Identification and characterization of a new branching mutant of Neurospora intermedia from nature

Neurospora is a tropical fungus which is found abundantly growing on burnt sugarcane, discarded corn cobs and other burnt vegetation. It is being used as a model organism for understanding growth and branching in fungi. We have isolated and characterized a naturally occurring branching mutant of Neurospora intermedia which may be useful for understanding growth and branching in fungi

METHOD AND SYSTEM FOR MIGRATING A CODE FROM A FIRST FORMAT TO A SECOND FORMAT

The present disclosure relates to a migration tool to convert a code in a first format to a code in a second format. The source code received can be from a user (108) or from a database (106). The input code is said to be in the first format. An Abstract Syntax tree (AST) of the code in the first format is generated by a system (104) by parsing the code. A modified AST is generated based on the AST of the code in the first format. A code in the second format is generated from the modified AST

Hypoxia-inducible factor (HIF): fuel for cancer progression

Hypoxia is an integral part of the tumor microenvironment, caused primarily due to rapidly multiplying tumor cells and a lack of proper blood supply. Among the major hypoxic pathways, HIF-1 transcription factor activation is one of the widely investigated pathways in the hypoxic tumor microenvironment (TME). HIF-1 is known to activate several adaptive reactions in response to oxygen deficiency in tumor cells. HIF-1 has two subunits, HIF-1β (constitutive) and HIF-1α (inducible). The HIF-1α expression is largely regulated via various cytokines (through PI3K-ACT-mTOR signals), which involves the cascading of several growth factors and oncogenic cascades. These events lead to the loss of cellular tumor suppressant activity through changes in the level of oxygen via oxygen-dependent and oxygenindependent pathways. The significant and crucial role of HIF in cancer progression and its underlying mechanisms have gained much attention lately among the translational researchers in the fields of cancer and biological sciences, which have enabled them to correlate these mechanisms with various other disease modalities. In the present review, we have summarized the key findings related to the role of HIF in the progression of tumors

Advancing sepsis clinical research: harnessing transcriptomics for an omics-based strategy - a comprehensive scoping review

Sepsis continues to be recognized as a significant global health challenge across all ages and is characterized by a complex pathophysiology. In this scoping review, PRISMA-ScR guidelines were adhered to, and a transcriptomic methodology was adopted, with the protocol registered on the Open Science Framework. We hypothesized that gene expression analysis could provide a foundation for establishing a clinical research framework for sepsis. A comprehensive search of the PubMed database was conducted with a particular focus on original research and systematic reviews of transcriptomic sepsis studies published between 2012 and 2022. Both coding and non-coding gene expression studies have been included in this review. An effort was made to enhance the understanding of sepsis at the mRNA gene expression level by applying a systems biology approach through transcriptomic analysis. Seven crucial components related to sepsis research were addressed in this study: endotyping (n = 64), biomarker (n = 409), definition (n = 0), diagnosis (n = 1098), progression (n = 124), severity (n = 451), and benchmark (n = 62). These components were classified into two groups, with one focusing on Biomarkers and Endotypes and the other oriented towards clinical aspects. Our review of the selected studies revealed a compelling association between gene transcripts and clinical sepsis, reinforcing the proposed research framework. Nevertheless, challenges have arisen from the lack of consensus in the sepsis terminology employed in research studies and the absence of a comprehensive definition of sepsis. There is a gap in the alignment between the notion of sepsis as a clinical phenomenon and that of laboratory indicators. It is potentially responsible for the variable number of patients within each category. Ideally, future studies should incorporate a transcriptomic perspective. The integration of transcriptomic data with clinical endpoints holds significant potential for advancing sepsis research, facilitating a consensus-driven approach, and enabling the precision management of sepsis

Role of Ayurvedic Herbs and Shirodhara Procedure in the Management of Academic Stress in Children

