Endocrine disruption in wildlife: The future?

Probably the only thing that can be said with certainty about the future of this field of ecotoxicology is that predicting it is foolish; the chances of being right are very slim. Instead, it seems to me likely that unexpected discoveries will probably have more influence on the field of endocrine disruption than the outcomes of all the planned experiments. It is certainly true that chance discoveries, such as masculinized fish in rivers receiving paper-mill effluent, imposex in molluscs due to exposure to tributyltin and feminized fish in rivers receiving effluent from sewage-treatment works, have been pivotal in the development of the field of endocrine disruption in wildlife. I consider that further such discoveries are likely, but I do not know which species will be affected, what effects will be found, what chemical(s) will be the cause, or what endocrine mechanism(s) will underlie the effects. The recent realization that many pharmaceuticals are present in the aquatic environment only underscores the range of effects that could, in theory at least, occur in exposed wildlife. What is somewhat easier to predict is the research that will be conducted in the immediate future, which will build upon what is known already. For example, it is clear that wildlife is rarely, if ever, exposed to single chemicals, but instead is exposed to highly complex, ill-defined mixtures of chemicals, including many that are endocrine active in various ways. We need to understand much better how chemicals interact, and what overall effects will occur upon exposure to such mixtures. We also need to move from assessing effects at the individual organism level, to understanding the consequences of these effects at the population level. Then, we need to determine the significance of any population-level effects due to endocrine disruption in comparison with the impact of many other significant stressors (e.g., over-exploitation, habitat loss, climate change) that also negatively impact wildlife. Such research will be difficult, and time-consuming, and will probably produce many surprises. All I can be fairly certain about is that the next few years are likely to be as interesting and exciting as the last few have been

RESEARCH EXCHANGE. Reactive Attachment Disorder: Developing a Developmental Perspective

Reactive Attachment Disorder is a relatively young disorder. Researchers are just beginning to hash out the implications of this disorder on current children and future generations. However, there is much needed from criteria setting and researching leadership to mediate the process of gaining ground in assessing and treating this disorder. This meta-­‐analysis will provide an overview that will point out the diagnostic ambiguities, theoretical conflicts, and disjointed research of the previous decade’s work on RAD

Structural features of alkylphenolic chemicals associated with estrogenic activity

The ability of certain man-made chemicals to mimic the effects of natural steroid hormones and their potential to disrupt the delicate balance of the endocrine system in animals are of increasing concern. The growing list of reported hormone-mimics includes the alkylphenolic (AP) compounds, a small number of which have been reported to be weakly estrogenic. In their most basic form, APs are composed of an alkyl group, which can vary in size, branching, and position, joined to a phenolic ring. The aim of this project was to identify the important structural features responsible for the estrogenic activity of AP chemicals. This was achieved by incubating APs with different structural features in a medium containing a previously described estrogen-inducible strain of yeast (Saccharomyces cerevisiae) expressing the human estrogen receptor and comparing their activity spectrophotometrically by the resulting color change of the medium. The results were compared to the effects of the main natural estrogen 17-estradiol. The data indicate that both the position (para > meta > ortho) and branching (tertiary > secondary = normal) of the alkyl group affect estrogenicity. Optimal estrogenic activity requires a single tertiary branched alkyl group composed of between 6 and 8 carbons located at the para position on an otherwise unhindered phenol ring. The results are discussed in relation to the purity and composition of the chemicals tested

Mixtures of Chemicals in Water: Implications of chemical legislation and environmental policy

Scientists have shown that mixtures of chemicals can act together to reduce the reproductive capacity of fish even if each individual chemical in the mixture is present at a concentration that does not cause an adverse effect. This evidence demonstrates that legislation based on the assessment of single chemicals may not be sufficiently protective and suggest that there is a need for a review of existing environmental policy

Laser-initiated ordnance for air-to-air missiles

McDonnell Douglas Missile Systems Company (MDMSC) has developed a laser ignition subsystem (LIS) for air-to-air missile applications. The MDMSC subsystem is designed to activate batteries, unlock fins, and sequence propulsion system events. The subsystem includes Pyro Zirconium Pump (PZP) lasers, mechanical Safe & Arm, fiber-optic distribution system, and optically activated pyrotechnic devices (initiators, detonators, and thermal batteries). The LIS design has incorporated testability features for the laser modules, drive electronics, fiber-optics, and pyrotechnics. Several of the LIS have been fabricated and have supported thermal battery testing, integral rocket ramjet testing, and have been integrated into integral rocket ramjet flight test vehicles as part of the flight control subsystem

