18 research outputs found
Essential Roles of the Tap42-Regulated Protein Phosphatase 2A (PP2A) Family in Wing Imaginal Disc Development of Drosophila melanogaster
Protein ser/thr phosphatase 2A family members (PP2A, PP4, and PP6) are implicated in the control of numerous biological processes, but our understanding of the in vivo function and regulation of these enzymes is limited. In this study, we investigated the role of Tap42, a common regulatory subunit for all three PP2A family members, in the development of Drosophila melanogaster wing imaginal discs. RNAi-mediated silencing of Tap42 using the binary Gal4/UAS system and two disc drivers, pnr- and ap-Gal4, not only decreased survival rates but also hampered the development of wing discs, resulting in a remarkable thorax cleft and defective wings in adults. Silencing of Tap42 also altered multiple signaling pathways (HH, JNK and DPP) and triggered apoptosis in wing imaginal discs. The Tap42RNAi-induced defects were the direct result of loss of regulation of Drosophila PP2A family members (MTS, PP4, and PPV), as enforced expression of wild type Tap42, but not a phosphatase binding defective Tap42 mutant, rescued fly survivorship and defects. The experimental platform described herein identifies crucial roles for Tap42•phosphatase complexes in governing imaginal disc and fly development
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Designing a Climate Program for Puerto Rico Project Center
The climate crisis is a global issue catalyzed by a steady increase in atmospheric levels of greenhouse gases. This project focused on strategies to analyze and mitigate carbon emissions generated by WPI's Global Projects. To achieve this, we estimated the carbon emissions produced on IQP using the Puerto Rico Project Center as a case study, researched and compared carbon offset companies, and spoke to our stakeholders to generate the most effective guide and outreach methods. This work resulted in calculator tools that can be used to estimate IQP emissions, a roadmap of recommended steps towards carbon neutrality and sustainability, and a website to host our information and generate ongoing discussion around the climate crisis and sustainability initiatives
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OTIS Elevator Company Sponsored MQP: Elevator Rope Sway Monitoring System
High-rise buildings are subject to sway due to wind, causing elevator ropes to impact the hoist way, disturbing users, and shortening the elevator’s lifespan. Currently, rope sway is inferred from building sway, but a direct RSMD can more accurately indicate rope sway, improving efficiency and decreasing elevator outages. This study aimed to develop a cost-effective RSMD for the OTIS Elevator Company. The study evaluated various sensor technologies and found that 3D TOF sensors were the most suitable solution due to their accuracy and robustness in measuring rope positions. Sensors were tested and validated using a purpose-built test rig in a hoist way at an OTIS test facility. A system of RSMDs can become the standard monitoring system in the elevator industry, providing real-time information to elevator control systems for improving the safety and efficiency of elevator operations