Stress is a state of mental strain for a specific person experiencing issues with their social and environmental well-being, which can result in a variety of illnesses. The important phage is when a person is young since this is when their life is going through a lot of change. They are expected to be members of the social elite. Therefore, kids should improve their stress-management skills to live healthy lives after they join the society. Comparing stress to Avasad in Ayurveda (Vatanatamaja vikaras). It's regarded as being among the Manasik Rogas. Ayurveda deals with the mind and body of human beings. Only the conventional system of medicine helps to maintain health in the healthy while also treating the sick. The Ahar vihar, Aushad, and Sanshodhan principles of Ayurveda's therapy are included. Two types of medication internal and external are described in the classical texts of Ayurveda. Many herbal and herbal-mineral concoctions are referred to be "Medhya medicines" in internal medicine. Like Mandukparni Brahmi, Vacha, Shankhpushpi, Giloy, Jyotismati, Ashwagandha, Tagar, etc., the majority of them have been scientifically and therapeutically shown to be effective stress relievers. In terms of external medicine, Panchakarma techniques like Shirodhara are also described. Shirodhara treatment is frequently used to treat psychological disorders. Although Shirodhara's clinical effectiveness has been established, its mode of action is complicated. Shirodhara aids in soothing the anxious mind and relaxing the entire body because the body, mind, and spirit are all interconnected. Shirodhara's therapeutic effectiveness has been established, however, its mode of action is complicated. Since the body, mind, and spirit are all intertwined, Shirodhara helps to quiet a busy mind and rest the entire body

Dexamethasone in children mechanically ventilated for lower respiratory tract infection caused by respiratory syncytial virus:a randomized controlled trial

OBJECTIVE: To determine the efficacy of dexamethasone in the treatment of mechanically ventilated children with respiratory syncytial virus-severe lower respiratory tract infection.DESIGN: International, multicenter, randomized, double-blind, placebo-controlled trial.SETTING: Twelve pediatric intensive care units.SUBJECTS: Children (&lt;2 yrs) mechanically ventilated for respiratory syncytial virus lower respiratory tract infection. Children were prestratified for severity of oxygen abnormalities on admission.INTERVENTION: Intravenous dexamethasone (0.6 mg/kg/day, 48 hrs) or placebo.MEASUREMENTS AND MAIN RESULTS: A superiority approach was used in the subgroup of patients with mild oxygen abnormalities (arterial partial pressure of oxygen/fractional inspired oxygen concentration [PaO(2)/FIO(2)] &gt;200 mm Hg and/or mean arterial pressure ≤10 cm H(2)O) and a noninferiority approach in those with severe oxygen abnormalities (PaO(2)/FIO(2) ≤200 mm Hg and mean arterial pressure &gt;10 cm H(2)O). Primary outcome was the duration of mechanical ventilation. In the subgroup with mild oxygenation abnormalities, 45 of the 89 included patients received dexamethasone and 44 placebo; in the subgroup with severe oxygenation abnormalities, 28 of the 56 included patients received dexamethasone and 28 placebo. Baseline characteristics in both treatment arms were similar for both subgroups. After the third interim analysis, the trial was stopped early for futility taking the slow enrollment into account. At that time, the median duration (interquartile range) of mechanical ventilation was 137 (91-195) hrs in the dexamethasone- and 139 (117-188) hrs in the placebo-treated patients in the subgroup with mild oxygenation abnormalities (p = .6). In the subgroup with severe oxygenation abnormalities, it was 171 (136-212) hrs in the dexamethasone- and 170 (125-201) hrs in the placebo-treated patients (p = .6).CONCLUSION: In this prematurely ended trial in children mechanically ventilated for severe respiratory syncytial virus-lower respiratory tract infection, we found no evidence of a beneficial effect of dexamethasone in children with mild oxygenation abnormalities. Neither was evidence found that dexamethasone may prolong mechanical ventilation in those with severe oxygenation abnormalities.</p

Role of Pathya-Apathya in the Management of Prameha (Type-2 Diabetes Mellitus)

