Determining the velocity distribution in the boundary layer of an airfoil fitted with a rotary cylinder

In the closer investigation of the results obtained from a wing model with a rotary cylinder mounted in its leading edge (NACA TM's 307 and 354), the velocity distribution in the vicinity of the surface of the model was determined by a hot-wire anemometer. The results confirmed the belief that the rotary cylinder had considerable effect on the air flow, but demonstrated the fact that the direct influence of the cylinder is confined to a very thin layer in immediate proximity to the surface

Comparison of flow and dispersion properties of free and wall turbulent jets for source dynamics characterisation

The objective of this paper is to provide an investigation, using large eddy simulations, into the dispersion of aircraft jets in co-flowing take-off conditions. Before carrying out such study, simple turbulent plane free and wall jet simulations are carried out to validate the computational models and to assess the impact of the presence of the solid boundary on the flow and dispersion properties. The current study represents a step towards a better understanding of the source dynamics behind an airplane jet engine during the take-off and landing phases. The information provided from these simulations can be used for future improvements of existing dispersion models

Scientific Evidence and Rationale for the Development of Curcumin and Resveratrol as Nutraceutricals for Joint Health

Interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α) are key cytokines that drive the production of inflammatory mediators and matrix-degrading enzymes in osteoarthritis (OA). These proinflammatory cytokines bind to their respective cell surface receptors and activate inflammatory signaling pathways culminating with the activation of nuclear factor κB (NF-κB), a transcription factor that can be triggered by a host of stress-related stimuli including, excessive mechanical stress and ECM degradation products. Once activated, NF-κB regulates the expression of many cytokines, chemokines, adhesion molecules, inflammatory mediators, and several matrix-degrading enzymes. Therefore, proinflammatory cytokines, their cell surface receptors, NF-κB and downstream signaling pathways are therapeutic targets in OA. This paper critically reviews the recent literature and outlines the potential prophylactic properties of plant-derived phytochemicals such as curcumin and resveratrol for targeting NF-κB signaling and inflammation in OA to determine whether these phytochemicals can be used as functional foods

Authenticity and drug resistance in a panel of acute lymphoblastic leukaemia cell lines

Cell lines are important models for drug resistance in acute lymphoblastic leukaemia (ALL), but are often criticised as being unrepresentative of primary disease. There are also doubts regarding the authenticity of many lines. We have characterised a panel of ALL cell lines for growth and drug resistance and compared data with that published for primary patient specimens. In contrast to the convention that cell lines are highly proliferative, those established in our laboratory grow at rates similar to estimates of leukaemic cells in vivo (doubling time 53–442 h). Authenticity was confirmed by genetic fingerprinting, which also demonstrated the potential stability of long-term cultures. In vitro glucocorticoid resistance correlated well with that measured ex vivo, but all lines were significantly more sensitive to vincristine than primary specimens. Sensitivity to methotrexate was inversely correlated to that of glucocorticoids and L-asparaginase, indicating possible reciprocity in resistance mechanisms. A cell line identified as highly methotrexate resistant (IC50 >8000-fold higher than other lines) was derived from a patient receiving escalating doses of the drug, indicating in vivo selection of resistance as a cause of relapse. Many of these lines are suitable as models to study naturally occurring resistance phenotypes in paediatric ALL

Cellular and molecular biology of Neisseria meningitidis colonization and invasive disease

The human species is the only natural host of Neisseria meningitidis, an important cause of bacterial meningitis globally, and, despite its association with devastating diseases, N. meningitidis is a commensal organism found frequently in the respiratory tract of healthy individuals. To date, antibiotic resistance is relatively uncommon in N. meningitidis isolates but, due to the rapid onset of disease in susceptible hosts, the mortality rate remains approx. 10%. Additionally, patients who survive meningococcal disease often endure numerous debilitating sequelae. N. meningitidis strains are classified primarily into serogroups based on the type of polysaccharide capsule expressed. In total, 13 serogroups have been described; however, the majority of disease is caused by strains belonging to one of only five serogroups. Although vaccines have been developed against some of these, a universal meningococcal vaccine remains a challenge due to successful immune evasion strategies of the organism, including mimicry of host structures as well as frequent antigenic variation. N. meningitidis express a range of virulence factors including capsular polysaccharide, lipopolysaccharide and a number of surface-expressed adhesive proteins. Variation of these surface structures is necessary for meningococci to evade killing by host defence mechanisms. Nonetheless, adhesion to host cells and tissues needs to be maintained to enable colonization and ensure bacterial survival in the niche. The aims of the present review are to provide a brief outline of meningococcal carriage, disease and burden to society. With this background, we discuss several bacterial strategies that may enable its survival in the human respiratory tract during colonization and in the blood during infection. We also examine several known meningococcal adhesion mechanisms and conclude with a section on the potential processes that may operate in vivo as meningococci progress from the respiratory niche through the blood to reach the central nervous system

Measurements of the velocity distribution in the boundary layer along a plane surface

Electrical Engineering, Mathematics and Computer Scienc

Prokaryotic argonaute proteins and uses thereof

The invention relates to the field of genetic engineering tools, methods and techniques for nucleic acid, gene or genome editing. Specifically, the invention concerns prokaryotic Argonaute (pAgo) polypeptides having nuclease activity against target DNA when pAgo is complexed with a DNA guide. The invention also provides expression vectors comprising nucleic acids encoding said polypeptides as well as compositions and kits for, and methods of cleaving and editing target nucleic acids in a sequence-specific manner. The polypeptides, nucleic acids, expression vectors, compositions, kits and methods of the invention allow site- specific modifications of genetic material, whether isolated from cells in vitro, or within cells in situ and as such may usefully find application in many fields of biotechnology, including, for example, synthetic biology, gene therapy and agricultural or microbial biotechnology.<br/

NACA Technical Memorandums

In the closer investigation of the results obtained from a wing model with a rotary cylinder mounted in its leading edge (NACA TM's 307 and 354), the velocity distribution in the vicinity of the surface of the model was determined by a hot-wire anemometer. The results confirmed the belief that the rotary cylinder had considerable effect on the air flow, but demonstrated the fact that the direct influence of the cylinder is confined to a very thin layer in immediate proximity to the surface