89 research outputs found
The SNARE Protein Syntaxin 3 Confers Specificity for Polarized Axonal Trafficking in Neurons.
Cell polarity and precise subcellular protein localization are pivotal to neuronal function. The SNARE machinery underlies intracellular membrane fusion events, but its role in neuronal polarity and selective protein targeting remain unclear. Here we report that syntaxin 3 is involved in orchestrating polarized trafficking in cultured rat hippocampal neurons. We show that syntaxin 3 localizes to the axonal plasma membrane, particularly to axonal tips, whereas syntaxin 4 localizes to the somatodendritic plasma membrane. Disruption of a conserved N-terminal targeting motif, which causes mislocalization of syntaxin 3, results in coincident mistargeting of the axonal cargos neuron-glia cell adhesion molecule (NgCAM) and neurexin, but not transferrin receptor, a somatodendritic cargo. Similarly, RNAi-mediated knockdown of endogenous syntaxin 3 leads to partial mistargeting of NgCAM, demonstrating that syntaxin 3 plays an important role in its targeting. Additionally, overexpression of syntaxin 3 results in increased axonal growth. Our findings suggest an important role for syntaxin 3 in maintaining neuronal polarity and in the critical task of selective trafficking of membrane protein to axons
Galaxy Zoo: Disentangling the Environmental Dependence of Morphology and Colour
We analyze the environmental dependence of galaxy morphology and colour with
two-point clustering statistics, using data from the Galaxy Zoo, the largest
sample of visually classified morphologies yet compiled, extracted from the
Sloan Digital Sky Survey. We present two-point correlation functions of spiral
and early-type galaxies, and we quantify the correlation between morphology and
environment with marked correlation functions. These yield clear and precise
environmental trends across a wide range of scales, analogous to similar
measurements with galaxy colours, indicating that the Galaxy Zoo
classifications themselves are very precise. We measure morphology marked
correlation functions at fixed colour and find that they are relatively weak,
with the only residual correlation being that of red galaxies at small scales,
indicating a morphology gradient within haloes for red galaxies. At fixed
morphology, we find that the environmental dependence of colour remains strong,
and these correlations remain for fixed morphology \textit{and} luminosity. An
implication of this is that much of the morphology--density relation is due to
the relation between colour and density. Our results also have implications for
galaxy evolution: the morphological transformation of galaxies is usually
accompanied by a colour transformation, but not necessarily vice versa. A
spiral galaxy may move onto the red sequence of the colour-magnitude diagram
without quickly becoming an early-type. We analyze the significant population
of red spiral galaxies, and present evidence that they tend to be located in
moderately dense environments and are often satellite galaxies in the outskirts
of haloes. Finally, we combine our results to argue that central and satellite
galaxies tend to follow different evolutionary paths.Comment: 19 pages, 18 figures. Accepted for publication in MNRA
Galaxy Zoo: The large-scale spin statistics of spiral galaxies in the Sloan Digital Sky Survey
We re-examine the evidence for a violation of large-scale statistical
isotropy in the distribution of projected spin vectors of spiral galaxies. We
have a sample of spiral galaxies from the Sloan Digital Sky
Survey, with their line of sight spin direction confidently classified by
members of the public through the online project Galaxy Zoo. After establishing
and correcting for a certain level of bias in our handedness results we find
the winding sense of the galaxies to be consistent with statistical isotropy.
