HID-1 controls formation of large dense core vesicles by influencing cargo sorting and trans-Golgi network acidification

Large dense core vesicles (LDCVs) mediate the regulated release of neuropeptides and peptide hormones. They form at the trans-Golgi network (TGN), where their soluble content aggregates to form a dense core, but the mechanisms controlling biogenesis are still not completely understood. Recent studies have implicated the peripheral membrane protein HID-1 in neuropeptide sorting and insulin secretion. Using CRISPR/Cas9, we generated HID-1 KO rat neuroendocrine cells, and we show that the absence of HID-1 results in specific defects in peptide hormone and monoamine storage and regulated secretion. Loss of HID-1 causes a reduction in the number of LDCVs and affects their morphology and biochemical properties, due to impaired cargo sorting and dense core formation. HID-1 KO cells also exhibit defects in TGN acidification together with mislocalization of the Golgi-enriched vacuolar H+-ATPase subunit isoform a2. We propose that HID-1 influences early steps in LDCV formation by controlling dense core formation at the TGN.</jats:p

Concert recording 2018-04-08a

[Track 1]. Sonatina. 1. [Track 2]. 2. [Track 3]. 3. / Halsey Stevens -- [Track 4]. Songs of a wayfarer. I. Wenn mein Schatz Hochzeit macht [Track 5]. II. Ging heut Morgen uber\u27s Feld [Track 6]. III. Ich hab\u27 ein gluhend Messer [Track 7]. IV. Die Zwei Blauen Augen / Gustav Mahler -- [Track 8]. Sonata in F minor. I. Andante cantabile [Track 9]. II. Allegro [Track 10]. III. Andante / Georg Philipp Telemann -- [Track 11]. 10 pieces for two bass trombones. 1. The hitch hiker [Track 12]. 6. Cashmere [Track 13]. 8. Below 10 street / Tommy Pederson -- [Track 14]. Gospel time / Jeffrey Agrell

Thirty New Low-Mass Spectroscopic Binaries

As part of our search for young M dwarfs within 25 pc, we acquired high-resolution spectra of 185 low-mass stars compiled by the NStars project that have strong X-ray emission. By cross-correlating these spectra with radial velocity standard stars, we are sensitive to finding multi-lined spectroscopic binaries. We find a low-mass spectroscopic binary fraction of 16% consisting of 27 SB2s, 2 SB3s and 1 SB4, increasing the number of known low-mass SBs by 50% and proving that strong X-ray emission is an extremely efficient way to find M-dwarf SBs. WASP photometry of 23 of these systems revealed two low-mass EBs, bringing the count of known M dwarf EBs to 15. BD -22 5866, the SB4, is fully described in Shkolnik et al. 2008 and CCDM J04404+3127 B consists of a two mid-M stars orbiting each other every 2.048 days. WASP also provided rotation periods for 12 systems, and in the cases where the synchronization time scales are short, we used P_rot to determine the true orbital parameters. For those with no P_rot, we use differential radial velocities to set upper limits on orbital periods and semi-major axes. More than half of our sample has near-equal-mass components (q > 0.8). This is expected since our sample is biased towards tight orbits where saturated X-ray emission is due to tidal spin-up rather than stellar youth. Increasing the samples of M dwarf SBs and EBs is extremely valuable in setting constraints on current theories of stellar multiplicity and evolution scenarios for low-mass multiple systems.Comment: Accepted to Ap

Optical data of meteoritic nano-diamonds from far-ultraviolet to far-infrared wavelengths

We have used different spectroscopic techniques to obtain a consistent quantitative absorption spectrum of a sample of meteoritic nano-diamonds in the wavelength range from the vacuum ultraviolet (0.12 $\mu$m) to the far infrared (100 $\mu$m). The nano-diamonds have been isolated by a chemical treatment from the Allende meteorite (Braatz et al.2000). Electron energy loss spectroscopy (EELS) extends the optical measurements to higher energies and allows the derivation of the optical constants (n & k) by Kramers-Kronig analysis. The results can be used to restrain observations and to improve current models of the environment where the nano-diamonds are expected to have formed. We also show that the amount of nano-diamond which can be present in space is higher than previously estimated by Lewis et al. (1989).Comment: 11 pages, 7 figure

A multiwavelength study of Swift GRB 060111B constraining the origin of its prompt optical emission

In this work, we present the results obtained from a multi-wavelength campaign, as well as from the public Swift/BAT, XRT, and UVOT data of GRB 060111B for which a bright optical emission was measured with good temporal resolution during the prompt phase. We identified the host galaxy at R~25 mag; its featureless spectral continuum and brightness, as well as the non-detection of any associated supernova 16 days after the trigger and other independent redshift estimates, converge to z~1-2. From the analysis of the early afterglow SED, we find that non-negligible host galaxy dust extinction, in addition to the Galactic one, affects the observed flux in the optical regime. The extinction-corrected optical-to-gamma-ray spectral energy distribution during the prompt emission shows a flux density ratio $F_{\gamma}/F_{opt}$=0.01-0.0001 with spectral index $\beta_{\gamma,opt}> \beta_{\gamma}$, strongly suggesting a separate origin of the optical and gamma-ray components. This result is supported by the lack of correlated behavior in the prompt emission light curves observed in the two energy domains. The properties of the prompt optical emission observed during GRB 060111B favor interpretation of this optical light as radiation from the reverse shock in a thick shell limit and in the slow cooling regime. The expected peak flux is consistent with the observed one corrected for the host extinction, likely indicating that the starting time of the TAROT observations is very near to or coincident with the peak time. The estimated fireball initial Lorentz factor is >260-360 at z=1-2, similar to the Lorentz factors obtained from other GRBs. GRB 060111B is a rare, good test case of the reverse shock emission mechanism in both the X-ray and optical energy ranges.Comment: Accepted for publication in Astronomy and Astrophysics, 15 pages,10 figures and 7 table

