Monitoring ground movement at Volcán de Colima, Mexico, using Sentinel-1 data and SqueeSAR®

Volcán de Colima is a highly active stratovolcano in western Mexico which presents a significant hazard to over 300,000 people who live within ca 40 km of the volcano. Due to its persistent activity, the volcano is actively monitored and researched, and understanding the patterns of behaviour is vital to accurate hazard assessment. Sentinel-1 SAR images from ascending and descending orbits allow 1D and 2D ground motions to be retrieved using multi-interferogram techniques. SqueeSAR®’s unique processing allows a better characterisation of subtle ground movements in remote, rural mountainous areas compared to many other multi-interferogram techniques. A dataset of 147 SAR scenes (2017-2019) has been processed to show patterns of lava subsidence (<150 mm of downward vertical deformation over 2 years), as well as volcano deflation and apparent westward lateral movement. These data indicate that viscous andesitic lava flows may remain mobile for years following eruption and emplacement, and that the entire volcanic edifice is subsiding. Despite the apparent quiescence, volcanic edifices can remain highly dynamic after the termination of explosive or effusive activity. We interpret that the western flank of Volcán de Colima may become steeper with time and may be of long-term concern for hazard assessment activities. Thematic collection: This article is part of the Remote sensing for site investigations on Earth and other planets collection available at: https://www.lyellcollection.org/topic/collections/remote-sensing-for-site-investigations-on-earth-and-other-planet

Functional polymorphisms in the P2X7 receptor gene are associated with stress fracture injury

Context: Military recruits and elite athletes are susceptible to stress fracture injuries. Genetic predisposition has been postulated to have a role in their development. The P2X7 receptor (P2X7R) gene, a key regulator of bone remodelling, is a genetic candidate that may contribute to stress fracture predisposition. Objective: To evaluate the putative contribution of P2X7R to stress fracture injury in two separate cohorts, military personnel and elite athletes. Methods: In 210 Israeli Defence Forces (IDF) military conscripts, stress fracture injury was diagnosed (n=43) based on symptoms and a positive bone scan. In a separate cohort of 518 elite athletes, self-reported medical imaging scan-certified stress fracture injuries were recorded (n=125). Non-stress fracture controls were identified from these cohorts who had a normal bone scan or no history or symptoms of stress fracture injury. Study participants were genotyped for functional SNPs within the P2X7R gene using proprietary fluorescence-based competitive allele-specific PCR assay. Pearson Chi-square (χ2) tests, corrected for multiple comparisons, were used to assess associations in genotype frequencies. Results: The variant allele of P2X7R SNP rs3751143 (Glu496Ala- loss of function) was associated with stress fracture injury, while the variant allele of rs1718119 (Ala348Thr- gain of function) was associated with a reduced occurrence of stress fracture injury in military conscripts (P<0.05). The association of the variant allele of rs3751143 with stress fractures was replicated in elite athletes (P<0.05), whereas the variant allele of rs1718119 was also associated with reduced multiple stress fracture cases in elite athletes (P<0.05). Conclusions: The association between independent P2X7R polymorphisms with stress fracture prevalence supports the role of a genetic predisposition in the development of stress fracture injury

Inter-relationship between microsatellite instability, thymidylate synthase expression, and p53 status in colorectal cancer: implications for chemoresistance

BACKGROUND: Studies indicate that thymidylate synthase (TS) expression, p53 and mismatch repair status have potential to influence colorectal cancer (CRC) outcome. There is, however, little data on the inter-relationship between these three markers. We sought to investigate whether relationships exist between these markers that might contribute to CRC phenotypes. METHODS: Four hundred and forty-one stage I-III CRCs were investigated. p53 status and TS expression were assessed by standard immunohistochemistry methods. Mismatch repair status was determined by assessment of microsatellite instability (MSI) using radiolabelled microsatellite genotyping. RESULTS: 244 tumours (55%) over-expressed p53, and 259 (58%) expressed high TS levels. 65 tumours (15%) had MSI. A significant relationship between p53 over-expression and high TS expression was observed (p = 0.01). This was independent of MSI status. A highly significant inverse relationship between MSI and p53 status was observed (p = 0.001). No relationship was seen between MSI status and TS expression (p = 0.59). CONCLUSION: Relationships exist between p53 status and TS expression, and MSI and p53 status. These inter-relationships may contribute to the clinical phenotype of CRCs associated with each of the molecular markers. High TS expression is unlikely to account for the clinical behaviour of CRCs with MSI

Large-scale GWAS identifies multiple loci for hand grip strength providing biological insights into muscular fitness

