Candida albicans as an essential "keystone" component within polymicrobial oral biofilm models?

Background: Existing standardized biofilm assays focus on simple mono-species or bacterial-only models. Incorporating Candida albicans into complex biofilm models can offer a more appropriate and relevant polymicrobial biofilm for the development of oral health products. Aims: This study aimed to assess the importance of interkingdom interactions in polymicrobial oral biofilm systems with or without C. albicans, and test how these models respond to oral therapeutic challenges in vitro. Materials and Methods: Polymicrobial biofilms (two models containing 5 and 10 bacterial species, respectively) were created in parallel in the presence and absence of C. albicans and challenged using clinically relevant antimicrobials. The metabolic profiles and biomasses of these complex biofilms were estimated using resazurin dye and crystal violet stain, respectively. Quantitative PCR was utilized to assess compositional changes in microbial load. Additional assays, for measurements of pH and lactate, were included to monitor fluctuations in virulence “biomarkers.” Results: An increased level of metabolic activity and biomass in the presence of C. albicans was observed. Bacterial load was increased by more than a factor of 10 in the presence of C. albicans. Assays showed inclusion of C. albicans impacted the biofilm virulence profiles. C. albicans did not affect the biofilms’ responses to the short-term incubations with different treatments. Conclusions: The interkingdom biofilms described herein are structurally robust and exhibit all the hallmarks of a reproducible model. To our knowledge, these data are the first to test the hypothesis that yeasts may act as potential “keystone” components of oral biofilms. © 2020 by the authors. Licensee MDPI, Basel, Switzerland

A multidisciplinary approach for generating globally consistent data on mesophotic, deep-pelagic, and bathyal biological communities

Approaches to measuring marine biological parameters remain almost as diverse as the researchers who measure them. However, understanding the patterns of diversity in ocean life over different temporal and geographic scales requires consistent data and information on the potential environmental drivers. As a group of marine scientists from different disciplines, we suggest a formalized, consistent framework of 20 biological, chemical, physical, and socioeconomic parameters that we consider the most important for describing environmental and biological variability. We call our proposed framework the General Ocean Survey and Sampling Iterative Protocol (GOSSIP). We hope that this framework will establish a consistent approach to data collection, enabling further collaboration between marine scientists from different disciplines to advance knowledge of the ocean (deep-sea and mesophotic coral ecosystems)

Latent Structures based-Multivariate Statistical Process Control: a paradigm shift

The basic fundamentals of statistical process control (SPC) were proposed by Walter Shewhart for data-starved production environments typical in the 1920s and 1930s. In the 21st century, the traditional scarcity of data has given way to a data-rich environment typical of highly automated and computerized modern processes. These data often exhibit high correlation, rank deficiency, low signal-to-noise ratio, multistage and multiway structures, and missing values. Conventional univariate and multivariate SPC techniques are not suitable in these environments. This article discusses the paradigm shift to which those working in the quality improvement field should pay keen attention. We advocate the use of latent structure based multivariate statistical process control methods as efficient quality improvement tools in these massive data contexts. 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Beyond ‘Blue-Collar Professionalism’ : Continuity and Change in the Professionalization of Uniformed Emergency Services Work

The sociology of professions has so far had limited connections to emergency services occupations. Research on emergency occupations tends to focus on workplace culture and identity, often emphasizing continuity rather than change. Police officers, firefighters and paramedics have their historical roots in manual, technical or ‘semi-professional’ occupations and their working lives still bear many of the hallmarks of blue-collar, uniformed ‘street-level’ work. But uniformed emergency services - like many other occupations – are increasingly undergoing processes of ‘professionalization’. The organizations in which they are employed and the fields in which they work have undergone significant change and disruption, calling into question the core features, cultures and duties of these occupations. This paper argues that sociology of work on emergency services could be helpfully brought into closer contact with the sociology of professions in order to better understand these changes. It suggests four broad empirical and conceptual domains where meaningful connections can be made between these literatures, namely: leadership and authority; organizational goals and objectives; professional identities; and ‘extreme’ work. Emergency services are evolving in complex directions while retaining certain long-standing and entrenched features. Studying emergency occupations as professions also sheds new light on the changing nature of ‘professionalism’ itself

Hepatocellular carcinoma in patients with chronic hepatitis C virus infection without cirrhosis

AIM: To investigate and characterise patients with chronic hepatitis C virus (HCV) infection presenting with hepatocellular carcinoma (HCC) in the absence of cirrhosis