In particular we find no significant dipole signal, and thus no evidence for
overall preferred handedness of the Universe. We compare this result to those
of other authors and conclude that these may also be affected and explained by
a bias effect.Comment: Accepted for publication in MNRAS. 8 pages, 5 figure
Galaxy Zoo: Motivations of Citizen Scientists
Citizen science, in which volunteers work with professional scientists to
conduct research, is expanding due to large online datasets. To plan projects,
it is important to understand volunteers' motivations for participating. This
paper analyzes results from an online survey of nearly 11,000 volunteers in
Galaxy Zoo, an astronomy citizen science project. Results show that volunteers'
primary motivation is a desire to contribute to scientific research. We
encourage other citizen science projects to study the motivations of their
volunteers, to see whether and how these results may be generalized to inform
the field of citizen science.Comment: 41 pages, including 6 figures and one appendix. In press at Astronomy
Education Revie
Galaxy Zoo: Exploring the Motivations of Citizen Science Volunteers
The Galaxy Zoo citizen science website invites anyone with an Internet
connection to participate in research by classifying galaxies from the Sloan
Digital Sky Survey. As of April 2009, more than 200,000 volunteers had made
more than 100 million galaxy classifications. In this paper, we present results
of a pilot study into the motivations and demographics of Galaxy Zoo
volunteers, and define a technique to determine motivations from free responses
that can be used in larger multiple-choice surveys with similar populations.
Our categories form the basis for a future survey, with the goal of determining
the prevalence of each motivation.Comment: 15 pages, 3 figure
Galaxy Zoo: Reproducing Galaxy Morphologies Via Machine Learning
We present morphological classifications obtained using machine learning for
objects in SDSS DR6 that have been classified by Galaxy Zoo into three classes,
namely early types, spirals and point sources/artifacts. An artificial neural
network is trained on a subset of objects classified by the human eye and we
test whether the machine learning algorithm can reproduce the human
classifications for the rest of the sample. We find that the success of the
neural network in matching the human classifications depends crucially on the
set of input parameters chosen for the machine-learning algorithm. The colours
and parameters associated with profile-fitting are reasonable in separating the
objects into three classes. However, these results are considerably improved
when adding adaptive shape parameters as well as concentration and texture. The
adaptive moments, concentration and texture parameters alone cannot distinguish
between early type galaxies and the point sources/artifacts. Using a set of
twelve parameters, the neural network is able to reproduce the human
classifications to better than 90% for all three morphological classes. We find
that using a training set that is incomplete in magnitude does not degrade our
results given our particular choice of the input parameters to the network. We
conclude that it is promising to use machine- learning algorithms to perform
morphological classification for the next generation of wide-field imaging
surveys and that the Galaxy Zoo catalogue provides an invaluable training set
for such purposes.Comment: 13 Pages, 5 figures, 10 tables. Accepted for publication in MNRAS.
Revised to match accepted version
Galaxy Zoo 1 : Data Release of Morphological Classifications for nearly 900,000 galaxies
Morphology is a powerful indicator of a galaxy's dynamical and merger
history. It is strongly correlated with many physical parameters, including
mass, star formation history and the distribution of mass. The Galaxy Zoo
project collected simple morphological classifications of nearly 900,000
galaxies drawn from the Sloan Digital Sky Survey, contributed by hundreds of
thousands of volunteers. This large number of classifications allows us to
exclude classifier error, and measure the influence of subtle biases inherent
in morphological classification. This paper presents the data collected by the
project, alongside measures of classification accuracy and bias. The data are
now publicly available and full catalogues can be downloaded in electronic
format from http://data.galaxyzoo.org.Comment: Accepted by MNRAS, 14 pages. Updated to match final version; problem
with table 7 header fixed. Full tables available at http://data.galaxyzoo.or
Stellar Diameters and Temperatures II. Main Sequence K & M Stars
We present interferometric diameter measurements of 21 K- and M- dwarfs made
with the CHARA Array. This sample is enhanced by literature radii measurements
to form a data set of 33 K-M dwarfs with diameters measured to better than 5%.
For all 33 stars, we compute absolute luminosities, linear radii, and effective
temperatures (Teff). We develop empirical relations for \simK0 to M4 main-
sequence stars between the stellar Teff, radius, and luminosity to broad-band
color indices and metallicity. These relations are valid for metallicities
between [Fe/H] = -0.5 and +0.1 dex, and are accurate to ~2%, ~5%, and ~4% for
Teff, radius, and luminosity, respectively. Our results show that it is
necessary to use metallicity dependent transformations to convert colors into
stellar Teffs, radii, and luminosities. We find no sensitivity to metallicity
on relations between global stellar properties, e.g., Teff-radius and
Teff-luminosity. Robust examinations of single star Teffs and radii compared to
evolutionary model predictions on the luminosity-Teff and luminosity-radius
planes reveals that models overestimate the Teffs of stars with Teff < 5000 K
by ~3%, and underestimate the radii of stars with radii < 0.7 R\odot by ~5%.
