50 research outputs found
Characterising a Si(Li) detector element for the SIXA X-ray spectrometer
The detection efficiency and response function of a Si(Li) detector element
for the SIXA spectrometer have been determined in the 500 eV to 5 keV energy
range using synchrotron radiation emitted at a bending magnet of the electron
storage ring BESSY, which is a primary radiation standard. The agreement
between the measured spectrum and the model calculation is better than 2%.
PACS: 95.55.Ka; 07.85.Nc; 29.40.Wk; 85.30.De
Keywords: Si(Li) detectors, X-ray spectrometers, detector calibration, X-ray
response, spectral lineshapeComment: 11 pages, 11 PostScript figures, uses elsart.sty, submitted to Nucl.
Instrum. Meth.
VUV/EUV ionising radiation and atoms and ions: dual laser plasma investigations
The interaction of ionising radiation with atoms and ions is a key fundamental process. This report concentrates on studies of photoexcitation/photoionisation using laser-produced plasmas as continuum sources and synchronised laser plasma plumes to provide the absorbing atom or ion species. Examples from studies of the interaction of ionising radiation with atoms and ions ranging from few-electron atomic and ionic systems to the many-electron high atomic number actinides are reviewed and illustrate the advantages and limitations of the Dual Laser Plasma technique
Yeast surface display of a noncovalent MHC class II heterodimer complexed with antigenic peptide
Localization of a putative tumor suppressor gene by using homozygous deletions in melanomas.
Efficient Recovery of High-Affinity Antibodies from a Single-Chain Fab Yeast Display Library
CDKN2 gene deletion is not found in chronic lymphoid leukaemias of B- and T-cell origin but is frequent in acute lymphoblastic leukaemia
Libraries against libraries for combinatorial selection of replicating antigen–antibody pairs
Antibodies are among the most highly selective tight-binding ligands for proteins. Because the human genome project has deciphered the proteome, there is an opportunity to use combinatorial antibody libraries to select high-affinity antibodies to every protein encoded by the genome. However, this is a large task because the selection formats used today for combinatorial antibody libraries are geared toward generating antibodies to one antigen at a time. Here, we describe a method that accelerates the identification of antibodies to a multitude of antigens simultaneously by matching combinatorial antibody libraries against eukaryotic antigen libraries so that replication-competent cognate antigen–antibody pairs can be directly selected. Phage and yeast display systems are used because they each link genotype to phenotype and can be replicated individually. When combined with cell sorting, the two libraries can be selected against each other for recovery of cognate antigen–antibody clones in a single experiment