Ciliary Hedgehog signaling patterns the digestive system to generate mechanical forces driving elongation

The mechanisms underlying tubular organ elongation remain poorly understood. Here, the authors show that primary cilia interpret Hedgehog signals to pattern the developing gut and that smooth muscle in the gut wall generates mechanical forces that direct longitudinal growth. How tubular organs elongate is poorly understood. We found that attenuated ciliary Hedgehog signaling in the gut wall impaired patterning of the circumferential smooth muscle and inhibited proliferation and elongation of developing intestine and esophagus. Similarly, ablation of gut-wall smooth muscle cells reduced lengthening. Disruption of ciliary Hedgehog signaling or removal of smooth muscle reduced residual stress within the gut wall and decreased activity of the mechanotransductive effector YAP. Removing YAP in the mesenchyme also reduced proliferation and elongation, but without affecting smooth muscle formation, suggesting that YAP interprets the smooth muscle-generated force to promote longitudinal growth. Additionally, we developed an intestinal culture system that recapitulates the requirements for cilia and mechanical forces in elongation. Pharmacologically activating YAP in this system restored elongation of cilia-deficient intestines. Thus, our results reveal that ciliary Hedgehog signaling patterns the circumferential smooth muscle to generate radial mechanical forces that activate YAP and elongate the gut.Peer reviewe

Negative density-distribution relationship in butterflies

Peer reviewe

A machine learning system for automated whole-brain seizure detection

Epilepsy is a chronic neurological condition that affects approximately 70 million people worldwide. Characterised by sudden bursts of excess electricity in the brain, manifesting as seizures, epilepsy is still not well understood when compared with other neurological disorders. Seizures often happen unexpectedly and attempting to predict them has been a research topic for the last 30 years. Electroencephalograms have been integral to these studies, as the recordings that they produce can capture the brain’s electrical signals. The diagnosis of epilepsy is usually made by a neurologist, but can be difficult to make in the early stages. Supporting para-clinical evidence obtained from magnetic resonance imaging and electroencephalography may enable clinicians to make a diagnosis of epilepsy and instigate treatment earlier. However, electroencephalogram capture and interpretation is time consuming and can be expensive due to the need for trained specialists to perform the interpretation. Automated detection of correlates of seizure activity generalised across different regions of the brain and across multiple subjects may be a solution. This paper explores this idea further and presents a supervised machine learning approach that classifies seizure and non-seizure records using an open dataset containing 342 records (171 seizures and 171 non-seizures). Our approach posits a new method for generalising seizure detection across different subjects without prior knowledge about the focal point of seizures. Our results show an improvement on existing studies with 88% for sensitivity, 88% for specificity and 93% for the area under the curve, with a 12% global error, using the k-NN classifier

The use of airborne laser scanning to develop a pixel-based stratification for a verified carbon offset project

Background The voluntary carbon market is a new and growing market that is increasingly important to consider in managing forestland. Monitoring, reporting, and verifying carbon stocks and fluxes at a project level is the single largest direct cost of a forest carbon offset project. There are now many methods for estimating forest stocks with high accuracy that use both Airborne Laser Scanning (ALS) and high-resolution optical remote sensing data. However, many of these methods are not appropriate for use under existing carbon offset standards and most have not been field tested. Results This paper presents a pixel-based forest stratification method that uses both ALS and optical remote sensing data to optimally partition the variability across an ~10,000 ha forest ownership in Mendocino County, CA, USA. This new stratification approach improved the accuracy of the forest inventory, reduced the cost of field-based inventory, and provides a powerful tool for future management planning. This approach also details a method of determining the optimum pixel size to best partition a forest. Conclusions The use of ALS and optical remote sensing data can help reduce the cost of field inventory and can help to locate areas that need the most intensive inventory effort. This pixel-based stratification method may provide a cost-effective approach to reducing inventory costs over larger areas when the remote sensing data acquisition costs can be kept low on a per acre basis

Tree Cover Mapping Based on Sentinel-2 Images Demonstrate High Thematic Accuracy in Europe

The spatial and temporal distribution of trees has a large impact on human health and the environment through contributions to important climate mechanisms as well as commercial, recreational and social activities in society. A range of tree mapping methodologies has been presented in the literature, but tree cover estimates still differ widely between the individual datasets, and comparisons of the thematic accuracy of the resulting tree maps are rather scarce. The Copernicus Sentinel-2 satellites, which were launched in 2015 and 2017, have a combination of high spatial and temporal resolution. Given that this is a new satellite, a substantial amount of research on development of tree mapping algorithms as well as accuracy assessment of said algorithms have to be done in the years to come. To contribute to this process, a tree map produced through unsupervised classification was created for six Sentinel-2 tiles. The agreement between the tree map and the corresponding national forest inventory, as a function of the band combination chosen, was analysed and the thematic accuracy was assessed for two out of the six tiles. The results show that the highest agreement between the present tree map and the national forest inventory was found for bands 2, 3, 6 and 12. The present tree map has a relative difference in tree cover between 8% and 79% compared to previous estimates, but results are characterised by large scatter. Lastly, it is shown that the overall thematic accuracy of the present map is up to 90%, with the user’s accuracy ranging from 34.85 % to 92.10 %, and the producer’s accuracy ranging from 23.80 % to 97.60 % for the various thematic classes. This demonstrates that tree maps with high thematic accuracy can be produced from Sentinel-2. In the future the thematic accuracy can be increased even more through the use of temporal averaging in the mapping procedure, which will enable an accurate estimate of the European tree cover