Prostatic Acid Phosphatase Is Required for the Antinociceptive Effects of Thiamine and Benfotiamine

Peer reviewe

Calibration of the charge and energy loss per unit length of the MicroBooNE liquid argon time projection chamber using muons and protons

We describe a method used to calibrate the position- and time-dependent response of the MicroBooNE liquid argon time projection chamber anode wires to ionization particle energy loss. The method makes use of crossing cosmic-ray muons to partially correct anode wire signals for multiple effects as a function of time and position, including cross-connected TPC wires, space charge effects, electron attachment to impurities, diffusion, and recombination. The overall energy scale is then determined using fully-contained beam-induced muons originating and stopping in the active region of the detector. Using this method, we obtain an absolute energy scale uncertainty of 2% in data. We use stopping protons to further refine the relation between the measured charge and the energy loss for highly-ionizing particles. This data-driven detector calibration improves both the measurement of total deposited energy and particle identification based on energy loss per unit length as a function of residual range. As an example, the proton selection efficiency is increased by 2% after detector calibration

Reconstruction and measurement of (100) MeV energy electromagnetic activity from π0 arrow γγ decays in the MicroBooNE LArTPC

We present results on the reconstruction of electromagnetic (EM) activity from photons produced in charged current νμ interactions with final state π0s. We employ a fully-automated reconstruction chain capable of identifying EM showers of (100) MeV energy, relying on a combination of traditional reconstruction techniques together with novel machine-learning approaches. These studies demonstrate good energy resolution, and good agreement between data and simulation, relying on the reconstructed invariant π0 mass and other photon distributions for validation. The reconstruction techniques developed are applied to a selection of νμ + Ar → μ + π0 + X candidate events to demonstrate the potential for calorimetric separation of photons from electrons and reconstruction of π0 kinematics

A faint optical flash in dust-obscured GRB 080603A - implications for GRB prompt emission mechanisms

We report the detection of a faint optical flash by the 2-m Faulkes Telescope North simultaneously with the second of two prompt gamma-ray pulses in INTEGRAL gamma-ray burst (GRB) 080603A, beginning at t_rest = 37 s after the onset of the GRB. This optical flash appears to be distinct from the subsequent emerging afterglow emission, for which we present comprehensive broadband radio to X-ray light curves to 13 days post-burst and rigorously test the standard fireball model. The intrinsic extinction toward GRB 080603A is high (A_V,z = 0.8 mag), and the well-sampled X-ray-to-near-infrared spectral energy distribution is interesting in requiring an LMC2 extinction profile, in contrast to the majority of GRBs. Comparison of the gamma-ray and extinction-corrected optical flux densities of the flash rules out an inverse-Compton origin for the prompt gamma-rays; instead, we suggest that the optical flash could originate from the inhomogeneity of the relativistic flow. In this scenario, a large velocity irregularity in the flow produces the prompt gamma-rays, followed by a milder internal shock at a larger radius that would cause the optical flash. Flat gamma-ray spectra, roughly F propto nu^-0.1, are observed in many GRBs. If the flat spectrum extends down to the optical band in GRB 080603A, the optical flare could be explained as the low-energy tail of the gamma-ray emission. If this is indeed the case, it provides an important clue to understanding the nature of the emission process in the prompt phase of GRBs and highlights the importance of deep (R> 20 mag), rapid follow-up observations capable of detecting faint, prompt optical emission.Comment: 22 pages, 11 figures, accepted to MNRA

Design and construction of the MicroBooNE Cosmic Ray Tagger system

The MicroBooNE detector utilizes a liquid argon time projection chamber (LArTPC) with an 85 t active mass to study neutrino interactions along the Booster Neutrino Beam (BNB) at Fermilab. With a deployment location near ground level, the detector records many cosmic muon tracks in each beam-related detector trigger that can be misidentified as signals of interest. To reduce these cosmogenic backgrounds, we have designed and constructed a TPC-external Cosmic Ray Tagger (CRT). This sub-system was developed by the Laboratory for High Energy Physics (LHEP), Albert Einstein center for fundamental physics, University of Bern. The system utilizes plastic scintillation modules to provide precise time and position information for TPC-traversing particles. Successful matching of TPC tracks and CRT data will allow us to reduce cosmogenic background and better characterize the light collection system and LArTPC data using cosmic muons. In this paper we describe the design and installation of the MicroBooNE CRT system and provide an overview of a series of tests done to verify the proper operation of the system and its components during installation, commissioning, and physics data-taking