO2 along with other reduced and oxidized gases to the atmosphere shaping microbial metabolism at volcanic sites across the globe, yet little is known about its impact on microbial life under non-thermal conditions. Here, we perform comparative metagenomics coupled to geochemical measurements of deep subsurface fluids from a cold-water geyser driven by mantle degassing. Key organisms belonging to uncultivated Candidatus Altiarchaeum show a global biogeographic pattern and site-specific adaptations shaped by gene loss and inter-kingdom horizontal gene transfer. Comparison of the geyser community to 16 other publicly available deep subsurface sites demonstrate a conservation of chemolithoautotrophic metabolism across sites. In silico replication measures suggest a linear relationship of bacterial replication with ecosystems depth with the exception of impacted sites, which show near surface characteristics. Our results suggest that subsurface ecosystems affected by geological degassing are hotspots for microbial life in the deep biosphere

Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection are often non-specific, and there is no definitive test for the accurate diagnosis of infection. The 'omics' approaches to identifying biomarkers from the host-response to bacterial infection are promising. In this study, lipidomic analysis was carried out with plasma samples obtained from febrile children with confirmed bacterial infection (n = 20) and confirmed viral infection (n = 20). We show for the first time that bacterial and viral infection produces distinct profile in the host lipidome. Some species of glycerophosphoinositol, sphingomyelin, lysophosphatidylcholine and cholesterol sulfate were higher in the confirmed virus infected group, while some species of fatty acids, glycerophosphocholine, glycerophosphoserine, lactosylceramide and bilirubin were lower in the confirmed virus infected group when compared with confirmed bacterial infected group. A combination of three lipids achieved an area under the receiver operating characteristic (ROC) curve of 0.911 (95% CI 0.81 to 0.98). This pilot study demonstrates the potential of metabolic biomarkers to assist clinicians in distinguishing bacterial from viral infection in febrile children, to facilitate effective clinical management and to the limit inappropriate use of antibiotics

Current Wildland Fire Patterns and Challenges in Europe: A Synthesis of National Perspectives

Changes in climate, land use, and land management impact the occurrence and severity of wildland fires in many parts of the world. This is particularly evident in Europe, where ongoing changes in land use have strongly modified fire patterns over the last decades. Although satellite data by the European Forest Fire Information System provide large-scale wildland fire statistics across European countries, there is still a crucial need to collect and summarize in-depth local analysis and understanding of the wildland fire condition and associated challenges across Europe. This article aims to provide a general overview of the current wildland fire patterns and challenges as perceived by national representatives, supplemented by national fire statistics (2009–2018) across Europe. For each of the 31 countries included, we present a perspective authored by scientists or practitioners from each respective country, representing a wide range of disciplines and cultural backgrounds. The authors were selected from members of the COST Action “Fire and the Earth System: Science & Society” funded by the European Commission with the aim to share knowledge and improve communication about wildland fire. Where relevant, a brief overview of key studies, particular wildland fire challenges a country is facing, and an overview of notable recent fire events are also presented. Key perceived challenges included (1) the lack of consistent and detailed records for wildland fire events, within and across countries, (2) an increase in wildland fires that pose a risk to properties and human life due to high population densities and sprawl into forested regions, and (3) the view that, irrespective of changes in management, climate change is likely to increase the frequency and impact of wildland fires in the coming decades. Addressing challenge (1) will not only be valuable in advancing national and pan-European wildland fire management strategies, but also in evaluating perceptions (2) and (3) against more robust quantitative evidence

Life-threatening infections in children in Europe (the EUCLIDS Project): a prospective cohort study

Background: Sepsis and severe focal infections represent a substantial disease burden in children admitted to hospital. We aimed to understand the burden of disease and outcomes in children with life-threatening bacterial infections in Europe. Methods: The European Union Childhood Life-threatening Infectious Disease Study (EUCLIDS) was a prospective, multicentre, cohort study done in six countries in Europe. Patients aged 1 month to 18 years with sepsis (or suspected sepsis) or severe focal infections, admitted to 98 participating hospitals in the UK, Austria, Germany, Lithuania, Spain, and the Netherlands were prospectively recruited between July 1, 2012, and Dec 31, 2015. To assess disease burden and outcomes, we collected demographic and clinical data using a secured web-based platform and obtained microbiological data using locally available clinical diagnostic procedures. Findings: 2844 patients were recruited and included in the analysis. 1512 (53·2%) of 2841 patients were male and median age was 39·1 months (IQR 12·4–93·9). 1229 (43·2%) patients had sepsis and 1615 (56·8%) had severe focal infections. Patients diagnosed with sepsis had a median age of 27·6 months (IQR 9·0–80·2), whereas those diagnosed with severe focal infections had a median age of 46·5 months (15·8–100·4; p<0·0001). Of 2844 patients in the entire cohort, the main clinical syndromes were pneumonia (511 [18·0%] patients), CNS infection (469 [16·5%]), and skin and soft tissue infection (247 [8·7%]). The causal microorganism was identified in 1359 (47·8%) children, with the most prevalent ones being Neisseria meningitidis (in 259 [9·1%] patients), followed by Staphylococcus aureus (in 222 [7·8%]), Streptococcus pneumoniae (in 219 [7·7%]), and group A streptococcus (in 162 [5·7%]). 1070 (37·6%) patients required admission to a paediatric intensive care unit. Of 2469 patients with outcome data, 57 (2·2%) deaths occurred: seven were in patients with severe focal infections and 50 in those with sepsis. Interpretation: Mortality in children admitted to hospital for sepsis or severe focal infections is low in Europe. The disease burden is mainly in children younger than 5 years and is largely due to vaccine-preventable meningococcal and pneumococcal infections. Despite the availability and application of clinical procedures for microbiological diagnosis, the causative organism remained unidentified in approximately 50% of patients

