Assessing intestinal permeability in Crohn's disease patients using orally administered ⁵²Cr-EDTA

BACKGROUND : Intestinal permeability can be assessed by monitoring renal excretion of orally administered radioactively 51Cr-labeled ethylenediaminetetraacetic acid (51Cr-EDTA). Although considered safe, patient participation in using radio-labeled tracers is low. Here, we used orally administered 52Cr-EDTA as non-radioactive alternative to assess intestinal permeability in CD and analyzed the association with disease activity, disease location and gut microbial dysbiosis. MATERIALS AND METHODS : 60 CD patients with low (n = 25) and increased (n = 35) fecal calprotectin levels (cut-off: 100 µg/g feces) ingested 20 mL 52Cr-EDTA (20 mmol/L) solution whereafter 24-h urine was collected. Urinary 52Cr-EDTA concentrations were quantified using Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Fecal Enterobacteriaceae and Faecalibacterium prausnitzii were quantified using FISH. Correlations between urinary 52Cr-EDTA excretion and other parameters were established using nonparametric Spearman’s correlation coefficients (ρ). RESULTS : CD patients with increased fecal calprotectin levels (> 100 µg/g) demonstrated an elevated urinary 52Cr-EDTA/creatinine ratio (772 vs. 636 μmol/mol, P = 0.132). Patients with primarily colonic disease showed the highest 52Cr-EDTA excretion. Importantly, a positive correlation was observed for the urinary 52Cr-EDTA/creatinine ratio and fecal calprotectin levels (ρ = 0.325, P < 0.05). Finally, urinary 52Cr-EDTA/creatinine ratio negatively correlated with the relative abundance of Faecalibacterium prausnitzii (ρ = -0.221, P = 0.092), while positively correlating with Enterobacteriaceae (ρ = 0.202, P = 0.126). CONCLUSIONS : Orally administered and renal excreted 52Cr-EDTA may be used to assess intestinal permeability in CD and correlates with fecal calprotectin levels and bacterial species relevant to CD. This test may improve non-invasive detection of disease exacerbations and help monitor disease activity

Shallow Dark Matter Cusps in Galaxy Clusters

We study the evolution of the stellar and dark matter components in a galaxy cluster of $10^{15} \, \rm{M_{\odot}}$ from $z=3$ to the present epoch using the high-resolution collisionless simulations of Ruszkowski & Springel (2009). At $z=3$ the dominant progenitor halos were populated with spherical model galaxies with and without accounting for adiabatic contraction. We apply a weighting scheme which allows us to change the relative amount of dark and stellar material assigned to each simulation particle in order to produce luminous properties which agree better with abundance matching arguments and observed bulge sizes at $z=3$. This permits the study of the effect of initial compactness on the evolution of the mass-size relation. We find that for more compact initial stellar distributions the size of the final Brightest Cluster Galaxy grows with mass according to $r\propto M^{2}$, whereas for more extended initial distributions, $r\propto M$. Our results show that collisionless mergers in a cosmological context can reduce the strength of inner dark matter cusps with changes in logarithmic slope of 0.3 to 0.5 at fixed radius. Shallow cusps such as those found recently in several strong lensing clusters thus do not necessarily conflict with CDM, but may rather reflect on the initial structure of the progenitor galaxies, which was shaped at high redshift by their formation process.Comment: 8 pages, 4 figures, submitted to MNRA

The properties of Brightest Cluster Galaxies in the SDSS DR6 adaptive matched filter cluster catalogue

We study the properties of Brightest Cluster Galaxies (BCGs) drawn from a catalogue of more than 69000 clusters in the SDSS DR6 based on the adaptive matched filter technique (AMF, Szabo et al., 2010). Our sample consists of more than 14300 galaxies in the redshift range 0.1-0.3. We test the catalog by showing that it includes well-known BCGs which lie in the SDSS footprint. We characterize the BCGs in terms of r-band luminosities and optical colours as well as their trends with redshift. In particular, we define and study the fraction of blue BCGs, namely those that are likely to be missed by either colour-based cluster surveys and catalogues. Richer clusters tend to have brighter BCGs, however less dominant than in poorer systems. 4-9% of our BCGs are at least 0.3 mag bluer in the g-r colour than the red-sequence at their given redshift. Such a fraction decreases to 1-6% for clusters above a richness of 50, where 3% of the BCGs are 0.5 mag below the red-sequence. A preliminary morphological study suggests that the increase in the blue fraction at lower richnesses may have a non-negligible contribution from spiral galaxies. We show that a colour selection based on the g-r red-sequence or on a cut at colour u-r >2.2 can lead to missing the majority of such blue BCGs. We also extend the colour analysis to the UV range by cross-matching our catalogue with publicly available data from Galex GR4 and GR5. We show a clear correlation between offset from the optical red-sequence and the amount of UV-excess. Finally, we cross-matched our catalogue with the ACCEPT cluster sample (Cavagnolo et al., 2009), and find that blue BCGs tend to be in clusters with low entropy and short cooling times. That is, the blue light is presumably due to recent star formation associated to gas feeding by cooling flows. (abridged)Comment: 15 pages, 15 figures, submitted to MNRA

Lead exposure in adult males in urban Transvaal Province, South Africa during the apartheid era