Before starting management of any disease three important things to be kept in mind that is etiology, well planning, and Pathya-Apathya. Pathya–Apathya&nbsp;plays important role in the management of various disorders. Prameha is one of such disorder in which Pathya-Apathya&nbsp;affect its morbidity. Etiology, etiopathogenesis with clinical manifestation of Prameha are very well described in ancient textbook like Charaka Samhita, Sushruta Samhita, Astanga Hridyam and Astanga Samgraha etc. In all treatise of Ayurveda, Prameha is considered as Santarpanajanya Vikara that is of three types i.e., Vataj, Pittaj and Kaphaja. Madhumeha mentioned under the heading of Vataj Prameha that is closely correlated to diabetes mellitus. Diabetes Mellitus is of two type viz., Type-1 Diabetes &amp; Type-2 Diabetes. Among that, Type-2 diabetes is non-insulin dependent diabetes mellitus that occur in middle age. In this clinical entity insulin resistance was developed. In its management, Pathya–Apathya&nbsp;contributes a major role in controlling blood sugar level. Therefore, author attempted to highlight role of Pathya–Apathya&nbsp;(do’s &amp; don’t) in the management of Type-2 Diabetes Mellitus

A comparative study of syrup Trayodashanga Kashaya and syrup Vyaghradi Kashaya along with Anu Taila Nasya in the management of Vataj Pratishyaya W.S.R. to allergic rhinitis

Introduction: Allergic rhinitis (AR) is the most common disease among children which is mentioned in Ayurveda as “Pratishyaya”. This manifests when the Kapha, Rakta, and Pitta migrate from the root of the Nasa Pradesha and get stuck in Shirapradesha which is already vitiated by the Vata Dosha. There are five types of Pratishyaya described in the Ayurveda textbook, out of which Vataj Pratishyaya can be correlated with AR. Methods: Eighty-two children suffering from Vataj Pratishyaya were randomly selected and divided into four groups, namely Group A was treated with Compound A (syrup Trayodashanga Kashaya), Group B was treated with Compound A (syrup Trayodashanga Kashaya) and Anu Taila Nasya, Group C were treated with Compound A-1 (syrup Vyaghradi Kashaya), while Group D was treated with Compound A-1 (syrup Vyaghradi Kashaya) and Anu Taila Nasya. A total of 60 children completed the treatment, 15 in each group. Result: Group B showed extremely significant results in relieving signs and symptoms of Vataj Pratishyaya in comparison to Group A, C, and D. Conclusion: All four groups showed extremely significant results in subjective parameters while no changes were seen in objective parameters

Dexamethasone in children mechanically ventilated for lower Respiratory tract infection caused by respiratory syncytial Virus: a randomised controlled trial.

Objective:To determine the efficacy of dexamethasone in the treatment of mechanically ventilated children with respiratory syncytial virus–severe lower respiratory tract infection.Design:International, multicenter, randomized, double-blind, placebo-controlled trial.Setting:Twelve pediatric intensive care units.Subjects:Children (200 mm Hg and/or mean arterial pressure ≤10 cm H 2 O) and a noninferiority approach in those with severe oxygen abnormalities (PaO 2 /Fio 2 ≤200 mm Hg and mean arterial pressure >10 cm H 2 O). Primary outcome was the duration of mechanical ventilation. In the subgroup with mild oxygenation abnormalities, 45 of the 89 included patients received dexamethasone and 44 placebo; in the subgroup with severe oxygenation abnormalities, 28 of the 56 included patients received dexamethasone and 28 placebo. Baseline characteristics in both treatment arms were similar for both subgroups. After the third interim analysis, the trial was stopped early for futility taking the slow enrollment into account. At that time, the median duration (interquartile range) of mechanical ventilation was 137 (91–195) hrs in the dexamethasone- and 139 (117–188) hrs in the placebo-treated patients in the subgroup with mild oxygenation abnormalities ( p = .6). In the subgroup with severe oxygenation abnormalities, it was 171 (136–212) hrs in the dexamethasone- and 170 (125–201) hrs in the placebo-treated patients ( p = .6).Conclusion:In this prematurely ended trial in children mechanically ventilated for severe respiratory syncytial virus–lower respiratory tract infection, we found no evidence of a beneficial effect of dexamethasone in children with mild oxygenation abnormalities. Neither was evidence found that dexamethasone may prolong mechanical ventilation in those with severe oxygenation abnormalities.0info:eu-repo/semantics/publishe