Hand grip strength is a widely used proxy of muscular fitness, a marker of frailty, and predictor of a range of morbidities and all-cause mortality. To investigate the genetic determinants of variation in grip strength, we perform a large-scale genetic discovery analysis in a combined sample of 195,180 individuals and identify 16 loci associated with grip strength (P<5 × 10−8) in combined analyses. A number of these loci contain genes implicated in structure and function of skeletal muscle fibres (ACTG1), neuronal maintenance and signal transduction (PEX14, TGFA, SYT1), or monogenic syndromes with involvement of psychomotor impairment (PEX14, LRPPRC and KANSL1). Mendelian randomization analyses are consistent with a causal effect of higher genetically predicted grip strength on lower fracture risk. In conclusion, our findings provide new biological insight into the mechanistic underpinnings of grip strength and the causal role of muscular strength in age-related morbidities and mortality.This research has been conducted using the UK Biobank Resource. The Fenland Study is supported by the UK Medical Research Council (MRC) (MC_UU_12015/1; MC_UU_12015/2; MC_UU_12015/3). EPIC-Norfolk is supported by the MRC (G401527, G1000143) and Cancer Research UK (A8257). The HCS is gratefully supported by the University of Newcastle (Australia) and the Fairfax Family Foundation. Sydney MAS is supported by the Australian National Health and Medical Research Council (NHMRC), grants ID568969, ID350833 and ID109308. Sydney MAS DNA was extracted by Genetic Repositories Australia, funded by NHMRC Enabling Grant 401184. The GEFOS Study, used as controls for the US and Jamaican athletes, was supported in part by NIH grants U01 HG004436 and P30 DK072488, and the Baltimore Geriatrics Research, Education, and Clinical Center of the Department of Veterans Affairs. The Novo Nordisk Foundation Center for Basic Metabolic Research is an independent Research Center at the University of Copenhagen partially funded by an unrestricted donation from the Novo Nordisk Foundation (www.metabol.ku.dk). TwinsUK was funded by the Wellcome Trust (WT), MRC, and European Union. The study also receives support from the National Institute for Health Research (NIHR) BioResource Clinical Research Facility and Biomedical Research Centre based at Guy's and St Thomas' NHS Foundation Trust and King's College London. SNP Genotyping was performed by The WT Sanger Institute and National Eye Institute via NIH/CIDR. M.McC is a WT Senior Investigator and receives support from WT 090532 and 098381. TW is the recipient of a studentship from MedImmune. Research by A. Lucia is supported by Fondo de Investigaciones Sanitarias and Fondos Feder (grant # PI15/0558). EM-M. was a recipient of a Grant-in-Aid for JSPS Fellow from the Japan Society for the Promotion of Science. This work was supported in part by grants from the Grant-in-Aid for Scientific Research (B) (15H03081 to NF) of the Japanese Ministry of Education, Culture, Sports, Science and Technology and by a grant-in-aid for scientific research (to M. Miyachi) from the Japanese Ministry of Health, Labor, and Welfare. This work was further supported by NIH grants R01 AR41398 and U24 AG051129

Long-period seismicity during magma movement at Volcan de Colima

During the period from February to September 2005, Volcan de Colima produced 30 Vulcanian explosions of sufficient magnitude to produce pyroclastic flows of variable size, with a total volume of at least 2.5 x 10(6) m(3). Swarms of long-period events were associated with each event, their duration ranging from about 6 h to 3 days and each swarm containing up to 886 events. The characteristics of the swarms have been studied to understand the source mechanism and their relationship with the Vulcanian explosions. In total, 12,548 long-period events were analysed using various comparative and statistical methods. Patterns were not apparent in the data with no correlation between different properties of the swarms (duration, magnitude or frequency of occurrence of LP events) and the magnitude of the associated Vulcanian explosion, whether recorded by seismicity, volume of pyroclastics or altitude of the eruption column. This, along with other characteristics of the swarms, such as the continuation of the swarm after the explosion, with an increase in long-period event amplitude in some cases, suggests that the mechanism is not merely associated with the pressurization under an impermeable cap and resulting pressure differentials between adjacent volumes within the system. It is more likely that the production of long-period events is dominated by brittle fracturing on the margins of an ascending magma body. A model is proposed whereby the unloading above the ascending magma column produced by a Vulcanian explosion resulted in an increase in ascent rate, reflected in the increasing amplitude of long-period events. The results reflect the complexity of non-linear processes involved during magma ascent, degassing, crystallization and rupture of the impermeable plug during the Vulcanian process. At Volcan de Colima, as at many volcanoes, long-period events represent a useful precursor for eruptive activity. For monitoring, this paper highlights some useful analyses that can be carried out, which could illustrate certain characteristics of an eruptive episode. A preliminary model is presented of the conduit processes at work during the cyclic extrusive and explosive activity during 2005