These conclusions additionally suggest that the models overestimate the effects
that the stellar metallicity may have on the astrophysical properties of an
object. By comparing the interferometrically measured radii for single stars to
those of eclipsing binaries, we find that single and binary star radii are
consistent. However, the literature Teffs for binary stars are systematically
lower compared to Teffs of single stars by ~ 200 to 300 K. Lastly, we present a
empirically determined HR diagram for a total of 74 nearby, main-sequence, A-
to M-type stars, and define regions of habitability for the potential existence
of sub-stellar mass companions in each system. [abridged]Comment: 73 pages, 12 Tables, 18 Figures. Accepted for publication in The
Astrophysical Journa
Revisiting Delta Y/Delta Z from multiple main sequences in Globular Clusters: insight from nearby stars
For nearby K dwarfs, the broadening of the observed Main Sequence at low
metallicities is much narrower than expected from isochrones with the standard
helium-to-metal enrichment ratio DY/DZ=2. Though the latter value fits well the
Main Sequence around solar metallicity, and agrees with independent
measurements from HII regions as well as with theoretical stellar yields and
chemical evolution models, a much higher DY/DZ~10 is necessary to reproduce the
broadening observed for nearby subdwarfs. This result resembles, on a milder
scale, the very high DY/DZ estimated from the multiple Main Sequences in Omega
Cen and NGC 2808. Although not "inverted" as in omega Cen, where the metal-rich
Main Sequence is bluer than the metal-poor one, the broadening observed for
nearby subdwarfs is much narrower than stellar models predict for a standard
helium content. We use this empirical evidence to argue that a revision of
lower Main Sequence stellar models, suggested from nearby stars, could
significantly reduce the helium content inferred for the subpopulations of
those globular clusters. A simple formula based on empirically calibrated
homology relations is constructed, for an alternative estimate of DY/DZ in
multiple main sequences. We find that, under the most favourable assumptions,
the estimated helium content for the enriched populations could decrease from
Y~0.4 to as low as Y~0.3.Comment: 15 pages, 12 figures, in press on MNRA
Galaxy Zoo: Dust in Spirals
We investigate the effect of dust on spiral galaxies by measuring the
inclination-dependence of optical colours for 24,276 well-resolved SDSS
galaxies visually classified in Galaxy Zoo. We find clear trends of reddening
with inclination which imply a total extinction from face-on to edge-on of 0.7,
0.6, 0.5 and 0.4 magnitudes for the ugri passbands. We split the sample into
"bulgy" (early-type) and "disky" (late-type) spirals using the SDSS fracdeV (or
f_DeV) parameter and show that the average face-on colour of "bulgy" spirals is
redder than the average edge-on colour of "disky" spirals. This shows that the
observed optical colour of a spiral galaxy is determined almost equally by the
spiral type (via the bulge-disk ratio and stellar populations), and reddening
due to dust. We find that both luminosity and spiral type affect the total
amount of extinction, with "disky" spirals at M_r ~ -21.5 mags having the most
reddening. This decrease of reddening for the most luminous spirals has not
been observed before and may be related to their lower levels of recent star
formation. We compare our results with the latest dust attenuation models of
Tuffs et al. We find that the model reproduces the observed trends reasonably
well but overpredicts the amount of u-band attenuation in edge-on galaxies. We
end by discussing the effects of dust on large galaxy surveys and emphasize
that these effects will become important as we push to higher precision
measurements of galaxy properties and their clustering.Comment: MNRAS in press. 25 pages, 22 figures (including an abstract comparing
GZ classifications with common automated methods for selecting disk/early
type galaxies in SDSS data). v2 corrects typos found in proof
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