Changes in Brain Biochemistry and Feeding Behavior in Hybrid Striped Bass Exposed to Environmental Contaminants

Biochemical endpoints are useful when determining contaminant exposure in aquatic organisms. However, behaviors can also be impaired by environmental contaminants. Links between changes in brain biochemistry and behavior have been made, and understanding these relationships could increase the utility of these endpoints in ecological risk assessments. The overall goal of this research was to determine relationships between an ecologically relevant fish behavior and brain biochemistry. To accomplish this goal, I developed a behavioral bioassay that quantified the time it took exposed hybrid striped bass (Morone saxatilis x M. chrysops) to capture unexposed prey, fathead minnows (Pimephales promelas). Chemically targeted brain neurotransmitters were also monitored, and the relationship with behavior was determined. Six-day acute exposures to (1) a pesticide (diazinon) targeting acetylcholinesterase (AChE) and (2) a pharmaceutical (fluoxetine) targeting serotonin were conducted with a 6-day recovery periods. Brain biochemistry and behaviors were plotted against each other. Our results indicated that there was a threshold response between AChE activity and feeding behavior following diazinon exposure concentrations of 19.1 ± 0.7, 64.0 ± 2.0, and 101.9 ± 1.4 µg/l. By day 6, AChE activity was significantly inhibited in the low, medium, and high treatment groups by 66.3, 82.2, 86.4%, respectively. However, there were no signs of behavioral impairment in the lowest treatment group. During the 6-day recovery period, there were concentration- and duration-dependent changes in feeding behavior and AChE activity, in which time to capture prey decreased more rapidly than AChE activity increased. Following fluoxetine exposures (23.2 ± 6.6, 51.4 ± 10.9, and 100.9 ± 18.6 µg/l), a linear response between decreased serotonin activity and increased feeding behavior was observed. However, maximum serotonin depression in the low, medium, and high treatment groups occurred on day 9 (day 3 of the recovery period) with concentrations at 23.7, 28.0, and 49.1% of controls, respectively. Our results also indicated that during the recovery period, there was a concentration- and duration-dependent increase in serotonin activity accompanied by a decrease in time to capture prey. A 27-day chronic exposure to fluoxetine was also conducted at lower exposure concentrations (0.08 ± 0.02, 0.87 ± 0.12, 9.44 ± 0.82 µg/l) than the acute exposure. It was concluded that although fluoxetine can cause impaired serotonin levels and feeding behavior, this was not observed at more environmentally relevant concentrations over the 27 days

Extraction of Multi-layered Social Networks from Activity Data

The data gathered in all kind of web-based systems, which enable users to interact with each other, provides an opportunity to extract social networks that consist of people and relationships between them. The emerging structures are very complex due to the number and type of discovered connections. In webbased systems, the characteristic element of each interaction between users is that there is always an object that serves as a communication medium. This can be e.g. an email sent from one user to another or post at the forum authored by one user and commented by others. Based on these objects and activities that users perform towards them, different kinds of relationships can be identified and extracted. Additional challenge arises from the fact that hierarchies can exist between objects, e.g. a forum consists of one or more groups of topics, and each of them contains topics that finally include posts. In this paper, we propose a new method for creation of multi-layered social network based on the data about users activities towards different types of objects between which the hierarchy exists. Due to the flattening, preprocessing procedure new layers and new relationships in the multi-layered social network can be identified and analysed.Comment: 20 pages, 15 figure

The read-across hypothesis and environmental risk assessment of pharmaceuticals

This article is made available through the Brunel Open Access Publishing Fund. Copyright © 2013 American Chemical Society.Pharmaceuticals in the environment have received increased attention over the past decade, as they are ubiquitous in rivers and waterways. Concentrations are in sub-ng to low μg/L, well below acute toxic levels, but there are uncertainties regarding the effects of chronic exposures and there is a need to prioritise which pharmaceuticals may be of concern. The read-across hypothesis stipulates that a drug will have an effect in non-target organisms only if the molecular targets such as receptors and enzymes have been conserved, resulting in a (specific) pharmacological effect only if plasma concentrations are similar to human therapeutic concentrations. If this holds true for different classes of pharmaceuticals, it should be possible to predict the potential environmental impact from information obtained during the drug development process. This paper critically reviews the evidence for read-across, and finds that few studies include plasma concentrations and mode of action based effects. Thus, despite a large number of apparently relevant papers and a general acceptance of the hypothesis, there is an absence of documented evidence. There is a need for large-scale studies to generate robust data for testing the read-across hypothesis and developing predictive models, the only feasible approach to protecting the environment.BBSRC Industrial Partnership Award BB/ I00646X/1 and BBSRC Industrial CASE Partnership Studentship BB/I53257X/1 with AstraZeneca Safety Health and Environment Research Programme

Pharmaceutical Formulation Facilities as Sources of Opioids and Other Pharmaceuticals to Wastewater Treatment Plant Effluents