Plasma lipid profiles discriminate bacterial from viral infection in febrile children

Fever is the most common reason that children present to Emergency Departments. Clinical signs and symptoms suggestive of bacterial infection ar

Partial evaluation via code generation for static stochastic reaction network models (Software Appendix): [research data]

Succinct, declarative, and domain-specific modeling languages have many advantages when creating simulation models. However, it is often challenging to efficiently execute models defined in such languages. We use code generation for model-specific simulators. Code generation has been successfully applied for high-performance algorithms in many application domains. By generating tailored simulators for specific simulation models defined in a domain specific language, we get the best of both worlds: a succinct, declarative and formal presentation of the model and an efficient execution. We illustrate this based on a simple domain-specific language for biochemical reaction networks as well as on the network representation of the established BioNetGen language. We implement two approaches adopting the same simulation algorithms: one generic simulator that parses models at runtime and one generator that produces a simulator specialized to a given model based on partial evaluation and code generation. Akin to profile-guided optimization we also use dynamic execution of the model to further optimize the simulators. The performance of the approaches is carefully benchmarked using representative models of small to mid-sized biochemical reaction networks. The generic simulator achieves a performance similar to state of the art simulators in the domain, whereas the specialized simulator outperforms established simulation algorithms with a speedup of more than an order of magnitude. This repository contains the code generation software as described in the 2020 PADS paper Partial evaluation via code generation for static stochastic reaction network models

"Keile fehlt euch" - Gewalt im Medienverbund 'Revolte im Erziehungshaus' von Peter Martin Lampel

Josting P. "Keile fehlt euch" - Gewalt im Medienverbund 'Revolte im Erziehungshaus' von Peter Martin Lampel. In: Baader MS, Köster T, Schumann D, eds. Jugend - Gewalt. Erleben - Erörtern - Erinnern. Jugendbewegung und Jugendkulturen – Jahrbuch 2023. Vol 18. 1st ed. Göttingen: V&amp;R unipress; 2023: 127-143

A fast embedded language for continuous-time agent-based simulation

In agent-based simulation methods and applications, discrete timestep approaches prevail. To support continuous-time agent-based simulation, we analyze how methods for simulating population-based Con-tinu-ous-Time Markov Chains (CMTCs) can be adopted and derive implications for the concrete realization. To corroborate our findings, we develop an efficient internal domain-specific language (DSL) based on ML3, a modeling language for linked lives in demography. The design as an internal DSL, implemented within the Rust programming language, allows the modeler to exploit the complete feature set of the host language, such as data types and structures, when programming decision processes. A concise and expressive modeling of an agent's discrete decisions and behavior introducing exponentially distributed sojourn times can be supported by adapting the concept of guarded commands from population-based CTMCs. The execution of models relies on an optimized version of the direct method. This method is a variant of stochastic simulation algorithms, an established method for executing population-based CTMCs in other application areas, notably biochemistry. To efficiently handle the large set of possible transitions inherent to continuous-time agent-based models, we use a dependency graph whose updating scheme caters to the dynamic dependencies within agent-based models and the need for efficient implementation. The presented case studies include implementations of a continuous-time, agent-based migration model and a comparative performance study based on an extended SIR model of infection spread, allowing us to draw conclusions about the impact of different design choices on efficiency

varlociraptor/varlociraptor: v8.4.2

&lt;h2&gt;&lt;a href="https://github.com/varlociraptor/varlociraptor/compare/v8.4.1...v8.4.2"&gt;8.4.2&lt;/a&gt; (2023-11-09)&lt;/h2&gt; &lt;h3&gt;Bug Fixes&lt;/h3&gt; &lt;ul&gt; &lt;li&gt;out of bounds error in estimation of third allele probability (&lt;a href="https://github.com/varlociraptor/varlociraptor/issues/403"&gt;#403&lt;/a&gt;) (&lt;a href="https://github.com/varlociraptor/varlociraptor/commit/37eafa402865a9aa84d818233dc24dfeb301270a"&gt;37eafa4&lt;/a&gt;)&lt;/li&gt; &lt;/ul&gt