Vector competence of British Culicoides species for Bluetongue virus serotype 8

The proportion of infected vectors which are able to transmit an arbovirus to a susceptible host has a significant impact on the epidemic potential of such a virus. Assessing vector competence is therefore crucial to evaluate accurately the risk posed by such a disease to any non-endemic region. The vector competence of various Culicoides species in Scotland for bluetongue virus serotype 8 (BTV-8) was assessed by a pad-feeding technique, and a high-throughput virus extraction and isolation procedure. This was coupled with a multiplex polymerase chain reaction (PCR) to identify members of the Culicoides Obsoletus complex to species level. These results are compared with vector competence results of further Culicoides Obsoletus in South-East England assessed by the same method. A very low level of competence for this strain was detected in all Culicoides species tested, similar to that described for this strain in C. imicola originating from both Corsica and the Onderstepoort Veterinary Institute in South Africa. The implications of this are discussed in relation to future studies and also with regard to wider aspects of orbivirus transmission in the European Union. (Texte intégral

Letting the Future In: a therapeutic intervention for children affected by sexual abuse and their carers:An evaluation of impact and implementation

Letting the Future In is a structured guide to therapeutic intervention with children affected by sexual abuse. The guide was developed by the NSPCC and has been implemented by 20 NSPCC teams across England, Wales and Northern Ireland since 2011. It is available to children aged between four and 17 who have made a disclosure and experienced sexual abuse, live with a safe carer with no planned moves and have no diagnosed learning disability. Letting the Future In is grounded in an understanding of trauma, attachment and resilience. It is largely psychodynamic in nature and emphasises the therapeutic attunement of the practitioner to the child’s emotional responses to abuse, which typically include betrayal, powerlessness, shame and traumatic sexualisation. It sees the therapeutic relationship between child and practitioner as ‘core’ and employs creative therapies with work on the awareness and management of feelings. It also draws on other methods including counselling and socio-educative approaches. Children receive up to four therapeutic assessment sessions followed by up to 20 intervention sessions, extended up to 30 if necessary. At the same time, their safe carer is offered up to eight sessions to help them process the impact of discovering that their child was sexually abused, and to support the child in their recovery.There are few rigorous evaluations of therapeutic interventions for these children. Recognising that Letting the Future In is new and untested, the NSPCC commissioned a process and impact evaluation from the universities of Bristol and Durham

Can insecticide-treated netting provide protection for Equids from Culicoides biting midges in the United Kingdom?

BACKGROUND: Biting midges of the genus Culicoides Latreille, 1809 (Diptera: Ceratopogonidae) cause a significant biting nuisance to equines and are responsible for the biological transmission of African horse sickness virus (AHSV). While currently restricted in distribution to sub-Saharan Africa, AHSV has a history of emergence into southern Europe and causes one of the most lethal diseases of horses and other species of Equidae. In the event of an outbreak of AHSV, the use of insecticide treated nets (ITNs) to screen equine accomodation is recommended by competent authorities including the Office International des Épizooties (OIE) in order to reduce vector-host contact. METHODS: Seven commercially avaliable pyrethroid insecticides and three repellent compounds, all of which are licensed for amateur use, were assessed in modified World Health Organization (WHO) cone bioassay trials in the laboratory using a colony line of Culicoides nubeculosus (Meigen), 1830. Two field trials were subsequently conducted to test the efficiency of treated net screens in preventing entry of Culicoides. RESULTS: A formulation of cypermethrin (0.15 % w/w) and pyrethrins (0.2 % w/w) (Tri-Tec 14®, LS Sales (Farnham) Ltd, Bloxham, UK) applied to black polyvinyl-coated polyester insect screen (1.6 mm aperture; 1.6 mm thickness) inflicted 100 % mortality on batches of C. nubeculosus following a three minute exposure in the WHO cone bioassays at 1, 7 and 14 days post-treatment. Tri-Tec 14® outperformed all other treatments tested and was subsequently selected for use in field trials. The first trial demonstrated that treated screens placed around an ultraviolet light-suction trap entirely prevented Culicoides being collected, despite their collection in identical traps with untreated screening or no screening. The second field trial examined entry of Culicoides into stables containing horses and found that while the insecticide treated screens reduced entry substantially, there was still a small risk of exposure to biting. CONCLUSIONS: Screened stables can be utilised as part of an integrated control program in the event of an AHSV outbreak in order to reduce vector-host contact and may also be applicable to protection of horses from Culicoides during transport.The work of LEH and GLI was supported by funding from the Horserace Betting Levy Board (HBLB) (Vet/PRJ/766); TR was supported by funding from the University of Surrey; and JW was supported by the Alborada Trust and by the European Union FP7 project ANTIGONE (contract number 278976). RN is supported through a combined contribution to the Animal Health Trust’s Equine Infectious Disease Service from the HBLB, the Racehorse Owners’ Association (ROA) and the Thoroughbred Breeders’ Association (TBA). The Pirbright Institute receives grant aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom.This is the final version of the article. It was first available from BioMed Central via http://dx.doi.org/10.1186/s13071-015-1182-