Seasonal changes in serum testosterone, 11-ketotestosterone, and 1713-estradiol levels in the brown bullhead, Ictalurus nebuIosus Lesueur

The seasonal changes in gonadosomatic index (GSI) and strum testosterone, I I-ketotestosterone, and 17&estmdiol levels were measured in adult feral brown bullheads, Ictalurus nebulosus Lzsueur. The maximum GSI of both male and femalz brown bullheads was consjderably lower than that of most other teleostean species investigated. lo males, the GSI began to increase in April concomitant with an increase in water temperature from 3 to 6°C. The maximum GSI levels were evident throu_ghout May and June (during the prespawning and spawning periods). Peaks of serum testosterone and serum 11-ketotesrosterone leveb were evident in mid-April and late May to June, and in mid-April and mid-May, respxtively. In females here was a rapid increase in GSI during May, when the ambient water temperamre reached 16°C. The peak GSI was evident in mid- to late-May and had declined by early June. Peak serum testosterone and I 1-ketotestosterone levels were evident .in mid-April and again in late May. wbereas peak 17P-estradiol levels were fou.nd in mid-May and mid-June. The peak serum teswsterone levels in females were 4.5-fold higher than in the males, whereas che I I-ketotestosterone levels were similar in males and females

Perceptual outcomes as reinforcers

The paucity of basic behavioral research with humans was highlighted almost 40 years ago (Findley, 1966). A recent search of the basic behavioral research journals by the present authors indicated that this situation remains, with only approximately 15% of the research using human participants. It may seem surprising that so few studies use human participants given the apparent practical advantages of doing so. Human participants are readily available, extensively pre-trained, can use a range of complex apparatus and require no feeding or housing. In reality, however, the use of human participants involves a number of practical difficulties: humans generally will not agree to participate in research involving a large number of sessions or sessions lasting more than two to three hours; it is difficult to gain within-session compliance as a result of the often repetitive and simplistic nature of the tasks; and it is often unclear whether the experimental results are a product of the experimentally-arranged contingencies or the instructions given to the participants. Another difficulty, that which is addressed here, lies in the identification of a suitable experimental outcome that will serve as a reinforcer

Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment

A rapidly increasing number of chemicals, or their degradation products, are being recognized as possessing estrogenic activity, albeit usually weak. We have found that effluent from sewage treatment works contains a chemical, or mixture of chemicals, that induces vitellogenin synthesis in male fish maintained in the effluent, thus indicating that the effluent is estrogenic. The effect was extremely pronounced and occurred at all sewage treatment works tested. The nature of the chemical or chemicals causing the effect is presently not known. However, we have tested a number of chemicals known to be estrogenic to mammals and have shown that they are also estrogenic to fish; that is, no species specificity was apparent. Many of these weakly estrogenic chemicals are known to be present in effluents. Further, a mixture of different estrogenic chemicals was considerably more potent than each of the chemicals when tested individually, suggesting that enhanced effects could occur when fish are exposed simultaneously to various estrogenic chemicals (as is likely to occur in rivers receiving effluent). Subsequent work should determine whether exposure to these chemicals at the concentrations present in the environment leads to any deleterious physiological effects

Estrogenic activity of phenolic additives determined by an in vitro yeast bioassay

Copyright @ 2001 Environmental Health PerspectivesWe used a recombinant yeast estrogen assay to assess the activity of 73 phenolic additives that are used as sunscreens, preservatives, disinfectants, antioxidants, flavorings, or for perfumery. Thirty-two of these compounds displayed activity: 22 with potencies relative to 17 beta -estradiol, ranging from 1/3,000 to -estradiol. Forty-one compounds were inactive. The major criteria for activity appear to be the presence of an unhindered phenolic OH group in a para position and a molecular weight of 140-250 Da.This work was supported in part under contract with the U.K. Department of Trade and Industry as part of the Government Chemist Programme