Whole-cell redox biocatalysis driven by photosynthesis – an integrated bioprocess design for phototrophic biocatalysts

Much success was already achieved for the development of efficient oxyfunctionalization bioprocesses by the application of oxygenases in heterotrophic whole-cell host systems. However, several restrictions such as the technically limited O2 supply and carbohydrate-based electron supply still limit their implementation on an industrial scale concerning production rates and costs. The use of phototrophic organisms as whole-cell biocatalysts for oxygenase-based biotransformations provides an alternative and promising technology for the eco-efficient production of oxyfunctionalized value-added chemicals. While numerous cyanobacterial or microalgal bioprocesses were already developed for CO2-derived fermentations, biotransformation processes relying on the generation of activated reduction equivalents as well as O2-derived from photosynthetic water oxidation are rare. In this context, research mainly focuses on the demonstration of engineered catalysts with emphasis on the production of hydrogen. Yet, an integrated bioprocess design for the application of phototrophic organisms in redox biotransformations beyond the proof-of-concept catalyst development is lacking. This thesis aims at the integrated application of biotechnological methods and strategies for the development of eco-efficient photosynthesis-driven oxyfunctionalization processes. The main research question combines the conceptual evaluation of photosynthetic electron and O2 supply with the technical applicability of cyanobacteria as phototrophic host organisms in a hydrocarbon oxyfunctionalization bioprocess. Using a guide of integrated bioprocess design, biocatalyst, reaction, and process engineering tools are applied for the establishment of new, photosynthesis-driven bioprocesses

Partial Degree Formulae for Plane Offset Curves

In this paper we present several formulae for computing the partial degrees of the defining polynomial of the offset curve to an irreducible affine plane curve given implicitly, and we see how these formulae particularize to the case of rational curves. In addition, we present a formula for computing the degree w.r.t the distance variable.Comment: 24 pages, no figure

Zur Berechnung der Hauptkrümmungen und Hauptkrümmungsrichtungen bei empirisch vorgegebenen Flächenstücken

Bei der Untersuchung der Gestalt von Teilen des menschlichen Körpers können die betrachteten Körperteile über ein projiziertes Raster mit Hilfe der Nahbereichsphotogrammetrie optisch vermessen werden. Von den dann durch Punkte Pi empirisch festgelegten Flächenstücken [Phi]e interessieren z.B. bei Anwendungen in der kosmetischen Industrie in den Punkten Pi die extremalen Flächenkrümmungen sowie die Richtungen, in denen diese extremalen Flächenkrümmungen in den Punkten Pi des Flächenstückes [Phi]e auftreten. In den Sprachgebrauch der Differentialgeometrie übertragen, bedeutet dies das Aufsuchen der Hauptkrümmungen und des Richtungsfeldes der Krümmungslinien in den Punkten Pi des empirisch vorgegebenen Flächenstückes [Phi]e. Obwohl im Rahmen der Untersuchungen zur zeichnerischen Darstellung von Flächen (Computer-Design) und im Rahmen der Spline-Approximation von Flächen eine umfangreiche Literatur vorliegt, scheint die obengenannte Problemstellung noch nicht diskutiert worden zu sein. In der vorliegenden Untersuchung soll daher eine Methode zur numerischen Berechnung der Hauptkrümmungen und des Richtungsfeldes der Krümmungslinien in den Punkten Pi eines empirisch vorgegebenen Flächenstückes [Phi]e entwickelt werden. Methodisch werden dabei differentialgeometrische Betrachtungen mit Verfahren aus der Ausgleichsrechnung verknüpft werden. Überlegungen aus der Statistik liefern dann eine Fehlerabschätzung der gefundenen Resultate. Anwendungen auf bekannte Flächenstücke zeigen abschließend die Wirksamkeit der entwickelten Methoden auf

Polynomial cubic splines with tension properties

In this paper we present a new class of spline functions with tension properties. These splines are composed by polynomial cubic pieces and therefore are conformal to the standard, NURBS based CAD/CAM systems

A tension approach to controlling the shape of cubic spline surfaces on FVS triangulations

We propose a parametric tensioned version of the FVS macro-element to control the shape of the composite surface and remove artificial oscillations, bumps and other undesired behaviour. In particular, this approach is applied to C1 cubic spline surfaces over a four-directional mesh produced by two-stage scattered data fitting methods

Total Degree Formula for the Generic Offset to a Parametric Surface

We provide a resultant-based formula for the total degree w.r.t. the spatial variables of the generic offset to a parametric surface. The parametrization of the surface is not assumed to be proper.Comment: Preprint of an article to be published at the International Journal of Algebra and Computation, World Scientific Publishing, DOI:10.1142/S021819671100680

Geometric characteristics of conics in Bézier form

In this paper, we address the calculation of geometric characteristics of conic sections (axes, asymptotes, centres, eccentricity, foci) given in Bézier form in terms of their control polygons and weights, making use of real and complex projective and affine geometry and avoiding the use of coordinates

Perception, understanding, and action: attitudes of climate change in the Hungarian population

This study is based on a non-representative, national level survey sample whose main purpose is to interpret the general population’s understanding of climate change. The study also provides an examination of correlations between climate change concerns and the taking of individual action as well as the relationship between pro-environmental thinking and climate change scepticism. Our results show a moderate correlation between the general population’s concerns and the professional views on the subject, known in the literature as the New Environmental Paradigm scale and Scepticism scale, but a significantly weaker correlation when it comes to taking action against climate change. Factors relating to the respondents, such as residence settlement type, education level, gender, age, personal and social values, or casual attributions in relation to climate change heavily influence this weaker correlation. Most respondents assessed climate change as a current (urgent), but geographically remote phenomenon. This is a clear indication of problems associated with cognitive conceptualization and the localization of climate change in communication. The target audience must be taken into account when designing climate change communications because interpretations of climate change can vary widely and cover a broad range attitudes ranging from concern about to issue all the way to climate change scepticism. This also applies to views concerning responsibility for climate change with some believing it is a political responsibility and others believing it is a scientific responsibility