Blood-feeding, susceptibility to infection with Schmallenberg virus and phylogenetics of Culicoides (Diptera: Ceratopogonidae) from the United Kingdom

BACKGROUND: Culicoides biting midges (Diptera: Ceratopogonidae) are responsible for the biological transmission of internationally important arboviruses of livestock. In 2011, a novel Orthobunyavirus was discovered in northern Europe causing congenital malformations and abortions in ruminants. From field studies, Culicoides were implicated in the transmission of this virus which was subsequently named Schmallenberg virus (SBV), but to date no assessment of susceptibility to infection of field populations under standardised laboratory conditions has been carried out. We assessed the influence of membrane type (chick skin, collagen, Parafilm M®) when offered in conjunction with an artificial blood-feeding system (Hemotek, UK) on field-collected Culicoides blood-feeding rates. Susceptibility to infection with SBV following blood-feeding on an SBV-blood suspension provided via either (i) the Hemotek system or via (ii) a saturated cotton wool pledglet was then compared. Schmallenberg virus susceptibility was defined by RT-qPCR of RNA extractions of head homogenates and related to Culicoides species and haplotype identifications based on the DNA barcode region of the mitochondrial cytochrome c oxidase 1 (cox1) gene. RESULTS: Culicoides blood-feeding rates were low across all membrane types tested (7.5% chick skin, 0.0% for collagen, 4.4% Parafilm M®, with 6029 female Culicoides being offered a blood meal in total). Susceptibility to infection with SBV through membrane blood-feeding (8 of 109 individuals tested) and pledglet blood-feeding (1 of 94 individuals tested) was demonstrated for the Obsoletus complex, with both C. obsoletus (Meigen) and C. scoticus Downes & Kettle susceptible to infection with SBV through oral feeding. Potential evidence of cryptic species within UK populations was found for the Obsoletus complex in phylogenetic analyses of cox1 DNA barcodes of 74 individuals assessed from a single field-site. CONCLUSIONS: Methods described in this study provide the means to blood-feed Palaearctic Culicoides for vector competence studies and colonisation attempts. Susceptibility to SBV infection was 7.3% for membrane-fed members of the subgenus Avaritia and 1.1% for pledglet-fed. Both C. obsoletus and C. scoticus were confirmed as being susceptible to infection with SBV, with potential evidence of cryptic species within UK Obsoletus complex specimens, however the implications of cryptic diversity in the Obsoletus complex on arbovirus transmission remains unknow

A comparison of commercial light-emitting diode baited suction traps for surveillance of Culicoides in northern Europe

BACKGROUND: The response of Culicoides biting midges (Diptera: Ceratopogonidae) to artificial light sources has led to the use of light-suction traps in surveillance programmes. Recent integration of light emitting diodes (LED) in traps improves flexibility in trapping through reduced power requirements and also allows the wavelength of light used for trapping to be customized. This study investigates the responses of Culicoides to LED light-suction traps emitting different wavelengths of light to make recommendations for use in surveillance. METHODS: The abundance and diversity of Culicoides collected using commercially available traps fitted with Light Emitting Diode (LED) platforms emitting ultraviolet (UV) (390 nm wavelength), blue (430 nm), green (570 nm), yellow (590 nm), red (660 nm) or white light (425 nm – 750 nm with peaks at 450 nm and 580 nm) were compared. A Centre for Disease Control (CDC) UV light-suction trap was also included within the experimental design which was fitted with a 4 watt UV tube (320-420 nm). Generalised linear models with negative binomial error structure and log-link function were used to compare trap abundance according to LED colour, meteorological conditions and seasonality. RESULTS: The experiment was conducted over 49 nights with 42,766 Culicoides caught in 329 collections. Culicoides obsoletus Meigen and Culicoides scoticus Downes and Kettle responded indiscriminately to all wavelengths of LED used with the exception of red which was significantly less attractive. In contrast, Culicoides dewulfi Goetghebuer and Culicoides pulicaris Linnaeus were found in significantly greater numbers in the green LED trap than in the UV LED trap. The LED traps collected significantly fewer Culicoides than the standard CDC UV light-suction trap. CONCLUSIONS: Catches of Culicoides were reduced in LED traps when compared to the standard CDC UV trap, however, their reduced power requirement and small size fulfils a requirement for trapping in logistically challenging areas or where many traps are deployed at a single site. Future work should combine light wavelengths to improve trapping sensitivity and potentially enable direct comparisons with collections from hosts, although this may ultimately require different forms of baits to be developed. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-0846-x) contains supplementary material, which is available to authorized users