Human exposure to lead is a substantial public health hazard worldwide and is particularly problematic in the Republic of South Africa given the country’s late cessation of leaded petrol. Lead exposure is associated with a number of serious health issues and diseases including developmental and cognitive deficiency, hypertension and heart disease. Understanding the distribution of lifetime lead burden within a given population is critical for reducing exposure rates. Femoral bone from 101 deceased adult males living in urban Transvaal Province (now Gauteng Province), South Africa between 1960 and 1998 were analyzed for lead concentration by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Of the 72 black and 29 white individuals sampled, chronic lead exposure was apparent in nearly all individuals. White males showed significantly higher median bone lead concentration (ME = 10.04 µg·g−1), than black males (ME = 3.80 µg·g−1) despite higher socioeconomic status. Bone lead concentration covaries significantly, though weakly, with individual age. There was no significant temporal trend in bone lead concentration. These results indicate that long-term low to moderate lead exposure is the historical norm among South African males. Unexpectedly, this research indicates that white males in the sample population were more highly exposed to lead

Halo Occupation Distribution Modeling of Green Valley Galaxies

We present a clustering analysis of near ultraviolet (NUV) - optical color selected luminosity bin samples of green valley galaxies. These galaxy samples are constructed by matching the Sloan Digital Sky Survey Data Release 7 with the latest Galaxy Evolution Explorer source catalog which provides NUV photometry. We present cross-correlation function measurements and determine the halo occupation distribution of these transitional galaxies using a new multiple tracer analysis technique. We extend the halo-occupation formalism to model the cross-correlation function between a galaxy sample of interest and multiple tracer populations simultaneously. This method can be applied to commonly used luminosity threshold samples as well as to color and luminosity bin selected galaxy samples, and improves the accuracy of clustering analyses for sparse galaxy populations. We confirm the previously observed trend that red galaxies reside in more massive halos and are more likely to be satellite galaxies than average galaxies of similar luminosity. While the change in central galaxy host mass as a function of color is only weakly constrained, the satellite fraction and characteristic halo masses of green satellite galaxies are found to be intermediate between those of blue and red satellite galaxies.Comment: matches MNRAS accepted version; minor revisions, results unchange

Evolution of Stellar Collision Products in Globular Clusters - II. Off-axis Collision

We continue our exploration of collisionally merged stars in the blue straggler region of the color-magnitude diagram. We report the results of new SPH calculations of parabolic collisions between two main-sequence stars, with the initial structure and composition profiles of the parent stars having been determined from stellar evolution calculations. Parallelization of the SPH code has permitted much higher numerical resolution of the hydrodynamics. We also present evolutionary tracks for the resulting collision products, which emerge as rapidly rotating blue stragglers. The rotating collision products are brighter, bluer and remain on the main sequence longer than their non-rotating counterparts. In addition, they retain their rapid rotation rates throughout their main sequence lifetime. Rotationally-induced mixing strongly affects the evolution of the collision products, although it is not sufficient to mix the entire star. We discuss the implications of these results for studies of blue straggler populations in clusters. This work shows that off-axis collision products cannot become blue stragglers unless they lose a large fraction of their initial angular momentum. The mechanism for this loss is not apparent, although some possibilities are discussed.Comment: 25 pages incl. 9 figures (one in colour). Submitted to Ap

Randomised Controlled Trial of Unsolicited Occupational Therapy in Community-Dwelling Elderly People: The LOTIS Trial

OBJECTIVE: The objective of this trial, the Leiden 85-Plus Occupational Therapy Intervention Study (LOTIS), was to assess whether unsolicited occupational therapy, as compared to no therapy, can decelerate the increase in disability in high-risk elderly people. DESIGN: This was a randomised controlled trial with 2-y follow-up. SETTING: The study took place in the municipality of Leiden in the Netherlands. PARTICIPANTS: The participants were 402 community-dwelling 85-y-old people, with a Mini-Mental State Examination score of >18 points at baseline. INTERVENTIONS: Participants in the intervention group were visited by an occupational therapist who provided training and education about assistive devices that were already present and who gave recommendations and information about procedures, possibilities, and costs of assistive devices and community-based services. Control participants were not visited by an occupational therapist. OUTCOME MEASURES: The primary outcome measure was the score achieved on the Groningen Activity Restriction Scale. Secondary outcome measures included self-evaluations of well-being and feelings of loneliness. RESULTS: The participants were evenly divided between the two groups: 202 participants were allocated to the intervention group and 200 participants to the control group. Of the 202 participants randomised to occupational therapy, 55 participants declined the proposed intervention. An occupational therapist indicated that of the remaining 147 participants, 66 (45%) needed an occupational therapy intervention. A total of 44 new assistive devices and five community-based services were implemented. During follow-up there was a progressive increase in disability in the intervention group (mean annual increase, 2.0 points; SE 0.2; p < 0.001) and control group (mean annual increase, 2.1 points; SE 0.2; p < 0.001). The increase in disability was not significantly different between study groups (0.08 points; 95% CI, −1.1–1.2; p = 0.75). There was also no difference between study groups for any of the secondary outcome measures. CONCLUSION: Unsolicited occupational therapy in high-risk elderly participants does not decelerate the increase in disability over time