Squamscott Project Stratham Ad Hoc Conservation Bond Subcommittee to the Conservation Committee

Through the Stratham Ad Hoc Conservation Bond Subcommittee to the Conservation Committee (Ad Hoc Committee) the objectives under the Squamscott Project were to educate the citizens of Stratham on conservation options and estate planning, and to create a natural resource prioritization and criteria ranking for critical areas in town in need of protection efforts. The Ad Hoc Committee contracted with the Rockingham County Conservation District to assist with these efforts and to provide assistance with the preparation of funding applications to leverage the existing open space bond fund

Squamscott Project Newfields Open Space Committee

Through the Newfields Open Space Committee (NOSC) the objectives under the Squamscott Project were to educate the citizens of Newfields on conservation options and estate planning, the goals and objectives of the NOSC, and to finalize a natural resource prioritization for critical areas in town in need of protection efforts. The NOSC contracted with the Rockingha m County Conservation District to assist with these efforts, and to provide assistance with the coordination of conservation projects and the preparation of funding applications to leverage the existing open space bond fund

On the minimization of the prime power consumption of a coupling-modulated gas laser transmitter

The prime power requirements of a coupling-modulated gas laser transmitter are presented. The latter consists of a gas discharge tube and electro-optic modulator inside a laser resonator. In performing the calculations, the laser discharge length and the modulator voltage are simultaneously varied so that the transmitted power remains constant. In this way, tradeoffs can be made between the prime power supplied individually to the discharge tube and to the modulator driver to obtain a transmitter configuration which minimizes the total prime power consumption. An analytical expression is derived which describes the effects of information bandwidth and transmitter output power on the prime power requirements. Specific numerical results are obtained for a CO2 laser transmitter based on presently available experimental data

A polynomial time algorithm for calculating the probability of a ranked gene tree given a species tree

In this paper, we provide a polynomial time algorithm to calculate the probability of a {\it ranked} gene tree topology for a given species tree, where a ranked tree topology is a tree topology with the internal vertices being ordered. The probability of a gene tree topology can thus be calculated in polynomial time if the number of orderings of the internal vertices is a polynomial number. However, the complexity of calculating the probability of a gene tree topology with an exponential number of rankings for a given species tree remains unknown

Thiamine Acquisition Strategies Impact Metabolism and Competition in the Gut Microbe Bacteroides thetaiotaomicron.

Thiamine (vitamin B1) is an essential cofactor for all organisms. Humans primarily acquire thiamine through their diet, and thiamine deficiencies have adverse neurological effects. However, the role gut microbes play in modulating thiamine availability is poorly understood, and little is known about how thiamine impacts the stability of microbial gut communities. To investigate thiamine's role in the gut, we utilized the model gut microbe Bacteroides thetaiotaomicron. Transcriptome sequencing (RNA-seq) revealed a global downregulation of thiamine and amino acid biosynthesis, glycolysis, and purine metabolism when thiamine was present. Using genetic mutants with thiamine biosynthesis and transport locus mutations, we determined both systems were critical for growth in thiamine-deficient medium. The defect in the double transport mutant suggests an uncharacterized feedback mechanism between thiamine transport and biosynthesis in B. thetaiotaomicron. Mutant phenotypes were recapitulated during pairwise competitions, reinforcing the importance of encoding versatile thiamine acquisition mechanisms when thiamine concentrations are variable. In addition, liquid chromatography-mass spectrometry (LC-MS) analyses corroborate that exogenous thiamine levels affect the internal thiamine pool of B. thetaiotaomicron. Furthermore, we computationally examined the ability of other gut microbes to acquire thiamine and identified lineage-specific differences in thiamine acquisition strategies. Among the Bacteroidetes, the capacities for both thiamine transport and biosynthesis are common. Together, these data show that thiamine acquisition mechanisms used by B. thetaiotaomicron not only are critical for its physiology and fitness but also provide the opportunity to model how other gut microbes may respond to the shifting availability of thiamine in the gut. IMPORTANCE Variation in the ability of gut microbes to transport, synthesize, and compete for vitamin B1 (thiamine) is expected to impact the structure and stability of the microbiota, and ultimately this variation may have both direct and indirect effects on human health. Our study identifies the diverse strategies employed by gut Bacteroidetes to acquire thiamine. We demonstrate how the presence or absence of thiamine biosynthesis or transport dramatically affects the abundance of B. thetaiotaomicron in a competitive environment. This study adds further evidence that altering the presence or concentrations of water-soluble vitamins such as thiamine may be an effective method for manipulating gut community composition. In turn, targeted thiamine delivery could be used therapeutically to alter dysbiotic communities linked to disease. Author Video: An author video summary of this article is available