Accurate free and forced rotational motions of rigid Venus

% context :The precise and accurate modelling of a terrestrial planet like Venus is an exciting and challenging topic, all the more interesting since it can be compared with that of the Earth for which such a modelling has already been achieved at the milliarcsecond level % aims: We want to complete a previous study (Cottereau and Souchay, 2009), by determining at the milliarcsecond level the polhody, i.e. the torque-free motion of the axis of angular momentum of a rigid Venus in a body-fixed frame, as well as the nutation of its third axis of figure in space, which is fundamental from an observational point of view. results :In a first part we have computed the polhody, i.e. the respective free rotational motion of the axis of angular momentum of Venus with respect to a body-fixed frame. We have shown that this motion is highly elliptical, with a very long period of 525 cy to be compared with 430 d for the Earth. This is due to the very small dynamical flattening of Venus in comparison with our planet. In a second part we have computed precisely the Oppolzer terms which allow to represent the motion in space of the third Venus figure axis with respect to Venus angular momentum axis, under the influence of the solar gravitational torque. We have determined the corresponding tables of coefficients of nutation of the third figure axis both in longitude and in obliquity due to the Sun, which are of the same order of amplitude as for the Earth. We have shown that the coefficients of nutation for the third figure axis are significantly different from those of the angular momentum axis on the contrary of the Earth. Our analytical results have been validated by a numerical integration which revealed the indirect planetary effects.Comment: 14 pages, 11 figures, accepted for publication in section 11. Celestial mechanics and astrometry of Astronomy and Astrophysics (27/02/2010

The biting midge Culicoides sonorensis (Diptera Ceratopogonidae) is capable of developing late stage infections of Leishmania enriettii

BACKGROUND: Despite their importance in animal and human health, the epidemiology of species of the Leishmania enriettii complex remains poorly understood, including the identity of their biological vectors. Biting midges of the genus Forcipomyia (Lasiohelea) have been implicated in the transmission of a member of the L. enriettii complex in Australia, but the far larger and more widespread genus Culicoides has not been investigated for the potential to include vectors to date. METHODOLOGY/PRINCIPAL FINDINGS: Females from colonies of the midges Culicoides nubeculosus Meigen and C. sonorensis Wirth & Jones and the sand fly Lutzomyia longipalpis Lutz & Nevia (Diptera: Psychodidae) were experimentally infected with two different species of Leishmania, originating from Australia (Leishmania sp. AM-2004) and Brazil (Leishmania enriettii). In addition, the infectivity of L. enriettii infections generated in guinea pigs and golden hamsters for Lu. longipalpis and C. sonorensis was tested by xenodiagnosis. Development of L. enriettii in Lu. longipalpis was relatively poor compared to other Leishmania species in this permissive vector. Culicoides nubeculosus was not susceptible to infection by parasites from the L. enriettii complex. In contrast, C. sonorensis developed late stage infections with colonization of the thoracic midgut and the stomodeal valve. In hamsters, experimental infection with L. enriettii led only to mild symptoms, while in guinea pigs L. enriettii grew aggressively, producing large, ulcerated, tumour-like lesions. A high proportion of C. sonorensis (up to 80%) feeding on the ears and nose of these guinea pigs became infected. CONCLUSIONS/SIGNIFICANCE: We demonstrate that L. enriettii can develop late stage infections in the biting midge Culicoides sonorensis. This midge was found to be susceptible to L. enriettii to a similar degree as Lutzomyia longipalpis, the vector of Leishmania infantum in South America. Our results support the hypothesis that some biting midges could be natural vectors of the L. enriettii complex because of their vector competence, although not Culicoides sonorensis itself, which is not sympatric, and midges should be assessed in the field while searching for vectors of related Leishmania species including L. martiniquensis and "L. siamensis"

Friend or Foe? Inter-agency Cooperation, Organizational Reputation, and Turf

AcceptedArticleThis article aims to explain two contrasting cases of bureaucratic cooperation: the cooperation practices of two similar European agencies – Europol and Frontex – with corresponding national-level structures. To understand why cooperation has proceeded smoothly in one case (border management), while triggering strong turf-protective tendencies in the other (law enforcement), the article develops a theoretical approach to cooperation that is both ‘turf’ and reputation sensitive. Drawing on a variety of documents and interview material, the article demonstrates that the divergent outcomes are shaped to a large extent by the different reputational impact of cooperation for the national authorities concerned. In one case, cooperation depletes important reputational resources of national authorities, threatening their ‘reputational uniqueness’ and triggering turf-protective tendencies. In the other, vertical and horizontal cooperation efforts bring important gains to national authorities' ability to discharge their tasks successfully and, thus, to their reputation-building efforts. Crucially however, they do so without threatening their ‘reputational uniqueness’