Facilities involved in the manufacture of pharmaceutical products are an under-investigated source of pharmaceuticals to the environment. Between 2004 and 2009, 35 to 38 effluent samples were collected from each of three wastewater treatment plants (WWTPs) in New York and analyzed for seven pharmaceuticals including opioids and muscle relaxants. Two WWTPs (NY2 and NY3) receive substantial flows (>20% of plant flow) from pharmaceutical formulation facilities (PFF) and one (NY1) receives no PFF flow. Samples of effluents from 23 WWTPs across the United States were analyzed once for these pharmaceuticals as part of a national survey. Maximum pharmaceutical effluent concentrations for the national survey and NY1 effluent samples were generally <1 μg/L. Four pharmaceuticals (methadone, oxycodone, butalbital, and metaxalone) in samples of NY3 effluent had median concentrations ranging from 3.4 to >400 μg/L. Maximum concentrations of oxycodone (1700 μg/L) and metaxalone (3800 μg/L) in samples from NY3 effluent exceeded 1000 μg/L. Three pharmaceuticals (butalbital, carisoprodol, and oxycodone) in samples of NY2 effluent had median concentrations ranging from 2 to 11 μg/L. These findings suggest that current manufacturing practices at these PFFs can result in pharmaceuticals concentrations from 10 to 1000 times higher than those typically found in WWTP effluents

Transcriptome sequencing and microarray development for the Manila clam, Ruditapes philippinarum: genomic tools for environmental monitoring

Abstract Background The Manila clam, Ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems. However, genomic resources for R. philippinarum are still extremely limited. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various environmental stressors on aquatic animals under either artificial conditions or in the wild. Here, we report on the development of a transcriptomic platform for global gene expression profiling in the Manila clam. Results A normalized cDNA library representing a mixture of adult tissues was sequenced using a ultra high-throughput sequencing technology (Roche 454). A database consisting of 32,606 unique transcripts was constructed, 9,747 (30%) of which could be annotated by similarity. An oligo-DNA microarray platform was designed and applied to profile gene expression of digestive gland and gills. Functional annotation of differentially expressed genes between different tissues was performed by enrichment analysis. Expression of Natural Antisense Transcripts (NAT) analysis was also performed and bi-directional transcription appears a common phenomenon in the R. philippinarum transcriptome. A preliminary study on clam samples collected in a highly polluted area of the Venice Lagoon demonstrated the applicability of genomic tools to environmental monitoring. Conclusions The transcriptomic platform developed for the Manila clam confirmed the high level of reproducibility of current microarray technology. Next-generation sequencing provided a good representation of the clam transcriptome. Despite the known limitations in transcript annotation and sequence coverage for non model species, sufficient information was obtained to identify a large set of genes potentially involved in cellular response to environmental stress.This work was partially supported by a grant from European Union-funded Network of Excellence "Marine Genomics Europe". CS wishes to acknowledge additional funding from the Ministry of Education and Science (Spain) through grant AGL2007-60049. MM had a PhD scholarship from the University of Florence, Italy. RL was recipient of PhD fellowship SFRH/BD/30112/2006, from the Portuguese Science and Technology Foundation (FCT) and LC and RL acknowledge a grant from FCT project ISOPERK (PTDC/CVT/72083/2006).Peer Reviewe

Fundulus as the premier teleost model in environmental biology : opportunities for new insights using genomics

Author Posting. © Elsevier B.V., 2007. This is the author's version of the work. It is posted here by permission of Elsevier B.V. for personal use, not for redistribution. The definitive version was published in Comparative Biochemistry and Physiology Part D: Genomics and Proteomics 2 (2007): 257-286, doi:10.1016/j.cbd.2007.09.001.A strong foundation of basic and applied research documents that the estuarine fish Fundulus heteroclitus and related species are unique laboratory and field models for understanding how individuals and populations interact with their environment. In this paper we summarize an extensive body of work examining the adaptive responses of Fundulus species to environmental conditions, and describe how this research has contributed importantly to our understanding of physiology, gene regulation, toxicology, and ecological and evolutionary genetics of teleosts and other vertebrates. These explorations have reached a critical juncture at which advancement is hindered by the lack of genomic resources for these species. We suggest that a more complete genomics toolbox for F. heteroclitus and related species will permit researchers to exploit the power of this model organism to rapidly advance our understanding of fundamental biological and pathological mechanisms among vertebrates, as well as ecological strategies and evolutionary processes common to all living organisms.This material is based on work supported by grants from the National Science Foundation DBI-0420504 (LJB), OCE 0308777 (DLC, RNW, BBR), BES-0553523 (AW), IBN 0236494 (BBR), IOB-0519579 (DHE), IOB-0543860 (DWT), FSML-0533189 (SC); National Institute of Health NIEHS P42-ES007381(GVC, MEH), P42-ES10356 (RTD), ES011588 (MFO); and NCRR P20 RR-016463 (DWT); Natural Sciences and Engineering Research Council of Canada Discovery (DLM, TDS, WSM) and Collaborative Research and Development Programs (DLM); NOAA/National Sea Grant NA86RG0052 (LJB), NA16RG2273 (SIK, MEH,GVC, JJS); Environmental Protection Agency U91620701 (WSB), R82902201(SC) and EPA’s Office of Research and Development (DEN)